Bioengineered
·
2022
·
IF 6.832
Zhipeng Jiang,Zehui Hou,Wei Liu,Zhuomin Yu,Zhiqiang Liang,Shuang Chen
The dysregulated circular RNAs (circRNAs) are linked to progression and chemoresistance in colorectal cancer (CRC). However, the role of circRNA protein tyrosine kinase 2 (circPTK2) in CRC progression and chemoresistance is uncertain. The circPTK2, microRNA (miR)-136-5p, m6A ‘reader’ protein YTH domain family protein 1 (YTHDF1), β-catenin and cyclin D1 abundances were examined via quantitative reverse transcription PCR or Western blotting. The progression was investigated by cell counting kit-8 (CCK-8), colony formation, transwell and xenograft analysis. The resistance to 5-fluorouracil (5-FU) and oxaliplatin was analyzed via detecting cell viability and apoptosis using CCK-8 analysis and flow cytometry. The binding relationship was examined through dual-luciferase reporter, RNA immunoprecipitation and pull-down analysis. In our study, circPTK2 abundance was enhanced in CRC and associated with liver metastasis, clinical stage and chemoresistance. CircPTK2 knockdown constrained cell proliferation, migration, invasion, resistance to 5-FU and oxaliplatin, and the Wnt/β-catenin signaling. MiR-136-5p was bound with circPTK2 and downregulated in CRC. MiR-136-5p knockdown attenuated the influence of circPTK2 silence on CRC progression and chemoresistance. YTHDF1 was targeted via miR-136-5p and upregulated in CRC samples and cells. MiR-136-5p targeted YTHDF1 to restrain CRC progression and chemoresistance. In addition, we confirmed that circPTK2 silence reduced xenograft tumor growth. In conclusion, circPTK2 interference suppressed CRC proliferation, migration, invasion and chemoresistance via regulating miR-136-5p and YTHDF1.Abbreviations: circRNAs: circular RNAs; CRC: colorectal cancer; circPTK2: circRNA protein tyrosine kinase 2; miR: microRNA; YTHDF1: YTH domain family protein 1; CCK-8: cell counting kit-8; 5-FU: 5-fluorouracil; RIP: RNA immunoprecipitation.
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Analytical Methods
·
2023
·Vol. 15
·期 27
·3318-3324
·
IF 3.1
Hongyu Chen,Mingshuo Liu,Jianing Wang,Yunfei Wang,Yajuan Wang,Wenyan Liu
Due to their excellent optical and electrical properties, all-inorganic metal halide perovskite CsPbBr3 quantum dots (QDs) have become one of the most promising materials in the field of optoelectronics during recent years. However, the stability of CsPbBr3 QDs limits their practical application and further development to a certain extent. In order to improve their stability, CsPbBr3 QDs were modified with 2-n-octyl-1-dodecanol for the first time in this paper. The 2-n-octyl-1-dodecanol-modified CsPbBr3 QDs were prepared by the ligand-assisted reprecipitation (LARP) method at room temperature in an air environment. Then the stability of the samples was tested at different temperatures and humidity. When the humidity was 80%, the photoluminescence (PL) intensity of both unmodified and modified CsPbBr3 QDs increased to different degrees because the appropriate amount of water changed the crystallization environment. The PL intensity of the modified QDs increased, and the peak positions were basically not shifted, proving that they did not agglomerate. Thermal stability test results showed that the PL intensity of the 2-n-octyl-1-dodecanol-modified QDs could still maintain 65% of the original intensity at 90 °C, which is 4.6 times that of the unmodified CsPbBr3 QDs. Experimental results show that the stability of CsPbBr3 QDs is significantly improved after 2-n-octyl-1-dodecanol modification, which demonstrates the excellent surface passivation of CsPbBr3 QDs by 2-n-octyl-1-dodecanol.
