Food Bioscience
·
2024
·
IF 4.8
Linwei Zhao,Donghua Wang,Jiaye Yu,Xu Wang,Tong Wang,Dianyu Yu,Walid Elfalleh
In this study, proanthocyanidins (PA) and α-tocopherol (α-TOC) were co-encapsulated in complex phospholipid liposomes, to improve the chemical stability of PA and α-TOC and the antioxidant properties of the liposomes. The results showed that the liposome had a spherical microstructure with no adhesions. The encapsulation efficiency of PA and α-TOC reached 78.02% ± 2.02% and 81.67% ± 1.25%, respectively, and interacted with the phospholipid bilayer through hydrogen bonding. The addition of hydrogenated soybean lecithin (HSL) conferred better stability to the liposomes and improved their protection against PA and α-TOC. The retention of PA and α-TOC in liposomes remained above 60% after 30 d of storage at 4 °C. Due to the synergistic antioxidant effect of PA and α-TOC, the DPPH radical scavenging activity of PA-TOC-CPL reached 87.52%, which was significantly higher than that of single-embedded PA and α-TOC liposomes. The release of PA and α-TOC from PA-TOC-CPL during simulated digestion was low and the release pattern could be described by the Ritger-Peppas model. This serves as a reference to guide the utilization of HSL and the development of liposomal products with higher antioxidant properties.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 41
·期 3
·797-805
·
IF 2.557
Hui Zhang,Xiaojing Zhang,Huijie Jiang,Cong Xu,Shengqiang Tong,Jizhong Yan
Shenqi Jiangtang Granule, a well-known traditional Chinese herbal preparation, has been widely used for the treatment of type II diabetes mellitus. In this work, an ultrafiltration liquid chromatography with quadrupole time-of-flight mass spectrometry method was proposed for the rapid identification of bioactive ingredients from Shenqi Jiangtang Granule using α-glucosidase as an example. First, the chemical profile of this preparation was clarified, including 37 saponins, 17 flavonoids, 37 lignans, and seven other compounds. After incubation with α-glucosidase in vitro, the methanol extract with an IC 50 value of 0.19 mg/mL exhibited significant inhibitory activity. Then, 18 specific binding peaks were screened, and 15 peaks were identified. Among these, ten compounds were reported to have potential α-glucosidase inhibitory activity for the first time. Subsequently, the inhibitory activities of these active compounds were evaluated by ultraviolet spectrophotometry with p -nitrophenyl α -d- glucopyranoside as a substrate. As a result, gomisin J and gomisin D exhibited stronger α-glucosidase inhibitory activities than other active compounds with IC 50 values of 77.69 and 133.85 μM, respectively. The results demonstrated that the integrated ultrafiltration liquid chromatography with mass spectrometry method was an effective and powerful tool for the discovery of active ingredients in Shenqi Jiangtang Granule.
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JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Shuxian Zhang,Xiaoying Wang,Xiaofei Wang,Xiaoxuan Fan,Keshuai Liu,Yuping Sa,Gidion Wilson,Xueqin Ma,Guoning Chen
α-Glucosidase plays a direct role in the metabolic pathways of starch and glycogen, any dysfunction in its activity could result in metabolic disease. Concurrently, this enzyme serves as a target for diverse drugs and inhibitors, contributing to the regulation of glucose metabolism in the human body. Here, an integrated analytical method was established to screen inhibitors of α-glucosidase. This step-by-step screening model was accomplished through the biosensing and affinity chromatography techniques. The newly proposed sensing program had a good linear relationship within the enzyme activity range of 0.25 U mL −1 to 1.25 U mL −1 , which can quickly identify active ingredients in complex samples. Then the potential active ingredients can be captured, separated, and identified by an affinity chromatography model. The combination of the two parts was achieved by an immobilized enzyme technology and a microdevice for reaction, and the combination not only ensured efficiency and accuracy for inhibitor screening but also eliminated the occurrence of false positive results in the past. The emodin, with a notable inhibitory effect on α-glucosidase, was successfully screened from five traditional Chinese medicines using this method. The molecular docking results also demonstrated that emodin was well embedded into the active pocket of α-glucosidase. In summary, the strategy provided an efficient method for developing new enzyme inhibitors from natural products.
