Horticultural Plant Journal
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2020
·
IF 1.524
Mingyue Zhao,Binbin Cai,Jieyang Jin,Na Zhang,Tingting Jing,Jingming Wang,Yuting Pan,Zixiang Zhou,Yifan Zhao,Yingying Feng,Feng Yu,Mengting Zhang,Yating Li,Zhonghua Liu,Chuankui Song
Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants. Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality. However, the details of their biosynthesis and metabolism in tea plants are still unknown. Here, eugenol glucoside was unambiguously identified as a native metabolite in the tea plant, and its biosynthesis was shown to be induced by low temperature treatment. Through the analysis of UGTs induced by low temperature, the glycosyltransferase CsUGT78A15 was identified in tea, and its encoded protein was shown to catalyze the glucosylation of eugenol. V max / K m ratios showed that eugenol was the most suitable substrate for CsUGT78A15. Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose, followed by galactose and glucuronic acid. The expression of CsUGT78A15 was correlated with the accumulation of eugenol glucoside in different tissues and genotypes of tea. Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress, indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress. The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.
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ACTA PHARMACOLOGICA SINICA
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2020
·Vol. 42
·期 6
·987-997
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IF 5.064
Liu Qiu-yan,Zhuang Yu,Song Xian-rui,Niu Qun,Sun Qiu-shuang,Li Xiao-nan,Li Ning,Liu Bao-lin,Huang Fang,Qiu Zhi-xia
Metabolic reprogramming is associated with NLRP3 inflammasome activation in activated macrophages, contributing to inflammatory responses. Tanshinone IIA (Tan-IIA) is a major constituent from Salvia miltiorrhiza Bunge, which exhibits anti-inflammatory activity. In this study, we investigated the effects of Tan-IIA on inflammation in macrophages in focus on its regulation of metabolism and redox state. In lipopolysaccharides (LPS)-stimulated mouse bone marrow-derived macrophages (BMDMs), Tan-IIA (10 μM) significantly decreased succinate-boosted IL-1β and IL-6 production, accompanied by upregulation of IL-1RA and IL-10 release via inhibiting succinate dehydrogenase (SDH). Tan-IIA concentration dependently inhibited SDH activity with an estimated IC 50 of 4.47 μM in LPS-activated BMDMs. Tan-IIA decreased succinate accumulation, suppressed mitochondrial reactive oxygen species production, thus preventing hypoxia-inducible factor-1α (HIF-1α) induction. Consequently, Tan-IIA reduced glycolysis and protected the activity of Sirtuin2 (Sirt2), an NAD + -dependent protein deacetylase, by raising the ratio of NAD + /NADH in activated macrophages. The acetylation of α-tubulin was required for the assembly of NLRP3 inflammasome; Tan-IIA increased the binding of Sirt2 to α-tubulin, and thus reduced the acetylation of α-tubulin, thus impairing this process. Sirt2 knockdown or application of Sirt2 inhibitor AGK-2 (10 μM) neutralized the effects of Tan-IIA, suggesting that Tan-IIA inactivated NLRP3 inflammasome in a manner dependent on Sirt2 regulation. The anti-inflammatory effects of Tan-IIA were observed in mice subjected to LPS challenge: pre-administration of Tan-IIA (20 mg/kg, ip) significantly attenuated LPS-induced acute inflammatory responses, characterized by elevated IL-1β but reduced IL-10 levels in serum. The peritoneal macrophages isolated from the mice displayed similar metabolic regulation. In conclusion, Tan-IIA reduces HIF-1α induction via SDH inactivation, and preserves Sirt2 activity via downregulation of glycolysis, contributing to suppression of NLRP3 inflammasome activation. This study provides a new insight into the anti-inflammatory action of Tan-IIA from the respect of metabolic and redox regulation.
