TALANTA
·
2024
·
IF 5.6
Zhao-He Chen,Nan Sun,Jian-Ping Li,Jia-Wei Zheng,Ya-Hao Wang,Xiao-Shun Zhou,Bin Zheng
As the extensive use of antibiotics has led to the rapid spread of antibiotic resistance, there is an urgent need for quantitative assessment of antibiotic residues in the environment. Surface-enhanced Raman spectroscopy (SERS) has emerged as a rapid and cost-effective detection method, but it suffers from the high variability in signal intensities, its quantitative detection remains challenging. Herein, we have developed a SERS calibration substrate with a silent region internal standard, enabling simultaneous and reliable quantitative detection of three commonly antibiotics of penicillin potassium (PP), tetracycline hydrochloride (TCH) and levofloxacin (LEV). The calibration substrate is made by assembling Au @ 4-mercaptobenzonitrile (4-MBN) @ SiO 2 on silicon wafer. The chemically-inert silica shell allows the substrate to remain SERS active for more than 24 weeks in the air. The v C ≡ N of 4-MBN in the silent region of 1800–2800 cm −1 provides an effective reference for correcting Raman signal fluctuations. The relative SERS intensity by normalizing to the internal standard (IS) of v C ≡ N shows a linear response against to the logarithmic concentration with a correlation coefficient of 0.997, 0.976, 0.998 and a limit of detection (LOD) of 26.9, 28.2, 2.4 nM for PP, TCH and LEV, respectively. Furthermore, simultaneous detection and principal component analysis (PCA) of these antibiotics in lake water with a concentration range of 1–100 mg/L can achieve a sensitivity and specificity of 100 %. This novel quantitative technique of using this SERS calibration substrate shows promises as a high-throughput platform for multiple trace antibiotics analysis.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Ying Li,Kang Liu,Xin Li,Xuan Jin,Jinze Lyu,Yuebing Xu,Haiyan Zhu,Gang Shi
It is difficult for metal-modified semiconductor nanotubes to obtain surface-enhanced Raman scattering (SERS) substrates through long-range ordered 3D assembly, which adversely affects both Raman signal enhancement and Raman signal uniformity. Here, ZnFe 2 O 4 nanotubes with Ag nanoparticles modified on the inner and outer walls are induced to grow on the surface of the ordered Si pyramid by ZnO nanonedles as sacrificial templates. In this way, the composite nanotubes (Ag/ZnFe 2 O 4 ) are assembled into dandelion flower-like 3D aggregates with a periodic arrangement of hexagonal close packing. This 3D ordered SERS substrate based on Ag/ZnFe 2 O 4 nanotubes has excellent anti-reflective performance and carrier separation ability, which is conducive to the enhancement of Raman signal. When the SERS substrate is used as a sensor, it can achieve the single molecule (10 −13 M) detection level of Rhodamine 6 G, the enhancement factor (EF) is 3.14 × 10 9 , and the linear fit (R 2 ) of quantitative analysis is as high as 0.998 from 10 −13 to 10 −4 M. Meanwhile, the long-range ordered arrays on the SERS substrate can ensure the signal uniformity of sensor, which relative standard deviation (RSD) is as low as 3.37 %. In addition, the photocatalytic self-cleaning capability of SERS substrate ensures that the sensor can be reused. Finally, the mechanism of the photogenerated carrier migration of Ag/ZnFe 2 O 4 and its contribution to Raman signal enhancement were analyzed.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-12
·
IF 5.3
Wu Wei,Han En,Ma Lixin,Zhou Ruiyun,Wang Chen,Bai Junwen,Cai Jianrong
A label-free surface-enhanced Raman scattering (SERS) sensor using optimal bimetallic Au@Ag nanocuboids (Au@Ag NCs) was developed for ultrasensitive detection of florfenicol residue in eggs. The Raman characteristic peaks of florfenicol detection were identified at 1145 cm −1 and 1595 cm −1 which were consistent with the theoretical Raman spectrum of florfenicol calculated by density functional theory. The Au@Ag NCs were optimized by changing the aspect ratio of Au nanorods and the thickness of Ag nanocuboids, achieving an enhancement factor of 1.69 × 10 6 for SERS signals. Under optimal conditions, the label-free SERS sensor demonstrated a broad linear range of 0.01 mg/kg to 100 mg/kg for florfenicol in real sample. The limit of detection of florfenicol in eggs was as low as 0.0410 μg/kg, and the recovery was 94.83% ~ 108.24%. Therefore, this label-free SERS sensor offers a rapid and sensitive approach for the trace detection of florfenicol residues in food safety monitoring. Graphical abstract
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Tao Gai,Jiaolai Jiang,Shaofei Wang,Yiming Ren,Shanli Yang,Zhen Qin,Lang Shao,Qian Wu,Jun Zhang,Junsheng Liao
