PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Feixiong Zheng,Zhangting Xu,Xiaoji Deng,Yiming Sun,Jaime A. Teixeira da Silva,Xiaoxia Shen,Zhenming Yu
Salt stress severely constrains plant growth and productivity, so understanding the underlying mechanisms of salt stress is crucial for the genetic improvement of plants. Aquaporins (AQPs), which facilitate the transport of water or nutrients, exert an indispensable role in plants' response to salt stress. The AQP family in Dendrobium officinale , a commercially important medicinal and edible orchid, has not been identified or functionally characterized, while an understanding of this plant's tolerance to salinity remains unexplored. In this study, a genome-wide identification of AQP genes in D. officinale was performed using a salt-induced transcriptome. The 28 AQP members were mined and classified into four subfamilies (NIPs, PIPs, SIPs, and TIPs), and their physicochemical and bioinformatic properties were analyzed. Overexpression of DoTIP1-1 in D. officinale significantly enhanced salt tolerance via the accumulation of proline and water-soluble polysaccharides that improved water retention. Furthermore, salt tolerance was fortified by the improved activity of antioxidant enzymes (peroxidase and superoxide dismutase), allowing reactive oxygen species to be scavenged, and augmenting photosynthetic efficiency due to the upregulated expression of stress-responsive genes. This study provides the first comprehensive analysis of the AQP family in D. officinale and demonstrates that DoTIP1-1 is a key genetic determinant for enhancing salt tolerance, offering significant potential for the genetic improvement of this plant to resist abiotic stress.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Qingling Meng,Mingjiang Mao,Chenhuan Shentu,Shijie Dai,Hao Qian,Jie Hao,Buyang Chen,Bin Ding,Zhiqiang Zhang,Xiaofeng Yuan
Atractylodes macrocephala is a traditional Chinese medicinal (TCM) herb widely used for treating gastrointestinal disorders, yet the structural features and mechanisms of its polysaccharides remain insufficiently understood. In this study, we isolated and characterized a neutral glucan ( A. macrocephala polysaccharide 1, AMP1) from the rhizome of A. macrocephala , with an average molecular weight of 7.98 kDa and a monosaccharide composition of glucose (Glc) and arabinose (Ara) in a molar ratio of approximately 129: 1. Structural analyses revealed that AMP1 is a glucan with a backbone composed of →4)-α-Glc p -(1→ residues and three types of branching units, namely →4)-α-Glc p -(1→, →6)-α-Glc p -(1→, and →6)-β-Glc p -(1→. Notably, the →6)-β-Glc p -(1→ branches appear to carry an additional substitution at the C-4 position. Functional assays demonstrated that AMP1 suppressed the release of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, in LPS-stimulated macrophages. In vivo, AMP1 markedly ameliorated DSS-induced colitis in mice, as evidenced by a lower disease activity index (DAI), restoration of epithelial barrier integrity, and modulation of gut microbiota composition. Metabolomic profiling further revealed enrichment of beneficial microbial metabolites, implicating coordinated regulation of host–microbe metabolic pathways. Collectively, these findings establish AMP1 as a structurally defined polysaccharide with multifaceted protective effects, highlighting its therapeutic potential against ulcerative colitis (UC).
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RUSSIAN JOURNAL OF GENERAL CHEMISTRY
·
2017
·Vol. 86
·期 12
·2744-2751
·
IF 0.553
Ying S.,Wang Jia,Xu C.,Kang Y.,Zhang X.,Shi L.,Fan L.,Wang Z.,Zhou J.,Wu X.,Wu J.,Li W.,Liang G.
Fibroblast growth factor receptor 1 (FGFR1) is considered a therapeutic target for multiple cancers, including gastric cancer. FGFR1 inhibitors, being ATP competitors, can prevent the kinase domain and the downstream signaling cascade from phosphorylation and thus have the potential to treat cancers associated with aberrant FGFR1 activation. However, untargeted inhibition may cause numerous side effects. Thus, a non-ATP competitive FGFR1 inhibitor should be urgently identified and explored. In this study, we designed and synthesized 17 derivatives of nordihydroguaiaretic acid (NDGA), a known ATP-independent FGFR3 inhibitor. In the kinase activity assay, 3,5-bis(2-fluorobenzylidene)piperidin-4-one ( 1B ) showed the highest kinase inhibitory activity among all derivatives and was thus identified as a non-ATP-competitive FGFR1 inhibitor. In the biological effect evaluation, 1B restrained the FGFR−FRS2−ERK signaling pathway in a dose-dependent manner and inhibited the growth of two gastric cancer cell lines. Overall, 1B can be considered as a potential candidate for treating gastric cancer and as an outstanding lead compound for the discovery of novel non-ATPcompetitive FGFR1 inhibitors.