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CATALYSIS COMMUNICATIONS
·
2024
·
IF 3.4
Chenan Yang,Yi Liu,Ying Hu,Peng Wang,Yuling Yang,Shangxing Chen,Zongde Wang,Xiang Li
Catalytic valorization of biomass into high energy density fuels plays a vital role in order to achieve future carbon neutrality. Herein, a class of H-MZ-5 catalysts with different mesoporous pore structures and acid site properties were synthesized. Among them, HMZ-5 prepared with tetrapropylammonium bromide (TPABr) and hexadecyltrimethylammonium bromide (CTAB) as templating agents showed the most excellent catalytic activity and selectivity of α-pinene dimerization. Under optimal catalytic conditions, 100% α-pinene was converted with 89.6% selectivity to dimers. The efficient catalytic activity and affordability of the catalysts in this system make it promising for industrial applications.
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ADVANCED POWDER TECHNOLOGY
·
2024
·
IF 4.2
Zunlan Hu,Fangnan Zhao,Zhipeng Xie
A facile procedure was provided to obtain amorphous Si 3 N 4 powder via a liquid-phase method, followed by a heat treatment process to realize tailorable shapes for α-Si 3 N 4 crystal, while Si 3 N 4 granules were synthesized and whiskers were inhibited. The influence of the temperature on the particle morphology and growth mechanisms were investigated. Characterizations indicated that the crystallization temperature played an important role in controlling the morphology of α-Si 3 N 4 powder, while the relatively lowly temperature (below 1519 °C) was beneficial to forming whiskers. The growth of Si 3 N 4 whiskers, derive from the reaction between SiO(g) and N 2 , follows the vapor–solid (VS) mechanism, which is inhibited by the increase in temperature. Instead, higher temperatures were conducive to forming granular structures. Highly crystalline α-Si 3 N 4 granules were prepared at 1550 °C due to Si*(g) and N*(g) recombining to Si 3 N 4 nuclei via the vapor–solid-solid (VSS) mechanism providing a theoretical basis for inhibiting the production of the whiskers.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kewei Zheng,Yuanyuan Wu,Qingfei Dai,Xiaojun Yan,Yu Liu,Di Sun,Zhongjie Yu,Shuoqi Jiang,Qingbao Ma,Wei Jiang
The objective of this study was to explore the potential of Antarctic krill-derived peptides as α-glucosidase inhibitors for the treatment of type 2 diabetes. The enzymolysis conditions of α-glucosidase inhibitory peptides were optimized by response surface methodology (RSM), a statistical method that efficiently determines optimal conditions with a limited number of experiments. Gel chromatography and LC-MS/MS techniques were utilized to determine the molecular weight (M w ) distribution and sequences of the hydrolysates. The identification and analysis of the mechanism behind α-glucosidase inhibitory peptides were conducted through conventional and computer-assisted techniques. The binding affinities between peptides and α-glucosidase were further validated using BLI (biolayer interferometry) assay. The results revealed that hydrolysates generated by neutrase exhibited the highest α-glucosidase inhibition rate. Optimal conditions for hydrolysis were determined to be an enzyme concentration of 6 × 10 3 U/g, hydrolysis time of 5.4 h, and hydrolysis temperature of 45 °C. Four peptides (LPFQR, PSFD, PSFDF, VPFPR) with strong binding affinities to the active site of α-glucosidase, primarily through hydrogen bonding and hydrophobic interactions. This study highlights the prospective utility of Antarctic krill-derived peptides in curtailing α-glucosidase activity, offering a theoretical foundation for the development of novel α-glucosidase inhibitors and related functional foods to enhance diabetes management.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Linwei Yu,Xi Li,Tianyi Shi,Ning Li,Donge Zhang,Xikai Liu,Yushuo Xiao,Xinran Liu,Robert B. Petersen,Weikang Xue,Yanxun V. Yu,De-sheng Hu,Li Xu,Hong Chen,Ling Zheng,Kun Huang,Anlin Peng
Parkinson's disease (PD), a neurodegenerative disorder without cure, is characterized by the pathological aggregation of α-synuclein (α-Syn) in Lewy bodies. Classic deposition pathway and condensation pathway contribute to α-Syn aggregation, and liquid-liquid phase separation is the driving force for condensate formation, which subsequently undergo liquid-solid phase separation to form toxic fibrils. Traditional Chinese Medicine (TCM) has a long history in treating neurodegenerative disease; herein, we identified chemicals from herbs that inhibit α-Syn aggregation. We screened commonly prescribed TCMs for PD from the CNKI database and registered patents, 13 chemicals were identified in the TCMSP databases as candidate inhibitors, among which three phenols, forsythoside B (FTSB), echinacoside (ECH), and 4-hydroxyindole (C4-OH) efficiently inhibit α-Syn aggregation. Moreover, FTSB and ECH increase α-Syn fluidity within condensates, inhibit α-Syn transition into amyloid fibrils and reduce fibril-induced toxicity in SH-SY5Y cells. Importantly, they disaggregated preformed α-Syn amyloid fibrils. Notably, in an α-Syn overexpressing NL5901 C. elegans PD model, either FTSB or ECH treatment significantly extended the lifespan and improved the PD-like movement disorders, both in the preventive and therapeutic treatment approaches, by reducing toxic α-Syn inclusion formation and improving the fluidity of α-Syn. Together, we offer new therapeutic candidates targeting phase separation-associated aggregation for PD.
