AAPS PHARMSCITECH
·
2019
·Vol. 20
·期 7
·1-10
·
IF 2.608
Xu Yingxin,Fang Tianxu,Yang Yifan,Sun Li’ang,Shen Qi
Docetaxel (DTX) was effective in the treatment of neoplasm but could only be administered intravenously with the poor oral bioavailability owing to its undesirable solubility, remarkably metabolic conversion, and other factors. Cimetidine (CMD), a classic CYP3A4 isozyme inhibitor, had exhibited a wide range of inhibition on the metabolism of many drugs. The aim of this study was to construct the novel docetaxel-cimetidine (DTX-CMD) complex and the chitosan-deoxycholate nanoparticles based on it to confirm whether this formulation could show advantages in terms of solubility, dissolution rate, small intestinal absorption, and oral bioavailability in comparison with the pure drug. The solid-state characterization was carried out by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FT-IR), and simultaneous DSC-TGA (SDT). Dissolution rate and kinetic solubility study were determined by evaluating the amount of DTX in distilled water and phosphate buffer solution (pH = 7.4), respectively. And small intestinal absorption and pharmacokinetics study were conducted in rats. The results of this study demonstrated that we successfully constructed DTX-CMD complex and its chitosan-deoxycholate nanoparticles. Furthermore, the DTX-CMD complex increased the solubility of DTX by 2.3-fold and 2.1-fold in distilled water and phosphate buffer solution, respectively. The ultimate accumulative amount of DTX-CMD complex nanoparticles through rat small intestinal in 2 h was approximately 4.9-fold and the oral bioavailability of the novel nanoparticles was enhanced 2.8-fold, compared with the pure DTX. The superior properties of the complex nanoparticles could both improve oral bioavailability and provide much more feasibility for other formulations of DTX.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2019
·Vol. 38
·期 1
·17-23
·
IF 2.804
Chi Wei-Wen,Zhang Rong-Yuan,Han Ting,Du Jian,Li Hong-Kun,Zhang Wei-Jie,Li Yong-Fang,Tang Ben Zhong
The copper-catalyzed and metal-free azide-alkyne click polymerizations have become efficient tools for polymer synthesis. However, the 1,3-dipolar polycycloadditions between internal alkynes and azides are rarely employed to construct functional polymers. Herein, the polycycloadditions of dibutynoate ( 1 ) and tetraphenylethene-containing diazides ( 2 ) were carried out at 100 °C for 12 h under solvent- and catalyst-free conditions, producing soluble poly(methyltriazolylcarboxylate)s (PMTCs) with high molecular weights in high yields. The resultant polymers were thermally stable with 5% weight loss temperatures up to 377 °C. The PMTCs showed aggregation-induced emission (AIE) properties. They could work as fluorescent sensors for detecting explosive with high sensitivity, and generate two-dimensional fluorescent photopatterns with high resolution. Furthermore, their triazolium salts could be utilized for cell-imaging applications.
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Tissue Engineering and Regenerative Medicine
·
2019
·Vol. 16
·期 6
·573-583
·
IF 2.272
Zhao Yang,Sun Qing,Wang Shurong,Huo Bo
Background: Mesenchymal stem cells (MSCs) have strong self-renewal ability and multiple differentiation potential. Some studies confirmed that spreading shape and area of single MSCs influence cell differentiation, but few studies focused on the effect of the circularity of cell shape on the osteogenic differentiation of MSCs with a confined area during osteogenic process. Methods: In the present study, MSCs were seeded on a micropatterned island with a spreading area lower than that of a freely spreading area. The patterns had circularities of 1.0 or 0.4, respectively, and areas of 314, 628, or 1256 μm 2 . After the cells were grown on a micropatterned surface for 1 or 3 days, cell apoptosis and F-actin were stained and analyzed. In addition, the expression of β-catenin and three osteogenic differentiation markers were immunofluorescently stained and analyzed, respectively. Results: Of these MSCs, the ones with star-like shapes and large areas promoted the expression of osteogenic differentiation markers and the survival of cells. The expression of F-actin and its cytosolic distribution or orientation also correlated with the spreading shape and area. When actin polymerization was inhibited by cytochalasin D, the shape-regulated differentiation and apoptosis of MSCs with the confined spreading area were abolished. Conclusion: This study demonstrated that a spreading shape of low circularity and a larger spreading area are beneficial to the survival and osteogenic differentiation of individual MSCs, which may be regulated through the cytosolic expression and distribution of F-actin.
