BIOMACROMOLECULES
·
2010
·
IF 4.502
Peng-Fei Gou,Wei-Pu Zhu,Zhi-Quan Shen
Novel drug-conjugated amphiphilic A14B7 miktoarm star copolymers composed of 14 poly(ε-caprolactone) (PCL) arms and 7 poly(ethylene glycol) (PEG) arms with β-cyclodextrin (β-CD) as core moiety were synthesized by the combination of controlled ring-opening polymerization (CROP) and “click” chemistry. 1H NMR, FT-IR, and SEC-MALLS analyses confirmed the well-defined A14B7 miktoarm star architecture. These amphiphilic miktoarm star copolymers could self-assemble into multimorphological aggregates in aqueous solution, which were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Moreover, the drug-loading efficiency and drug-encapsulation efficiency of the drug-conjugated miktoarm star copolymers were higher than those of the corresponding non-drug-conjugated miktoarm star copolymers.
✓ 已验证
DOI
PubMed
高影响因子
Polymer Chemistry
·
2010
·Vol. 1
·期 8
·1205-1214
·
IF 0
Peng-Fei Gou,Wei-Pu Zhu,Zhi-Quan Shen
Novel well-defined drug-grafted seven-arm amphiphilic star poly(ε-caprolactone-co-carbonate)-b-poly(ethylene glycol)s based on a β-CD core [CDS-P(CL-co-DTC)-D-b-PEG] have been synthesized by the combination of controlled ring-opening polymerization (CROP), esterification coupling reactions and “click” reactions. First, 5,5-dibromomethyl-trimethylene carbonate (DBTC) bearing two bromide pendent groups was synthesized and used to copolymerize with CL to prepare seven-arm star random copolymers [CDS-P(CL-co-DBTC)] in the presence of per-2,3-acetyl-β-cyclodextrin and Sn(Oct)2. Second, esterification coupling reaction between CDS-P(CL-co-DBTC) and carboxyl-terminated mPEG led to amphiphilic seven-arm star copolymers [CDS-P(CL-co-DBTC)-b-PEG]. Subsequently, the bromide pendent groups on CDS-P(CL-co-DBTC)-b-PEG had been converted into azide groups by treating with NaN3. Finally, alkyne functionalized ibuprofen had been grafted onto the hydrophobic block of the star copolymers by copper(I)-catalyzed “click” reaction. 1H NMR, FT-IR and SEC analyses confirmed the well-defined drug grafted star architecture. These copolymers could self-assemble into multi-morphological aggregates in water, which were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
✓ 已验证
产品关联
DOI
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2011
·
IF 2.971
Wei Liu,Jurui Qi,Linna Yan,Qiong Jia,Cong Yu
This paper presents a study of the synthesis of a polymer monolith column and its application to the analysis of PAHs in smoked meat products. A poly(butyl methacrylate-co-ethylene glycol dimethacrylate) monolith capillary has been successfully prepared with in situ polymerization method. The polymer monolith microextraction combined with HPLC determinations is employed for the analysis of naphthalene , biphenyl , phenanthrene , and anthracene . Various parameters affecting the extraction efficiency have been investigated and optimized. Under the optimum experimental conditions, the method provides an acceptable linearity (2–10,000 μg/L), low limits of detection (1.4–2.0 μg/L), and good precision (intraday relative standard deviations < 4.1%, interday relative standard deviations < 5.7%). When applied to the determination of the four PAHs in smoked meat samples, recoveries are obtained in the range of 86.6–101.5%.