Background In the fields of environmental monitoring and nuclear emergency, in order to obtain the relevant information of uranyl-induced environmental pollution and nuclear accident, it is necessary to establish a rapid quantitative analytical technique for uranyl ions. As a new promising technique, surface-enhanced Raman scattering (SERS) is hopeful to achieve this goal. However, uranyl ions are easily desorbed from SERS substrates under acidic conditions, and the structures of SERS substrates will be destroyed in the strong acidic aqueous solutions. Besides, the quantitative detection ability of SERS for uranyl ions needs to be promoted. Hence, it is necessary to develop new SERS substrates for accurate quantitative detection of trace uranyl in environmental water samples, especially in acidic solutions. Results In this work, we prepared silver ions/sodium alginate supramolecular hydrogel membrane (Ag + /SA SMH membrane), and the Ag + ions from the membrane were transformed into Ag/Ag 2 O complex nanoparticles under laser irradiation. The Raman signal of uranyl was strongly enhanced under the synergistic interaction of electromagnetic enhancement derived from the Ag nanoparticles and charge transfer enhancement between uranyl and Ag 2 O. Utilizing the peak of SA (550 cm −1 ) as an internal standard, a quantitative detection with a LOD of 6.7 × 10 −9 mol L −1 was achieved due to a good linear relation of uranyl concentrations from 1.0 × 10 −8 mol L −1 to 2 × 10 −6 mol L −1 . Furthermore, foreign metal ions hardly affected the SERS detection of uranyl, and the substrate could determine trace uranyl in natural water samples. Particularly, the acidity had no obvious effect on SERS signals of uranyl ions. Therefore, in addition to the detection of uranyl ions in natural water samples, the proposed strategy could also detect uranyl ions in strong acidic solutions. Significance and novelty A simple one-step method was used to prepare an Ag + /SA SMH membrane for rapid quantitative detection of uranyl ions for the first time. The proposed substrate successfully detected uranyl ions under acidic conditions by immobilizing uranyl ion in hydrogel structure. In comparison with the previous studies, a more accurate quantitative analysis for uranyl ions was achieved by using an internal standard, and the proposed strategy could determine trace uranyl in either natural water samples or strong acidic solutions.
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VIBRATIONAL SPECTROSCOPY
·
2024
·
IF 2.7
Fan Yang,Jing Zhao,Zhengang Wu,Yingna Wei,Hengyong Wei,Jingwu Li,Bo Li
Cocrystal is a new class of pharmaceutical crystal form, which could improve the physical and chemical properties of drug substance without affecting the internal structure of drug substance. Therefore, the cocrystals show a great deal of potential in the development and research of drugs. 5-Fluorouracil (5-FU) is one of the most widely used anti-pyrimidine drugs. However, its solubility is poor and its oral bioavailability is low. In this work, the cocrystal of 5-FU with nicotinamide was prepared by the solvent evaporation method. The cocrystal structure was confirmed by infrared spectroscopy (IR), X-ray diffraction (XRD) and Raman spectroscopy. The X-ray diffraction and infrared spectroscopy presented that there were significantly different peaks between the raw drug and the cocrystal. Raman spectral results also indicated the differences of such cocrystal compared with the raw drug. In addition, TiN-Ag composite SERS substrate was used to study the formation of 5-FU cocrystal drugs and the formation process was dynamically monitored, and the interaction between molecules during the cocrystal reaction was deeply understood. The cocrystal reaction process was tracked, it can be found that the peak intensity of the raw drug and cocrystal changes with the reaction, indicating that the TiN-Ag composite substrate can monitor the cocrystal transformation process.
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TALANTA
·
2024
·
IF 5.6
Bin Yang,Jia Liu,Xin Liu,Shiyue Niu,Fengming Zhang,Yuting Wang,Daqian Song,Shuyun Bi
The high efficient surface-enhanced Raman scatterring (SERS) methods to detect thiacloprid and imidacloprid were established using ZIF-8-wrapped Ag nanoparticles (AgNPs) modified with β -cyclodextrin ( β -CD). The substrate of ZIF-8/ β -CD@AgNPs was characterized by ultraviolet visible spectra (UV–vis), thermogravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). The interaction between the substrate and thiacloprid/imidacloprid was also explored. The optimum measurement conditions were obtained by response surface model based on single-factor experiments. Enhancement factors (EFs) of thiacloprid and imidacloprid were respectively 2.29 × 10 6 and 2.60 × 10 6 . A good linearity between the scattering intensity and the concentration of thiacloprid/imidacloprid within 3–1000 nmol L −1 /6–400 nmol L −1 was established. The interference experiments indicated that the methods had good selectivity . The SERS methods were successfully applied to detect thiacloprid and imidacloprid in several vegetables samples. The recoveries ranged from 95.5 % to 105 % (n = 5). The detection limits (LODs) (S/N = 3) for thiacloprid and imidacloprid were 1.50 and 0.83 nmol L −1 , respectively.
