ACS Sensors
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2021
·
IF 7.711
Xiaofang Wang,Tianci Xu,Yue Zhang,Nan Gao,Taotao Feng,Shujun Wang,Meining Zhang
Electrochemical tracking of redox-inactive neurochemicals remain a challenge due to chemical inertness, almost no Faraday electron transfer for these species, and the complex brain atmosphere. In this work, we demonstrate a low-cost, simple-making liquid/liquid interface microsensor (LLIM) to monitor redox-inactive neurochemicals in the rat brain. Taking choline (Ch) as an example, based on the difference in solvation energies of Ch in cerebrospinal fluid (aqueous phase) and 1,2-dichloroethane (1,2-DCE; organic phase), Ch is recognized in the specific ion-transfer potential and distinctive ion-transfer current signals. The LLIM has an excellent response to Ch with good linearity and selectivity, and the detection limit is 0.37 μM. The LLIM can monitor the dynamics of Ch in the cortex of the rat brain by both local microinfusion and intraperitoneal injection of Ch. This work first demonstrates that the LLIM can be successfully applied in the brain and obtain electrochemical signals in such a sophisticated system, allowing one new perspective of sensing at the liquid/liquid interface for nonelectrically active substances in vivo to understand the physiological function of the brain.
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Frontiers in Pharmacology
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2021
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IF 5.811
Hao Xincai,Sun Xiaodong,Zhu Haizhen,Xie Lixia,Wang Xuanbin,Jiang Nan,Fu Pan,Sang Ming
Trans-resveratrol (RES) exhibits a wide range of biological activities. Various methodological approaches have been established to improve the pharmacokinetic properties of RES. Moreover, additional in vivo studies are required to support clinical application. In this study, RES/HP-β-CD (RHSD) inclusion complex was prepared and characterized by FTIR, PXRD, DSC and NMR data. The effect and potential mechanism of RHSD against cervical cancer were investigated in a mouse xenograft tumor model by qPCR assay, Western blot assay, and immunohistochemical assay. Results showed that RHSD significantly decreased tumor growth compared with free RES, while the effect of preventing tumor growth was more prominent in vivo. Notably, RHSD could inhibit tumor development by suppressing the expression of HPV E6 and E7 oncogenes and upregulating P53 and Rb1 protein in cervical cancer. These findings demonstrated that RHSD was safe and potential for development of a new oral administration drug to treat cervical cancer.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2021
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IF 5.316
Jingjing Zheng,Xiaotian Li,Bo Zhang,Xiaoxin Zou,Qihua Liang,Hui Chen,Guo-Dong Li
The concentration of acetone in exhaled breath is an efficient indicator for the diagnosis of diabetes. However, it is difficult to detect acetone in exhaled breath due to the extremely low acetone concentration, rather complex composition and very high humidity. Herein, we report Pt-decorated foam-like Ga-In bimetal oxide nanofibers (Pt-GIO) for trace concentration acetone detection. We found that the Pt-GIO based sensor exhibits enhanced sensing response towards acetone compared with that of Ga-In bimetal oxide (GIO), due to the formation of Schottky contact between Pt and n-type GIO, leading to the increased chemisorbed oxygen. As a result, the Pt-GIO based sensors gives a high response (3.2), short response time (13 s) to 1.8 ppm acetone and very low detection limit (300 ppb). The Pt-GIO sensor also gives good stability and is nearly insensitive to moisture in the range of 40% RH to 95%. These advantages including high response, low detection limit, short response time and high moisture resistance endow Pt-GIO a promising sensing material for acetone detection in exhaled breath.
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Food & Function
·
2021
·Vol. 12
·期 16
·7440-7447
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IF 5.396
Ying Li,Bo Chen,Hai-Yan Cao,Jing-En Li,Ling-Li Chen,Qing-Feng Zhang
Bambusa multiplex cv Fernleaf (B. multiplex) is a species of bamboo. In the present study, B. multiplex leaf extract was prepared through the resin absorption/desorption procedure and analyzed by HPLC. C-Glycosyl flavonoids are the main constituents of B. multiplex extract, and the content of isoorientin and vitexin was 51.8 and 23.1 mg g−1, respectively. Besides, the extract exhibited inhibitory activities on pancreatic lipase and α-glucosidase with IC50 values of 0.91 and 1.16 mg mL−1, respectively. The extract could bind to pancreatic lipase and showed mixed-type inhibition. An in vivo study showed that pre-administration of B. multiplex extract significantly reduced the fat absorption in rats and increased fat excretion through feces. The change in the C-glycosyl flavonoid content in feces was the same as that in the triglyceride content. The inhibitory activity of B. multiplex leaf extract on pancreatic lipase was confirmed both in vitro and in vivo.