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Cell Death & Disease
·
2018
·Vol. 9
·期 6
·1-14
·
IF 5.638
Tao Liang,Lin Hongfa,Wen Jingjing,Sun Qi,Gao Yan,Xu Xi,Wang Junsong,Zhang Jianfa,Weng Dan
Endoplasmic reticulum (ER) stress contributes to the development and progression of many chronic inflammatory diseases, including type 2 diabetes, obesity, atherosclerosis, neurodegenerative diseases, and cancer. ER stress has been reported to induce inflammasome activation and release of mature IL-1β, which contributes to many inflammatory diseases. The molecular mechanisms that activate the inflammasome during ER stress are still poorly understood. Here we report that the kinase receptor-interacting protein 1 (RIP1) plays an important role in ER stress-induced activation of inflammasome. Inhibition of RIP1 kinase activity by Necrostatin-1 or siRNA-mediated RIP1 knockdown significantly reduced ER stress-induced caspase-1 cleavage and IL-1β secretion in both bone marrow-derived macrophages (BMDMs) and J774A.1 macrophages. We speculate that the mitochondria fission factor dynamin-related protein 1 (DRP1) and reactive oxygen species (ROS) might function as the effectors downstream of RIP1 to mediate inflammasome activation. Our study reveals a critical role for RIP1 in regulating ER stress-induced inflammation responses, and proposes RIP1 as a potential pharmaceutical target to treat diseases resulting from unresolved ER stress-related inflammation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenyu Luo,Binhua Huang,Minzhen Lei,Zhijian Wu,Qian Yu,Dawei Zhang,Chunyan Yan
Throughout the history of traditional Chinese medicine, Angelica pubescens has been extensively employed to treat inflammatory arthritis. However, little is known about the anti-rheumatoid arthritis (RA) effects of A. pubescens polysaccharides. In this study, four crude polysaccharides (APR50, APR70, APR90, and APRB) were isolated from A. pubescens radix, and the rat model of collagen-induced arthritis (CIA) was established to evaluate the anti-RA effects of A. pubescens polysaccharides. A. pubescens polysaccharides could effectively reduce the degree of paw swelling in CIA rats, among which the effect of APR50 was comparable to that of methotrexate. A homogenous polysaccharide APRP50-2-1 was purified from APR50. Structural characterization indicated that APRP50-2-1 was primarily composed of glucose, mannose, galactose, and arabinose. The main skeleton of APRP50-2-1 consisted of →4)- α - d -Glc p -(1→, →6)- α - d -Glc p -(1→, and →6,4)- α - d -Gal p -(1→ residues. The branched chains of →4)- α - d -Glc p -(1→ and →3)- β - d -Man p -(1→ were substituted at O -4 of →4,6)- α - d -Gal p -(1→. APRP50-2-1 inhibited the proliferation and promoted the apoptosis of MH7A cells by down-regulating the expression of the anti-apoptotic protein B-cell lymphoma-2 (BCL-2) and up-regulating the expression of the pro-apoptotic protein BCL2-associated X (BAX). In conclusion, A. pubescens polysaccharides had significant anti-RA effects in rat CIA model, among which APR50 and APRP50-2-1 possessed the promising prospects of ameliorating RA.
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NEUROCHEMISTRY INTERNATIONAL
·
2025
·
IF 4
Yinpei Huang,Hailun Xie,Lian Liu,Heng Zhao,Bing Li,Feng Zhang
Obstructive sleep apnea (OSA) is characterized by chronic intermittent hypoxia (IH), which induces oxidative stress and neuronal apoptosis, ultimately leading to progressive cognitive impairment. This study investigated the neuroprotective potential of a reactive oxygen species (ROS)-responsive nanotherapeutic, namely TPCD nanoparticles (TPCD NP), synthesized through the conjugation of Tempol and phenylboronic acid pinacol ester to β-cyclodextrin, in both in vivo and in vitro models. In rats, intravenous administration of TPCD NP improved memory performance as assessed by the Morris water maze test, and preserved hippocampal neuronal morphology. TPCD NP significantly reduced intracellular ROS content and malondialdehyde (MDA) levels while restoring antioxidant capacity, including superoxide dismutase (SOD) and glutathione (GSH). Apoptosis was attenuated, as evidenced by the downregulation of Bax and cleaved caspase-3, and the upregulation of Bcl-2 expression. Mechanistically, TPCD NP enhanced the nuclear translocation of nuclear factor erythroid 2–related factor 2 (NRF2), suppressed Kelch-like ECH-associated protein 1 (KEAP1), and increased heme oxygenase-1 (HO-1) expression. The protective effects were abolished by ML385, a selective NRF2 inhibitor, confirming the essential role of NRF2 activation in mediating the antioxidant and anti-apoptotic effects of TPCD NP. In conclusion, TPCD NP attenuates oxidative stress and apoptosis induced by IH in the hippocampus by activating the NRF2/KEAP1/HO-1 pathway. These findings highlight TPCD NP as a promising therapeutic strategy for OSA-associated neurodegeneration.