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Food and Bioprocess Technology
·
2019
·Vol. 12
·期 10
·1741-1755
·
IF 3.032
Jiang Lei,Belwal Tarun,Huang Hao,Ge Zhiwei,Limwachiranon Jarukitt,Zhao Yechao,Li Li,Ren Guoping,Luo Zisheng
The extraction and characterization of bioactive compounds from bamboo shoot shell was performed under ultrasonic-assisted extraction (UAE) along with UPLC-Triple-TOF/MS analysis. Under optimized UAE conditions (ethanol concentration (58%), liquid to solid ratio (24 mL/g), extraction temperature (59 °C), and sonication time (29 min)), total phenolic content (TPC) was recorded highest as 85.3 mg GAE/g DW, while DPPH and FRAP antioxidant activities were 77 μmol AAE/g DW and 33 μmol AAE/g DW, respectively. Using UPLC-Triple-TOF/MS analysis, fifteen phenolic acids, seven flavonoids, nineteen organic acids, two iridoid glucosides, and one neoglinan were identified. Among them, p -coumaric acid (119 μg/g DW), chlorogenic acid (87 μg/g DW), rutin (39 μg/g DW), and ferulic acid (17 μg/g DW) were found to be the most abundant phenolic compounds. Furthermore, compounds including phenolic acids (3 -p- coumaroylquinic acid, cryptochlorogenic acid, 3 -O- feruloylquinic acid, 5 -O- feruloylquinic acid, 3 -O- caffeoylshikimic acid, 5 -p- coumaroylquinic acid, 1,3-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid), flavonoids (schaftoside/c-hexosyl-c-pentosylapigenin, apigenin 6,8-di-C-α- l -arabinopyranoside, kaempferide 3 -O- α- l -rhamnopyranosyl(1->6)-β- d -glucopyranoside, 6-C-β- d -glucopyranosyl-8-C-α- l -arabinopyranosylchrysin, narcissin), some organic acids, iridoid glucosides, and neolignan were also detected for the first time from bamboo shoot shell extract. The present study revealed that bamboo shoot shell extract contains a larger number of nutraceutically active compounds which can be further utilized by food and nutraceutical industries.
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JOURNAL OF ELECTRONIC MATERIALS
·
2019
·Vol. 48
·期 11
·7474-7478
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IF 1.676
Qing Zhenjun,Li Haiyan,Zhao Chenglin
A microwave dielectric BaCu 2 V 2 O 8 ceramic with a low intrinsic sintering temperature was synthesized by a solid-state sintering process. The influences of sintering process on the crystal phase composition, microstructure, relative density, and microwave dielectric properties of BaCu 2 V 2 O 8 ceramic were studied utilizing scanning electron microscopy and an x-ray diffractometer. The crystalline phase of BaCu 2 V 2 O 8 was the only one phase found in ceramic. The sample fired at 740°C for 6 h had the optimal microwave dielectric properties ( ε r = 13.5, Q × f = 46,300 GHz and τ f = − 41.5 ppm/°C). The results mean that electronic devices fabricated by low temperature co-fired ceramic technology based on BaCu 2 V 2 O 8 ceramic have potential applications in the high-frequency field.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 10
·1-9
·
IF 5.479
He Maofang,Wei Yinmao,Wang Rong,Wang Chunyang,Zhang Bo,Han Lu
A boronate-modified magnetic affinity sorbent was prepared by adopting hyperbranched polyethyleneimine as the scaffold to amplify initiator sites. 3-Acrylamidophenylboronic acid was employed as monomer to proceed in situ free-radical polymerization on magnetite (Fe 3 O 4 ) nanoparticles. Due to the improved density of boronic acid polymers, the adsorbent exhibited high adsorption capacity, typically (134 ± 8) μg mg −1 for dopamine, (92 ± 7) μg mg −1 for catechol, (363 ± 14) μg mg −1 for ovalbumin and (464 ± 22) μg mg −1 for horseradish peroxidase. These capacities are much higher than those of other adsorbents. The sorbent was applied to the enrichment of catecholamines from spiked human urine. Owing to the high adsorption capacity, only 1.0 mg of adsorbent was sufficient to eliminate the interferences and enrich the targets (dopamine, norepinephrine and epinephrine) within 5 min. They were quantified by HPLC. The recoveries from spiked samples range between 83.5% ~106%, with relative standard deviations of 3.2 ~ 9.4% ( n = 5). The separation of glycoproteins from egg white was also accomplished prior to their analysis by PAGE. In the authors’ perception, this approach is promising in that the density of functional groups on the adsorbent is strongly increased.
