JOURNAL OF MICROENCAPSULATION
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2019
·
IF 2.04
Yingting Wang,Along Zuo,Xiaoyan Huang,Ying Ying,Xinsheng Shu,Xianxiong Chen,Yatao Yang,Junxian Ma,Guimiao Lin,Xiaomei Wang,Lin Mei,Gan Liu,Yingying Zhao
Taxane-based chemotherapy-loaded drug delivery systems have great potential for cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical cancer cell line Hela and the human breast cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and ‘self-controlled’ drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast cancer cells and human cervical cancer cells.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
Qimin Zhao,Jiayi Huang,Yongkang Yan,Danhua Ge,Tao Liu,Xiaojun Chen
Developing light-responsive nanozymes with integrated activity and stability holds significant importance for highly sensitive and accurate sensing in food safety and environmental protection. Herein, we developed a dual-mode (colorimetric and fluorescent) sensor based on an Fe-doped TiO2 nanosphere for d-penicillamine (D-PA) detection. The unique hollow structure of Fe-TiO2 provides a large surface area and abundant oxygen vacancies while facilitating rapid Fe(II)/Fe(III) cycling, collectively resulting in enhanced light-responsive peroxidase (POD)-like activity. Under light irradiation, the Fe-TiO2 promoted the generation of abundant reactive oxygen species, which oxidize TMB to blue oxTMB. Furthermore, the Fukui index and reaction kinetic trials indicated that the detection mechanism was primarily based on the antioxidant effect and redox activity of D-PA. The proposed sensor demonstrated detection limits (LODs) of 0.054 μM (colorimetric) and 0.40 μM (fluorescence), with 92.2–107.4% recovery and relative standard deviation < 4.4% in milk, tap water, and fetal bovine serum, along with outstanding reproducibility and stability. This work provides new insights to synthesize light-responsive nanozymes and integrated multimode sensing platforms.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Xipei Wu,Xiaojun Qiu,Shirui Wang,Nihui Zhang,Lingzhuo An,Peijie Song,Xia Li,Wenyuan Gao
Ethnopharmacological relevance Originally formulated to mitigate high-altitude sickness, Xinnaoxin capsules (XNX) are composed of three traditional Chinese medicines ( Rhodiola rosea L., Lycium barbarum L. and Hippophae rhamnoides ) with properties of anti-hypoxia, anti-fatigue, and anti-aging. Emerging evidence now suggests that XNX may also offer therapeutic benefits in Alzheimer’s disease (AD), highlighting its potential significance in the development of novel AD treatments. Aim of the study This study aims to investigate whether XNX improves AD-related behavioral and cognitive deficits by enhancing antioxidant defenses, reducing peripheral and neuroinflammation, and protecting neurons. Materials and methods The AD mouse model was established using D-galactose and aluminum chloride. Spatial memory and anxiety-like behaviors were assessed via the Morris water maze and open field tests to evaluate the therapeutic effects of XNX. Biochemical markers in hippocampal tissue and serum were measured using ELISA kits, while serum chemical composition was analyzed by LC-MS. Histopathological changes and amyloid-β deposition in the hippocampus were examined through hematoxylin-eosin (HE) staining and immunofluorescence. Additionally, hippocampal expression of apoptotic proteins Bax and Caspase-3, anti-apoptotic protein Bcl-2, and synaptic proteins PSD-95 and Syn were assessed via Western blot. Results Behavioral tests demonstrated that XNX significantly improved spatial learning and memory abilities, as well as reduced anxiety-like behaviors in AD mice. XNX also modulated inflammatory cytokines and oxidative stress markers in hippocampal tissue and serum, while