CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 39
·期 1
·122-132
·
IF 3.154
Yu Man-Man,Yang Wei-Jun,Niu De-Yu,Cai Xiao-Xia,Weng Yun-Xuan,Dong Wei-Fu,Chen Ming-Qing,Xu Peng-Wu,Wang Yang,Chu Hong,Ma Pi-Ming
In this work, hydroxyl-terminated oxalamide compounds N 1 , N 2 -bis(2-hydroxyethyl)oxalamide (OXA1) and N 1 , N 1′ -(ethane-1,2-diyl)bis( N 2 -(2-hydroxyethyl)oxalamide (OXA2) were synthesized to initiate the ring-opening polymerization of L-lactide for preparation of oxalamide-hybridized poly(L-lactide) (PLA OXA ), i.e. , PLA OXA1 and PLA OXA2 . The crystallization properties of PLA were improved by the self-assembly of the oxalamide segments in PLA OXA which served as the initial heterogeneous nuclei. The crystal growth kinetics was studied by Hoffman-Lauritzen theory and it revealed that the nucleation energy barrier of PLA OXA1 and PLA OXA2 was lower than that of PLA. Consequently, PLA OXA could crystallize much faster than PLA, accompanied with a decrease in spherulite size and half-life crystallization time by 74.8% and 86.5% (T = 125 °C), respectively. In addition, the final crystallinity of PLA OXA1 and PLA OXA2 was 6 and 8 times higher, respectively, in comparison with that of neat PLA under a controlled cooling rate of 10 °C/min. The results demonstrate that the hybridization of oxalamide segments in PLA backbone will serve as the self-heteronucleation for promoting the crystallization rate. The higher the content of oxalamide segments (PLA OXA2 compared with PLA OXA1 ) is, the stronger the promotion effect will be. Therefore, this study may provide a universal approach by hybridizing macromolecular structure to facilitate the crystallization of semi-crystalline polymer materials.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Shuo Shi,Wentao Zhang,Xinnan Liu,Xinyi Ren,Min Li,Jing Sun,Yutang Wang,Tianli Yue,Jianlong Wang
Lycium barbarum L. polysaccharides (LBPs) with outstanding biological activities are of increasing interest. Traditional purification approaches are time-consuming and often involve toxic solvents that destroy the functionality and structure of polysaccharides. Herein, we report a sustainable and nondestructive strategy for purifying LBPs using graphene-based nano-decoloration. The amination of graphene oxide (GO) enables the resulted aminated reduced GO (NH 2 -rGO) with abundant sp 2 -hybridized carbon domains, displaying high adsorption capacity toward pigments in crude polysaccharides. As such, within 5 min, NH 2 -rGO can highly effectively and fast to decolor LBPs, with a high decoloration ratio of 98.72% and a high polysaccharides retention ratio of 95.62%. Importantly, compared with traditional decoloration methods, NH 2 -rGO is nondestructive toward LBPs and has good reusability. Moreover, it exhibited widespread-use decoloration performance to decolor several common plant species. Overall, our proposed nano-decoloration approach is a general-purpose, sustainable, and nondestructive method to purify LBPs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2020
·
IF 3.693
Chen Pin,Kong Jingyang,Wang Xin,Ma Weiye,Yang Xia,Qin Yuqing,Hu Xiaohong
Synthesized polypeptide is attracting an increased interests due to its excellent biological characteristic and adjustable chemical properties in bio-related fields. But polypeptide itself has no switching properties, which is harmful to the development of its application as a control component. Herein, light-responsive poly(γ-benzyl-L-glutamate)s (PBLGs) is synthesized by a one-step NCA method using p-aminoazobenzene (m-AZO) and p-diaminoazobenzene (m-DAZO) as initiators. PBLGs exhibit amorphous characteristics with obvious Tg transition, which are 14°C for PBLG1 and 21°C for PBLG2. In order to forecast the structure-property information of PBLGs, theoretical UV-vis spectra as well as the energy gap between HOMO and LUMO is calculated by DFT calculation. Experimental results of UV-vis spectra exhibit similar characteristics to those of theorical UV-vis spectra except for the 40–50 nm red-shifting of absorbance peak. Furthermore, the absorbance intensities of PBLGs have a good linear relationship with their concentration, but their linearity range depending on concentration is completely different. Then, trans–cis transition under a different excitation source and cis–trans recovery in a dark environment are tracked in real-time by UV-vis spectra to evaluate the light response performances. It is found that UV light is the only effective excitation source for PBLG1, and blue light is another effective excitation source for PBLG2 besides UV light. Furthermore, the addition of alcohol and water as cosolvents has little effect on trans→cis transition in UV-light-excited systems, but it shortens recovery time of the cis→trans process in a dark environment. By contrast, the detectable isomerization process becomes unclear with the addition of alcohol in blue-light-excited system. Furthermore, either alcohol or water in solvents accelerate both the trans→cis and cis→trans process in a blue-light-excited system.