DIGESTIVE DISEASES AND SCIENCES
·
2023
·
IF 3.1
Zhao Lianrong,Wu Di,Qu Qiao,Li Zhilong,Yin Hongzhuan
Background/Aims Aberrant nuclear factor-κB p65 (NF-κB p65) nuclear import commonly occurs in multiple cancers, including colon cancer. According to BioGRID, we noted that Karyopherin subunit alpha 1 (KPNA1), an important molecular transporter between the nucleus and the cytoplasm, may interact with NF-κB p65. KPNA1 itself is highly expressed in colon adenocarcinoma samples ( N = 286) based on The Cancer Genome Atlas (TCGA) database. We aimed to explore the role of KPNA1 in colonic carcinogenesis and to determine whether NF-κB p65 nuclear translocation was involved. Methods KPNA1 expressions at mRNA and protein levels were analyzed in colon cancer tissues. The regulatory effect of KPNA1 on malignant biological properties was detected in SW480 and HCT116 colon cancer cells. Coimmunoprecipitation and immunofluorescence were performed to verify the relationship between KPNA1 and NF-κB p65. KPNA1 ubiquitination was also preliminarily investigated. Results KPNA1 was firstly confirmed as a significantly upregulated gene in our collected clinical colon cancer samples ( N = 35). KPNA1 depletion inhibited cell proliferation, induced cell cycle arrest, and diminished migratory and invasive capacity of SW480 and HCT116 cells. Colon cancer cells overexpressing KPNA1 acquired more aggressive behaviors. KPNA1 acted as a transporter to induce the nuclear accumulation of NF-κB p65, thereby activating NF-κB signaling pathway in colon cancer cells. Furthermore, HECT, C2, and WW Domain-Containing E3 Ubiquitin (HECW2) interacted with KPNA1 to induce its ubiquitination. KPNA1 labeled with polyubiquitins was degraded through ubiquitin–proteasome system. Conclusion The present study uncovers a role of KPNA1-NF-κB p65 axis in promoting colonic carcinogenesis. Graphical Abstract
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高影响因子
Science China-Chemistry
·
2023
·Vol. 66
·期 5
·1384-1393
·
IF 9.6
Sun Yi-Fan,Liu Ya-Jie,Wang San-Tai,Liu Xiao-Yu,Ma Chao,Fang Wei-Hui,Zhang Jian
Based on our discovery of neutral aluminum molecular rings, we herein introduce rare earth ions into the center of the ring and reveal their structural adaptability. The resulting yoyo-like cationic macrocycles are highly adaptive to guests, creating unusual supramolecular assemblies. The first category is stacked with singly oriented rhombohedral channels, which exhibit fast and reversible solvent-triggered molecular motions and structural rearrangements The second type of assembly is that yoyo macrocycles and guest molecules form a nested host-guest sodalite cage supramolecular structure. It is uncommon for guest molecules to be well-defined within cages although caged structures have been well-documented Their host-guest interaction discussion reveals the self-adaptation and flexibility of the yoyo macrocycles. Considering their good water stability, we investigated their anion exchange properties. The results show that they have significant exchange capacity for KI/I 2 , \({\rm{Re}}{{\rm{O}}_4}^ - \) , and \({\rm{Mn}}{{\rm{O}}_4}^ - \) , revealing their potential application in water purification and nuclear waste treatment.
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CANCER GENE THERAPY
·
2023
·
IF 6.4
Ma Jia,Zhou Wenyang,Yuan Yifeng,Wang Baosheng,Meng Xiangpeng
Proteasome 26S subunit, non-ATPase 12 (PSMD12) genes have been implicated in several types of malignancies but the role of PSMD12 in pancreatic cancer (PC) remains elusive. Bioinformatics analysis showed that PSMD12 was highly expressed in PC patients and was associated with shorter overall survival. PSMD12 was also shown to be highly expressed in PC tissues and cell lines. Upregulated PSMD12 showed enhanced cell viability, increased colony formation rate and upregulated levels of PCNA and c-Myc, while the inhibition of PSMD12 abated these levels. PSMD12 knockdown promoted cell apoptosis. The results of xenografts in nude mice confirmed that PSMD12 promoted PC tumor growth in vivo. Protein ‒ protein interaction network and functional enrichment analyses implied that PSMD12 may have a connection with cyclin-dependent kinase inhibitor 3 (CDKN3). Co‑immunoprecipitation and western blot results confirmed that PSMD12 could interact with and abate the ubiquitination level of CDKN3, thus stabilizing the CDKN3 protein. Rescue assays showed that PSMD12 overexpression caused cell proliferation and that knockdown-induced cell apoptosis could be reversed by CDKN3 regulation. This work reveals the essential roles of PSMD12 in the proliferation and apoptosis of PC development. PSMD12 may regulate CDKN3 expression by interacting with and abating the ubiquitination level of CDKN3, thereby participating in the malignant behavior of PC.