reducing amyloid-β deposition. Further LC-MS analysis of serum revealed a marked upregulation of compounds such as adenosine following treatment, with key metabolic pathways affected, including linoleic acid metabolism and phenylalanine, tyrosine, and tryptophan biosynthesis. HE staining and immunofluorescence indicated that XNX ameliorated neuronal damage and decreased amyloid-β accumulation. Western blot analysis confirmed that XNX inhibited neuronal apoptosis and preserved synaptic proteins in the hippocampus. Conclusion XNX mitigates AD-induced behavioral and cognitive deficits by enhancing antioxidant defenses, reducing peripheral and neuroinflammation, and protecting neurons. Our findings provide valuable data and a theoretical foundation for the potential therapeutic application of XNX in AD treatment and its further development.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Zeng Yu,Jianbota Hanahati,Li Xuepeng,Zhang Hong,Lv Xuepeng
Alkali induction promotes the formation of fibrous gel network structure. • Increasing pH weakens the hydrogen bond and hydrophobic interactions of the gel. • To reveal the link between gel structure formed by different pH and VD 3 encapsulation stability. • Composite gel structural compactness inhibits VD 3 release during digestion. This study explored the effects of ultrasound-assisted alkaline treatment between the structure and vitamin D₃ (VD₃) encapsulation efficiency, together with in vitro release behavior of heat-induced egg yolk granule/whey protein composite gels. Results showed that the alkaline environment remarkably modulated the intermolecular conformation and aggregation behavior of composite proteins. With increasing pH, protein unfolding extent augmented, while surface hydrophobicity first decreased and then increased, peaking at pH 11. Enhanced electrostatic repulsion improved system dispersibility, accompanied by reduced particle size and elevated absolute zeta potential. Gel microstructure evolved from a spongy homogeneous porous matrix at pH 7 to a fibrous network at pH 11. Moreover, higher pH resulted in finer fibers, increased pore density, and enlarged average pore size. These changes were accompanied by the strengthening of disulfide bonds and hydrophobic interactions, which contributed to the formation of a robust elastic-dominated gel network. The highest VD 3 encapsulation efficiency (95.74%) was achieved at pH 9, where gels also exhibited the optimal VD₃ retention rate (26.18%) after 28 days of storage at 4 °C. In vitro digestion experiments indicated that pH 11 gels displayed the fastest release kinetics during gastric and intestinal phases, with a final cumulative release rate of 89.82%. In contrast, pH 7 and 9 gels showed sustained-release features, and their release behaviors were well fitted to kinetic models, respectively. In conclusion, ultrasound-assisted alkaline treatment optimizes composite gel network structure and functional attributes via precise pH modulation, providing a theoretical foundation for stable storage and controlled release of VD₃ in food matrixes.
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INORGANIC CHEMISTRY
·
2026
·
IF 4.6
Lin Wei,Yang Zhibin,Tao Jin-Gang-Lu,Zhao Qianqian,Liu Fuyu,Li Wanshan,Feng Renfei,Zhang Yu,Fu Xian-Zhu,Luo Jing-Li,Zhao Bin
Developing efficient and durable electrocatalysts for
alkaline
seawater hydrogen evolution remains challenging because of sluggish
water dissociation kinetics. Herein, we report a defect-rich d-p orbital-hybridized
Os/o-CNTs electrocatalyst ( dph -Os def /o-CNTs)
prepared by rapid room-temperature NaBH 4 reduction. The
partially oxidized CNT support anchors defective Os nanoclusters and
induces C–Os/C–O–Os electronic coupling, generating
electron-deficient Os sites with modulated 5d electronic structures.
