POSTHARVEST BIOLOGY AND TECHNOLOGY
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2017
·
IF 3.248
Shiwen Dou,Qiuli Ouyang,Kuiyi You,Jingjing Qian,Nengguo Tao
Thymol is a naturally occurring volatile terpenoid in plants that possesses strong antifungal activities against many pathogens. In this research, thymol was included into β -cyclodextrin and the antifungal activity of the resulting complex against Geotrichum citri-aurantii, the causal agent of sour rot in citrus fruit, was investigated. The inhibition concentration of the β -cyclodextrin-thymol inclusion complex ( β -CTIC) to G. citri-aurantii was estimated to be 4.0 g L −1 , which was supposed to be a comparable antifungal efficiency of 0.5 g L −1 thymol alone. The structure and characteristic of β -CTIC were evaluated by scanning electron microscopy (SEM), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and entrapment efficiency (EE). The ROESY spectrum of β -CTIC showed appreciable correlation of H-1, H-4 and H-3 protons of thymol with the H-3′, H-5′ and H-6′ protons of β -cyclodextrin, respectively. In addition, β -CTIC was found to reduce the decay of citrus fruit inoculated with G. citri-aurantii and maintain the fruit quality. Our present study suggested that β -CTIC might be used as an alternative to chemical fungicides in controlling the postharvest sour rot of citrus fruit.
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高影响因子
CHEMISTRY OF MATERIALS
·
2017
·
IF 9.466
Hao Wu,Zhimin Chen,Jialin Zhang,Feng Wu,Chunying He,Zhiyu Ren,Yiqun Wu
Manipulating the morphology and protonic acid doping of polyaniline (PANI) is significant for optimizing its NH3-sensing. Herein, tetra-β-carboxyphthalocyanine cobalt(II) (TcPcCo) acted as the dopant and structure-directing agent simultaneously to fabricate the uniform fibrous network-like PANI (PANI-TcPcCo hybrids) by a one-step polymerization at low temperature. During the reaction process, the protonic acid groups in TcPcCo not only induced the aniline monomers polymerizing into one-dimensional nanofibers (consist of both solid and hollow cylinders) with abundant tiny protuberances on the surface but also successfully doped into PANI. The resulting PANI-TcPcCo hybrids displayed the enhancement in terms of the good conductivity, the large gas adsorption capacity, and the unobstructed channels for the electron and gas transport. The central metal atoms of TcPcCo present the strong and selective affinity to NH3. Meanwhile, the deep-seated conversion of PANI’s molecular structure after exposure in NH3 could occur due to the presence of TcPcCo. Thus, the PANI-2.5TcPcCo sensor showed the excellent NH3-sensing performance at room temperature, including an ultrahigh and fast response (802.7% and ∼17.0 s for 100 ppm of NH3), a very low detection limit of 10 ppb (about 5000 parts of human olfaction limit of detection, 55 ppm), and superior NH3-sensing stability and selectivity. The strategy developed here provides a reliable and valid way to synthesize functional PANI-based hybrids with unique morphology and appropriate doping, which are able to be extended to other areas.
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Heliyon
·
2024
·
IF 3.4
Youwu Wen,Jiajia Teng,An Liu
This study concentrated on developing a novel sprayable hydrogel by the combination of a thermosensitive hydrogel (Pluronic F-127, PF-127) and an inclusion complex of glabridin (GLD) and sulfobutylether-β-cyclodextrin (SBE-β-CD). GLD was encapsulated into SBE-β-CD by the freeze-drying method. The successful encapsulation of the inclusion complex was verified by Fourier transform infrared (FT-IR), X-ray diffractometer (XRD), scanning electron microscope (SEM), and nuclear magnetic resonance spectroscopy (NMR). The prepared GLD/SBE-β-CD inclusion complex exhibited 93.24%～96.46% encapsulation efficiency and 11.24%～11.93% loading capacity, and GLD/SBE-β-CD showed excellent biocompatibility on human vascular endothelial cells (HUVECs) and erythrocytes. GLD/SBE-β-CD inclusion complex exerts higher antioxidant and antibacterial activity than GLD alone. The GLD/SBE-β-CD inclusion complex was well dispersed within 20% PF-127 solution to form the PF-127/GLD/SBE-β-CD