INDUSTRIAL CROPS AND PRODUCTS
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2016
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IF 3.449
Rui Li,Linlin Ding,Zhaoliang Wu,Yanji Wang,Wei Liu,Yanyan Wang
For reducing the costs of separating bromelain from its crude extract, a two-stage foam fractionation technology with β -cyclodextrin as an activity protector was developed using the foam fractionation column with vertical sieve trays (VSTs). By studying the effects of VSTs, volumetric air flowrate, β -cyclodextrin concentration, aeration time and temperature, the two-stage foam fractionation technology were optimized. The results show that VSTs effectively improved the activity recovery by enhancing the adsorption of bromelain at the gas-liquid interface while β -cyclodextrin significantly reduced the activity loss induced by the gas-liquid interface. At β -cyclodextrin concentration 0.60 mg/mL, a high specific activity of 165.6 U/mg, 7.4 folds of that in the crude extract, was obtained with an activity recovery of 45.2% by using the optimized two-stage foam fractionation technology. Compared to those without β -cyclodextrin, activity recovery and specific activity obtained satisfactory relative increases of 34.1% and 110.7%, respectively.
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ACS Catalysis
·
2016
·
IF 9.307
Nengchao Luo,Min Wang,Hongji Li,Jian Zhang,Huifang Liu,Feng Wang
One of the challenges of depolymerizing lignin to valuable aromatics lies in the selective cleavage of the abundant C–O bonds of β-O-4 linkages. Herein we report a photocatalytic oxidation–hydrogenolysis tandem method for cleaving C–O bonds of β-O-4 alcohols. The Pd/ZnIn2S4 catalyst is used in the aerobic oxidation of α-C–OH of β-O-4 alcohols to α-C═O with 455 nm light, and then a TiO2–NaOAc system is employed for cleaving C–O bonds neighboring the α-C═O bonds through a hydrogenolysis reaction by switching to 365 nm light. Interestingly, the oxidation–hydrogenolysis tandem reaction can be conducted in one pot to offer ketones and phenols (up to 90% selectivity) via a dual light wavelength switching (DLWS) strategy. EPR and metal loading experiments elucidate that Ti3+ in TiO2 is formed in situ and is responsible for the photocatalytic hydrogenolysis through electron transfer from Ti3+ to the β-O-4 ketones.
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TALANTA
·
2017
·
IF 4.162
Qiong Hu,Kefeng Ma,Yaqi Mei,Minhui He,Jinming Kong,Xueji Zhang
In this work, we report a novel use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the chromogenic Fe(BPDS) 3 4- (BPDS=bathophenanthroline disulfonic acid) reporter for the visual detection of β-galactosidase (β-Gal) activity and sandwich immunoassay . The enzymatic hydrolysis of the substrate p -aminophenyl-β-D-galactopyranoside can switch on the reduction of Fe 3+ to Fe 2+ and the subsequent complexation of Fe 2+ with the BPDS ligand to generate the Fe(BPDS) 3 4- reporter, leading to the appearance of the intense MLCT absorption band at 535 nm and the colorless-to-red color change of the solution. Simply through a single step, the activity of β-Gal can be sensitively and selectively detected within the dynamic range of 0−220 mU mL −1 , with a limit of detection (LOD) of 1.69 mU mL −1 . This approach is applicable for the visual detection of β-Gal activities in the presence of complex human serum samples. Besides, when integrated with the sandwich immunoassay of carcinoembryonic antigen, a LOD of 1.16 ng mL −1 can be achieved. In light of its prominent simplicity and practicality, our MLCT-based approach holds great potential in diagnostic and analytical applications.
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JOURNAL OF SEPARATION SCIENCE
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2017
·Vol. 40
·期 23
·4653-4660
·
IF 2.557
Haoran Fan,Jinpeng Wang,Qingran Meng,Yaoqi Tian,Xueming Xu,Zhengyu Jin
Photoirradiation surface molecularly imprinted polymers for the separation of 6- O -α- d -maltosyl-β-cyclodextrin were synthesized using functionalized silica as a matrix, 4-(phenyldiazenyl)phenol as a light-sensitive monomer, and 6- O -α- d -maltosyl-β-cyclodextrin as a template. Fourier transform infrared spectroscopy results indicated that 4-(phenyldiazenyl)phenol was grafted onto the surface of functionalized silica. The obtained imprinted polymers exhibited specific recognition toward 6- O -α- d -maltosyl-β-cyclodextrin. Equilibrium binding experiments showed that the photoirradiation surface molecularly imprinted polymers obtained the maximum adsorption amount of 6- O -α- d -maltosyl-β-cyclodextrin at 20.5 mg/g. In binding kinetic experiments, the adsorption reached saturation within 2 h with binding capacity of 72.8%. The experimental results showed that the adsorption capacity and selectivity of imprinted polymers were effective for the separation of 6- O -α- d -maltosyl-β-cyclodextrin, indicating that imprinted polymers could be used to isolate 6- O -α- d -maltosyl-β-cyclodextrin from a conversion mixture containing β-cyclodextrin and maltose. The results showed that the imprinted polymers prepared by this method were very promising for the selective separation of 6- O -α- d -maltosyl-β-cyclodextrin.