In alkaline simulated seawater (1 M NaOH + 3.5 wt % NaCl), dph -Os def /o-CNTs requires only 91 mV to reach
100 mA cm –2 and exhibits a Tafel slope of 19 mV
dec –1 , with stable operation over 350 h and 10,000
CV cycles. In situ Raman spectroscopy, AIMD simulations, and DFT calculations
reveal that Os vacancies reorganize interfacial water into an ordered
H-bond network, promote O-down water adsorption, and reduce the water
dissociation barrier from 1.12 to 0.56 eV. Moreover, an Al | OH – | H 2 primary cell using dph -Os def /o-CNTs as the cathode delivers 1.41 V and 10.92
mW cm –2 , enabling simultaneous hydrogen generation
and electricity output.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Fengge Yang,Danmei Tian,Yuting Zhang,Zixuan Xia,Yunjuan Li,Xinsheng Yao,Yanxia Xiong,Guirong Zhou,Hongwei Liu,Jinshan Tang
CKF ameliorates intestinal dysfunction and neurobehavioral abnormalities in IBS-D mice. • CKF boosts L. johnsonii and tryptophan-derived indoles, notably ILA. • L. johnsonii acts as a psychobiotic for IBS-D and comorbidities. • ILA improves IBS-D intestinal and behavioral deficits mainly via AhR activation. • CKF targets the L. johnsonii -ILA-AhR/IL-22 gut-brain axis. Background Gut dysbiosis contributes to irritable bowel syndrome (IBS) by disrupting intestinal barrier functions and promoting inflammation. Chang‑kang‑fang (CKF), a Chinese herbal formula proven effective for diarrhea‑predominant IBS (IBS‑D) in a phase III trial, is thought to modulate the gut microbiota, yet the underlying mechanism remains unclear. Purpose To elucidate how CKF ameliorates IBS‑D by modulating the gut microbiota. Methods IBS‑D was induced by restraint stress, tail pinch, and senna gavage. Intestinal function, inflammation, and behavior were evaluated. Gut microbiota and metabolites were profiled via 16S rRNA sequencing and metabolomics. FMT confirmed the causal involvement of the microbiota. The key microbe and metabolite were validated in vivo . Mechanistic analyses evaluated intestinal barrier integrity, systemic inflammation, hippocampal blood‑brain barrier (BBB) function, neuroinflammation, and synaptic plasticity. Results CKF alleviated diarrhea, visceral hypersensitivity, inflammation, barrier dysfunction, and anxiety‑ and depression‑like behaviors. These benefits were associated with gut microbiota remodeling, featuring enrichment of Lactobacillus johnsonii and enhanced tryptophan metabolism. FMT confirmed that this microbial remodeling mediates the therapeutic effects of CKF. Supplementation with L. johnsonii or its metabolite indole‑3‑lactic acid (ILA) largely recapitulated these gut-brain benefits via AhR/IL‑22 activation. Mechanistically, CKF‑induced L. johnsonii enrichment activated the ILA-AhR-IL‑22 pathway, which enhanced intestinal and BBB integrity, thereby attenuating systemic and neuroinflammation, and restoring hippocampal synaptic plasticity. Conclusion This study positions CKF as a microbiota‑modulating therapy for IBS‑D that restores gut‑brain homeostasis via the L. johnsonii -ILA-AhR-IL‑22 axis, highlighting L. johnsonii and ILA as potential therapeutics for IBS and its comorbidities. Download: Download high-res image (278KB) Download: Download full-size image Chang-Kang-Fang alleviates IBS-D and comorbid depression by activating the Lactobacillus johnsonii -indole-3-lactic acid-aryl hydrocarbon receptor/interleukin-22 gut-brain axis.
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International Journal of Nanomedicine
·
2023
·
IF 8
Jing Lin,Chaoyue Zhao,Cuijuan Liu,Shiyao Fu,Luying Han,Xinping Lu,Chunrong Yang
Introduction The development of nanodrug carriers utilizing tumor microenvironment has become a hotspot in reversing multidrug resistance (MDR).Materials and methods This study synthesized a redox-sensitive copolymer, Pluronic F127-disulfide bond-d-α-tocopheryl polyethylene glycol 1000 succinate (FSST), through the connection of the reduction-sensitive disulfide bond between F127 and d-α-tocopheryl polyethylene glycol 1000 succinate. This polymer could induce the elevation of reactive oxygen species (ROS) levels, ultimately resulting in cytotoxicity. Moreover, the redox-responsive mixed micelles, F127-folate (FA)/FSST/P123 (FFSSTP), based on FSST, Pluronic F127-FA, and Pluronic P123, were prepared to load paclitaxel (PTX).Results The in vitro release study demonstrated that FFSSTP/PTX accelerated the PTX release through the breakage of disulfide bond in reductive environment. In cellular experiment, FFSSTP/PTX induced significant apoptosis in PTX-resistant MCF-7/PTX cells through inhibiting adenosine triphosphate (ATP)-binding cassette proteins from pumping out PTX by interfering with the mitochondrial function and ATP synthesis. Furthermore, FFSSTP/PTX induced apoptosis through elevating the intracellular levels of ROS.Conclusion FFSSTP could become a potential carrier for the treatment of MDR tumors.