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JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2025
·
IF 2.5
Binbin Lu,Qian Zhang,Hang Lin,Shaowei Wang,Yang Zhu,Ren-shan Ge,Xingwang Li
We investigated the inhibitory effects of dithiocarbamates (DTCs) on human steroid 5α-reductase 1 (SRD5A1), an enzyme crucial for the conversion of testosterone or pregnenolone into neuroactive steroids. Utilizing a comprehensive approach that included enzyme assays, molecular docking simulations, and both structure-activity relationship (SAR) and 3D quantitative SAR (3D-QSAR) analyses, we assessed the potency and interaction mechanisms of DTCs with SRD5A1 in human SF126 cells. Our results demonstrated that zinc dibutyldithiocarbamate, disulfiram, ferbam, thiram, and ziram displayed significant inhibitory activity against SRD5A1, with IC 50 values of 1.10, 1.62, 2.31, 1.74, and 1.84 μM, respectively. Kinetic studies indicated that these compounds function as mixed inhibitors, suggesting a multifaceted interaction with the enzyme's active site. These DTCs effectively penetrated the cell membrane of SF126 cells to inhibit SRD5A1 at ≥ 5 μM. Molecular docking analyses revealed that these compounds interacted with a distinct domain located between the NADPH and testosterone binding sites of SRD5A1, primarily through sulfur bonds, which are a fundamental component of the DTC structure. Experimental validation of the inhibitory mechanism was achieved by demonstrating that the co-incubation of dithiothreitol, a sulfhydryl-reducing agent, significantly reversed the inhibitory effects of zinc dibutyldithiocarbamate and thiram. SAR analysis revealed a negative correlation between LogP, molecular weight, and IC 50 values, and a positive correlation between LogS, lowest binding energy, and IC 50 values. The 3D-QSAR analysis further indicated that hydrogen bond acceptor and hydrophobic region capabilities significantly contribute to the primary inhibition. In conclusion, this research significantly advances our understanding of the SAR of sulfur-containing DTCs as inhibitors of human SRD5A1, providing valuable insights for identifying toxicity of DTCs targeting this enzyme.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Ganggang Guo,Qiong Xi,Tao Yin,Jianju Nie,Yayuan Zhang,Li Guan,Zhenyang Liu,Fenghe Wang,Xu Li
Broadband near-infrared (NIR) phosphors are key materials for the preparation of NIR phosphor-converted light-emitting diodes (pc-LEDs). The wider coverage of the NIR spectrum means that more substances can be detected by NIR light and more applications are possible. However, ultra-broadband NIR phosphors with the full-width at half maximum (FWHM) greater than 200 nm still face challenges in terms of thermal stability as well as internal quantum efficiency (IQE). In this work, a series of La 2 CaTa x Zr (1- x ) O 6 (LCTZO): Cr 3+ phosphors was successfully synthesized by the high-temperature solid-phase method. The non-equivalent substitution strategy of Ta 5+ in place of Zr 4+ effectively modulates the crystal field environment of Cr 3+ and reduces the number of defects, thus modulating the luminescence properties of the phosphor. Under the 471 nm excitation, the spectrum of La 2 CaTa 0.3 Zr 0.7 O 6 : 2 % Cr 3+ can cover the 700–1400 nm band (FWHM = 201 nm) with IQE of 42.6 %, and the emission intensity can be maintained at 373 K at 74 % of that at room temperature . The prepared NIR pc-LED with ultra-wide spectral coverage shows better potential applications in the fields of night vision and biological tissue penetration.