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BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Mai Luo,Yifan Hua,Yiran Liang,Jiajun Han,Donghui Liu,Wenting Zhao,Peng Wang
A novel functionalized carbon dot has been synthesized by covalently linking β-cyclodextrin to the surface of N, S codoped carbon dots (β-CD-CDs). The characterization was confirmed by transmission electron microscopy , X-ray photoelectron spectroscopy, infrared spectra, ultraviolet-visible, and fluorescence emission spectra. On the basis of this carbon dot and (ferrocenylmethyl) trimethylammonium iodide (Fc + ), a photo-induced electron transfer (PET) fluorescent probe system was developed to determine the concentration of testosterone in water and identify testosterone in cell by fluorescence imaging as a visible biomarker. Under the optimum condition, the fluorescent intensity of the probe system linearly responded to the concentration of testosterone from 0 μM to 280 μM and the limit of detection was 0.51 μM. This probe system also performed well at determining testosterone in groundwater with average recoveries of testosterone ranging from 96% to 107% at spiking levels of 0.5–100 μM, and the relative standard deviation remained below 13%, which provided a reliable, rapid and easy method to determine testosterone in environmental water. Furthermore, the low cytotoxicity, high anti-interference ability, and excellent biocompatibility of β-CD-CDs made this probe system successfully used in cell fluorescence imaging to monitor levels of testosterone in the cytoplasm of cells with a promising application value in medical research.
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ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
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2017
·Vol. 36
·期 8
·1991-1997
·
IF 2.951
XingChen Zhai,Hua Zhang,Min Zhang,Xin Yang,Cheng Gu,GuoPeng Zhou,HaiTian Zhao,ZhenYu Wang,AiJun Dong,Jing Wang
A rapid monitoring platform for sensitive voltammetric detection of thiamethoxam residues is reported in the present study. A β –cyclodextrin-reduced graphene oxide composite was used as a reinforcing material in electrochemical determination of thiamethoxam. Compared with bare glassy carbon electrodes, the reduction peak currents of thiamethoxam at reduced graphene oxide/glassy carbon electrode and β –cyclodextrin-reduced graphene oxide/glassy carbon electrode were increased by 70- and 124-fold, respectively. The experimental conditions influencing voltammetric determination of thiamethoxam, such as the amount of β –cyclodextrin-reduced graphene oxide, solution pH, temperature, and accumulation time, were optimized. The reduction mechanism and binding affinity of this material is also discussed. Under optimal conditions, the reduction peak currents increased linearly between 0.5 µM and 16 µM concentration of thiamethoxam. The limit of detection was 0.27 µM on the basis of a signal-to-noise ratio of 3. When the proposed method was applied to brown rice in a recovery test, the recoveries were between 92.20% and 113.75%. The results were in good concordance with the high-performance liquid chromatography method. The proposed method therefore provides a promising and effective platform for sensitive and rapid determination of thiamethoxam. Environ Toxicol Chem 2017;36:1991–1997. © 2017 SETAC
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2016
·
IF 2.567
Wan-Fen Pu,Yang Yang,Bing Wei,Cheng-Dong Yuan
In this work, a novel enhanced oil recovery (EOR) copolymer, which is expected to be an alternative to currently used polymers, was successfully synthesized through free radical micellar copolymerization of acrylamide, acrylic acid, 6-acrylamide-β-cyclodextrin (N-β-CD), and acrylamide-adamantane (N-ADA). The copolymer was characterized by Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance spectroscopy, thermal gravimetric analysis, and scanning electron microscopy. The results demonstrated that because of the incorporation of ADA and β-CD groups, host–guest interactions occurred in the aqueous solution, which subsequently improved the salt tolerance, temperature resistance, shear resistance, and viscoelastic properties of the synthesized polymer. Regarding the EOR performance, it was observed that 8.58% incremental oil was produced using this polymer after water flooding was exhausted. The superior properties make this novel polymer promising in enhancing oil recovery, particularly for reservoirs which have discarded polymer flooding techniques because of harsh conditions.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Yuting Fan,Luyu Gao,Jiang Yi,Yuzhu Zhang,Wallace Yokoyama
β-Carotene (BC), a naturally occurring lipophilic carotenoid, is beneficial for human health. However, its water solubility and bioavailability are low. In this study, organogel-based nanoemulsion was successfully prepared to improve the loading amount, solubility, and bioavailability of BC. Corn oil was selected as the oil phase for the organogel as a result of the greatest release amount of BC. Tween 20 was optimized as the emulsifier based on the highest extent of lipolysis and BC bioaccessibility. The nanoemulsion was a better alternative than the organogel according to both the extent of lipolysis and BC bioaccessibility. Cellular uptake of BC was significantly improved through organogel-based nanoemulsion compared to BC suspension. Caveolae-/lipid-raft-mediated route was the main endocytosis pathway. Pharmacokinetic results confirmed that the in vivo bioavailability of BC in nanoemulsion was 11.5-fold higher than that of BC oil. The information obtained suggested that organogel-based nanoemulsion may be an effective encapsulation system for delivery of insoluble and indigestible bioactive compounds.