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2026
·
IF 1.8
Xin Zhang,Jingcheng Huang,Rui Xu,Jiashuai Sun,Pengwei Zhang,Xingkang Zhang,Fangling Du,Yemin Guo,Xia Sun
There is a growing concern that residues of agricultural chemicals in fresh produce may pose a risk to public health. Acetamiprid, a broad spectrum insecticide, has attracted increasing attention due to its potential toxicity and environmental persistence. Although conventional analytical techniques offer high accuracy, they are often labor intensive and instrument dependent. To address these limitations, this study aimed to develop a rapid, sensitive, and straightforward method for the detection of acetamiprid residues in leafy vegetables. This study was the first to utilize the water retention property of hydrogels in the detection reaction. The assay could be conducted directly on the vegetable surface without spraying detection reagents. This design greatly simplified the analytical procedure. A fluorescent aptamer based composite hydrogel sensor was successfully fabricated using polyvinyl alcohol (PVA), a polyethylene glycol (PEG) based crosslinked polymer, and aptamer functionalized graphene quantum dots (GQDs-Apt). The composite hydrogel was crosslinked with glutaraldehyde (GA), resulting in a stable three-dimensional network structure with excellent biocompatibility and mechanical strength. The GQDs-Apt conjugates served as the signal reporting units, whose fluorescence characteristics were modulated by the binding interaction between the aptamer and acetamiprid, thereby conferring high sensitivity and selectivity. In the absence of acetamiprid, the aptamer probe remained associated with the graphene quantum dot surface, leading to fluorescence quenching. Upon introduction of the target pesticide, the aptamer preferentially bound to acetamiprid, inducing a conformational change that facilitated detachment of the probe from the quantum dot surface and subsequent fluorescence recovery. This "quenching–recovery" signaling mechanism provided the theoretical basis and practical framework for the quantitative detection of acetamiprid. The fluorescence changes within the hydrogel network were exploited for both the screening and precise quantification of the pesticide. Performance metrics revealed a limit of detection (LOD) of 0.005 μmol L−1, a dynamic range of 0.01 to 10 μmol L−1, and R2 of 0.9971. Validation in real matrices of cabbage, leek and tomato demonstrated robust accuracy and repeatability, with fortified recoveries between 96.11% and 99.47%, relative standard deviation (RSD) below 3.11%, and an equilibration time of less than 20 minutes. This strategy provides a rapid, user friendly, high resolution, and reliable tool for agricultural product safety and environmental monitoring.
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 4
·e70406
·
IF 3.6
Shuang Feng,Hongjie Ju,Yingxia Guo,Yue Deng,Jiarui Zhang,Jiale Liu,Xinyue Zhou,Hongyu Xue,Meiyun Shi,Lei Yin
Here we present a novel, green analytical platform for the sensitive quantification of the short‑chain excipient monomethoxy polyethylene glycol amine, based on ultra-high-performance supercritical fluid chromatography-tandem mass spectrometry. The core innovation is the effective coupling of supercritical carbon dioxide chromatography with the selective monitoring of protonated molecular ions ([M+H] + ), which collectively enhance sensitivity and minimize matrix interference. This method enables exceptionally rapid analysis, achieving baseline separation within 3.4 min, and demonstrates high sensitivity with a lower limit of quantification of 5 ng/mL in rat plasma. Comprehensive validation confirmed its robustness, precision, and accuracy across the analytical range. Its environmental sustainability and practical utility were quantitatively endorsed by excellent Analytical GREEnness (0.66) and Blue Applicability Grade Index (72.5) scores. As a demonstrative application, the validated strategy was successfully employed in a rat pharmacokinetic study, establishing a fast, green, and reliable analytical framework for the safety evaluation of PEGylated compounds.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2026
·
IF 3.4
Shuang Feng,Yue Deng,Jiarui Zhang,Jiale Liu,Xinyue Zhou,Yalin Xi,Lei Yin,Meiyun Shi
Short-chain polyethylene glycol derivatives, such as methoxy polyethylene glycol with 6 subunits (M‑PEG₆‑OH), are extensively utilized excipients and building blocks, yet their in vivo biodistribution and associated safety profiles remain insufficiently characterized. To address this knowledge gap, this study developed a novel, environmentally conscious analytical platform based on ultra‑performance convergence chromatography‑tandem mass spectrometry (UPC²‑MS³) for the sensitive quantification of M‑PEG₆‑OH in zebrafish tissues. A key methodological innovation involved the selective formation and detection of ammonium adducts ([M+NH₄]⁺) as precursor ions, coupled with a post‑column infusion strategy. This combined approach effectively mitigated ion suppression and significantly enhanced ionization efficiency, yielding an average 5‑fold increase in analytical signal response. The fully validated method achieved rapid and baseline separation of the analyte within 1.5 min. Its successful application to a tissue distribution study revealed distinct, time‑dependent accumulation profiles, with the highest levels observed in gill, followed by liver and muscle tissues. Furthermore, the methodology was rigorously evaluated using established metric systems, demonstrating excellent environmental sustainability (AGREE score = 0.62) and outstanding practical applicability (BAGI score = 72.5). This work provides crucial insights into the tissue‑specific disposition of short‑chain PEG derivatives and establishes a sustainable, high‑performance analytical framework for the safety evaluation of PEGylated compounds.
