Foods
·
2025
·Vol. 14
·期 14
·2437
·
IF 5.1
Yongtai Wu,Bei Hu,Yuxin Wen,Zuowei Xiao,Lin Li,Xia Zhang,Zhenhui Zhang,Bing Li
Soy sauce (SS) is one of the most popular condiments in the world. However, Nε-carboxymethyl-lysine (CML) and 5-hydroxymethylfurfural (5-HMF), harmful Maillard reaction products, are present in SS. Worse still, their primary sources in SS production remain unclear, and their contents increase during the consumption of heated SS. In this study, CML and 5-HMF were simultaneously monitored, and thermal treatment and the addition of natural product were used to simultaneously reduce their contents during SS production and consumption. During SS production, CML and 5-HMF primarily originated from the raw materials used in SS production, Maillard reactions during fermentation, and the addition of food additives. Also, CML and 5-HMF were simultaneously found in commercial light soy sauce, dark soy sauce, and infant SS, and thermal treatment could increase their contents. Fortunately, additional thermal treatment of semi-finished SS (especially raw sauce and rude light SS) and appropriate concentrations of (−)-epicatechin (100 μM) and ascorbic acid (5 μM), respectively, added to SS for direct and heated consumption, could simultaneously reduce the CML and 5-HMF contents. This study highlights the presence of CML and 5-HMF in SS and proposes practical methods to simultaneously minimize their contents during production and consumption.
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高影响因子
CELLULOSE
·
2025
·
IF 4.8
Liu Wanni,Shi Xian,Liu Dongqi,Xing Xinyi,Ruan Mengya,Sun Song,Lyu Gaojin,Xu Siquan
Converting glucose-based carbohydrates such as microcrystalline cellulose and cellulose components in lignocellulose into 5-hydroxymethylfurfural (HMF) is a feasible way to achieve high-value utilization of biomass. To this end, this work assembled a series of bifunctional (x)Hf/SBA-15-S catalysts by introducing active hafnium species and grafting sulfonic acid groups onto the mesoporous SBA-15 silica carrier to drive the conversion of glucose, cellulose and untreated wheat straw into HMF. Through detailed parameter evaluation, competitive HMF yields were achieved from glucose (74%) and cellulose (51%), respectively. More importantly, the cellulose and hemicellulose components in untreated wheat straw could be efficiently converted simultaneously to obtain HMF and furfural with yields of 50 and 30%, respectively. Systematic characterization revealed that (x)Hf/SBA-15-S catalysts had an ordered pore channel, bifunctional acidity and good hydrothermal stability, which were beneficial to the process of cellulose undergoing depolymerization, isomerization and dehydration to produce HMF. Therefore, it is believed that the catalytic transformation strategy presented in this study has application prospects for realizing the utilization of glucose-based carbohydrates.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Gahlot Himanshi,Kang Sun Chul
Synergistic therapy is unwaveringly developing as a potential approach to enhance the effectiveness of anticancer medications in chemotherapy. Incorporating adjuvants alongside conventional anticancer medications has demonstrated selective cytotoxicity toward cancer cells while mitigating adverse effects such as DNA damage and systemic toxicity in normal cells. The extended use of 5-FU, a synthetic medicine used in chemotherapy, has many adverse effects, which limit its long-term use in clinical settings. Numerous investigations have demonstrated that luteolin exhibits anticancer effects and alleviates inflammation in mammalian cells. In this study, we provide the first experimental evidence of the synergistic interaction between luteolin and 5-FU in murine melanoma (B16F10) cells, compared to monotherapies (luteolin and 5-FU alone). Using MTT assay for cell viability, flow cytometry for cell cycle analysis, and Western blotting for apoptosis markers, we demonstrate that simultaneous exposure to luteolin and 5-FU speeds up oxidative stress and enhances the production of endogenous ROS. The combination therapy induced the G1-phase cell cycle arrest (65.39%) and decreased the cell viability of B16F10 cells to 34.6%. This, in turn, activates the DNA damage response and initiates the apoptotic pathway by suppressing autophagy and the DNA repair system. Further, the co-treatment of luteolin and 5-FU significantly decreases the mitochondrial membrane potential and reduces ATP production. The results indicate that the combination of luteolin and 5-FU may improve treatment efficacy while potentially decreasing the necessary dosage of 5-FU, therefore reducing its toxicity in clinical settings.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Huifang Zhao,Xiunan Xu,Mi Shi,Yi Chen,Bowei Guan,Fujuan Peng,Jinze Li,Yuanyuan Zhao,Lu Zhang,Ruiping Zhang