hydrogel. Introducing the inclusion complex into the PF-127 hydrogel did not influence its loose and porous structure, while PF-127/GLD/SBE-β-CD hydrogel demonstrated controlled GLD release in a sustained manner. The full-thickness skin wound model showed that the PF-127/GLD/SBE-β-CD hydrogel accelerated the healing of traumatic skin defects. In one aspect, the porous and biocompatible structure of the hydrogel provides favorable conditions for skin cell adhesion and proliferation. In another respect, GLD in therapeutic hydrogel markedly promotes the angiogenesis of endothelial cells, and the migration of keratinocytes. It also presents excellent antibacterial properties in wound surface. Taken together, this work established a novel sprayable hydrogel (PF-127/GLD/SBE-β-CD) and proved that it is an effective strategy for traumatic wound healing.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2024
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IF 4.4
Hui-Zhen Yang,Jia-Jia Chen,Lan Zhang,Xiao-Li Tian,Rong Wang,Lin Pu,Xiao-Qi Yu,Ji Zhang
Nitric oxide (NO) / β-Lapachone (Lap) combined therapy by causing oxidative stress is an effective tumor therapy strategy. Herein, a dual-responsive lipid nanoparticles (LNPs) LSNO for NO / Lap co-delivery were constructed from the zinc-coordinated lipid ( DSNO(Zn) ) and the hydrophobic drug Lap in the presence of helper lipids (DOPE and DSPE-PEG 2000 ). The zinc-coordinated structure in LSNO might elevate the Zn 2+ content in tumor cells, contributing to antioxidant imbalance. The fluorescent assays proved the light-triggered NO release and fluorescent self-reporting abilities of LSNO . In addition, the LNPs had good drug release behavior under high concentration of GSH, indicating the NO / drug co-delivery capacity. In vitro antitumor assays showed that the NO / Lap combination treatment group could induce more significant tumor cell growth inhibition and cell apoptosis than individual NO or Lap treatment. The following mechanism studies revealed that NO / Lap combination treatment led to distinct oxidative stress by producing reactive oxygen species (ROS) and peroxynitrite anion (ONOO – ). On the other hand, the intracellular redox balance could be further disrupted by Lap-induced NADPH consumption and Zn 2+ / NO-induced reductase activities downregulation, thus promoting the degree of cell damage. Besides, it was also found that NO and Lap could directly damage nuclear DNA and induce mitochondrial dysfunction, thereby leading to caspase-3 activation and tumor cell death. These results proved that LSNO could serve as a promising multifunctional tumor therapy platform.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiaobi Fang,Xiaojing Chen,Wei Dong,Fan Ye
In the management of diabetic oral ulcers, current treatments frequently fall short due to complications from bacterial contamination, oxidative damage, and impaired angiogenesis. These shortcomings highlight the pressing need for more efficacious interventions that not only circumvent these issues but also expedite healing processes. To bridge this gap, we have introduced a novel hydrogel patch, POQ2 [a poly(tannic acid) particle-incorporated oxidized β-glucan/quaternized chitosan hydrogel]. POQ2 demonstrated significant improvements in healing outcomes by enhancing bacterial clearance (up to 99.7 % against Escherichia coli and 99.2 % against Staphylococcus aureus ), reducing oxidative stress through efficient ROS scavenging (70.2 % reduction in DPPH radicals), and promoting angiogenesis. In vivo experiments on diabetic rat models showed accelerated wound closure and reduced IL-6 inflammatory markers, with nearly complete ulcer healing within seven days. The introduction of POQ2 into clinical protocols represents a substantial advancement in the management of diabetic oral ulcers, promising not only improved patient outcomes but also a shift in the standard of care in this challenging clinical area. This innovation not only fills a significant gap in diabetic wound care but also sets a new benchmark for the development of future treatments, potentially influencing broader therapeutic strategies for managing complex diabetic wounds.