✓ 已验证
DOI
PubMed
高影响因子
Polymers
·
2020
·Vol. 12
·期 5
·1181
·
IF 3.426
Lan Yao,Haihui Li,Kai Tu,Lifen Zhang,Zhenping Cheng,Xiulin Zhu
Polymeric nanomaterials made from amphiphilic block copolymers are increasingly used in the treatment of tumor tissues. In this work, we firstly synthesized the amphiphilic block copolymer PBnMA-b-P(BAPMA-co-PEGMA) via reversible addition-fragmentation chain transfer (RAFT) polymerization using benzyl methacrylate (BnMA), poly (ethylene glycol) methyl ether methacrylate (PEGMA), and 3-((tert-butoxycarbonyl)amino)propyl methacrylate (BAPMA) as the monomers. Subsequently, PBnMA-b-P(APMA-co-PEGMA)@NIR 800 with photothermal conversion property was obtained by deprotection of thetert-butoxycarbonyl (BOC) groups of PBAPMA chains with trifluoroacetic acid (TFA) and post-modification with carboxyl functionalized ketocyanine dye (NIR 800), and it could self-assemble into micelles in CH3OH/water mixed solvent. The NIR photothermal conversion property of the post-modified micelles were investigated. Under irradiation with NIR light (λmax= 810 nm, 0.028 W/cm2) for 1 h, the temperature of the modified micelles aqueous solution increased to 53 °C from 20 °C, which showed the excellent NIR photothermal conversion property.Keywords:amphiphilic block copolymer;self-assembly;NIR photothermal conversion;reversible addition-fragmentation chain transfer (RAFT) polymerization
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 34
·17768-17779
·
IF 3.708
Yinwen Li,Huilong Guo,Yunfei Zhang,Jian Zheng,Jianqun Gan,Xiaoxiao Guan,Mangeng Lu
A series of well-defined adamantyl-terminated thermally responsive copolymers (Ad-POEGMAs) were synthesized by atom transfer radical polymerization (ATRP), in which 2-(2-methoxyethoxy) ethyl methacrylate (MEO2MA) and oligo(ethylene glycol) methacrylate (OEGMA) served as the thermosensitive building blocks. Moreover, cyclodextrins (CDs) as bulky pendant grafted polymers (PGCD) were synthesized by homopolymerization of aminoethyl methacrylate β-cyclodextrin (GCD). The thermal-responsive behaviors were investigated by a combination of 1H NMR, UV-vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). In comparison to other thermal-responsive copolymers based on POEGMAs, Ad-POEGMAs exhibited unusual thermally induced aggregation processes. The Ad group assembled and POEGMA chains associated to produce stable water-soluble nano-aggregates, followed by a rearrangement process at the second thermal transition. Moreover, it was found that a noncovalently connected supramolecular pseudo-graft polymer was formed via inclusion complexation in aqueous solution. This pseudo-graft polymer underwent a reversible temperature-induced transition from solution to micelle under suitable conditions. The cyclodextrin (CD) moiety attached to the main chain played two roles. As supramolecular host moieties, CDs formed inclusion complexes with guest-ended polymers, leading to graft-like polymers. As bulky hydrophilic moieties, CDs stabilized the micelles induced by the coil-to-globule transition of POEGMA segments.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Jie Yang,Li-Sheng Tang,Rui-Ying Bao,Lu Bai,Zheng-Ying Liu,Wei Yang,Bang-Hu Xie,Ming-Bo Yang
Phase change materials (PCMs) with high thermal conductivity and efficient solar energy conversion have recently attracted much attention. However, a facile strategy to enhance thermal conductivity and realize energy conversion and storage is still eagerly desired. In this work, a very low content of graphene nanoplatelets (GNP) is introduced into poly (ethylene glycol) (PEG)/boron nitride (BN) composite PCMs, resulting in great improvement in thermal conductivity and photoabsorption ability via a facile solution blending process. The presence of GNP contributes to enhancing thermal conductivity and realizing efficient solar energy conversion (including solar-to-thermal and solar-to-electric energy conversion) for the PEG/BN/GNP composite PCMs owing to the formation of improved BN/GNP thermally conductive network and the improvement of sunlight harvesting ability, respectively. Thermophysical properties demonstrate that compared with PEG/BN composite PCMs at the same BN content, PEG/BN/GNP composite PCMs containing a low GNP content maintain comparable energy storage density. The study sheds light on the realization of solar energy utilization and storage of the organic PCMs requiring high thermal conductivity.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Fang Shan,YuJuan Liu,Haiying Jiang,Fei Tong
Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly( l -lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1 H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Xinran Cui,Yongbin Wang,Yan Zhan,Xiaoyan Chen
Dimethoxyethyl phthalate (DMEP) is an industrial phthalates reagent exposed to human body. Its hydrolytic metabolite ethylene glycol methyl ether (EGME) and further oxidative metabolite methoxyacetic acid (MAA) have reproductive and developmental toxicity, and the latter is more toxic than the former. However, due to the lack of sensitive and reliable analytical method, the in vivo clearance and biotransformation of EGME and MAA is poorly understood. In order to evaluate their toxicokinetic profiles, a precolumn derivatization liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of EGME and MAA in rat plasma. After a simple protein precipitation procedure, the sample was derivatized with p -toluene sulfonyl isocyanate (PTSI) and 4-bromo- N -methylbenzylamine (4-BNMA), separated by gradient elution on HSS T3 column, and detected by electrospray ionization (ESI) source in positive multiple reaction monitoring (MRM) mode. The results of the method validation showed that EGME had good linearity in the range of 2.00–400 ng/mL and MAA in the range of 5.00–1000 ng/mL. The intra- and inter-day accuracy and precision were within acceptable limits ( ± 15 %) at all concentrations. This validated method was successfully applied to characterize the toxicokinetics of EGME and MAA in rats following a gavage administration of 50 mg/kg DMEP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2014
·Vol. 10
·期 6
·948-958
·
IF 7.578
Liu Yongjun,Liu Chunxi,Li Min,Liu Fengxi,Feng Lixia,Zhang Li,Zhang Na
Multifunctional nanoparticles assembled from multi-block polymers are now one of the most convenient and convincing carriers for target drug delivery. Multi-block polymers could provide multi-functions such as sufficient drug loading capability and efficient target ligand coupling potency.
In this article, novel multi-block polymer poly(lactic acid)–poly(ethylene glycol)–poly(L-lysine) (PLA-PEG-PLL) with relatively precise block molecular weight were synthesized by new method which we called polymer–polymer conjugation. This method conjugated different polymer
blocks by reactions between the terminal active groups of different blocks, thus simplified the synthesis procedure. The obtained PLA-PEG-PLL was characterized by 1H NMR and gel permeation chromatography. The controlled drug delivery capability and the target ligand coupling potency
of PLA-PEG-PLL were verified using paclitaxel (PTX) as model drug and vascular endothelial growth factor (VEGF) antibody as target ligand. The PTX-loaded PLA-PEG-PLL nanoparticles (PNP) and VEGF antibody modified PTX-loaded PLA-PEG-PLL nanoparticles (VPNP) were prepared using solvent diffusion
methods. The two nanoparticles showed spherical or ellipsoidal shapes with uniform particle size distribution (190.1 ± 1.27 nm and 203.6 ± 4.10 nm for PNP and VPNP, respectively) and positive zeta potential (23.76 ± 0.72 mv and 20.76 ± 0.34 mv for PNP and VPNP,
respectively). The cellular cytotoxicity, cellular uptake, in vivo therapeutic effects of the two nanoparticles were investigated. Cytotoxicity of VPNP against HepG2 cells was superior to that of PNP and Taxol®. The VPNP and PNP showed better antitumor efficacy in a murine model
bearing H22 compared with Taxol® and VPNP was the best. The study on cellular uptake indicated that the better antitumor efficacy of VPNP was attributed to the increased uptake of drug by tumor cells. These results demonstrated that PLA-PEG-PLL was a favorable multifunctional
material for drug target delivery and polymer–polymer conjugation was a promising method to fabricate novel multi-block polymers.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）