Spatial lipidomics analysis of three-dimensional (3D) tumor cell spheroids enables in-situ investigation of metabolic reprogramming across distinct regions cellular regions within a single spheroid. However, it is still a challenge to study the effect of 5-fluorouracil (5-FU) with 3D HepG2 spheroid on the alteration of abundance and spatial distribution of endogenous lipid species. Herein, we systematically optimized the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) methodologies for extending spatial lipidomics to in-situ screening and visualizing lipid changes in 3D HepG2 spheroids treated with 5-FU at the microregional level. The embedding protocols in carboxymethyl cellulose (CMC), gelatin, and optimal cutting temperature (OCT) compound of 3D HepG2 spheroid were evaluated for MALDI MSI analysis with 10 μm spatial resolution, respectively. Subsequently, CMC embedding of 3D HepG2 spheroid completely preserved the 3D spatial structure and enabled the division into the core necrotic area, intermediate quiescent area, and outer proliferative area from the spatial lipidic ion spectra. Furthermore, we spatially screened and identified 35, 40, and 30 distinct lipid molecules associated with 5-FU treatment in the 3D HepG2 spheroid of necrotic, quiescent, and proliferative areas, respectively. The main dysregulated metabolic pathway was the biosynthesis of glycerophospholipid metabolism, providing the reliable evidence for the mechanism of 5-FU anticancer effect on hepatocellular carcinoma (HCC). In addition, the spatial lipidomic results demonstrated that PA, PC, PG, PI, and SM contents in 5-FU treated tumor spheroids were significantly changed and limited the formation of the central necrotic area. This innovative combination of MALDI MSI with 3D HepG2 spheroids can be conducive to our better understanding of the biological processes between 5-FU triggered lipid metabolism and HCC heterogeneous structure.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Ang Gao,Yong Li,Yinjing He,Can Zheng,Lidan Liu,Zhike Liu,Dapeng Wang,Shengzhong Frank Liu
The quality of organic–inorganic hybrid perovskite films directly affects the application prospect of perovskite solar cells (PSCs), where organic and inorganic cations are the core elements that affect the quality of the perovskite. The additive strategy has been widely used to passivate cation-related defects in perovskite films. Here, the perovskite precursor solution introduced 5-bromopyridine-3-sulfonic acid (BOH) with a potential all-cation passivation function. The experimental results verified that the N atom on pyridine in the BOH molecular structure passivated the defects in perovskite by binding with undercoordination Pb2+, and the sulfonic acid group inhibited nonradiative recombination through their interactions with FA+ and Pb2+, improving perovskite grain size and crystallinity, and enhancing film quality. Thanks to the all-cationic targeted anchoring effect of BOH, the efficiency of the BOH-treated device upgraded from 22.32 to 24.33%. Importantly, PSCs with BOH showed excellent stability after exposure to 25% humidity for 1200 h at room temperature.
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SOLID STATE IONICS
·
2024
·
IF 3
Qin Zhou,Cong Xia,Ji Li,Amei Cheng,Jianwen Liu,Hongying Liu,Lin Li,Shiquan Wang
Metal-organic framework (MOF-5) material was synthesized by solvothermal method. Porous carbon (C) and sulfur-doped porous carbon (S/C) were fabricated by calcining and vulcanization of MOF-5. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and Raman spectroscopy were used to characterize the crystal structure and morphology of the samples. The electrochemical performance of C and S/C materials were tested as anode materials for lithium ion batteries (LIBs) and sodium ion batteries (SIBs). The S/C exhibits the high reversible capacity (1114.8 mAh g −1 after 100 cycles at 0.1 A g −1 and 485 mAh g −1 after 500 cycles at 2.0 A g −1 ), as anode for LIBs. It behaves a reversible discharge capacity of 268.8 mAh g −1 after 100 cycles at 0.1 A g −1 and a capacity of 157 mAh g −1 after 1000 cycles at 2.0 A g −1 , as anode for SIBs. Compared with C, the electrochamical performance of S/C material is significantly improved. This is mainly because the bigger specific surface area and the doping of sulfur atoms increases the layer spacing and facilitates the kinetics of the intercalation and deintercalation of Li + and Na + ions, especially Na + ions.
