Chem
·
2025
·
IF 19.6
Xinyuan Bi,Xiaohang Qian,Bingsen Xue,Miao Zhang,Shuyu Liu,Haoran Chen,Cheng Jin,Huidong Tang,Jian Ye
Multiplexed detection is a challenging yet essential task in analytical chemistry, especially for complex systems. Surface-enhanced Raman spectroscopy (SERS) is a promising analytical tool due to its molecular fingerprinting capability, sensitivity, low cost, and tractability. Considering the molecular profusion and diversity, SERSome, namely, spectral set, facilitates robust detection but is still challenged by spectral overlapping-induced uncertainty of molecular assignment and multiplexed quantification. Herein, we introduce molecule-resolvable (MORE) SERSome, identifying specific analytes contributing to the complex SERS spectra, which are then used in spectral decomposition for multiplexed analysis. Taking metabolic profiling for Alzheimer’s disease as a proof of concept, ten metabolites are screened in human serum. A deep-learning model enables accurate and rapid diagnosis, achieving an area under the receiver operating characteristic curve as high as 91.5%. Comparing with conventional methods, MORE SERSome presents a methodological advancement in multiplexed detection with strong potential for general applications and fundamental research in analytical chemistry.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xingwei Wang,Heping Cui,Xiaoming Zhang,Jingyang Yu,Shuqin Xia,Chi-Tang Ho
Directional and rapid formation of the Amadori rearrangement product (ARP) from the glutamic acid and xylose was achieved through intermittent microwave heating. The yield of ARP reached 58.09 % by subjecting the system to intermittent microwave heating at a power density of 10 W/g for 14 min. Dehydration rate and microwave effects were found to be key factors to optimize the conditions for directional and rapid preparation of the ARP. Through a comprehensive analysis of the ARP degradation and further browning under both conductive and microwave thermal processing, it was observed that microwave processing significantly accelerated the browning degree of systems, leading to a tenfold reduction in the heating time required for browning. This research presented a promising avenue for the development of novel and expedited methods for the production of ARP and highlighted the potential of ARP in enhancing color quality in fast-cooking applications utilizing microwave.
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NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Zhanghong Guo,Lin Zhou,Haining Cui,Jin-Xin Ma,Chan Wang,Qijun Song
Natural laccase, a member of the copper blue oxidase family, has its catalytic sites are classified as type1 (T1), type2 (T2) and type3 (T3) Cu. The electron transfer distance between the T1 to T2/T3 Cu has a great effect on the electron transfer efficiency in the laccase-catalyzed reaction. To mimic the active sites of laccase, a copper-doped carbon dots (CuCDs) was synthesized by a one-step hydrothermal treatment of copper sulfate and riboflavin. Experiment characterizations and theoretical simulations revealed that the catalytic sites in CuCDs consist of N, O coordinated copper and quinoxaline structure, which resemble the coordination environment of T2 Cu and the function of the T3 Cu center, respectively. The short electron transfer distance of 4.8 Å in catalytic sites of CuCDs accelerates the oxidation of the substrate dopamine (DA) to polydopamine (PDA) with a reaction rate being 40 times faster than that of laccase. Galvanic cell experiments proved that electron transfer from DA to oxygen was achieved in the presence of CuCDs. Therefore, this study not only could provide an efficient and eco-friendly nanozyme for hair dyeing in ambient condition, but also could inspire more and deeper researches in the field of mimicking natural enzyme with carbon nanomaterials.
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Heliyon
·
2024
·
IF 3.4
Yan Zhang,Hua Wei,Pingguang Zhu,Xiaojuan Hao,Jing Chen,Haina Zhang
The adhesion of bacteria to the surface leads to formation of biofilms causing numerous infection problems in implanting medical devices or interventional therapy. Traditional treatment for such problems is generally to administrate patients with antibiotics or antifungal agent. Alternatively, devices are taken out of the body to mechanically destroy the biofilm and re-used by surgery. In this study, a straightforward method was developed to remove biofilms using a MXene-based photothermal hydrogel. The hydrogel consists of dynamic crosslinking network formed by Schiff-base reaction between aldehyde-containing xyloglucan (OXG) and amine-containing MXene (NH 2 -MXene), which showed efficient killing of both gram-positive Staphylococcus aureus ( S. aureus ) and gram-negative Escherichia coli ( E. coli ) bacteria upon near-infrared (NIR) laser irradiation. The NH 2 -MXene/OXG nanocomposite hydrogel showed a high photothermal antibacterial efficiency and stable photothermal conversion, demonstrated by efficient removal of biofilms ex vivo .