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高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 11
·2307-2320
·
IF 5.344
Xiaonan Li,Ziming Zhao,Yihua Yang,Zhaorong Liu,Jinglei Wang,Yalu Xu,Yanzhuo Zhang
In this study, a new type of β-1,3-D-glucan porous microcapsule (GPM)-enveloped and folate conjugated chitosan-functional liposome (FCL), FCL@GPM, was developed for the potential oral co-delivery of chemotherapeutic drugs and quantum dots (QDs) with facilitated drug absorption and antitumor efficacy. In this dual-particulate system, multiple FCLs serve as the cores for effective loading, folate-mediated tumor-targeting, facilitated intracellular accumulation, and pH-responsive controlled release of chemotherapeutic agents, while a GPM acts as the shell for affording macrophage-mediated tumor selectivity. Gefitinib (GEF) was selected as a chemotherapeutic agent, while acid degradable ZnO QDs were selected due to their dual role as an anticancer agent for synergistic chemotherapy and as a fluorescent probe for potential cancer cellular imaging. The GEF and ZnO QD co-loaded FCL@GPMs (GEF/ZnO-FCL@GPMs) exhibited a prolonged release manner with limited release before uptake by intestinal cells. Furthermore, Peyer's patch uptake, macrophage uptake, cytotoxicity, and biodistribution of FCL@GPMs were tested. In addition, GEF and ZnO QD co-loaded FCLs (GEF/ZnO-FCLs) not only have a tumor acidity responsive release property, but also induce a superior cytotoxicity on cancer cells as compared to GEF. Moreover, a 1.75-fold increase in the bioavailability of GEF delivered from GEF/ZnO-FCL@GPMs as compared to its trademarked drug (Iressa®). As a result, GEF/ZnO-FCL@GPMs exerted a superior antitumor efficacy (1.47-fold) as compared to the trademarked drug in mice. Considered together, the developed FCL@GPMs, combining the unique physicochemical and biological benefits of FCLs and GPMs, possess great potential as an efficient delivery system for the co-delivery of chemotherapeutic agents and quantum dots.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Chuan Zhao,Chaozhu Shu,Ruixing Zheng,Dayue Du,Longfei Ren,Miao He,Runjing Li,Haoyang Xu,Xiaojuan Wen,Jianping Long
Developing effective electrocatalyst and fundamentally understanding the corresponding working mechanism are both urgently desired to overcome the current challenges facing lithium-oxygen batteries (LOBs). Herein, a series of NiFe-based bimetal-organic frameworks (NiFe-MOFs) with certain internal tensile strain are fabricated via a simple organic linker scission strategy, and served as cathode catalysts for LOBs. The introduced tensile strain broadens the inherent interatomic distances, leading to an upshifted d-band center of metallic sites and thus the enhancement of the adsorption strength of catalysts surface towards intermediates, which is contributed to rationally regulate the crystallinity of discharge product Li 2 O 2 . As a result, the uniformly distributed amorphous film-like Li 2 O 2 tightly deposits on the surface of strain-regulated MOF, resulting in excellent electrochemical performance of LOBs, including a large discharge capacity of 12317.4 mAh g −1 at 100 mA g −1 and extended long-term cyclability of 357 cycles. This work presents a novel insight in adjusting the adsorption strength of cathode catalysts towards intermediates via introducing tensile strain in catalysts, which is a pragmatic strategy for improving the performance of LOBs.