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Food and Bioprocess Technology
·
2017
·Vol. 10
·期 10
·1844-1853
·
IF 2.576
Ye Youxin,Zhu Mingjin,Miao Keyan,Li Xinying,Li Defu,Mu Changdao
Antimicrobial activity is an attractive property for packaging materials which can extend the shelf life of products and provide microbial safety for consumers. The study aimed to analyze the physicochemical and antimicrobial properties of gelatin-based edible films containing trans -anethole as the active additive. Encapsulation with β-cyclodextrin was used as an effective way to introduce trans -anethole into gelatin matrix. The results showed that the trans -anethole/β-cyclodextrin inclusion complex could be evenly dispersed in the gelatin-based edible films with appropriate addition. The incorporation of trans -anethole conferred the edible films with good antimicrobial activity as expected, which increased with the content of trans -anethole increasing. Moreover, the addition of inclusion complex improved the tensile strength and surface hydrophobicity and reduced the moisture content of the edible films. It was interesting that the edible films presented great UV light barrier property and it was increased by the addition of inclusion complex. Overall, the antimicrobial gelatin-based edible films showed great potential as bioactive packaging materials to extend food shelf life.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Qin Liu,Zengbin Yin,Fuzhou Guo,Juntang Yuan
β-SiAlON ceramic tool materials were fabricated via spark plasma sintering using binary sintering additives (SmF 3 -Sm 2 O 3 ). The effects of SmF 3 addition and sintering temperature on the densification, phase composition, microstructure and mechanical properties were studied. Furthermore, the corrosion behavior of β-SiAlON ceramic tool materials due to excessive SmF 3 at high sintering temperatures (≥1700 °C) was revealed. The results indicated that SmF 3 could promote the densification and generation of β-SiAlON. Higher SmF 3 content and sintering temperature increased the aspect ratio and average grain size of β-SiAlON, which made fracture toughness increase and Vickers hardness decrease. The best density, fracture toughness and hardness of β-SiAlON ceramic tool materials were 3.204 g/cm 3 , 6.45 ± 0.07 MPa m 1/2 and 16.72 ± 0.09 GPa. However, β-SiAlON was corroded by excessive SmF 3 during high temperature sintering, resulting in deterioration in densification and properties.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaofei Ma,Wenyi Wu,Qin Wang,Jinqiu Xu,Liangliang Cai
This study integrated a chiral selector with micelle pseudostationary phase to develop a novel chiral separation method in capillary electrophoresis. A new type of nanomicelles materials was prepared via amidation reaction using the carboxymethyl-β-cyclodextrin (CM-β-CD) as the hydrophilic group and long chain fatty amines as the hydrophobic group. This novel amide compound self-assembled into nanomicelles in a buffer solution, acting as both a chiral selector and pseudostationary phase. The separations of model drugs were notably improved when compared to the single CM-β-CD system and CM-β-CD/sodium dodecyl sulfate micellar electrokinetic capillary chromatograph system. This study optimized several separation parameters and comprehensively characterized the nanomicelles through several methods such as infrared spectroscopy, nuclear magnetic resonance spectroscopy, particle size determination, and transmission electron microscopy (TEM). Additionally, amlodipine was chosen as the model analyte to determine drug loading in both the single CM-β-CD system and the nanomicelles system using high performance liquid chromatography. The findings indicated that nanomicelles exhibited superior drug loading capacity. The particle size and TEM analysis visually confirmed the distribution of enantiomers in the hydrophobic core of nanomicelles. The proposed nanomicelles showed promise in chiral separation and this strategy opened up new pathways for the development of functional chiral materials.
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FLAVOUR AND FRAGRANCE JOURNAL
·
2025
·
IF 2
Tanfang Xu,Junfeng Liu,Ping Wang,Junfeng Zan,Ke Yang,Daizhi Tian,Maohua Chen,Yanhua Li
Thymol (THY) is a natural antioxidant and antimicrobial; however, its biological activity and application in the food and pharmaceutical fields are limited due to its low water solubility and volatility. In this study, inclusion complexes of cationic-β-cyclodextrin-THY (C-β-CD-THY) were prepared by using the aqueous stirring method. Then UV–Vis spectra, FT-IR, NMR, DSC, XRD and SEM data were collected to characterise the structure and morphology of the inclusion complexes. Phase solubility experiments showed a better solubilisation effect of C-β-CD-THY than HP-β-CD-THY. At a concentration of 0.64 mmol/L for THY, the DPPH scavenging rate was 60.99% and 61.31% for HP-β-CD-THY and C-β-CD-THY, respectively, whereas that of THY in 10% ethanol was only 43.47%. The bacteriostatic activity was verified using a circle of inhibition and the live/dead bacterial double stain. C-β-CD-THY exhibited better antimicrobial activities against Escherichia coli and Staphylococcus aureus than HP-β-CD-THY. In conclusion, the C-β-CD-THY inclusion complexes have the potential to be applied as an effective bacteriostatic agent for food and pharmaceutical applications.
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