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Siyi Li,Na Liang,Pengfei Yan,Yoshiaki Kawashima,Shaoping Sun
Insulin is the most effective drug in the treatment of diabetes mellitus. At present, subcutaneous injection is still the common way for insulin delivery. However, oral delivery is considered as the most preferred way for its high patient compliance and the minimal invasiveness. In this study, a novel N -acetyl- L -cysteine and arginine modified hydroxypropyl-β-cyclodextrin (NAC-HP-β-CD-Arg) was successfully synthesized and characterized. The polymer was used as a carrier for oral delivery of insulin by forming NAC-HP-β-CD-Arg@insulin complex. Enzymatic degradation study indicated that the NAC-HP-β-CD-Arg could protect insulin from enzymolysis. Moreover, the polymer exhibited strong binding ability with mucin. The transportation efficiency of NAC-HP-β-CD-Arg@insulin across the Caco-2 cell monolayer was much greater than free insulin. The in vivo study demonstrated that the orally administered NAC-HP-β-CD-Arg@insulin exhibited an excellent and sustained hypoglycemic effect in diabetic rats. It can be concluded that the NAC-HP-β-CD-Arg is a potential carrier for oral delivery of insulin.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2020
·
IF 2.428
Liping Yang,Shaoyang Zhi,Guokun Yang,Chaobin Qin,Wenli Zhao,Mingming Niu,Wenlei Zhang,Wenyu Tang,Xiao Yan,Yuru Zhang,Xiaolin Meng,Ronghua Lu,Guoxing Nie
Irisin, encoded by fibronectin type III domain-containing protein 5 (FNDC5) gene, plays a role in energy expenditure and insulin sensitivity in mice. In fish, the function of irisin related to glucose metabolism is less reported. It may increase glucose utilization in fish. The aim of the present study was to characterize the regulatory role of irisin in glucose metabolism in common carp ( Cyprinus carpio L.). In this study, FNDC5a and FNDC5b were isolated from common carp. The cDNA of FNDC5a and FNDC5b were 722 bp and 714 bp, encoding 221 and 207 amino acids, respectively. FNDC5a was abundantly expressed in the brain and gonad. FNDC5b was mainly expressed in brain. Different expression pattern of FNDC5a and FNDC5b under fasting/refeeding and OGTT experiment were identified. The recombinant common carp irisinA and irisinB were prepared by prokaryotic expression system. Glucose concentration was decreased in treatment with irisinA or irisinB in the in vitro and in vivo experiments. The mRNA expression levels of gluconeogenesis-related genes were significantly down-regulated, while the mRNA expression of glycolysis-related genes were significantly up-regulated after treatment with recombinant irisinA or irisinB in liver in vivo and in primary hepatocytes in vitro. Our research shows that irisin inhibits hepatic gluconeogenesis and promotes hepatic glycolysis. Taken together, this study for the first time revealed the two subtypes of FNDC5 and explored the function and mechanisms of irisinA and irisinB in fish glucose homeostasis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Mengting Zhu,You Long,Yingjie Ma,Yi Chen,Qiang Yu,Jianhua Xie,Bin Li,Jinlong Tian