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CATALYSIS LETTERS
·
2023
·
IF 2.8
Zhao Liping,Wang Changzhen,Bai Juan,Wu Ruifang,Li Haitao,Zhao Yongxiang
2-Hydroxytetrahydrofuran (HTHF) is the main by-product of hydrogenation of 1,4-butynediol (BYD) to 1,4-butanediol (BDO) in industry, which is difficult to be effectively removed in the subsequent distillation process and seriously affects the product quality. Here, a series of silica-supported bimetallic Ni–Fe catalysts with different Fe/Ni weight ratios were prepared by using incipient wetness impregnation method and investigated the effect of Fe on the catalyst performance in the hydrogenation of HTHF to BDO. In comparison with monometallic Ni and Fe catalysts, bimetallic Ni–Fe catalysts exhibited better performance for HTHF hydrogenation due to the formation of Ni–Fe alloy phase identified by XRD, TEM, STEM-EDS, H 2 -TPR, NH 3 /H 2 -TPD, Pyridine FTIR and XPS, etc. A mechanism is proposed to explain the promoting effect of Fe, which can be assigned to the synergism between nickel sites with high ability to activate/overflow hydrogen and Fe-containing sites with strong oxophilicity for the activation of the O-C-O bond over the tetrahydrofuran ring. Graphical Abstract
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-11
·
IF 5.7
Li Yangguang,Yang Qiaoran,Feng Yifan,Ye Bang-Ce
For practical analysis and simultaneous detection of arbutin (AR) and hydrochinone (HQ) in cosmetics, an electrochemical sensor has been designed based on nitrogen and sulfur co-doped Fe–Ni alloy (N,S-FeNi 3 /C) nanoparticles. The N,S-FeNi 3 /C has been prepared for the first time via hydrothermal synthesis and high-temperature carbonization. N,S-FeNi 3 /C not only improves the charge transfer to the surface, but also provides rich active sites and fast ion diffusion rates owing to the iron and nickel bimetallic materials. In addition, the d-band structure of transition metals (nickel and iron) introduced by the N and S atoms exhibits an electronic structure similar to that of noble metal catalysts, thus enhancing electrocatalytic activity and increasing conductivity. Additionally, the double doping of S and N atoms significantly increases the active sites of carbon atoms; thus, N-S-FeNi3/C exhibits excellent electrochemical catalytic activity for the oxidation of AR and HQ. Further, the N,S-FeNi 3 /C sensor is used for the simultaneous determination of HQ and AR by square-wave pulse voltammetry. Distinct oxidation peaks of HQ and AR are observed at potentials of +0.028 V and +0.352 V (vs. SCE). The electrical signal increases linearly in the HQ concentration ranges of 0.1–100 μM and 0.05–70 μM for the simultaneous determination of AR and HQ with a detection limit as low as 0.0476 and 0.0135 μM (S/N = 3), respectively. Thus, rapid and accurate detection of AR and HQ in spiked cosmetics is successfully achieved, with a recovery ranging from 96.4 to 104.2%, and the relative standard deviation is lower than 3.8–4.0%. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 14
·2677-2692
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IF 4.3
Meng Xin,Zong Hou,Zheng Zhong,Xing Junpeng,Liu Zhiqiang,Song Fengrui,Liu Shu
α-Glucosidase inhibitors in natural products are one of the promising drugs for the treatment of type 2 diabetes. However, due to the complexity of the matrix, it is challenging to comprehensibly clarify the specific pharmacodynamic substances. In this study, a novel high-throughput inhibitor screening strategy was established based on covalent binding of α-glucosidase on chitosan-functionalized multi-walled carbon nanotubes coupled with high-resolution mass spectrometry. The synthesized MWCNTs@CS@GA@α-Glu was characterized by TEM, SEM, FTIR, Raman, and TG. Performance studies showed that the microreactor exhibited stronger thermostability and pH tolerance than that of the free one while maintaining its inherent catalytic activity. Feasibility study applying a model mixture of known α-glucosidase ligand and non-ligands indicated the selectivity and specificity of the system. By integrating ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-QTOF-MS) with ion mobility mass spectrometry (IMS), 15 ligands were obtained and tentatively identified from Tribulus terrestris L., including 8 steroidal saponins, 4 flavonoids, and 3 alkaloids. These inhibitors were further validated by in vivo experiments and molecular docking simulation.