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RSC Advances
·
2024
·Vol. 14
·期 24
·16649-16660
·
IF 3.9
Juan Wei,Chaoqun Yan,Yi Chen,Zhiliang Cheng,Facheng Qiu,Congming Tang,Cheng Yang,Zejun Wei,Anke Du
Currently, the surface structure modification of photocatalysts is one of the effective means of enhancing their photocatalytic efficiency. Therefore, it is critically important to gain a deeper understanding of how the surface of α-Fe2O3 photocatalysts influences catalytic activity at the nanoscale. In this work, α-Fe2O3 catalysts were prepared using the solvothermal method, and four distinct morphologies were investigated: hexagonal bipyramid (THB), cube (CB), hexagonal plate (HS), and spherical (RC). The results indicate that the hexagonal bipyramid (THB) exhibits the highest degradation activity towards tetracycline (TC), with a reaction rate constant of k = 0.0969 min−1. The apparent reaction rate constants for the cube (CB), hexagonal plate (HS), and spherical (RC) morphologies are 0.0824, 0.0726, and 0.0585 min−1, respectively. In addition, it has been observed that the enhancement of photocatalytic activity is closely related to the increase in surface area, which provides more opportunities for interactions between Fe2+ and holes. The quenching experiments and electron paramagnetic resonance (EPR) results indicate that the ˙O2, ˙OH and h+ contribute mainly to the degradation of TC in the system. This research contributes to a more comprehensive understanding of catalyst surface alterations and their impact on catalytic performance.
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Food Bioscience
·
2024
·
IF 4.8
Xiaoning Liu,Zekun Xu,Xingxun Liu,Chuangchuang Zhang,Mengting Ma,Zhongquan Sui,Harold Corke
Starch granule surface and channel proteins (GSCP) or starch granule channel proteins (GCP) were respectively removed from waxy and non-waxy rice starches . The effects of α-amylase hydrolysis on the lamellar structure changes of rice starches were examined by small-angle X-ray scattering (SAXS). SAXS revealed that the scattering peaks of hydrolyzed starch granules were broadened. The result was also evidenced by the decreased peak areas ( A ) and increased peak width at half-maximum ( W ). The α-amylase hydrolysis of starches could induce a transformation from a mass to a surface fractal and increase the compactness of starches. Moreover, the thickness of amorphous lamellae ( d a ) decreased and that of crystalline lamellae ( d c ) increased for starches after hydrolysis. Hydrolyzed starches exhibited a higher proportion of short-branch chains (fa) and long-branch chains (fb3) compared to their native form, indicating that α-amylase mainly attacked the long molecular chain of amylopectin with branch points in the amorphous lamellae. Removal of GSCP accelerated and exacerbated the decrease in d a and the increase in d c . Further, GSCP removal brought on the formation of less compact starch during long-time hydrolysis. Overall, the results demonstrated that GSCP removal had significant impacts on structural changes during α-amylase hydrolysis from lamellar to molecular scales.