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OPTICAL MATERIALS
·
2024
·
IF 3.8
Zhuoyuan Li,Yulin Wang,Jinting Chen,Li Zhang,Yongbiao Hua,Donglan Huang,Huishi Guo,Xiuzhen Qiu
Tetracycline (TC) is a typical emerging pharmaceuticals and personal care products (PPCPs) pollutants in the environment. However, TC residues cause severe effects on human health. It is crucial to exploit simple, rapid and sensitive methods to detect TC in water samples. A ratiometric fluorescent probes (CDs-Eu 3+ ) is constructed using carbon dots (CDs) and europium ions (Eu 3+ ) for the visual detection of tetracycline (TC). Under 390 nm excitation, CDs-Eu 3+ shows a strong yellow fluorescence at 545 nm and a weak red fluorescence at 619 nm, respectively. In the presence of TC, the fluorescence intensity at 545 nm decreased while the fluorescence intensity at 619 nm increased, which attributed to the internal filtering effect (IFE) and “antenna effect”, respectively. Sensing performance of CDs-Eu 3+ probes is further evaluated with respect to various conditions, including salt concentration, Eu 3+ concentration, pH, and reaction time. Results reveal that the proposed ratiometric fluorescent probes exhibits a specific recognition ability toward TC. An excellent linear relationship is observed between the fluorescence intensity ratio (F 619 /F 545 ) and TC concentrations (1⁓100 μM), with a low detection limit (LOD) of 31 nM. The solution (CDs-Eu 3+ -TC) color gradually changes from yellow to red after adding TC. The probes are successfully applied to determine TC in water with recoveries of 85.43⁓97.53%. Finally, a fluorescent test strip is prepared and used for rapid visual semi-quantitative TC detection.
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HYDROMETALLURGY
·
2024
·
IF 4.8
Huaixin Hao,Yaoyang Liu,Jianhua Yuan,Xue Dong,Zhipeng Wang,Chao Xu,Jing Chen
Cerium (Ce) has become an important strategic resource in high-tech fields owing to its excellent optical, magnetic, acoustic, and electrical properties. The recovery of non-natural Ce derived from nuclear fission through spent fuel reprocessing is of potential significance for the reservation of extremely precious resources. In the present work, Ce 3+ was oxidized to Ce 4+ by NaBiO 3 and then extracted by alkyl phosphate ligands. The extraction efficiency can be enhanced by increasing acidity, raising extractant concentration and decreasing temperature. By taking the advantage of the intrinsic selectivity to tetravalent lanthanides (Ln) by alkyl phosphates, highly efficient and selective extraction of Ce 4+ from other trivalent lanthanides with remarkable separation factor ( SF ) over 10 3 was achieved. Meanwhile, the back extraction of Ce could be accomplished through reducing Ce 4+ to Ce 3+ by H 2 O 2 . Further studies on structure-property relationship by spectroscopic methods and DFT calculations revealed that alkyl substituent groups with more C atoms enhanced the complexation between Ce 4+ and the ligand, while steric hindrance effect could weaken the extractability of Ce 4+ . Moreover, a shorter Ce O bond and a stronger covalent interaction in the Ce 4+ complex, as compared to the Ln 3+ complex, contributed to the excellent selectivity to Ce 4+ over Ln 3+ .
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BIOCHEMICAL PHARMACOLOGY
·
2024
·
IF 5.3
Shan Meng,Xin Chen,Jikai Zhao,Xinyi Huang,Yuting Huang,Tao Huang,Zijun Zhou,Wenpu Ren,Tao Hong,Jinfeng Duan,Liming Yu,Huishan Wang
Fibronectin type III domain-containing protein 5 (FNDC5) exerts potential anti-arrhythmic effects. However, the function and mechanism of FNDC5 in diabetes-associated atrial fibrillation (AF) remain unknown. In this study, bioinformatics analysis, in vivo and in vitro experiments were conducted to explore the alteration and role of FNDC5 in diabetes-related atrial remodeling and AF susceptibility. RNA sequencing data from atrial samples of permanent AF patients and diabetic mice exhibited significantly decreased FNDC5 at the transcriptional level, which was in line with the protein expression in diabetic mice as well as high glucose and palmitic acid (HG+PA) injured atrial myocytes. Diabetic mice exhibited adverse atrial remodeling and increased AF inducibility. Moreover, reduced atrial FNDC5 was accompanied with exacerbated NOD-like receptor pyrin domain containing 3 (NLRP3) activation and disturbed mitochondrial fission and fusion processes, as evidenced by decreased expressions of optic atrophy 1 (OPA-1), mitofusin (MFN-1, MFN-2) and increased phosphorylation of dynamin-related protein 1 (Ser616). These effects were validated in HG+PA-treated atrial myocytes. Critically, FNDC5 overexpression remarkably enhanced cellular antioxidant capacity by upregulating the expressions of superoxide dismutase (SOD1, SOD2) level. In addition, HG+PA-induced mitochondrial dysfunction was ameliorated by FNDC5 overexpression as evidenced by improved mitochondrial dynamics and membrane potential. Moreover, NLRP3 inflammasome-mediated inflammation was reduced by FNDC5 overexpression, and AMPK signaling might serve as the key down-stream effector. The present study demonstrated that reduced atrial FNDC5-AMPK signaling contributed to the pathogenesis of diabetes- associated AF by impairing mitochondrial dynamics and activating the NLRP3 inflammasome. These findings provide promising therapeutic avenues for diabetes-associated AF.