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Advanced Composites and Hybrid Materials
·
2023
·Vol. 6
·期 2
·1-13
·
IF 20.1
Lian Peng,Yan Ruihan,Wu Zhiguo,Wang Zhibin,Chen Ying,Zhang Li,Sheng Xinxin
Inorganic hydrated salt phase change materials (PCMs) have received great attention due to their capabilities to reduce building energy consumption and improve building thermal comfort. In this work, a modified PCM (DHPD-STP) with a low supercooling degree was first prepared by using disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 ·12H 2 O, DHPD) as the matrix and sodium thiosulfate pentahydrate (Na 2 S 2 O 3 ·5H 2 O, STP) as a nucleating agent. Then expanded graphite (EG) was added to DHPD-STP to afford a novel form-stable composite phase change material (CPCM). The novel CPCM obtained had a phase transition temperature (34.74 °C) appropriate for building thermal management, an excellent phase change enthalpy (184.39 J/g), and an extremely low supercooling degree (1.1 °C). The addition of EG greatly improved the form stability and cycle stability of the CPCM. Even after 200 cycles, the CPCM prepared still had a high enthalpy of phase changes (164.54 J/g). More importantly, the use of CPCM-loaded roofs effectively mitigated the variation of room temperature and improved indoor thermal comfort. The above results demonstrate the great potential of the CPCM prepared for passive building thermal management. Graphical Abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-11
·
IF 16.6
Liang Guojin,Liang Bochun,Chen Ao,Zhu Jiaxiong,Li Qing,Huang Zhaodong,Li Xinliang,Wang Ying,Wang Xiaoqi,Xiong Bo,Jin Xu,Bai Shengchi,Fan Jun,Zhi Chunyi
The chlorine-based redox reaction (ClRR) could be exploited to produce secondary high-energy aqueous batteries. However, efficient and reversible ClRR is challenging, and it is affected by parasitic reactions such as Cl 2 gas evolution and electrolyte decomposition. Here, to circumvent these issues, we use iodine as positive electrode active material in a battery system comprising a Zn metal negative electrode and a concentrated (e.g., 30 molal) ZnCl 2 aqueous electrolyte solution. During cell discharge, the iodine at the positive electrode interacts with the chloride ions from the electrolyte to enable interhalogen coordinating chemistry and forming ICl 3 - . In this way, the redox-active halogen atoms allow a reversible three-electrons transfer reaction which, at the lab-scale cell level, translates into an initial specific discharge capacity of 612.5 mAh g I2 −1 at 0.5 A g I2 −1 and 25 °C (corresponding to a calculated specific energy of 905 Wh kg I2 −1 ). We also report the assembly and testing of a Zn | |Cl-I pouch cell prototype demonstrating a discharge capacity retention of about 74% after 300 cycles at 200 mA and 25 °C (final discharge capacity of about 92 mAh).
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Cancer Nanotechnology
·
2023
·Vol. 14
·期 1
·1-19
·
IF 5.7
Wu Yueyao,Wang Runtian,Shen Peiliang,Zhou Weijia,Chen Chao,Yang Kaiyong,Yang Jingjing,Song Yujun,Han Xin,Guan Xiaoxiang
Cancer treatment by inducing tumor cell immunogenic cell death (ICD) is critical for tumor therapy. However, ICD activation by single pathway is often limited in practical application due to its low efficiency. In addition, the low pH and anoxic microenvironments in solid tumors greatly limit the effective activation of ICD. Herein, hollow manganese dioxide (H-MnO2) nanomaterials were selected to load both Mitoxantrone (MTZ) and Chlorin e6 (Ce6) due to its hollow structure and ability to release drugs in the acidic environments. Thus, the synergy of photodynamic therapy (PDT), photothermal therapy (PTT) and chemotherapy can induce the process of immunogenic cell death, stimulate the maturation of dendritic cells (DCs), and activate the immune response to kill tumor cells dramatically. Efficient immunotherapeutic effects were obtained when MnO2-C/M-HA was given intravenously to 4T1 tumor-bearing BALB/c mice with 660 nm near-infrared laser irradiation. This study overcame the limitations of monotherapy and provided a multifunctional platform for tumor immunotherapy.