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MATERIALS RESEARCH BULLETIN
·
2017
·
IF 2.446
Hua Liu,Zumin Wang,Haiyan Li,Hao Wang,Ranbo Yu
Industrial wastewater containing both organic and inorganic pollutants is being generated all the time. Efficient materials are highly needed to purify the polluted water. Thus, a hierarchical structured α-Fe 2 O 3 with excellent adsorptive property has been synthesized by using a facile hydrothermal method. It can be prepared in macroscopic quantity. The as-synthesized α-Fe 2 O 3 with silkworm cocoon-like morphology shows high efficiency in adsorbing Congo red (CR), Methyl orange (MO) and Cr(VI). The removal efficiency of CR, MO and Cr(VI) can reach 99.2%, 83.9% and 100% under appropriate conditions. Besides, this α-Fe 2 O 3 shows high adsorptive ability for mixed CR and Cr(VI) in aqueous solution and the final removal rates can reach 94.7% and 97.9%, respectively. The adsorption kinetics fits well with the pseudo-second-order kinetic model. The results indicate that the product may find potential application in purification of wastewater containing both organic and inorganic pollutants.
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Biomaterials Science
·
2018
·Vol. 6
·期 5
·1201-1216
·
IF 5.831
WenTing Li,JinRong Peng,Qian Yang,LiJuan Chen,Lan Zhang,XiaoXin Chen,ZhiYong Qian
Micellar nanoparticles have unique advantages as carriers for therapeutic or imaging agents, owing to their smaller size and better penetration of tumors. However, some agents, due to their physical or chemical properties, are difficult to load into micelles. IR780 is one of these agents, and is also a promising near-infrared dye for fluorescence imaging (FI)/photoacoustic imaging (PAI) and cancer photothermal therapy (PTT). Its hydrophobic and high crystallization structure results in limited bioavailability in vivo. It is difficult to load into micelles constructed from an amphiphilic block polymer with relatively low molecular weight. In this study, we use computer simulation and introduce another small biomolecule, α-lipoic acid, into the micelles constructed from a mPEG-PCL copolymer, to lower the energy of molecular interaction between MPEG-PCL and IR780, and expect to enhance the loading capacity of the micelles to IR780. The introduction of α-lipoic acid decreases the energy of molecular interaction between MEPG-PCL and IR780 from −46.18 kJ mol−1 to −196.52 kJ mol−1 and increases the loading capacity and stability of the mPEG-PCL micelles to IR780, which also maintains the loading capacity to DTX. We further construct DTX/IR780 co-loaded mPEG-PCL micelles for FI/PAI dual modal imaging guided PTT/chemotherapy of cancer. By FI and PAI evaluation in vitro and in vivo, we demonstrate that the DTX/IR780 co-loaded micelles can be used as FI and PAI probes. By further evaluating the therapeutic outcome of PTT/chemotherapy co-therapy of breast cancer, we demonstrate that the DTX/IR780 co-loaded mPEG-PCL micelles can serve as promising candidates for FI and PAI guided PTT/chemotherapy of breast cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qingxiang Wang,Min Liu,Jihong Zhao,Jiahao Yuan,Shasha Li,Ruijiang Liu
The prevention, diagnosis and treatment of cancer have always been the focus of medical research. In this study, a label-free, rapid, simple, sensitive, and specific method for the detection of HPV16 E7 oncoprotein was developed. The electrochemical biosensor platform was constructed by magnetic self-assembly of α-Fe 2 O 3 /Fe 3 O 4 @Au nanocomposites onto the surface of magnetic glass carbon electrode (MGCE), and the nanocomposite was connected to aptamer through Au S bond to construct a probe to capture HPV16 E7. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were applied to verify the performance of the constructed biosensor. The condition optimization and performance analysis were carried out by differential pulse voltammetry (DPV). Under optimal conditions, the biosensor exhibited a strong linearity between current value and the logarithm of HPV16 E7 oncoprotein concentration in the range of 10 pg/mL – 0.1 μg/mL, with a limit of detection (LOD) of 159 fg/mL and a limit of quantification (LOQ) of 530 fg/mL, and it also revealed excellent reproducibility, long-term stability, and anti-interference ability. In a word, the biosensor would contribute to the early diagnosis of oncoprotein HPV16 E7 and had promising application prospects.
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