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INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2021
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IF 4.32
Zhendong Wu,Yimin Pan,Fengmin Ma,Qiaoqiao Ren,Jiguo Wu,Yunxia Jiang,Yumei He,Yingxin Yu,Guoxia Zhang
The gut microbiome is crucial for the health of animals including human and is sensitive to environmental factors. Dechlorane Plus (DP) is a widely used polychlorinated flame retardant in commercial products for which general human population has been exposed to, but its effects on the gut microbiota are largely unknown. In this work, experiments were conducted to examine the effect of DP exposure on the intestinal microbiota by using Sprague-Dawley rats and a simulator of the human intestinal microbial ecosystem (SHIME). 16S rRNA gene high-throughput sequencing revealed that DP exposure in vivo reduced the relative abundance of the genera Prevotella , Quinella , and Ruminococcus , exposure in vitro decreased the relative abundance of Coriobacteriaceae , Parabacteroides , Bilophila , Sporolactobacillus , and Klebsiella . The phyla Synergistetes and Lentisphaerae were only detected in the descending colon . DP exposure also increased the concentrations of short-chain fatty acids (SCFAs) produced by the gut microbe both in vivo and in vitro, but SCFA metabolism was gradually inhibited as the increase of DP concentrations. These results indicated that DP exposure changed both the structure and metabolic function of the gut microbiota, suggesting more attention should be concentrated on the health effects of DP exposure, especially at higher doses.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Si-Si Liu,Jin-Ling Li,Lin-Ke Ge,Cai-Lin Li,Jian-Liang Zhao,Qian-Qian Zhang,Guang-Guo Ying,Chang-Er Chen
Fluoroquinolones (FQs) have raised significant concerns due to their ubiquitous occurrence and promoting of antimicrobial resistance (AMR). In this study, a molecularly imprinted polymer-diffusive gradient in thin-films (MIP-DGT) sampler is developed for selective measurement of FQs in waters by using a commercial available MIP material as the binding agent. The MIP-DGT shows selective adsorption of the FQs and linearly accumulates the FQs over the deployment time. MIP-DGT measurement is independent of pH (6–8) and ionic strength (IS) (0.01–0.5 M) but is affected by DOM at higher concentrations (~10 mg•L −1 ), which is due to the altered diffusion coefficients and reduced adsorption on the MIP binding gel. Significant interaction effects of DOM with pH or IS indicate that this is the predominant influence on the MIP-DGT performance, which results in a lower measurement by the MIP-DGT but this is curtailed to some extend with increasing IS or pH. The MIP-DGT measurements, however, correlate well with those by grab sampling in a wastewater treatment plant , suggesting it is reliable for measuring FQs in waters. For the first time, we demonstrate that key water chemistry parameters do have interaction effects on the DGT measurements, which should be considered for the data interpretation. The MIP-DGT is a promising tool to understand the interaction effects of the environmental parameters on the fate, behaviours and bioavailability/toxicity of organic contaminants and improve environmental risk assessments in the environment and modelling.
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JOURNAL OF BIOMATERIALS APPLICATIONS
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2021
·
IF 2.646
Jinhua Hu,Bin Ai,Shibo Zhu,Zhen Wang,Huimin Xia,Wei Jia
To investigate the biocompatibility of polylactic acid-glycolic acid copolymer (PLGA) and PLGA/gelatin scaffolds and their suitability for tubular urethral replacement in a canine model. PLGA and PLGA/gelatin scaffolds was constructed by electrospinning. Microstructural differences between the scaffolds was examined by Scanning electron microscopy (SEM) followed by mechanical properties testing. Biocompatibility of the material was evaluated using SEM 4, 8, 12 and 72 h after PLGA and PLGA/gelatin scaffolds co-culture with urothelial cells. And confocal analysis was also used to showed the cell adhesive and growth at 12 h. Approximately 2 cm of the anterior urethra of twelve dogs were removed and replaced with a scaffold. After the surgery for 1 month performed urethrography and for 3 month perform hematoxylin–eosin (H&E) and Masson. The results indicated that PLGA and PLGA/gelatin scaffolds had a void microfilament structure, similar to that of normal acellular matrix tissue. And the tensile strength was decreased whereas the tensile deformation and suture retention strength was increased in PLGA/gelatin scaffolds compared to that in PLGA scaffolds Urothelial cells grew well on both scaffolds. Postoperatively, animals recovered well and urinated spontaneously. However, urethrography showed varying degrees of urethral strictures in the reconstructed urethras. H&E and Masson showed that multilayer urothelial cells were formed in both the proximal and distal segments of the reconstructed urethras but without continuity. There was a small amount of smooth muscle and blood vessels under the epithelium, but regenerative urothelial cells at the midpoint of the reconstructed segment did not continue. Lots of lymphocyte infiltration was observed under the epithelium, some collagen tissue was deposited under the neo-urethral epithelium were observed. In conclusion, PLGA and PLGA/gelatin scaffolds are not suitable for tubularized urethral replacement in the canine model.