The preparation routine of boronate affinity magnetic adsorbent (Fe 3 O 4 @HpAAPBA). The adsorbent is used for the magnetic solid phase extraction of cis -dol compounds from real sample.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2019
·Vol. 30
·期 5
·1589-1599
·
IF 1.637
Zhao Peng,Zhang Jinteng,Jiang Jingjing,Wang Hongtao,Xie Tengfeng,Lin Yanhong
A novel Pt/CeO 2 /ZnO ternary composite is synthesized via two simple procedures of hydrothermal and photoreduction. The crystal structure, morphology, and composition of as-prepared samples are characterized by XRD, SEM, HRTEM, XPS and UV–Vis DRS. Because both ZnO and CeO 2 are good photocatalytic semiconductors, photocatalytic activities of the samples are evaluated by the degradation of phenol aqueous solution (25 mg/L). Under the strong interaction among the Pt, CeO 2 and ZnO, the maximum photocatalytic activity is observed in the Pt/CeO 2 /ZnO ternary composites and 91% phenol can be degraded in 60 min under UV light irradiation. The probable photocatalytic mechanism is discussed by active species trapping experiments along with SPV, TPV, PL and PA measurements. The enhanced photocatalytic activity is attributed to the redox cycle of Ce 4+ ↔ Ce 3+ , the effective interface between ZnO and CeO 2 as well as the electron transfer action of Pt nanoparticles. The photocatalytic activity almost unchanged after four cycles and proves excellent reusable photocatalysts. This work shows the synergistic effect of rare earth elements and noble metals in the photocatalytic process, which facilitates their practical application in toxic pollution abatement.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2020
·Vol. 36
·期 1
·120-126
·
IF 1.063
Chen Jianfei,Chen Xiaoyu,Zhang Xing,Yuan Yao,Bi Ruyi,You Feifei,Wang Zumin,Yu Ranbo
BiVO 4 , a promising visible-light responding photocatalyst, has aroused extensive research interest because of inexpensiveness and excellent chemical stability. However, its main drawback is the poor photoinduced charge-transfer dynamics. Building nanostructures is an effective way to tackle this problem. Herein, we put forward a new method to prepare nanostructured BiVO 4 from Bi-based metal-organic frameworks[Bi-MOF(CAU-17)] precursor. The as-prepared material has a rod-like morphology inherited from the Bi-MOF sacrificial template and consists of small nanoparticle as building blocks. Compared with its counterparts prepared by conventional methods, MOF-derived nanostructured BiVO 4 shows better light absorption ability, narrower bandgap, and improved electrical conductivity as well as reduced recombination. Consequently, BiVO 4 nanostructure demonstrates high photocatalytic activity under visible light towards the degradation of methylene blue. Methylene blue can be degraded up to 90% within 30 min with a reaction rate constant of 0.058 min −1 . Moreover, the cycling stability of the catalyst is excellent to withstand unchanged degradation efficiency for at least 5 cycles.
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Food Analytical Methods
·
2020
·Vol. 13
·期 3
·811-822
·
IF 2.667
Cheng Fuliang,Liao Xiaoning,Huang Zhiwen,Xu Liting,Zhou Ying,Zhang Xue
Thiabendazole (TBZ), a widely used agricultural antifungal, has been evidenced to pose serious threat to human health. However, highly sensitive detection of it in food samples is difficult owing to the complexity of food matrix. Herein, an electrochemical sensor, based on multiwall carbon nanotubes (MWCNTs) decorated two-dimensional layered molybdenum disulfide (MoS 2 ), was firstly developed for rapid detection of TBZ using square wave voltammetry method. It is demonstrated that the modification of MoS 2 with MWCNTs enhances the catalytic performance to TBZ oxidation. Under optimized conditions, the electrochemical sensor shows admirable linearity range, high selectivity and low detection limit. In pear, water spinach and tap water sample analyses, satisfactory recoveries from 92.83 to 101.92% with coefficients of variation less than 5% were obtained, showing high consistence with the HPLC results. This work puts forward a rapid and reliable method for the highly sensitive screening of the residues of TBZ in food resource.