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Frontiers in Cellular Neuroscience
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2020
·
IF 3.921
Xu Yi,Zhang Shuai,Sun Qiang,Wang Xu-Qiao,Chai Ya-Ni,Mishra Chandan,Chandra Shah Ram,Ai Jing
Chronic cerebral hypoperfusion (CCH) is considered a preclinical condition of mild cognitive impairment and thought to precede dementia. However, as the principal cholinergic source of hippocampus, whether the septo-hippocampal neurocircuit was impaired after CCH is still unknown. In this study, we established the CCH rat model by bilateral common carotid artery occlusion (2VO). Under anesthesia, the medial septum (MS) of rats was stimulated to evoke the field excitatory post-synaptic potential (fEPSP) in the pyramidal cell layer of dCA1. Consequently, we observed decreased amplitude of fEPSP and increased paired-pulse ratio (PPR) after 8-week CCH. After tail pinch, we also found decreased peak frequency and shortened duration of hippocampal theta rhythm in 2VO rats, indicating the dysfunction of septo-hippocampal neurocircuit. Besides, by intracerebroventricularly injecting GABAergic inhibitor (bicuculline) and cholinergic inhibitors (scopolamine and mecamylamine), we found that CCH impaired both the pre-synaptic cholinergic release and the post-synaptic nAChR function in MS–dCA1 circuits. These results gave an insight into the role of CCH in the impairment of cholinergic MS–dCA1 neurocircuits. These findings may provide a new idea about the CCH-induced neurodegenerative changes.
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Journal of Cleaner Production
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2021
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IF 9.297
Yafei Zhang,Hui Liu,Keyong Tang,Jie Liu,Xiumin Li
Large amounts of water and leather chemicals such as sodium sulfide and calcium hydroxide are used in conventional leather production, resulting in huge amount of wastewater and serious environmental pollution . Enzyme is eco-benign, and an ideal alternative to conventional chemicals attributed to its excellent characteristics and high efficiency. In this work, enzymes with different ions are used in beamhouse to remove the interfibrillar substance and open collagen fiber bundles. Analysis of the opened collagen fibers shows that the enzyme with both hydrogen phosphate and magnesium ions have the best effect in opening collagen fiber bundles. Results of wastewater analysis and the staining of hematoxylin-eosin (H&E) and Verhoeff’s Van Gieson (EVG) indicated the excellent removal of interfibrillar substance by all ion-assisted trials, suggesting an improved removal efficiency. The mechanical properties and shrinkage temperature of the leathers are similar to those of the conventional leathers. Since both deliming and bating are no longer required, a large amount of water can be saved, the leather making time can be shortened, and the cost of wastewater treatment can be reduced. Ion-assisted enzyme method is more efficient and environmentally friendly than the conventional one with sodium sulfide. This work presents a promising path for the cleaner leather making.
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RSC Advances
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2021
·Vol. 11
·期 6
·3770-3776
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IF 3.361
Guangtao Qian,Fengna Dai,Haiquan Chen,Mengxia Wang,Mengjie Hu,Chunhai Chen,Youhai Yu
5-Amine-2-(4-amino-benzene)-1-phenyl-benzimidazole (N-PhPABZ) was successfully synthesized and polymerized with 3,3′,4,4′-biphenyl tetracarboxylic dianhydride (BPDA) to obtain a novel N-phenyl-poly(benzimidazole imide) (N-Ph-PBII). The successful incorporation of N-phenyl addressed the issue of high H2O-absorption of traditional PBIIs while retained the superheat resistance property. The resulting N-Ph-PBII possessed a high glass-transition temperature (Tg) up to 425 °C and a low affinity for water of 1.4%. Furthermore, the loose molecular packing and noncoplanar structures led to an increase in optical transparency for the modified PBII.
