INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wenmin Wu,Qixing Jiang,Pei Gao,Dawei Yu,Peipei Yu,Wenshui Xia
The effects of the L-hisdine (L-His)-assisted ultrasound on physicochemical characteristics and conformation of myofibrillar protein (MP) under reduced-salt condition were investigated using spectroscopic analysis , and the binding mechanism between L-His and MP was further elucidated through molecular docking and molecular dynamics (MD) simulations. UV second derivative spectra and intrinsic Try fluorescence spectra revealed that L-His formed a complex with MP and altered the microenvironment of MP. After L-His-assisted ultrasound treatment, MP showed smaller particle size, higher solubility, and more uniform atomic force microscopy image due to the decrease of α -helix content and the subsequent increase in zeta potential , active sulfhydryl content, and surface hydrophobicity . Molecular docking and MD simulations demonstrated the optimal docking pose (minimum binding affinity of −6.78 kcal/mol) and revealed hydrophobic interactions and hydrogen bonds as the main interaction forces between L-His and MP, with several residues (ILE-464, ILE-480, THR-483, ASN-484, GLY-466, ASP-463, PHE-246) identified as binding sites.
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PubMed
高影响因子
POLYMER BULLETIN
·
2021
·Vol. 79
·期 3
·1615-1626
·
IF 2.87
Yang Rui,Hao Boran,Zhang Kan
The cleavage of the oxazine ring of benzoxazine resins generally requires high energy during the ring-opening polymerization, which leads them to be processed at a much higher temperature than the traditional thermoplastics. In this paper, a bio-based substance, L-histidine, has been used as the catalyst to lower the ring-opening polymerization of the well-commercialized benzoxazine resin (BA-a) based on aniline and bisphenol-A. The catalytic effect of L-histidine on the polymerization behaviors of benzoxazine resin (BA-a) has been monitored by differential scanning calorimetry and in situ Fourier transform infrared spectroscopy. In addition, L-histidine has also been found to be a good property modifier for BA-a-derived thermosets. The thermal properties of resulting polybenzoxazines have been investigated by thermogravimetric analysis and dynamic thermomechanical analysis. Notably, the thermoset derived from BA-a with adding only 5 mol% L-histidine exhibits excellent thermal stability in consideration of high T g (206 °C), high T d10 temperature (319 °C) and high chair yield value (40%), making this thermosetting resin a promising material for high-performance applications.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yu Zhang,Minhua Wei,Guihong Zhao,Wenjie Zhang,Yingzi Li,Beibei Lin,Yanjun Li,Qingyang Xu,Ning Chen,Chenglin Zhang
l -Homoserine is a valuable non-proteinogenic amino acid used in the synthesis of various important compounds. Microbial fermentation has potential value for producing l -homoserine on a large scale, but suffers from a low yield and the need for expensive additives. In this study, a non-induced, non-auxotrophic, plasmid-free Escherichia coli chassis for the high-efficiency production of l -homoserine was constructed. Initially, the l -homoserine degradation pathway was dynamically attenuated. Subsequently, systems metabolic engineering strategies were employed, including reinforcing the synthetic flux, improving NADPH generation, and elevating l -homoserine efflux. The constructed strain HOM-14, produced 60.1 g/L l -homoserine without additional supplements or inducers, which achieved the highest fermentative production efficiency of l -homoserine till date. Moreover, common byproducts, such as acetate, did not accumulate. The strategies presented here can be applied in the further engineering of chassis for the scale-up production of l -homoserine and derivatives.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zhiyu Xiong,Tong Shi,Wei Zhang,Yunfei Kong,Li Yuan,Ruichang Gao
The combined effects of l -arginine (Arg) and oxidized caffeic acid (OCF) treatment under different salt concentrations on gelling properties of bighead carp ( Aristichthys nobilis ) surimi gels were investigated. The results showed that the improvement of gel strength and water-holding capacity (WHC) of low-salt (0.5%) surimi gels could be obtained by adding Arg at various levels (0.1–0.4%) rather than OCF (0.1–0.9%, based on protein content). However, the gel strength and WHC of the low-salt surimi gel added with low-dose (0.1%) Arg alone were still far lower than that of regular-salt (3.0%) control. Whereas the significant improvements in low-salt surimi gels could be achieved when 0.1% Arg was combined with various levels of OCF, especially in combination with 0.5% OCF (LC-A-O). The water distribution in LC-A-O was markedly improved and its β-sheet structure content was significantly increased compared with the low-salt control ( P < 0.05). Moreover, a denser and more uniform network structure of LC-A-O was obtained, which was probably due to much stronger and more intra-molecular cross-linking of proteins, via the formation of new adducts between OCF and surimi proteins. Hence, the combined treatment of Arg and OCF showed a potential for improving the gel properties of low-salt surimi.