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高影响因子
Pharmaceutics
·
2024
·Vol. 16
·期 1
·154
·
IF 4.9
Xueqin Gao,Dirong Dong,Chong Zhang,Yuxing Deng,Jiahui Ding,Shiqi Niu,Songwei Tan,Lili Sun
Gene therapy displays great promise in the treatment of cervical cancer. The occurrence of cervical cancer is highly related to persistent human papilloma virus (HPV) infection. The HPV oncogene can be cleaved via gene editing technology to eliminate carcinogenic elements. However, the successful application of the gene therapy method depends on effective gene delivery into the vagina. To improve mucosal penetration and adhesion ability, quaternized chitosan was introduced into the poly(β-amino ester) (PBAE) gene-delivery system in the form of quaternized chitosan-g-PBAE (QCP). At a mass ratio of PBAE:QCP of 2:1, the polymers exhibited the highest green fluorescent protein (GFP) transfection efficiency in HEK293T and ME180 cells, which was 1.1 and 5.4 times higher than that of PEI 25 kD. At this mass ratio, PBAE–QCP effectively compressed the GFP into spherical polyplex nanoparticles (PQ–GFP NPs) with a diameter of 255.5 nm. In vivo results indicated that owing to the mucopenetration and adhesion capability of quaternized CS, the GFP transfection efficiency of the PBAE–QCP hybrid system was considerably higher than those of PBAE and PEI 25 kD in the vaginal epithelial cells of Sprague–Dawley rats. Furthermore, the new system demonstrated low toxicity and good safety, laying an effective foundation for its further application in gene therapy.Keywords:gene delivery;poly(β-amino ester);chitosan;mucoadhesion
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JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 2
·e70056
·
IF 3.4
Yihan Wang,Kai Wang,Xiaofeng Xiang,Shuming Zhang,Jie Huang,Chunhong Tang,Qiang Wang
The high cholesterol content of beef tallow is one of the potential risks affecting the nutritional value and safety of beef tallow hotpot. In this study, a novel pectin-crosslinked β-cyclodextrin (β-CD) complex was successfully prepared by the cross-linking method to reduce the cholesterol content in beef tallow. When 3% (by mass of beef tallow) of the pectin-crosslinked β-CD
complex was added, the removal rate of cholesterol in beef tallow reached 73.43%, which was significantly higher than that of the currently known materials, including β-CD. Moreover, the indexes such as fatty acid composition, chromaticity, and aroma components of beef tallow before and after cholesterol removal were measured. The results showed that the fatty acid composition and chromaticity value of beef tallow treated with the pectin-crosslinked β-CD complex did not change significantly, but the contents of aromatic components, aldehydes, ketones, and alcohol flavor compounds in beef tallow increased significantly. Through characterization experiments, it was confirmed that compared with β-CD, the pectin-crosslinked β-CD complex required a higher temperature to reach the maximum thermal decomposition rate, indicating that its structure had higher thermal stability and could effectively remove cholesterol under high-temperature conditions. In addition, after the nematode toxicity experiment, there was still a survival rate of 97.97%, indicating that the pectin-crosslinked β-CD complex could effectively reduce the cholesterol content in beef tallow without toxicity. These results suggest that the pectin-crosslinked β-CD complex has great potential as an adsorbent for removing cholesterol from animal fats and oils in the food industry.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 7
·3118
·
IF 4.9
Guotao Mao,Ming Song,Hao Li,Junhan Lin,Kai Wang,Qian Liu,Zengping Su,Hongsen Zhang,Lijuan Su,Hui Xie,Andong Song
The enzymatic hydrolysis of lignocellulose is often hindered by the glucose-mediated inhibition of β-glucosidases, a major bottleneck in industrial cellulose degradation. Identifying novel glucose-tolerant β-glucosidases is essential for enhancing saccharification efficiency. In this study, we cloned and heterologously expressed a novel β-glucosidase, RpBgl8, from the termiteReticulitermes perilucifugusinEscherichia coli. Sequence and structural analyses classified RpBgl8 as a glycoside hydrolase family 1 enzyme. The purified enzyme exhibited optimal activity at 45 °C and pH 7.0, with broad stability across pH 4.0–8.0. Notably, RpBgl8 demonstrated high tolerance to lignocellulose-derived inhibitors and organic solvents, maintaining 100% activity in 15% ethanol. Furthermore, RpBgl8 exhibited outstanding glucose tolerance, retaining 100% activity at 2.5 M glucose and 82% activity at 4.0 M glucose—outperforming most previously reported β-glucosidases. A structural analysis revealed a narrow, hydrophobic substrate pocket, with residue F124 at the glycone-binding site critical for minimizing glucose accumulation. The F124W mutation significantly reduced glucose tolerance, confirming that hydrophobic interactions at the active site mitigate inhibition. These findings establish RpBgl8 as a promising candidate for high-solid biomass processing and simultaneous saccharification and fermentation applications, highlighting termites as underexplored sources of biocatalysts with unique industrial potential.