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FOOD CHEMISTRY
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2020
·
IF 6.306
Pengfei Wang,Naiyi Yin,Xiaolin Cai,Huili Du,Yaqi Fu,Ziqi Geng,Sharmin Sultana,Guoxin Sun,Yanshan Cui
Rice, a staple food for half the world’s population, easily accumulates arsenic (As). Research on As distribution in rice protein and starch and its relationship with rice As bioaccessibility remains limited. This study investigated As distribution, chemical composition, As bioaccessibility and speciation in rice by continuous extraction and in vitro digestion. Of the total As, 87.5–94.5% was in rice protein and 5.0–9.8% in rice starch. The As amount in different protein fractions decreased as follows: glutelin > globulin > albumin > prolamin. As(V), As(III) and DMA in rice were more bioaccessible in the small intestinal phase than the gastric phase, and almost all As(V) dissolved in the small intestinal phase. Bioaccessible As in gastrointestinal digestive solution and As mass in protein fractions (albumin, globulin, and glutelin) were significantly positively correlated ( p < 0.05). These results illuminate the bioaccessibility of As to humans consuming As-contaminated rice and avoid overassessment.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Yifan Li,Hong Sun,Zhen Tian,Yue Li,Xuan Ye,Ruichi Li,Xinyu Li,Shengli Zheng,Jiyuan Liu,Yalin Zhang
The long-term and excessive use of insecticides has led to severe environmental problems and the evolution of insecticide resistance in insects. Carboxylesterases (CarEs) are important detoxification enzymes conferring insecticide resistance on insects. Herein, the detoxification process of Plutella xylostella (L.) carboxylesterase 6 (PxEst-6), one representative P. xylostella carboxylesterase, is investigated with cypermethrin, bifenthrin , cyfluthrin and λ-cyhalothrin. RT-qPCR shows that PxEst-6 is highly expressed in the midgut and cuticles of the third instar larvae. Exposure to pyrethroid insecticides resulted in PxEst-6 up-regulation in a short time. Metabolic assays indicate that PxEst-6 has the capacity to metabolize these pyrethroid insecticides. The combination of molecular docking, binding mode analyses and alanine mutations demonstrated that His451, Lys458 and Gln431 were key residues of PxEst-6 for metabolizing pyrethroids and the acetate groups derived from pyrethroids were key sites for being metabolized by PxEst-6. H451- and K458-derived hydrogen bond (H-bond) interactions with the pyrethroid acetate groups and the polar interactions with the pyrethroid acetate group provided by the Q431 sidechain were crucial to the pyrethroids’ metabolism by PxEst-6. Our study contributes to revealing the reasons for pyrethroid resistance in P. xylostella , and provides a fundamental basis for the development of novel pyrethroid insecticides.
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FOOD CHEMISTRY
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2020
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IF 6.306
Ibrahim Khalifa,Wei Zhu,Asad Nawaz,Kaikai Li,Chunmei Li
The effects of mulberry anthocyanins (MAs) on the digestibility of whey proteins (WP) in freshly-prepared and stored energy balls were studied. Results showed that MAs increased digestibility of the energy balls by increasing their hydrolysis-degree, soluble peptides-fractions, and decreasing their particle's size and agglomeration. To understand the mechanism of the promoting and/or inhibiting digestive effects of MAs, secondary structure alterations and binding of WP-MAs-mixtures were therefore measured. Results revealed that MAs could noncovalently/covalently interact with WP and form WP-MAs-adducts. This interaction seemed to be responsible for the alterations in the secondary structure of WP which could promote the digestibility of the energy balls subsequently. MAs also partially unfolded the structure of digested-WP through fluctuating their α-helix and β-sheet. It was concluded that the unfolding in WP-structure induced by MAs-interactions might increase accessibility of the peptide bonds to the digestive enzymes and consequentially facilitate the protein’s digestibility in the energy balls.
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Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 10
·6432-6441
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IF 12.732
Weiji Dai,Xiaowan Bai,Yin-an Zhu,Yue Zhang,Tao Lu,Ye Pan,Jinlan Wang
Surface reconstruction of non-oxide electrocatalysts for the oxygen evolution reaction (OER) to form “true” active species has been reported; however, the mechanism of the in situ surface activation has remained unclear. In this work, nanocrystalline Ni5P4 is prepared as a pre-catalyst for the OER to gain insight into the in situ surface activation. We find that NiO nanosheets with abundant crystal defects are formed on the surfaces of Ni5P4 particles during the electrochemical process. The effects of the in situ P incorporation in surface reconstruction derived NiO for OER electrocatalysis are discussed. Theoretical calculations reveal that the heteroatom P substitution for O atoms of NiO crystals on the subsurface can weaken the binding strength of the OER intermediates, change the potential-determining step of the OER and achieve a lower theoretical overpotential. The present work provides a novel mechanism of the enhanced electrocatalytic performances of non-oxide materials for OER electrocatalysis by highlighting the effects of surface reconstruction induced in situ heteroatom doping in derived active materials.