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BMC MICROBIOLOGY
·
2021
·Vol. 21
·期 1
·1-12
·
IF 3.605
Miao Z. H.,Zhou W. X.,Cheng R. Y.,Liang H. J.,Jiang F. L.,Shen X.,Lu J. H.,Li M.,He F.
Background Accumulating evidence have shown that the intestinal microbiota plays an important role in prevention of host obesity and metabolism disorders. Recent studies also demonstrate that early life is the key time for the colonization of intestinal microbes in host. However, there are few studies focusing on possible association between intestinal microbiota in the early life and metabolism in adulthood. Therefore the present study was conducted to examine whether the short term antibiotic and/or probiotic exposure in early life could affect intestinal microbes and their possible long term effects on host metabolism.Results A high-fat diet resulted in glucose and lipid metabolism disorders with higher levels of visceral fat rate, insulin-resistance indices, and leptin. Exposure to ceftriaxone in early life aggravated the negative influences of a high-fat diet on mouse physiology. Orally fed TMC3115 protected mice, especially those who had received treatment throughout the whole study, from damage due to a high-fat diet, such as increases in levels of fasting blood glucose and serum levels of insulin, leptin, and IR indices. Exposure to ceftriaxone during the first 2 weeks of life was linked to dysbiosis of the fecal microbiota with a significant decrease in the species richness and diversity. However, the influence of orally fed ceftriaxone on the fecal microbiota was limited to 12 weeks after the termination of treatment. Of note, at week 12 there were still some differences in the composition of intestinal microbiota between mice provided with high fat diet and antibiotic exposure and those only fed a high fat diet.Conclusion sThese results indicated that exposure to antibiotics, such as ceftriaxone, in early life may aggravate the negative influences of a high-fat diet on the physiology of the host animal. These results also suggest that the crosstalk between the host and their intestinal microbiota in early life may be more important than that in adulthood, even though the same intestinal microbes are present in adulthood.
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TRIBOLOGY INTERNATIONAL
·
2021
·
IF 4.872
Lisong Dong,Jian Wu,Danyang Cao,Xin Feng,Jiahua Zhu,Xiaohua Lu,Liwen Mu
Liquid metal (LM) can be well distributed in polyimide (PI) coatings by ultrasonic shear into 1–2 μm diameter. The LM incorporated PI (PI-LM) coatings show remarkably enhanced tribological properties when the coating slide against GCr15 steel ball at room temperature, while the mechanical properties are slightly reduced. When the filling amount is 8%, the wear is just 10% of pure PI and the friction coefficient can be reduced by 31.6%. During friction process , the LM microspheres are squeezed to the frictional interface and form a transfer film together with PI debris, achieving lower wear and friction coefficient. These results show that PI-LM coatings have high potential as advanced self-lubricating materials.