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高影响因子
ELECTROCHIMICA ACTA
·
2012
·
IF 3.832
Yao Huang,Baoyang Lu,Jingkun Xu,Ge Zhang,Leiqiang Qin,Shuai Chen,Fangfang Kong
Anionic ring-open polymerization of ethylene oxide (EO) functionalized 1-hydroxy-2-methoxybenzene (HMOB) monomer 1-(2,3-epoxypropyl)-2-methoxybenzene (EPMOB) led to the formation of the precursor polymer oligo(1-(2,3-epoxypropyl)-2-methoxybenzene) (OEPMOB). Poly(OEPMOB- co -1-hydroxy-2-methoxybenzene) films P1 , P2 , P3 , and P4 were successfully achieved by secondary electrochemical copolymerization of the monomer mixtures OEPMOB/HMOB with different mass ratios of 3:1, 2:1, 1:1, and 1:2, respectively. The possibility of inter- and intramolecular polymerization resulted in a cross-linking conjugated network microstructure. A series of experiments were carried out to investigate the influence of monomer mass ratios on the electrochemical copolymerization and other properties of copolymer films. UV–vis, FT-IR, and 1 H NMR were used to characterize the structure of copolymers. Fluorescence spectroscopic studies revealed that the copolymers partly dissolved in DMSO were all good blue light-emitters, with strong emissions at 392 nm in the doped state. Since EO and HMOB units were introduced into the skeletal backbone, thermal stability of copolymers was improved in comparison with their corresponding homopolymers.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·Vol. 26
·期 16
·12530-12536
·
IF 2.9
Dingding Lv,Xinlei Chen,Ningjing Jiang,Guanjun Wang,Xiaoqing Zeng,Wei Fang,Weixing Li,Mingfei Zhou
Trifluoroacetylacetone (TFAA) has two enol forms, which can switch to each other via proton transfer. While much attention has been paid to their conformational preferences, the influence of microsolvation on regulating the proton position remains unexplored. Herein, we report the rotational spectra of trifluoroacetylacetone–(water)n (n = 1–3) investigated by chirped pulse Fourier transform microwave spectroscopy in the 2–8 GHz frequency range. Two conformers were identified for both TFAA–H2O and TFAA–(H2O)2, while only one conformer was characterized for TFAA–(H2O)3. The results indicate that water binding on the CH3 side stabilizes the enolF form, whereas water binding on the CF3 side stabilizes the enolH form. The enolF form predominates over the enolH form in these hydrated complexes, which contrasts with the fact that only enolH exists in isolated TFAA. EnolH becomes preferred only when water inserts itself into the intramolecular hydrogen bond. Instanton theory calculations reveal that the proton transfer reaction is dominated by quantum tunneling at low temperatures, leading to the stable existence of only one enol form in each configuration of the hydrated clusters.
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CERAMICS INTERNATIONAL
·
2018
·Vol. 44
·期 12
·14638-14644
·
IF 3.057
Shufang Si,Hongmei Deng,Wenliang Zhou,Tiantian Wang,Pingxiong Yang,Junhao Chu
In the process of exploring ideal ferroelectric perovskites for photovoltaic applications, a novel kind of ceramic (1- x )KNbO 3 - x BaCo 1/3 Nb 2/3 O 3 ( x KBCNO) has been synthesized using solid-state method. The phase transition, microstructure, optical and ferroelectric properties of the compounds were investigated in detail. X-ray diffraction and Raman scattering spectra analyses indicate that single phase ceramics have been formed with cubic perovskite structure in space group Pm 3 m . Obvious crystal lattice expansion can be found as x increases. A narrow band gap of 1.87 eV is observed at x = 0.75, which proves the dramatic effect of BaCo 1/3 Nb 2/3 O 3 (BCNO) addition on the energy band gap . Besides, the room-temperature ferroelectricity has been detected in KBCNO ceramics controlled by the BCNO doping content. These results lay a solid foundation on further exploring the phase transition, band gap engineering, and electric properties in perovskite oxides , and indicate potential applications in perovskite solar cells .
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2022
·Vol. 119
·期 51
·e2211193119
·
IF 12.779
Jieming Sheng,Le Wang,Andrea Candini,Wenrui Jiang,Lianglong Huang,Bin Xi,Jize Zhao,Han Ge,Nan Zhao,Ying Fu,Jun Ren,Jiong Yang,Ping Miao,Xin Tong,Dapeng Yu,Shanmin Wang,Qihang Liu,Maiko Kofu,Richard Mole,Giorgio Biasiol,Dehong Yu,Igor A. Zaliznyak,Jia-Wei Mei,Liusuo Wu
An interplay of geometrical frustration and strong quantum fluctuations in a spin-1/2 triangular-lattice antiferromagnet (TAF) can lead to exotic quantum states. Here, we report the neutron-scattering, magnetization, specific heat, and magnetocaloric studies of the recently discovered spin-1/2 TAF Na2BaCo(PO4)2, which can be described by a spin-1/2 easy axis XXZ model. The zero-field neutron diffraction experiment reveals an incommensurate antiferromagnetic ground state with a significantly reduced ordered moment of about 0.54(2) μB/Co. Different magnetic phase diagrams with magnetic fields in the ab plane and along the easy c-axis were extracted based on the magnetic susceptibility, specific heat, and elastic neutron-scattering results. In addition, two-dimensional (2D) spin dispersion in the triangular plane was observed in the high-field polarized state, and microscopic exchange parameters of the spin Hamiltonian have been determined through the linear spin wave theory. Consistently, quantum critical behaviors with the universality class of d = 2 and νz = 1 were established in the vicinity of the saturation field, where a Bose–Einstein condensation (BEC) of diluted magnons occurs. The newly discovered quantum criticality and fractional magnetization phase in this ideal spin-1/2 TAF present exciting opportunities for exploring exotic quantum phenomena.
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