This study aimed to quantitatively screen and compare the chemical compounds including protein, lipid, sucrose , total phenolics (TPC), and total titratable acidity (TTA) along with the fatty acid profile of green coffee beans from various geographical origins (China, Indonesia, Ethiopia, Kenya, Guatemala, Honduras, Brazil, and Colombia). Meanwhile, partial least squares discriminant analysis (PLS-DA) was used to investigate whether the analyzed parameters’ data can be successfully applied to differentiate the geographical origins of green coffee beans. The levels of protein, lipid, sucrose , TTA, and TPC in all samples were in the range of 13.06–15.98 g 100 g −1 , 12.88–16.29 g 100 g −1 , 7.30–11.45 g 100 g −1 , 231.43–361.21 mL 0.1 mol L −1 NaOH 100 g −1 , and 37.56–50.57 mg GAE g −1 , respectively. The fatty acid composition was dominated by C 18:2 , C 16:0 , C 18:1, and C 18:0 . The major fatty acid group was unsaturated fatty acids (USFA), accounting for 51.66–54.28% of total fatty acids. Statistical analysis showed significant differences in the chemical and fatty acid composition of green coffee beans from eight geographical origins ( P ＜ 0.05 ). Moreover, the PLS-DA results revealed excellent discrimination between the different geographical origins of green coffee beans, highlighting lipid, C 24:0 , C 22:0 , C 18:3 , C 17:0 , C 18:0 , C 20:0 , C 16:0 , and protein as discriminatory features.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jian Li,Debao Niu,Ying Zhang,Xin-An Zeng
The influences of different extraction methods on the yields, physicochemical properties, antioxidant activities and antiproliferative abilities of polysaccharides from Morinda citrifolia L. (Noni) were investigated and compared. Three extraction methods were applied to extract Noni polysaccharides (NP), which include hot water extraction (HWE), pulsed electric field-assisted extraction (PEFAE) and ultrasonic-assisted extraction (UAE). The extraction yields, sugar contents, uronic acid contents, molecular weights, monosaccharide proportions, antioxidant activities and antiproliferative abilities of three NP samples were significantly different. However, their preliminary structural characteristics and monosaccharide types were similar. More specifically, UAE-NP had the highest extraction yield, the smallest molecular weight and the best antioxidant activities. Especially, UAE-NP showed excellent antiproliferative abilities. Compared with HWE-NP and PEFAE-NP, the IC 50 value of UAE-NP on HepG2 cells reduced by 45.45% and 33.14%, respectively. The highest antioxidant activities of the UAE-NP might be attributed to its smaller molecular weight, lower Gal content and higher Fuc content. The strongest antiproliferative effects of the UAE-NP might be related to the low molecular weight. Overall, UAE could be a potential technique for the extraction of high-quality NP due to its high yield, high efficiency and notable bioactivities.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2020
·Vol. 77
·期 6
·1016-1023
·
IF 1.746
Yue Xue,Yan Bingyu,Wang Shuai,Gao Wen,Zhang Ruiyan,Dong Huijun
The glycopeptide A40926 biosynthesized by Nonomuraea gerenzanensis is a precursor of the second generation glycopeptide antibiotic dalbavancin. The skeleton of this glycopeptide consists of seven amino acids and is biosynthesized by the NRPS gene module. L-valine, a branched amino acid, is also a significant precursor for A40926 production. This study details the use of pH-responsive alginate–chitosan microspheres loaded with L-valine prepared by internal emulsification gelation. The effects of process and formulation variables on microsphere size, loading capacity, and encapsulation efficiency were investigated. Then, effects on A40926 production by the pH-responsive microspheres were evaluated in a 10-L fermenter. Results demonstrated that use of the pH-responsive microspheres could improve A40926 yield from 465 to 602 mg L −1 in a 10-L scale fermenter.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2020
·Vol. 75
·期 1
·111-118
·
IF 0.84
Congming Li,Zhou Yanli,Ye Baoxian,Xu Maotian
Abstract A novel, economic, and highly sensitive electrochemical sensor for the determination of trans -resveratrol ( RES ) was fabricated by electropolymerization of poly(L-lysine) films onto glassy carbon electrode ( GCE ) surface. The electrochemical behavior of RES in Britton–Robinson buffer solution (pH 2.0) on the poly(L-lysine)/GCE was investigated by cyclic voltammetry and linear sweep voltammetry for the first time. Results showed that the detection range of the proposed sensor to RES was from 0.20 to 12.0 μM with the minimum detectable concentration of 0.06 μM. The reported electrochemical sensor also exhibited high selectivity, good reproducibility, and long-term stability for RES detection. In addition, the proposed method can be employed for the determination of RES in red wines with satisfactory results. The proposed electrochemical strategy presents valuable information related to evaluation of RES levels in real samples.