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ACS Catalysis
·
2023
·
IF 12.9
Zhen Chen,Haixin Chen,Kun Wang,Jian Chen,Ming Li,Yi Wang,Panagiotis Tsiakaras,Shuqin Song
The photocatalytic oxygen reduction reaction (ORR) is a promising strategy that generates H2O2 with a lower energy input. Although great progress has been made in the development of photocatalysts, poor accessibility of O2 in photocatalysts interfacial microenvironment is the rate-limiting factor for the photocatalytic 2e– ORR and still little explored. Here, we design a simple method to regulate the hydrophobicity and O2 accessibility at a controllable crystal facet ratio of TiO2 photocatalyst by a facile stearic acid (SA) modification. The adsorption structure of SA bonding with TiO2 has been investigated in detail by Raman spectra, X-ray photoelectron spectroscopy, Fourier transform infrared spectra, and density functional theory calculations. It has been found that the stable ester bond is formed between SA molecules and the TiO2 surface, thus leading to the enhanced hydrophobicity with the increase of SA loading. The highest photocatalytic H2O2 production rate from 2e− ORR on SA-modified TiO2 can reach up to 3160 μM h–1 g–1, which is 1.69 times that of the corresponding pure TiO2 sample. From the results of electrochemical impedance spectroscopy and molecular dynamics simulation, the increase of the O2 concentration in the interfacial microenvironment benefiting from the SA modification is responsible for the enhancement of 2e– ORR activity rather than the increase of hydrophobicity.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yidan Wang,Yunlei Xianyu
The coordination chemistry between phosphorylated molecules and metal ions has been reported, while few studies focus on its sensing capability. Herein, we report a colorimetric sensing strategy through the coordination chemistry between ascorbic acid 2-phosphate (AAP) and copper ions. The phosphate group-containing AAP can coordinate with copper ions to induce a visible color change from blue to green in a rapid way, which can be easily read by the naked eye or a smartphone based on the blue-to-green (B/G) ratio. This coordination chemistry provides a facile and convenient strategy for designing colorimetric assays. Alkaline phosphatase can catalyze the hydrolysis of AAP to ascorbic acid (AA), thus modulating the AAP/AA transformation and the AAP-mediated coordination, offering a straightforward way for monitoring the enzymatic activity. This colorimetric sensing strategy shows good performances in stability, sensitivity, cost, and scale-up production, holding great promise as a point-of-care technique for diagnostic applications.
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MICROBIAL PATHOGENESIS
·
2023
·
IF 3.8
Jing Zuo,Yamin Shen,Haikun Wang,Shuji Gao,Shuo Yuan,Dong Song,Yuxin Wang,Yang Wang
Streptococcus suis ( S. suis ) regulates biofilm formation through LuxS/AI-2 quorum sensing system, increasing drug resistance and exacerbating infection. The anti-hyperglycaemic agent metformin has anti-bacterial and anti-biofilm activities. This study aimed to investigate the anti-biofilm and anti-quorum sensing activity of metformin in S. suis . We first determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of metformin on S. suis . The results indicated that metformin showed no obvious inhibitory or bactericidal effect . Crystal violet staining showed that metformin significantly inhibited the formation of S. suis biofilm at sub-MIC concentration, which was also confirmed by scanning electron microscopy. Then, we quantified the AI-2 signal molecules in S. suis , and the results showed that metformin had a significant inhibitory effect on the production of AI-2 signal in S. suis . Inhibition of enzyme activity and molecular docking experiments showed that metformin has a significant binding activity to LuxS protein. In addition, qRT-PCR results showed that metformin significantly down-regulated the expression of AI-2 synthesis-related genes luxS and pfs , and adhesion-related genes luxS , pfs , gapdh , sly , fbps, and ef . Western blotting also showed that metformin significantly reduced the expression of LuxS protein. Our study suggests that metformin seems to be a suitable candidate for the inhibition of S. suis LuxS/AI-2 QS system and prevention of biofilm formation, which provided a new idea for the prevention and control of S. suis .