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Food Bioscience
·
2024
·
IF 4.8
Xin-Yu Zu,Ya-Nan Zhao,Yan Liang,Ying-Qiu Li,Chen-Ying Wang,Xiang-Zhong Zhao,Hua Wang
The rapid screening of angiotensin I-converting enzyme (ACE) inhibitory peptides (ACEIPs) from protein hydrolysates remains a challenge in current researches. The current study presents the development of an efficient affinity medium (AOPAN–ACE) consisting of ACE immobilized on the surface of electrostatically spun polyacrylonitrile nanofibrous membranes for the efficient screening of ACEIPs. The application of this affinity medium followed by liquid chromatography–tandem mass spectrometry analysis resulted in the successful screening and identification of 60 potential ACEIPs from protein hydrolysates of tuna dark muscle. Notably, the application of PeptideRanker, pLM4ACE, and molecular docking approaches resulted in the screening and identification of a novel ACEIP FPPDVA. Furthermore, the peptide FPPDVA was synthesized using the solid-phase method, and its IC 50 value on ACE was determined to be 87.11 ± 1.02 μM. Molecular docking analysis suggested that the ACEIP of the peptide FPPDVA is attributable to the formation of hydrogen bonds with the S1 active site (Ala354 and Tyr523) of ACE and metal–ligand bonds with Zn 2+ . In addition, molecular dynamics simulations revealed that the peptide FPPDVA formed a stable complex with ACE and was consistently localized within the active pocket of ACE over a simulation duration of 100 ns without being displaced from the active site. Lineweaver–Burk analysis further confirmed that the peptide FPPDVA exhibited mixed mode of inhibition against ACE. Overall, the affinity medium developed in the current study is potentially useful for highly efficient screening of ACEIPs, and the novel ACEIP FPPDVA identified herein is a promising functional food ingredient for antihypertensive application.
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MICROCHIMICA ACTA
·
2024
·Vol. 192
·期 1
·1-12
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IF 5.3
Huang Yuanding,Chen Ping,Niu Meng,Peng Weng Kung
Clinical and immunological assays of white blood cells (WBCs) in human peripheral blood are of significance for disease diagnosis and immunological studies. However, separating WBCs from blood with high recovery and high purity remains challenging. In this study, by incorporating a pair of linearly tapered filter arrays, a crossflow filtration-based microfluidic chip was designed and fabricated for separation of WBCs from blood. The implementation of the linearly tapered filter arrays not only ensures a minimal and consistent flow through each sieve, but also achieves a high filtration ratio (~ 19). The validity and robustness of this straightforward design were substantiated through theoretical analysis, simulations, and model microparticle tests. The microfluidic chip achieved an almost perfect (> 99.2%) recovery and a ~ 20-fold enrichment of the targeted 8 μ m particles (as surrogates for WBCs) from undesired 2 μ m particles (as substitutes for red blood cells, RBCs) at flow rates ranging from 50 to 200 μ L/min, irrespective of the filter array length and particle concentration. When applied to a twenty times diluted blood sample, the chip achieved a 96.6% recovery and 19.7-fold enrichment of WBCs, as well as a 95.0% removal of RBCs, at the optimal flow rate of 100 μ L/min. With its simple design, cost-effectiveness, high recovery, substantial enrichment ratio, and considerable throughput, this chip offers an alternative solution that is potentially applicable to scenarios involving the separation of WBCs as well as other particles/cells. Graphical abstract
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INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2024
·
IF 4.1
Liwen Zhang,Luyao Xi,Shuyu He,Huizi Wen,Shanyi Tan,Shaohua Chen