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NUCLEAR MEDICINE AND BIOLOGY
·
2018
·
IF 2.203
Weiyan Zhou,Weiqi Bao,Donglang Jiang,Yanyan Kong,Fengchun Hua,Xiuhong Lu,Yihui Guan
Introduction The objective of this study was to synthesize an N -methyl- d -aspartate receptor (NMDAR) radiotracer [ 18 F]-GE-179 in one-pot and evaluate its in vivo binding for NMDAR activation after brain ischemia reperfusion injury. Methods [ 18 F]-GE-179 was auto-synthesized using a quick one-pot method from a stable disulfide precursor and purified using semi-preparative high-performance liquid chromatography (HPLC) with ethanol/aqueous NaH 2 PO 4 as the eluent. Dynamic micro-positron emission tomography (PET)/computed tomography (CT) study of [ 18 F]-GE-179 was successfully performed using a rat model of middle cerebral artery occlusion (MCAO, induced by transient occlusion into the left MCA). A simplified reference tissue model method was used to calculate the [ 18 F]-GE-179 non-displaceable binding potential (BPND) for intracranial NMDAR activation assessment. Immunofluorescence staining of NMDAR NR1 subunit in brain slices containing lesion was also performed. Results [ 18 F]-GE-179 was successfully prepared in a yield of 23–30% in a formulation that could be injected directly after dilution. Localized radioactivity accumulation was observed in the animal model of MCAO. Significantly higher ( p = 0.0003–0.0404) BPND relative to equivalent contralateral was found in the ipsilateral caudate putamen , Acb core/shell, cortex cingulate , amygdala , hypothalamus , and superior colliculus . Immunofluorescence staining showed elevated NMDAR expression in the affected hemisphere. Conclusions [ 18 F]-GE-179 was synthesized using a one-pot process with a markedly improved yield. Preliminary in vivo micro-PET study visualized excessive NMDAR stimulation successfully in a rodent model of MCAO, which was consistent with results of in vitro immunofluorescence staining. The study demonstrates that [ 18 F]-GE-179 is a promising PET probe for the detection of functional NMDAR alterations.
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INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Shudong He,Zuoyong Zhang,Hanju Sun,Yuchen Zhu,Jinlong Zhao,Mingming Tang,Xiangyu Wu,Yanping Cao
Rapeseed peptide was used to produce flavor enhancer via heating with d -xylose at various temperatures (80–140 °C) at reaction pH 7.4 for 2 h with or without l -cysteine (RXC or RX). Effects of temperatures and l -cysteine on the Maillard reaction products (MRPs) were investigated by the determinations of pH, color, free amino acids (FAA), and volatile compounds, as well as E-nose and E-tongue analyses. Partial least square regression (PLSR) method was further applied to explore the correlations. Results suggested that pH decreased more significantly in RXCs, and a more dark brown color was observed in RXs with the increase of heating temperatures. The lowest bitterness MRPs were obtained by heating at 140 °C with cysteine (RXC-140), while the highest umami and saltiness sample was found in RX-100. Bitter and umami FAAs were present in greater amounts at higher temperatures with cysteine. Furthermore, RXC showed a meat-like flavor due to the generation of sulfur-containing compounds, and more furans and nitrogen-containing compounds were detected in RX, along with a caramel-like flavor.
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ACS Nano
·
2024
·
IF 15.8
Xuelei Wang,Xiaowei Wang,Jiayi Su,Dan Wang,Wenkai Feng,Xiaobo Wang,Hongwei Lu,Apeng Wang,Mingliang Liu,Guimin Xia
Acute myeloid leukemia (AML) is a hematological malignancy with a high recurrence rate. The interaction of chemokine receptor 4/chemokine ligand 12 (CXCR4/CXCL12) mediates homing and adhesion of AML cells in bone marrow, leading to minimal residual disease in patients, which brings a hidden danger for future AML recurrence. Ara-C is a nonselective chemotherapeutic agent against AML. Due to its short half-life and severe side effects, a lipid-like Ara-C derivative (AraN) was synthesized and a dual-function LipoAraN-E5 (135 nm, encapsulation efficiency 99%) was developed, which coloaded AraN and E5, a peptide of the CXCR4 antagonist. LipoAraN-E5 effectively improved the uptake, enhanced the inhibition of leukemia cell proliferation, migration, and adhesion to stromal cells in bone marrow, and mobilized the leukemia cells from bone marrow to peripheral blood via interfering with the CXCR4/CXCL12 axis. LipoAraN-E5 prolonged the plasma half-life of AraN (8.31 vs 0.56 h) and was highly enriched in peripheral blood (3.67 vs 0.05 μmol/g at 8 h) and bone marrow (379 vs 148 μmol/g at 24 h). LipoAraN-E5 effectively prevented the infiltration of leukemia cells in peripheral blood, bone marrow, spleen, and liver, prolonged the mice survival, and showed outstanding antineoplastic efficacy with negligible toxicity, which were attributed to the ingenious design of AraN, the use of a liposomal delivery carrier, and the introduction of E5. Our work revealed that LipoAraN-E5 may be a promising nanocandidate against AML.
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危险品化学品经营许可证（带存储）