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TALANTA
·
2023
·
IF 6.1
Ying Li,Jian Xue,Rongjun Yu,Siling Chen,Xiangyu Deng,Anyi Chen,Jingfu Qiu
Herein, a ternary PdPtRu nanodendrite as novel trimetallic nanozyme was reported, which possessed excellent peroxidase-like activity as well as electro-catalytic activity on account of the synergistic effect between the three metals. Based on the excellent electro-catalytic activity of trimetallic PdPtRu nanozyme toward the reduction of H 2 O 2 , the trimetallic nanozyme was applied to construct a brief electrochemical immunosensor for SARS-COV-2 antigen detection. Concretely, trimetallic PdPtRu nanodendrite was used to modify electrode surface, which not only generated high reduction current of H 2 O 2 for signal amplification, but also provided massive active sites for capture antibody (Ab 1 ) immobilization to construct immunosensor. In the presence of target SARS-COV-2 antigen, SiO 2 nanosphere labeled detection antibody (Ab 2 ) composites were introduced on the electrode surface according sandwich immuno-reaction. Due to the inhibitory effect of SiO 2 nanosphere on the current signal, the current signal was decreased with the increasing target SARS-COV-2 antigen concentration. As a result, the proposed electrochemical immunosensor presented sensitive detection of SARS-COV-2 antigen with linear range from 1.0 pg/mL to 1.0 μg/mL and limit of detection down to 51.74 fg/mL. The proposed immunosensor provide a brief but sensitive antigen detection tool for rapid diagnosis of COVID-19.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 5
·1-10
·
IF 5.7
Ni Yun,Chen Xiu,Ling Chen,Zhu Ziye,Yue Yao,Wang Jie,He Aolin,Liu Ruijiang
The CYP2C19*2 gene carriers and non-carriers are closely related to the dosage of clopidogrel. To correctly guide the use of clopidogrel and promote individualized therapy, an ultra-sensitive electrochemical biosensor was developed for the detection of CYP2C19*2 gene. The heterogeneous α-Fe 2 O 3 /Fe 3 O 4 nanosheets were prepared via the hydrothermal-calcination process, and the preparation parameters were optimized. The average diameter and thickness of the nanosheets were approximately 150 nm and 53 nm, respectively; and the saturation magnetization was 80.2 emu/g. The α-Fe 2 O 3 /Fe 3 O 4 @Au nanosheets were prepared by sodium borohydride reduction method, and self-assembled to the electrode surface with magnetic field. Ultra-sensitive detection of CYP2C19*2 gene was realized through the recognition ability of strong single base mismatching of peptide nucleic acid and signal amplification effect of magnetic α-Fe 2 O 3 /Fe 3 O 4 @Au nanosheets. Under optimal detection conditions, the current had a good linear correlation with the negative logarithm of CYP2C19*2 gene concentration in the range 1 pM—1 nM, and the detection limit was 0.64 pM (S/N = 3). Meanwhile, the electrochemical signals of target DNA and incomplete complementary DNA were detected. The constructed biosensor exhibited good selectivity, reproducibility, and stability, providing a promising strategy for the detection of other gene mutations by electrochemical biosensors. Graphical Abstract
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PLoS One
·
2023
·Vol. 18
·期 4
·e0281189
·
IF 3.7
Yanzhuo Yang,Qingting Yu,Bin Li,Zuisu Yang,Shuai Zhang,Falei Yuan
Palmitic acid (PA) is considered a major contributor to the inflammation in many metabolic diseases; however, this role has been questioned recently for the complicated procedures in preparing PA-bovine serum albumin (BSA) complex. This study is aimed to evaluate the effect of PA-BSA complexing methods on cell viability and inflammatory responses of BV-2 cells. Three commercially available BSA brands and two types of solvents were compared for their effects on the expression of inflammatory cytokines. Three commonly used proportions of PA-BSA were tested for cell viability and inflammatory responses. We found that all the three types of BSA were proinflammatory. Both ethanol and isopropanol dampened inflammation except that 1% isopropanol treatment increased the IL-1β level by 26%. When reducing the BSA content in PA-BSA solutions from 3:1 to 5:1, a marked increase in cell viability (11%) was seen. To our surprise, reducing BSA content in PA-BSA solutions from 5:1 to 10:1 decreased cell viability by 11%. The 5:1 group exhibited the lowest inflammatory profile. Either PA-BSA or BSA alone increased the entry of LPS to the cytosol, which further caused pyroptosis. In summary, we found 5:1 (PA:BSA) to be the best binding ratio for studying inflammation in BV-2 microglia. The presence of LPS in the cytosol in the context of BSA might be the reason for confounding results from palmitate studies.
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JOURNAL OF NEUROSCIENCE
·
2023
·Vol. 43
·期 16
·2822-2836
·
IF 5.3
Jiao Ma,Zhechun Hu,Huimin Yue,Yujian Luo,Chao Wang,Xuan Wu,Yan Gu,Lang Wang
Metabotropic glutamate receptor 2 (GRM2) is highly expressed in hippocampal dentate granule cells (DGCs), regulating synaptic transmission and hippocampal functions. Newborn DGCs are continuously generated throughout life and express GRM2 when they are mature. However, it remained unclear whether and how GRM2 regulates the development and integration of these newborn neurons. We discovered that the expression of GRM2 in adult-born DGCs increased with neuronal development in mice of both sexes. Lack of GRM2 caused developmental defects of DGCs and impaired hippocampus-dependent cognitive functions. Intriguingly, our data showed that knockdown of Grm2 resulted in decreased b/c-Raf kinases and paradoxically led to an excessive activation of MEK/ERK1/2 pathway. Inhibition of MEK ameliorated the developmental defects caused by Grm2 knockdown. Together, our results indicate that GRM2 is necessary for the development and functional integration of newborn DGCs in the adult hippocampus through regulating the phosphorylation and activation state of MEK/ERK1/2 pathway.SIGNIFICANCE STATEMENT Metabotropic glutamate receptor 2 (GRM2) is highly expressed in mature dentate granule cells (DGCs) in the hippocampus. It remains unclear whether GRM2 is required for the development and integration of adult-born DGCs. We provided in vivo and in vitro evidence to show that GRM2 regulates the development of adult-born DGCs and their integration into existing hippocampal circuits. Lack of GRM2 in a cohort of newborn DGCs impaired object-to-location memory in mice. Moreover, we revealed that GRM2 knockdown paradoxically upregulated MEK/ERK1/2 pathway by suppressing b/c-Raf in developing neurons, which is likely a common mechanism underlying the regulation of the development of neurons expressing GRM2. Thus, Raf/MEK/ERK1/2 pathway could be a potential target for brain diseases related to GRM2 abnormality.