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Food Bioscience
·
2024
·
IF 4.8
Yilun Huang,Yuwei Liu,Wenting Liu,Huiyu Hu,Daoxue Zhou,Xiaoxiang Peng,Chang Li,Yi Chen,Qiang Yu,Yuting Wang
5 Hydroxymethylfurfural (5-HMF) is a heat-induced hazard that common in baked goods. Although bioactive compounds such as catechins and polysaccharides could inhibit 5-HMF, their inhibition efficiency was limited when used alone. Therefore, this study aimed to explore the synergistic inhibitory impact of catechins and polysaccharides on 5-HMF in biscuits. Epigallocatechin gallate (EGCG), chitosan, and sodium alginate were screened out due to their high maximum inhibition rates (55.90, 67.20 and 66.62%, respectively), and their synergistic inhibitory effects on 5-HMF were investigated by isobologram analysis. The interaction index (γ) varies inversely with the synergism. When γ < 1 or >1, it represented synergism or antagonism, respectively. The results showed the EGCG/chitosan ratios of 1:1 (γ = 0.221) had better synergism than that of 1:2 (γ = 0.671) and 1:3 (γ = 0.523). Moreover, EGCG and sodium alginate only showed a synergistic effect at ratio of 1:1 (γ = 0.358), while an antagonistic effect was observed at ratios of 1:2 and 1:3 (γ = 1.12 and 1.25, respectively). The optimal inhibitory effect (54.57%) was achieved when EGCG-sodium alginate (1:1) at 0.2 g/kg. Compared with those used alone, the co-addition of EGCG and chitosan could decrease the dosage of EGCG to 1/5 and that of chitosan to 1/7, indicating a significant improvement of inhibit efficiency. Finally, Inhibitors’ concentration correlated negatively with 5-HMF content and browning index, suggesting they might inhibit 5-HMF by hindering Maillard or caramelization reactions. This study provided an efficient and cost-effective solution for 5-HMF control in baking products.
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DYES AND PIGMENTS
·
2025
·
IF 4.2
Mengrong Hu,Zheng Li,Shufang Gao,Han Pan,Jian Luo
The excited-state intramolecular proton transfer (ESPT intra ) process in flavonoids has attracted considerable attention due to its fundamental role in their photophysics. This study systematically investigates the photophysical properties of 5-methoxyflavone (5MF), a flavonoid derivative incapable of ESPT intra . It was demonstrated that intermolecular hydrogen bonding with protic solvents effectively suppress the non-radiative ππ∗-nπ∗ internal conversion in 5MF, leading to significant fluorescence enhancement. In highly acidic aqueous solutions (pH < 3.3), an excited-state intermolecular proton transfer (ESPT inter ) process takes place, generating protonated 5MF (5MFH + ) and resulting in fluorescence quenching. Notably, when incorporated in acidic poly(vinyl alcohol) (PVA) films, the excited-state protonated species 5MFH + exhibits intense fluorescence with a long fluorescence lifetime of 8.4 ns, owing to the suppression of non-radiative decay pathways by the rigid polymer network. Additionally, the dense hydroxyl groups in the PVA matrix also enhance the emission of neutral 5MF. These results highlight the potential of both 5MF and 5MFH + as efficient luminescent chromophores for the design of optical materials based on hydrogen-bonding polymer systems.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Yan Wang,Bo Ding,Qingqing Bao,Yunying Zhao,Guohui Li,Yu Deng
Agmatine is an important basic drug and nutritional supplements, which exhibits significant physiological activities. Traditional methods, such as whole-cell catalysis, require substantial pyridoxal 5′-phosphate (PLP) addition, which accounts for 15 % of production costs. In this study, we utilized purified arginine decarboxylase (AdiA-M2) that leverages the intrinsic PLP cofactor, without the need for additional PLP supplementation, and ZIF-8 was employed to enhance the pH tolerance. The successful incorporation of AdiA-M2 into the ZIF-8 structure was confirmed through scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The immobilized arginine decarboxylase demonstrated markedly enhanced performance under alkaline conditions, along with improved thermal stability and storage activity compared to its free enzyme counterpart. Specifically, the immobilized AdiA@ZIF-8 system achieved an initial conversion rate of 95.16 %, after five recycles the titer attained 266.69 g/L without the extra PLP addition, which is 1.67 times higher than the best current production approach. The adopted enzyme-catalysis system eliminated the need for additional cofactors, thereby lowering costs and providing valuable insights for the industrial production of agmatine.