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高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Shuai Zhu,Ruizhong Xue,Zikun Yu,Xu Zhang,Shifang Luan,Haoyu Tang
Poly(l-cysteine)s with methylthio pendants (PMTLCs) were synthesized by ring-opening polymerization of a new l-cysteine-based N-carboxyanhydride. The thioether bonds of PMTLC can be readily oxidized by H2O2 yielding water-soluble PMTLCOX. The methylthio groups can undergo an alkylation reaction using methyl iodide and a subsequent ion-exchange reaction yielding sulfonium-based polypeptides (PPLC-DMS-X, where X = I, BF4). PPLC-DMS-X showed upper critical solution temperature-type thermo- and oxidation-responsive properties in aqueous solutions. Both PMTLC and PPLC-DMS-X showed oxidation-induced β-sheet to α-helix transitions. The absorbance of PPLC-DMS-I and methyl orange aqueous solution displayed a significant linear correlation with temperature, which makes the sulfonium-based polypeptides good candidates in the field of temperature sensors.
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高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 7
·e15604
·
IF 2.19
Cheng Peng,Rui Li,Hui Ni,Li Jun Li,Qing Biao Li
The naringinase, a desirable candidate for citrus juice debittering, consists of α-L-rhamnosidase and β-D-glucosidase. This study aimed to elucidate the effects of α-L-rhamnosidase, β-D-glucosidase, and their combination on naringin, polyphenols, antioxidant activity, and aroma quality. The α-L-rhamnosidase and the two enzymes combination reduced the naringin concentration from 356.33 to 10.52 and 4.17 mg/L, respectively. The α-L-rhamnosidase, β-D-glucosidase, and their combination significantly increased the antioxidant activity. The unpleasant cooking note was significantly weakened and the floral note was increased after three treatments. The combinational treatment increased the sweet note greatly. The substrate competition between α-L-rhamnosidase and β-D-glucosidase may help to explain the inconsistencies of the polyphenols, antioxidant activity, and aroma quality after single enzyme or combined enzymes treatments. The results indicated that taste, antioxidant activity, and aromatic quality were well improved by the combined treatment than either α-L-rhamnosidase or β-D-glucosidase treatment, providing an attractive reference for debittering orange juice. Practical applications The work showed that the combined treatment of α-L-rhamnosidase and β-D-glucosidase was better to remove the bitter taste of citrus juice than the solo treatment with α-L-rhamnosidase or β-D-glucosidase in regarding with taste, antioxidant activity, and aromatic quality. It helps to better the quality of citrus juice with enzymatic treatment.
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POLYMER INTERNATIONAL
·
2021
·Vol. 70
·期 12
·1717-1724
·
IF 2.99
Lei Wang,Yifan Huang,Binjie Xin,Tingxiao Li
Electrospun core–shell nanofibers have aroused attention in drug delivery systems and tissue engineering because of the latent structures which can encapsulate various drugs and obtain tailored release profiles. Here, poly( l -lactide- co -ε-caprolactone) (PLCL) with gelatin (GE) blends were employed to electrospin the shell of core–shell nanofibers which could act as a protection layer for the controlled released behavior of the model drug doxorubicin hydrochloride (DOX). Core–shell nanofiber membranes with different drug loaded amounts were fabricated and the corresponding drug release behaviors were researched. The surface morphology of the obtained nanofibers was assessed by SEM and the structure of the core–shell nanofibers were observed by inverted fluorescence microscopy and TEM. The hydrophilicity was evaluated by water contact angle testing and the results indicated that the obtained fibers all possess good hydrophilicity. The mechanical properties of the fiber membranes were evaluated by tensile testing. Exploring the composition of the fiber membranes by Fourier transform infrared spectroscopy proved that DOX existed in the drug-loaded fiber membranes. In vitro release was evaluated by UV–visible spectroscopy and the accumulative release curves of DOX showed that increasing drug rates would lead to increasing cumulative release amounts but decreasing cumulative drug release rates. The results indicated that drugs encapsulated in PLCL/GE core–shell nanofiber material would be efficient and a potential material for drug delivery systems. © 2021 Society of Chemical Industry
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NANO
·
2021
·
IF 1.556
Chengxiang Chu,Rui Huang,Xianfeng Wang,Yuanyi Deng,Tao Gu,Danqun Huo,Liang Zhang,Daidi Zhong,Changjun Hou
As an important amino acid in the body, L-histidine participates in various physiological processes. Therefore, it is of great necessity to establish a rapid and effective method for L-histidine sensing, especially in blood and urine. In this work, a fluorescence sensor for detection of L-histidine was successfully developed using Cu-MOFs/Fe 3 + sensor. Cu-MOFs with blue emission served as the identification unit and signal source, and Fe 3 + played the role of “quencher” in this system. Fe 3 + induced the fluorescence quenching of Cu-MOFs to form Cu-MOFs/Fe 3 + sensor due to the effect of photoinduced electron transfer (PET). After the addition of L-histidine, Fe 3 + was captured by L-histidine to form L-histidine-Fe 3 + complex, which inhibited the PET effect from Cu-MOFs to Fe 3 + , resulting in the fluorescence recovery of Cu-MOFs. With such a design, L-histidine detection was realized with a wide linear range from 1 μ M to 190 μ M. The limit of detection (LOD) was calculated as low as 0.156 μ M. In addition, successful detection of L-histidine in blood serum with high selectivity and sensitivity indicated the real applications and potential diagnostic value of Cu-MOFs/Fe 3 + system.