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高影响因子
DESALINATION
·
2024
·
IF 8.3
Shao-Lu Li,Dandan Cheng,Niamat Ullah,Youcai Chen,XinMeng Wang,Mengfan Wang,Qiyu Duan,Genghao Gong,Yunxia Hu
Graphene oxide (GO) membrane is considered as one of the preferred choice for textile wastewater treatment thanks to its unique 2D interlayer channel features. However, GO laminar stacking is inclined to water-induced structure instability, and the narrow interlayer spacing limit mass transport efficiency, hampering its effectiveness in water treatment applications. Herein, a layer-by-layer stacked microcomposite membrane based on GO and highly microporous β-cyclodextrin based nanosheets (β-CDNS) served as intercalator was developed. The distinctive layered structure was further stabilized by covalent cross-linking with glutaraldehyde (GA). The microporous β-CDNS offered additional nanochannels that facilitated the rapid penetration of water molecules, while the abundant -OH groups provided sites for chemical cross-linking, enhancing membrane stability. The optimal GO/β-CDNS1 membrane exhibited a superior water permeance of up to 83.5 LMH/bar, with >99 % rejection for dyes such as Congo red (CR), methyl blue (MB), and chrome black T (CBT), 95.5 % for acid fuchsin (AF). Meanwhile, the GO/β-CDNS1 membrane demonstrated ultra-low (<5 %) retention for inorganic salts (MgSO 4 , MgCl 2 and NaCl), with a separation factor as high as 194 for MB/NaCl mixtures. Therefore, employing functional interlayer nano-additives to well regulate the GO interlayer structure offers a promising guideline for developing high-performance GO-based membranes.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Liuxi Xia,Yuxiang Bai,Wanmeng Mu,Jinpeng Wang,Xueming Xu,Zhengyu Jin
Instead of β-cyclodextrin (β-CD), branched β-CDs have been increasingly used in many aspects as they possess better solubility and higher bioadaptability. But most commercialized branched β-CDs were chemically synthesized. Thus, the glucosyl-β-cyclodextrin (G1-β-CD) prepared via enzymatic approach could be a nice substitute. However, the yield of G1-β-CD was low. Here, we reported a controlled two-step reaction to efficiently prepare G1-β-CD from maltodextrins by β-cyclodextrin glucosyltransferase (β-CGTase) and amyloglucosidase (AG). Compared to the single β-CGTase reaction, controlled two-step reaction caused a yield increase of G1-β-CD by 130%. Additionally, the percentage of G1-β-CD was enhanced from 2.4% to 24.0% and the side products α-CD and γ-CD were hydrolyzed because of the coupling activity of β-CGTase. Thus, this controlled two-step reaction might be an efficient approach for industrial production of pure G1-β-CD.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Mai Luo,Yifan Hua,Yiran Liang,Jiajun Han,Donghui Liu,Wenting Zhao,Peng Wang
A novel functionalized carbon dot has been synthesized by covalently linking β-cyclodextrin to the surface of N, S codoped carbon dots (β-CD-CDs). The characterization was confirmed by transmission electron microscopy , X-ray photoelectron spectroscopy, infrared spectra, ultraviolet-visible, and fluorescence emission spectra. On the basis of this carbon dot and (ferrocenylmethyl) trimethylammonium iodide (Fc + ), a photo-induced electron transfer (PET) fluorescent probe system was developed to determine the concentration of testosterone in water and identify testosterone in cell by fluorescence imaging as a visible biomarker. Under the optimum condition, the fluorescent intensity of the probe system linearly responded to the concentration of testosterone from 0 μM to 280 μM and the limit of detection was 0.51 μM. This probe system also performed well at determining testosterone in groundwater with average recoveries of testosterone ranging from 96% to 107% at spiking levels of 0.5–100 μM, and the relative standard deviation remained below 13%, which provided a reliable, rapid and easy method to determine testosterone in environmental water. Furthermore, the low cytotoxicity, high anti-interference ability, and excellent biocompatibility of β-CD-CDs made this probe system successfully used in cell fluorescence imaging to monitor levels of testosterone in the cytoplasm of cells with a promising application value in medical research.
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COMPOSITES SCIENCE AND TECHNOLOGY
·
2016
·
IF 3.897
Weichang Li,Junpei Kang,Yulin Yuan,Fengcai Xiao,Hang Yao,Sa Liu,Jianxi Lu,Yingjun Wang,Zhen Wang,Li Ren
The poly(vinyl alcohol) (PVA) hydrogel is regarded as a potential articular cartilage replacement for its good biocompatibility , high permeability to fluid and load-bearing properties. This work investigated a novel Poly(vinyl alcohol)-polyetheretherketone/Poly(vinyl alcohol)-β-tricalcium phosphate (PVA-PEEK/PVA-β-TCP) bilayered hydrogels by freezing-thawing with biomimetic properties for articular cartilage and subchondral bone is developed. The bilayered hydrogels microarchitecture consists of a highly porous and dense structure, and the internal structure were analyzed by micro-CT. The morphology of the resulting hydrogels was analyzed by scanning electron microscopy (SEM), the enhancement of the mechanical properties of the PVA-PEEK/PVA-β-TCP bilayered hydrogels were demonstrated by mechanical testing. The bilayered structure indicate that a good bonding exist between the two layers, which is known to be a requisite necessary to assure a good integrity and functionality of the osteochondral construct. In addition, in vitro cell culture studies revealed that the hydrogels has no negative effect on the cell viability and proliferation and possess good biocompatibility. Then, the bilayered hydrogels were implanted into the knee joint defect of rabbits and hematoxylin and eosin and immunohistochemical staining. The PVA-PEEK/PVA-β-TCP bilayered hydrogels show good potential for use in the field of articular cartilage repair.
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危险品化学品经营许可证（带存储）