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GEODERMA
·
2021
·
IF 6.114
Bai-Lin Liu,Yan-Wen Li,Jing-Jie Guo,Lei Xiang,Hai-Ming Zhao,Nai-Xian Feng,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming Hung Wong
A better understanding of microcystin elimination in soil will boost the development of effective strategies to mitigate associated contamination. This study addressed the role and mechanism by which earthworm Eisenia fetida take part in the elimination of microcystin-LR (MC-LR) in soil. The degradation kinetics of MC-LR exhibited good fits with the availability-adjusted first-order kinetic model ( R 2 > 0.97), where >71% of the toxin was rapidly eliminated within the initial eight days ( t 1/2 = 2.34–3.07 days). E . fetida significantly accelerated MC-LR elimination, with a less t 1/2 of 1.09–1.12 days; however, E . fetida did not further improve the final elimination efficiencies on Day 29 (82.59–93.57% with E . fetida vs . 82.96–92.34% without E . fetida ). In soil, E . fetida accelerated MC-LR elimination mainly via biodegradation arising from elevated total organic carbon, enhanced enzyme activities (urease and sucrase) and ameliorations of bacterial bioactivity, diversity and abundance. Indigenous bacteria of Methylophilus and Methylovorus , as well as earthworm-derived Flavobacterium , were considered as potential bacterial degraders for MC-LR. These findings elaborate on the variations in soil properties and soil bacterial community structure and diversity that occurs during MC-LR elimination and lay a foundation for controlling MC-LR contamination in the soil environment.
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JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 2
·546-562
·
IF 3.167
Yadi Liu,Guoxiao Lai,Yinrui Guo,Xiaocui Tang,Ou Shuai,Yizhen Xie,Qingping Wu,Diling Chen,Xujiang Yuan
Previous research has shown that the extracts from the Ganoderma lucidum spore (GS) have potentially cardioprotective effects, but there is still abundant room for development in determining its mechanism. In this study, the rat model of cardiac dysfunction was established by intraperitoneal injection of trimethylamine- N -oxide (TMAO), and the extracts of GS (oil, lipophilic components, and polysaccharides) were given intragastrically at a dose of 50 mg/kg/day to screen the pharmacological active components of GS. After 50 days of treatments, we found that the extraction from GS reduced the levels of total cholesterol, triglyceride, and low-density lipoprotein; increased the levels of high-density lipoprotein; and reduced the levels of serum TMAO when compared to the model group ( P < 0.05); especially the GS polysaccharides (DT) and GS lipophilic components (XF) exhibited decreases in serum TMAO compared to TMAO-induced control. The results of 16S rRNA sequencing showed that GS could change the gut microbiota, increasing the abundance of Firmicutes and Proteobacteria in the DT-treated group and XF-treated group, while reducing the abundance of Actinobacteria and Tenericutes . Quantitative proteomics analysis showed that GS extracts (DT and XF) could regulate the expression of some related proteins, such as Ucp1 (XF-TMAO/M-TMAO ratio is 2.76), Mpz (8.52), Fasn (2.39), Nefl (1.85), Mtnd5 (0.83), Mtnd2 (0.36), S100a8 (0.69), S100a9 (0.70), and Bdh1 (0.72). The results showed that XF can maintain the metabolic balance and function of the heart by regulating the expression of some proteins related to cardiovascular disease, and DT can reduce the risk of cardiovascular diseases by targeting gut microbiota.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2020
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IF 5.647
Yong Liu,Jingfu Wang,Jingan Chen,Zuxue Jin,Shiming Ding,Xiaohong Yang
The phosphate oxygen isotope (δ 18 O P ) ratio has been proven to be an effective tool to trace the sources and biogeochemical cycles of phosphorus (P) in aquatic ecosystems . However, the enrichment of phosphate (PO 4 ) and the removal of impurities are quite complex and easy to cause PO 4 loss in current δ 18 O P analytical methods. Moreover, the δ 18 O P value obtained by the commonly-used instantaneous sampling method is more of the instantaneous information of P, which is accidental or uncertain for accurate identification of the P source. In this study, a new method of in situ enrichment, elution, and purification of PO 4 (ISEEP) was developed for δ 18 O P analysis in waters. This method utilized a PO 4 binding phase (Zr-Oxide gel) to selectively in situ adsorb PO 4 in water and exhibited an adsorption capacity per unit area of up to 789.3 μg P/cm 2 . The PO 4 on the gel was eluted easily with a 1 M NaOH solution. More than 99.7% of the common anions, cations, and dissolved organic matter (DOM), as well as more than 90% of the trace elements were removed synchronously after adsorption and elution of PO 4 . The recovery rate of PO 4 in the whole procedure was as high as 92.8%. The XRD and SEM examinations showed that the ISEEP can obtain high-purity Ag 3 PO 4 solid for the δ 18 O P measurement. The reliability of the ISEEP method is confirmed by the measured δ 18 O P value and standard deviation of parallel samples from different types of natural waters obtained by both the ISEEP and the current popular McLaughlin (2004) method. It provides a good prospect of this new method for tracing the P sources and their biogeochemical cycling in aquatic ecosystems.
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