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International Journal of Peptide Research and Therapeutics
·
2019
·Vol. 26
·期 4
·1991-1999
·
IF 1.219
Cao Mengjie,Lu Kui,Liu Guangbin,Zhao Dongxin,Qi Jianzhao
Affecting the repair function of RAD51 can effectively reverse tumor resistance to chemotherapy and radiotherapy. There is interaction between RAD51 181−200 and BRCA1 846−871 . Using the computer to simulate single mutation of lysine at 862 position of BRCA1 846−871 , six BRCA1 846−871 mutants with significant interaction with RAD51 181−200 were screened out. These mutant peptides were obtained by 9-fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) method. All peptides were purified and characterized by liquid chromatography and ESI-MS. In this study, the interaction between RAD51 181−200 and BRCA1 846−871 or mutant peptides was investigated using circular dichroism spectroscopy (CD) and fluorescence spectroscopy. The CD results shown that the secondary structure change of RAD51 181−200 induced by BRCA1 846−871 , BRCA1 846−871 K862F ( F ), BRCA1 846−871 K862G ( G ), BRCA1 846−871 K862E ( E ) and BRCA1 846−871 K862N ( N ) was obvious, which the α-helix content of RAD51 181−200 decreased from 58.3–36.1%, 58.3–36.2%, 58.3–36.4%, 58.3–36.5% and 58.3–36.0%, respectively. While, the addition of BRCA1 846−871 K862P ( P ) and BRCA1 846−871 K862Y ( Y ) hardly changed the α-helix content of RAD51 181−200 . From the fluorescence results, the binding constants of F and Y are 1.00 × 10 5 M −1 and 1.16 × 10 5 M −1 respectively, which were both larger than that of BRCA1 846−871 (9.01 × 10 2 M −1 ). The binding constant of RAD51 181−200 with E (7.45 × 10 3 M −1 ) is slightly higher than that of BRCA1 846−871 . The binding constants of RAD51 181−200 with P (1.24 × 10 3 M −1 ) and N (1.26 × 10 3 M −1 ) are similar to that of BRCA1 846−871 . However, the K b value of G (1.51 × 10 2 M −1 ) is less than that of BRCA1 846−871 . Comparing all spectral data, F is the most significant one interacting with RAD51 181−200 . These findings could provide direction for peptide processing and application.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-8
·
IF 4.011
Xu Haowen,Zhang Heyi,Ma Yuhui,Jiang Mao,Zhang Yewei,Wu Yinan,Zhang Haoran,Xia Ruidong,Niu Qiaoli,Li Xing’ao,Huang Wei
The morphology of perovskite light-absorption layer plays an important role in the performance of perovskite solar cells (PSCs). In this work, BiFeO 3 (BFO) nanostructures were used as additive for CH 3 NH 3 PbI 3 (MAPbI 3 ) via anti-solvent method. The addition of BFO nanostructures greatly enhanced the crystallinity, grain size and film uniformity of MAPbI 3 . As a result, the charge carrier mobility and electron diffusion length increased, leading to the increase of the short circuit current (J SC ) of PSCs. This work provides a very simple but effective approach to improve the morphology of perovskite layer for efficient PSCs.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-8
·
IF 5.479
Guo Ying,Zhang Juncai,Zhang Weihong,Hu Daodao
Brightly fluorescent carbon quantum dots coated with polyethylenimine (PEI-CDs) were prepared using malic acid and PEI as the precursors. The PEI-CDs have a high quantum yield (41%) and green emission (peaking at 502 nm under 430 nm excitation), both of which are not affected by high ionic strength. The PEI-CDs have a positive charge at physiological pH values and can electrostatically bind aptamers with their negative charge. This is shown for aptamers binding thrombin or ATP. Binding of aptamers results in quenching of fluorescence. If thrombin or ATP are introduced, the respective aptamer will bind them, and the complex is then released from the PEI-CDs. Fluorescence increases in proportion to the analyte concentration. Under optimized conditions, thrombin and ATP can be sensitively and selectively detected by fluorometry with lower detection limits of 1.2 and 13 nM, respectively. The assay was successfully applied to the determination of thrombin and of ATP in spiked serum samples.
Green fluorescent carbon quantum dots were functionalized with polyethyleneimine. They were applied to aptamer-based determination of thrombin and ATP. The PEI-functionalized carbon quantum dots (PEI-CDs) have bright green fluorescence are were synthesized by one-step hydrothermal treatment of malic acid and PEI. Employing the PEI-CDs, a fluorometric aptamer-based assay was developed for the determination of thrombin and ATP.
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