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JOURNAL OF MEMBRANE SCIENCE
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2020
·
IF 7.183
Shuai Wang,Zhong-yang Wang,Jian-zhong Xia,Xiao-mao Wang
Membrane surface properties play important roles in determining the rate and severity of membrane fouling . This paper reported an innovative nanofiltration (NF) membrane called PENF which had a poly(piperazine-amide) active layer formed on a polyethylene (PE) support layer. The PENF membrane was very attractive in that millimeter-sized surface patterns could be automatically formed by the pressing effect of applied pressure for which permeate spacer acted as the in situ imprinter. This was attributed to the thin and tough PE support layer, which could be easily deformed under the applied pressure for NF applications. The surface-patterned PENF membrane (PENF-P) demonstrated greatly enhanced resistance to organic, inorganic and scaling fouling as well as combined fouling, when compared to either the original PENF membrane or commercial NF membranes like NF270. Without surface patterns, the PENF membrane along with other NF membranes followed a same general rule in resisting membrane fouling; a more hydrophilic NF membrane was generally more resistant to fouling. Membrane integrity breaches caused by surface deformation and patterning were negligible in terms of glucose rejection and water permeability coefficient. The PENF-P membrane could reject glucose by >80% at an applied pressure of 3.5 bar and had a water permeability coefficient of >11 L/m 2 /h/bar. A continuous running of a small-size spiral-wound element of the membrane (0.85 m 2 ) for over 3000 h confirmed that the PENF membrane had a long-time consistently high performance in terms of water permeation and solute rejection. The innovative PENF membrane and the involved surface patterning technique are expected to have a bright application prospect for water treatment and wastewater reclamation.
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CHEMOSPHERE
·
2020
·
IF 5.778
Mengjie Wang,Haojing Zhang,Weifeng Chen,Taotao Lu,Huihui Yang,Xinhai Wang,Minghua Lu,Zhichong Qi,Deliang Li
Since iron oxide minerals are ubiquitous in natural environments, the release of graphene oxide (GO) into environmental ecosystems can potentially interact with iron oxide particles and thus alter their surface properties, resulting in the change of their transport behaviors in subsurface systems. Column experiments were performed in this study to investigate the co-transport of GO nanoparticles and hematite colloids (a model representative of iron oxides) in saturated sand. The results demonstrated that the presence of hematite inhibited GO transport in quartz sand columns due to the formation of less negatively charged GO-hematite heteroaggregates and additional deposition sites provided by the adsorbed hematite on sand surfaces. Contrarily, GO co-present in suspensions significantly enhanced the transport of hematite colloids through different mechanisms such as the increase of electrostatic repulsion, decreased physical straining, GO-facilitated transport of hematite (i.e., highly mobile GO nanoparticles served as a mobile carrier for hematite). We also found that the co-transport behaviors of GO and hematite depended on solution chemistry (e.g., pH, ionic strength, and divalent cation (i.e., Ca 2+ )), which affected the electrostatic interaction as well as heteroaggregation behaviors between GO nanoparticles and hematite colloids. The findings provide an insight into the potential fate of carbon nanomaterials affected by mineral colloids existing in natural waters and soils.
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Molecular Catalysis
·
2020
·
IF 3.687
Ping Wei,Peng Chao,Yao-Ying Wang,Dong-Li Li,Qing-Jian Zou,Min-Hua Zong,Wen-Yong Lou
( S )-1-Phenyl-1,2-ethanediol (PED) is a vital chiral block in specialty chemical industries. The biotransformation of 2-hydroxyacetophenone (2-HAP) to ( S )-PED was conducted successfully catalyzed with BL21(DE3)(pETDuet- gst -mut- accr - gdh ) which harboring a carbonyl reductase mutant (mut-AcCR). The catalytic activity of the biocatalyst was 15.9 times higher than the recombinant cells harboring original AcCR. 250 mM 2-HAP was reduced, under optimal conditions, and the yield and space-time yield were 95.2 % and 1.89 M/d in the buffer system. Then C 4 MIMI·PF 6 was chosen to be the second phase to improve the catalytic efficiency, and the substrate concentration was increased to 450 mM in the C 4 MIMI·PF 6 /buffer system. After a reaction duration of 3 h, the product yield was over 92 %, and the space-time yield increased to 2.88 M/d which was 1.52 folds higher than that in the buffer system. Also, the product e.e. was over 99 % consistently. Overall, the preparative scale reduction of 2-HAP (450 mM) in C 4 MIMI·PF 6 /buffer system was conducted with promising result. The developed effective C 4 MIMI·PF 6 /buffer system of asymmetric reduction catalyzed by BL21(DE3)(pETDuet- gst -mut- accr - gdh ) will promote the preparation of the enantiomeric pureity chiral alcohol at industrial scale.
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