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FlatChem
·
2020
·
IF 0
Yang Xia,Kaiqian Wang,Ying Shi,Xingcai Gui,Chaonan Lv,Haisheng Tao
With the social and economic development, environmental pollution issues have gradually become a serious threat to ecosystem. Therefore, effective detection methods of environmental pollutants have been widely expanded. In this study, an electrochemical sensor based on a functioned graphene was constructed. The functional graphene was obtained by electrochemical deposition of L-cysteine on graphene oxide modified glassy carbon electrode. The composite on the modified glassy carbon electrode was characterized by scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The characterized results discovered that graphene oxide was reduced and cross-linked to L-Cys simultaneously, with a corrugated sheet structure. The electrochemical behavior of the electrode was investigated by electrochemical impedance spectroscopy and cyclic voltammetry, and proved that the electrode has a rapid transfer of electrons and a good catalytic effect on environmental pollutant of hydroquinone. The constructed electrode can be used to detect hydroquinone, with the detection limit of 1.5 μM (S/N = 3), and a good linear relationship in the range of 2–160 μM. The low relative standard deviation of identical sensors and good anti-interference for coexisting matters revealed that the sensor has an important theoretical significance and broad application prospect.
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ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Zhencheng Yu,Chuanshuang Hu,Litao Guan,Weiwei Zhang,Jin Gu
In this study, we reported an environmentally benign method for the synthesis of silver nanoparticles (AgNPs) by using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanofibrils (CNFs) as the only reducing agent and stabilizer under mild aqueous conditions. The AgNPs were tightly adhered to the CNF surface, and the resulting stable CNF–AgNP suspension can be used for rapid and selective detection of l-cysteine (Cys). The efficacy of the process relies upon the surface plasmon resonance properties of AgNPs and the aggregation effect of AgNPs in the presence of Cys. Under optimal ionic strength provided by NaCl, Cys interacted with AgNPs, leading to a change in yellow color of the CNF–AgNP to purple. The colloidal suspension showed high sensitivity (limit of detection of 49 nM) and rapid (5 min) and selective detection of Cys among 20 amino acids that make up human proteins, hence demonstrating great potential for analyzing practical samples.
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ANALYST
·
2020
·Vol. 146
·期 3
·956-963
·
IF 3.978
Jun Ma,Gege Feng,Ying Ying,Yong Shao,Yongxin She,Lufei Zheng,A. M. Abd EI-Aty,Jing Wang
Herein, an indirect SERS sensing assay was developed for the determination of glyphosate (Gly) in tap water. The mechanism of detection was based on relieving the inhibitory effect of L-cysteine (L-cys) on a Au–Pt nanozyme by combining Gly with L-cys through divalent copper ions (Cu2+). In this method, a novel nano-chain-like Au–Ag composite with good repeatability was successfully fabricated to detect SERS signals of oxTMB without disturbing TMB Raman signals. Under optimal conditions, the SERS signal intensity of oxTMB (at 1605 cm−1) was proportional to the concentration of Gly. The results showed a good linear response over the concentration ranges of 10 μg L−1 to 1000 mg L−1. The limit of detection and limit of quantitation of Gly were found to be 5 μg L−1 and 10 μg L−1, respectively. In addition, good anti-interference ability against interfering cations and structural analogues deserves to be mentioned. This SERS assay can be used for detection of Gly in tap water that can meet the needs of practical detection.
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高影响因子
POLYMER
·
2020
·
IF 4.231
Xu Yang,Baolong Wang,Di Sha,Yonggang Liu,Jiuduo Xu,Kai Shi,Cong Yu,Xiangling Ji
A series of injectable and antibacterial hydrogels based on ε-poly ( l -lysine)-modified poly (vinyl alcohol) (CPVA- g -EPL)/chitosan (CS)/Ag complexes was fabricated using oxidized dextran as a crosslinker. As-prepared hydrogels CPVA- g -EPL/CS exhibited swelling ratios of approximately 33.9–55.4 g g −1 and macroporous structure with average pore size of 54–102 μm in dry state. The concentration of bacteria ( Escherichia. coli and Staphylococcus. aureus ) after treating with hydrogels for 4 h decreased by four orders of magnitude compared with that after blank control treatment, indicating excellent antibacterial property. After adding Ag nanoparticles (AgNPs), the antibacterial activities of hydrogels (i.e., CPVA- g -EPL/CS/AgNPs) were further enhanced. Moreover, the prepared hydrogels possessed good biocompatibility against the mouse fibroblast. In a mouse skin defect model, histological examination showed increased thickness, integrity of the epidermal tissue and formation of the collagen fibers, implying that these hydrogels remarkably accelerated the wound healing process. Thus, this kind of injectable hydrogel can be a good candidate as wound healing dressings applied in wound therapy.
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