Bioremediation has garnered considerable interest due to its advantages of economy and no secondary pollution. However, the direct electron transfer rate between microorganisms and Cr(VI) is low. The bioreduction of Cr(VI) by Shewanella oneidensis MR-1 ( S. oneidensis ) in the presence of formylmethylflavin (FMF) was conducted to understand how FMF mediated the extracellular electron transfer process to improve Cr(VI) removal. In this study, FMF was firstly synthesized by modifing RF to increase its solubility and redox activity. The findings indicate that S. oneidensis /FMF exhibited better Cr(VI) removal performance compared to that of S. oneidensis . Under optimum conditions, 40 mg/L Cr(VI) could be completely removed by S. oneidensis /FMF within 120 h, while only 48.6% of Cr(VI) was removed by S. oneidensis , and the first order rate constant (k) for the Cr(VI) elimination by S. oneidensis /FMF (0.033 h −1 ) was about 4.1-fold greater than that of S. oneidensis (0.008 h −1 ). Moreover, the removal of Cr(VI) by S. oneidensis /FMF were dominated by reduction, and supplemented by adsorption and complexation. FMF enhance the extracellular electron transfer rate (EETR) was confirmed by electrochemical cyclic voltammetry (CV) and linear scanning voltammetry (LSV) experiments. This study emphasizes the potential important role of FMF in environmental bioremediation.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Chunte Feng,Hongfei Gao,Yang Yang,Xinyu Yang,Hao Tian,Huiyan Gu,Lei Yang
In this study, an acid-resistant magnetic boron-modified molecularly imprinted polymer (Fe 3 O 4 @NH 2 @BA@Lyz-MIPs) was prepared by the phase transition of lysozyme and used for the selective recognition and separation of rosmarinic acid from Perilla frutescens leaf extract. The synthesized materials were characterized by thermogravimetric analysis (TG), infrared spectroscopy (FT-IR), electron microscopy (SEM), and vibrating sample magnetometry (VSM). The adsorption and desorption experiments, adsorption kinetics experiments, and adsorption thermodynamics experiments revelated that the Fe 3 O 4 @NH 2 @BA@Lyz-MIPs exhibited a better adsorption capacity for rosmarinic acid (6.31 mg/g). Furthermore, Fe 3 O 4 @NH 2 @BA@Lyz-MIPs still exhibit a good adsorption performance after 8 adsorption-desorption cycles. The adsorption capacity decreased by 8.12 %, and the desorption performance decreased to 78.84 %. Stability experiments showed that the prepared imprinted layer was not destroyed by the eluent and could protect the internal magnetic core of the material. In summary, the prepared molecularly imprinted polymer Fe 3 O 4 @NH 2 @BA@Lyz-MIPs has good selective adsorption and high acid stability and can be swiftly retrieved from solution with the aid of an applied magnetic field, which provides a rapid and environmentally friendly strategy for the separation of rosmarinic acid from a complicated matrix.
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PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2024
·
IF 2.6
Jingmou Yu,Mengqi Liu,Chao Zhang,Lizhen Cheng,Changchun Peng,Dengzhao Jiang,Wenbo Liu,Hongguang Jin,Jin Ren
Herein, we designed and synthesized novel reactive oxygen species (ROS)-responsive glycol chitosan-doxorubicin (DOX) prodrug via a ROS-cleavable thioketal (TK) linker. The obtained GC-TK-DOX formed self-assembled nanoparticles of 312 nm in aqueous media. Photosensitizers zinc phthalocyanine (ZnPc)-loaded GC-TK-DOX (GC-TK-DOX/ZnPc) nanoparticles were fabricated by using a dialysis approach. The GC-TK-DOX and GC-TK-DOX/ZnPc nanoparticles were nearly spherical by transmission electron microscopy (TEM) observation. Under 660-nm laser irradiation, GC-TK-DOX/ZnPc could generate singlet oxygen. Further, GC-TK-DOX/ZnPc nanoparticles exhibited ROS-sensitive release of DOX and ZnPc in vitro. GC-TK-DOX/ZnPc with laser irradiation showed more drug uptake and higher cytotoxic effects than GC-TK-DOX/ZnPc without irradiation, free DOX and GC-TK-DOX in HeLa tumor cells. Overall, these findings suggested that GC-TK-DOX/ZnPc could be a promising nanoarchitecture for synergetic chemo-photodynamic therapy against tumors.
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