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CHEMICAL ENGINEERING SCIENCE
·
2021
·
IF 4.311
Gaobo Lin,Wenwen Lin,Jianghua Wu,You Zhan,Francis Okejiri,Mingwei Weng,Jie Fu
Biomass-derived 2,5-furandicarboxylic acid (FDCA) is considered a viable alternative to petroleum-derived terephthalic acid as a monomer in the production of bioplastic. FDCA is currently mainly produced via a two-step process involving the conversion of fructose to 5-hydroxymethylfurfural (HMF) and the subsequent oxidation of HMF to FDCA. However, the storage stability HMF is far below the industrial expectations, further inhibiting the HMF-mediated route. Herein, we report efficient oxidation of 5-methoxymethylfurfural (MMF) to FDCA over a homemade Ru/hydroxyapatite (Ru/HAP) catalyst. A complete conversion of MMF and > 65% molar yield of FDCA could be realized under mild reaction conditions of 130 °C and 1 MPa O 2 pressure. The effects of process parameters, including MMF/catalyst ratios, reaction temperature/time, and O 2 pressure, were systematically studied and optimized on MMF oxidation. A plausible reaction pathway was also proposed. This study and our previously reported fructose-to-MMF work may provide a feasible MMF-intermediated route for large-scale industrial FDCA production.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Xiang Xiao,Yurong Zhou,Cui Tan,Juan Bai,Ying Zhu,Jiayan Zhang,Xinghua Zhou,Yansheng Zhao
β-glucan is an important functional active component with relatively high content in barley. It is reported to possess various biological activities, including anti-oxidative stress, but its mechanism of action remains obscure. In the current study, C. elegans was used as an in vivo animal model to explore its anti-oxidative stress mechanism. We found that both RBG (raw barley β-glucan) and FBG (fermented barley β-glucan) could significantly reduce the ROS level in C. elegans under oxidative emergency conditions. In addition, both FBG and RBG had positive effects on SOD and CAT enzyme activity, and FBG treatment obviously reduced the MDA content in nematodes under oxidative stress. Moreover, FBG and RBG pretreatment could extend the median lifespan of C. elegans under oxidative stress. The CB1370 and CF1038 mutants further confirmed that daf-2 and daf-16 were necessary for FBG or RBG to participate in anti-oxidative stress, and the RT-PCR results also evidenced that β-glucans resist oxidative stress in C. elegans partially through the daf-2 / daf-16 pathway. In summary, barley β-glucan has high potential to defense oxidative stress as a natural polysaccharide.
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Analytical Methods
·
2023
·Vol. 16
·期 4
·576-582
·
IF 3.1
Fangfang Yang,Shuang Li,Jialiang Wu,Shufeng Liu
Reversible structural changes in DNA nanomachines have great potential in the field of bioanalysis. Here, we demonstrate an assembly strategy for quencher-free and tunable DNA tweezers based on 2-aminopurine (2-AP), avoiding the tedious fluorescence labelling step. The conformational state of the tweezers could be controlled by specific oligonucleotides (fuel or anti-fuel). Taking advantage of the local environmental sensitivity of 2-AP, the structural changes of the tweezers were easily tracked, and multiple cyclic switching of the tweezers between the open and closed states was achieved. In addition, the influence of oligonucleotide structure on the fluorescence properties of 2-AP was deeply explored. We figured out that the fluorescence of 2-AP was highly quenched by the base-stacking of natural bases in DNA oligonucleotides. Moreover, by comprehensively regulating the type of bases surrounding the inserted 2-AP site, a sensitive fluorescence response towards dynamic change can be obtained. This principle of quencher-free nanodevices based on 2-AP provides a convenient method for monitoring the structural changes of DNA nanomachines.
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