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JOURNAL OF PEST SCIENCE
·
2023
·
IF 4.8
Nwanade Chuks Fidelis,Wang Min,Yu Zhijun,Liu Jingze
To develop an appropriate program for essential oils (EOs) used in pest control, it is important to acquire information about the effect of the EO when used at a low concentration. Previously, we demonstrated the enzymatic activity of Haemaphysalis longicornis (the main livestock pest in Australia and New Zealand) exposed to Cinnamomum cassia EO and ( E )-cinnamaldehyde (its major compound) when applied at 50% lethal concentration (LC 50 ). However, H . longicornis respond to toxic substances via different mechanisms and at different times of exposure. Herein, the enzymes involved in the defense response were studied by monitoring glutathione-S-transferase, acetylcholinesterase, and Na + /K + -adenosine triphosphatases (ATPase) activities in H . longicornis larvae exposed to C . cassia EO and ( E )-cinnamaldehyde across different times. Further, the molecular changes related to the defense mechanisms were studied by monitoring adenosine triphosphate (ATP)-binding cassette (ABCB10) gene expression. In addition, we investigated the acaricidal activity of the test substances against the engorged larvae and nymphs of H . longicornis . Our results showed that the activities of these enzymes were significantly affected by the EO and/or ( E )-cinnamaldehyde depending on the exposure time. We observed up-regulation of the ABCB10 gene in the larvae. Also, our results revealed that C . cassia EO (LC 50 = 1.46 and 16.69 mg/mL) and ( E )-cinnamaldehyde (LC 50 = 3.86 and 17.96 mg/mL) were effective against the engorged larvae and nymphs, respectively. Our results provided a deeper understanding of the defense mechanisms of H . longicornis, which may provide an important clue for the development of EO-based acaricides that are cheaper and environmentally friendly.
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JOURNAL OF POROUS MATERIALS
·
2023
·Vol. 30
·期 5
·1675-1685
·
IF 2.6
Liu Xiaolin,Zeng Zexin,Ning Xutao,Zeng Bin,He Tao,Li Kewen,Zhang Lei
A facile method for the preparation of oxygen and nitrogen self-doped biochar is developed in this work through the application of one-step chemical activation by using pre-oxidized camellia combing graphene oxide. The extracted results revealed that the obtained biochar had a micropores structure while the nitrogen, oxygen heteroatoms were successfully homogeneously introduced into the carbon frameworks. The specific surface area of the GC-700-4 was about 1343 cm 2 g −1 with oxygen and nitrogen contents of nearly 15.6 at.% and 2.4 at.%, respectively. When used as an electrode for supercapacitors, the GC-700-4 compound shows the highest area-specific capacitance of 2,206 mF cm −2 at 1 mA cm −2 , and capacitance retention of about 97.4% after 5000 cycles. Moreover, the proposed symmetric supercapacitor device was fabricated based on GC-700-4, which exhibits a high gravimetric capacitance value of 236.5 F g −1 and capacity retention of 94.2% after the enforcement of 10,000 cycles at 20 A g −1 , as well as an excellent rate capability. These results confirm that graphene wrapped in biochar can change its morphology, and improve its conductivity and electrochemical performance, while graphene wrapped on the surface of biochar is only beneficial to improve the cycle stability of capacitors.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 11
·4840-4852
·
IF 4.5
Wang Xinyu,Zhang Qinan,Zhu Hongyi,Wu Fang,Qiao Yanxiu,Jia Kaihong,Xiang Yong,Hu Xiaoran
Piezoelectric generators (PEGs) have applications in future wearable and implantable technologies, but suffer from insufficient flexibility, poor biocompatibility, facile biodegradation, and low energy output. In this work, a design concept for a piezoelectric elastomer is proposed. Uniquely, we focus on developing a complete “green” design concept, considering the selection of a bio-based monomer, polymer fabrication, and biodegradation. The piezoelectric elastomer, which was prepared by the copolymerization of lactate, 1,4-butanediol, and sebacate monomers, is capable of generating an output voltage of 303.75 mV cm −2 and an output current of 1.92 × 10 –2 mA cm −2 , which are 7.11 times and 4.01 times, respectively, higher than those of a conventional polyvinylidene fluoride–trifluoroethylene copolymer (PVDF-TrFE)-based PEG under the same measurement conditions. Such efficient piezoelectric conversion is attributed to the reduced elastic modulus of the elastomer, which resulted from the random polymerization between piezoelectric lactate monomers and linear 1,4-butanediol and sebacate monomers. As a result, the elastomer generates a greater piezoelectric charge on deformation than traditional PVDF-TrFE. Further, the relative growth rate of L929 mouse fibroblast cells grown on the modified elastomer was unaffected, demonstrating the good biocompatibility of the piezoelectric elastomer. On the basis of the overall environmentally friendly product cycle and high piezoelectric output, this PEG shows promise for applications in future wearable and implantable technologies. Graphical abstract
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危险品化学品经营许可证（带存储）