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2021
·
IF 4.749
Han Sun,Jian Dong,Yangyufan Wang,Siyu Shen,Yong Shi,Lei Zhang,Jie Zhao,Xiaoliang Sun,Qing Jiang
The periosteum plays an important role in vascularization and ossification during bone repair. However, in most studies, an artificial periosteum cannot restore both functions of the periosteum concurrently. In this study, a novel nanofiber that can sustain the release of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) was fabricated to enhance the durability of angiogenesis and osteogenesis during bone regeneration. A cell-free tissue engineered periosteum based on an electrospinning poly-l-lactic acid (PLLA) nanofiber was fabricated, on which VEGF and BMP-2 were immobilized through a polydopamine (PDA) coating conveniently and safely (BVP@PLLA membrane). The results indicated a significantly improved loading rate as well as a slow and sustained release of VEGF and BMP-2 with the help of the PDA coating. BMP-2 immobilized on nanofibers successfully induced the osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) in vitro with high expression of runt-related transcription factor 2 (Runx2), osteopontin (OPN), and alkaline phosphatase (ALP). Similarly, angiogenic differentiation of BMSCs with the expression of fetal liver kinase-1 (Flk-1) and vascular endothelial cadherin (VE-cadherin) was observed under the environment of VEGF sustained release. Moreover, an in vivo study revealed that the BVP@PLLA membrane could enhance vascular formation and new bone formation, which accelerates bone regeneration in rat femoral defects along with a massive periosteum defect. Therefore, our study suggests that the novel artificial periosteum with dual growth factor controlled release is a promising system to improve bone regeneration in bone defects along with a massive periosteum defect.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Yu Zhang,Junxi Liu,Pengfei Dou,Zhijing Wu,Ziming Zheng,Xianglin Pan,Tao Zhou,Kaiping Wang
The elucidation of the oral absorption of natural polysaccharides contributes to their further research and utilization. Herein, to explore the absorption of a pectin-type polysaccharide from Smilax china L. (SCLP), SCLP was respectively fluorescently labeled with fluorescein-5-thioicarbazide (FSCLP) and Cyanine7 amine (Cy7-SCLP) for in vitro and in vivo tracking. The near-infrared imaging demonstrated that Cy7-SCLP was absorbable in the small intestine and distributed in the liver and kidney after oral administration. Subsequently, in vitro intestinal epithelial tissue experiments showed that the jejunum was the dominant site of FSCLP transport. Further transport studies in the Caco-2 cell monolayer illustrated that FSCLP was delivered across the monolayer via transcellular transport by caveolae-mediated endocytosis and macropinocytosis together with paracellular transport by reversibly affecting tight junctions. In summary, this work presents the oral absorption characteristics and mechanisms of SCLP through the intestinal epithelium, which will facilitate the further development of SCLP and pectin polysaccharides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Ruimin Wang,Ruiping He,Zhaohui Li,Songjie Li,Congfa Li,Lu Wang
Herein, high efficient extraction of natural antioxidants from partridge leaf-tea (PLT) was carried out by using eco-friendly choline chloride-based deep eutectic solvents combined with ultrasonic method. Choline chloride-malic acid (ChCl-MaA), a new type of green solvent, with the highest efficient for extracting the natural antioxidants from PLT (DPPH -756.70 ± 12.13 μmol TE/g DW; FRAP-4139.17 ± 65.79 μM Fe(Ⅱ)SE/g DW) was screened for further optimization. The key parameters for simultaneously maximizing total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhyldrazyl (DPPH ) and ferric reducing antioxidant activity (FRAP) were optimized by single-factor experiments along with response surface methodology (RSM). Results exhibited that L/S ratio and water content in DES had significant effects on TPC and antioxidant activities, while ultrasonic power indicated a significant negative effect on all responses. Under the optimal conditions (ultrasonic power at 210 W, ultrasonic time at 30 min, L/S ratio at 27.50:1, water content in DES at 45%), experimentally obtained results were obtained as follows: 42.20 ± 2.17 mg GAE/g DW for TPC, 30.33 ± 2.16 mg RE/g DW for TFC, 838.64 ± 12.81 μmol TE/g DW for DPPH , and 5958.33 ± 153.52 μM Fe(Ⅱ)SE/g DW for FRAP, which were well in agreement with the predicted values. Furthermore, ChCl-MaA-based ultrasonic assisted extraction (UAE) yielded higher TPC, TFC and antioxidant activities compared with traditional solvents-based UAE. Strong antioxidant activities of PLT extracts were correlated with high levels of individual antioxidants including salvianic acid A, 3-chlorogenic acid, gallic acid, catechin and rutin. The functional groups analysis by Fourier transform infrared spectroscopy (FT-IR) were used to compare the solvents characterization before and after extraction. Scanning electron microscope (SEM) results further verified ChCl-MaA intensified the destruction of cell wall structure, and thereby allowed greater release of antioxidants from PLT. This work provides an eco-friendly ChCl-MaA-based UAE technique for efficiently extraction and valorization of phenolic compounds from PLT, which can be further used in pharmaceutical, food, and cosmetic industries.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）