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NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Yongzhao Zhang,Xuzheng Chen,Yunlei Zhou,Tao Feng,Luping Lyu,Zhenya Zhu,Yifan Liu
The efficient transformation of glucose into 5-hydroxymethylfurfural (5-HMF) is crucial for sustainable bio-refinery processes, but achieving high production efficiency remains challenging. In this study, we presented the preparation of Al-containing metal-organic frameworks (MOFs), specifically MIL-53(Al), MIL-53(Al)-NO2, and MIL-53(Al)-NH2, using different ligands as catalysts to enhance the production of 5-HMF from glucose. Comprehensive characterization provided valuable insights into the properties of the synthesized MOFs. The synthesized MOFs exhibited varied MIL-53(Al) states, morphologies, and thermal stabilities based on the different ligands employed, which in turn affected their catalytic performance. Among the Al-containing MOFs synthesized, MIL-53(Al)-NH2 demonstrated remarkable catalytic capabilities for the conversion of glucose to 5-HMF. This MOF achieved an impressive 5-HMF yield of 53% and a reaction selectivity of 54%. The catalytic efficiency was further enhanced by leveraging the synergistic effect of MIL-53(Al)-NH2 in combination with Amberlyst-36, resulting in a 5-HMF yield of 59% and a reaction selectivity of 60%.
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ADVANCED SYNTHESIS & CATALYSIS
·
2025
·Vol. 367
·期 17
·e70093
·
IF 4
Anirban Chowdhury,Akkammagari Putta Rangappa,Wenhua Xue,Yixuan Liu,Jun Zhao
The photocatalytic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-diformylfuran (DFF) is a promising process with significant implications for sustainable energy and environmental applications. However, achieving highly selective DFF production from HMF remains a challenging task, particularly due to the dependency of molecular O 2 activation on the composition and exposed facets of nanomaterials. This study investigates the impact of different preferential facets of hydrothermally synthesized cadmium sulfide (CdS) morphologies—including hollow spheres, nanorods, and flower-like structures—on the photocatalytic oxidation of HMF to DFF. The findings reveal that the preferential CdS (002) facet and Cd vacancies in the hollow sphere morphology significantly enhance photocatalytic activity, achieving 68% HMF conversion and 89% selectivity for DFF production within 12 h of reaction time. This work highlights the critical role of material composition and facet orientation in optimizing photocatalytic processes for selective oxidation reactions, offering a potential strategy for the design of advanced photocatalysts in sustainable chemical production.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Yixuan Wang,Shijia Fu,Yi Lu,Rongrong Lai,Ziyi Liu,Weixuan Luo,Yuehong Xu
Psoriasis does not respond adequately to the monotherapy, tailoring combined strategies for synergistical treatment remains challenging. We fabricated chitosan/hyaluronan nanogels to co-load methotrexate (MTX) and 5-aminoleavulinic acid (ALA), i.e., MTX-ALA NGs, for a combined chemo-photodynamic therapy for psoriasis. Compared with MTX-ALA suspension, the NGs enhanced the penetration and retention of MTX and ALA through and into the skin in vitro and in vivo ( p < 0.001). NGs enhanced the cellular uptake ( p < 0.001), protoporphyrin IX conversion ( p < 0.001), and reactive oxygen species generation (3.93-fold), subsequently exerted the synergistical anti-proliferation and apoptosis on lipopolysaccharide-irritated HaCaT cells with the apoptosis rate of 78.6%. MTX-ALA NGs efficiently ameliorated the skin manifestations and down-regulated the proinflammatory cytokines of TNF-α and IL-17A in imiquimod-induced psoriatic mice ( p < 0.001). Importantly, MTX-ALA NGs reduced the toxicities of oral MTX to the liver and kidney. The results support that MTX-ALA NG is a convenient, effective, and safe combined chemo-photodynamic strategy for psoriasis treatment.
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高影响因子
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