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ACS Sensors
·
2024
·
IF 8.2
Ying Xu,Zhiyu Li,Yuanrong Liao,Jun Wang,Tong Zhang,Xifu Liu,Yang Zhang
Noble metal nanoparticle (NMP)-based composite substrates have garnered significant attention as a highly promising technique for surface-enhanced Raman scattering (SERS) in diverse scientific disciplines because their remarkable ability to amplify and functionalize Raman signals has positioned them as valuable tools for molecular detection. However, optimizing the size and distribution of NMPs has not received sufficient emphasis because of challenges associated with the precise control of deposition and the modulation of reducing rates during growth. In this research, we achieved the optimized size and spatial patterns of AgNWs on electrospun poly(vinylidene fluoride) (PVDF) nanofibers by utilizing a polydopamine (PDA) layer as a mild and controllable reduction mediator, by which the size and density of the AgNWs could be relatively precisely manipulated, achieving a dense distribution of effective “hot spots”. On the other hand, harnessing the inherent piezoelectric properties of the electrospun PVDF nanofibers further boosted the LSPR effect during the SERS test, forming a flexible dual-enhancing composite SERS substrate with excellent sensitivity. In addition to addressing structural aspects, exploiting synergistic systems capable of transferring external energy or forces to enhance the SERS performances presents a compelling avenue to broaden the practical applications of SERS. The dual-enhanced substrate achieved an exceptional enhancement factor (EF) of 1.05 × 108 and a low detection limit (LOD) of 10–10 M during the SERS test. This study focuses on integrating NMPs with electrospun piezoelectric polymer nanofibers to develop a dual-enhancing SERS substrate with excellent sensitivity and practicality. The findings provide valuable insights into controllably depositing NMPs on electrospun polymer fibers and hold significant implications for the development of highly sensitive and practical SERS substrates across various applications.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Houyang Ge,Qizhen Chen,Sheng Chen,Cong Li,Xingen Gao,Wanqiang Fu,Hongyi Zhang,Fuqiang Wang,Xianqiong Gong,Junzheng Wu,Juqiang Lin
Plasma-free amino acid (PFAA) profiling is crucial for monitoring and evaluating cancer progression. However, there remains a lack of sensitive, simple, and direct methods for rapid PFAA detection. In this study, we developed a vapor-deposited 3D flexible porous structure named FPS Ag - Coated serving as a high-performance surface-enhanced Raman spectroscopy (SERS) platform for ultrasensitive detection of micro PFAAs (10 −4 M). The surface of the platform contains abundant hydroxyl functional groups, which not only confer exceptional flexibility but also significantly minimize background signal interference. The 3D FPS architecture provides densely packed hotspots for Raman signal enhancement while demonstrating excellent cost-effectiveness and scalability for mass production. We systematically evaluated the platform's detection capability using four representative amino acids: fluorescent amino acids (L-tyrosine and L-tryptophan), weakly fluorescent amino acid (L-phenylalanine), and non-fluorescent amino acid (L-serine). Across a concentration range of 10 −1 to 10 −4 M, the platform effectively measured characteristic SERS signals with linear correlation, enabling real-time monitoring and quantitative analysis of PFAAs. When applied to clinical plasma samples from cancer patients, the platform successfully identified PFAAs-specific SERS signal. Subsequent principal component analysis (PCA) achieved discrimination between different cancer types and healthy volunteers with an accuracy exceeding 83.3 %. This work presents a high-performance SERS platform for PFAA recognition, offering a highly adaptable flexible material that could facilitate the clinical translation of SERS-based diagnostics.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Qingling Nie,Ru Liu,Jiajia Liu,Yirong Xie,Yeting Tu,Ruiyun You,Yudong Lu,ChengLu Li,Zhenhua Liu,Jingbo Chen
Accurate detection of hydrogen sulfide (H₂S) in live cells is critical for understanding its role in disease but is challenged by poor biocompatibility, signal instability, and background interference. Here, we developed a novel surface-enhanced Raman scattering (SERS) hydrogel platform based on carboxymethyl cellulose‑silver nanoparticles embedded in agarose (CMC-Ag NPs-AG). This platform is prepared via simple physical crosslinking, enabling uniform Ag NP dispersion and stable encapsulation.The hydrogel shows excellent swelling capacity, antibacterial activity (>99 % inhibition), and high biocompatibility (>98 % cell viability). Leveraging the specific formation of Ag₂S upon H₂S exposure, the platform enables ultrasensitive and selective detection. It exhibits a wide linear range (0.15–600 μM), a low detection limit of 0.15 μM, and excellent reproducibility (RSD = 1.10 %). It also demonstrates strong anti-interference performance against structurally similar sulfur-containing molecules.Furthermore, this platform was successfully applied to monitor endogenous H₂S in A549 cancer cells under varying culture conditions. The results confirm its potential for real-time, in situ detection of gaseous signaling molecules in complex biological environments. This work offers a robust and biocompatible SERS strategy for H₂S analysis and provides new insights into tumor metabolism and disease diagnosis.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Ningtao Cheng,Yuling Deng,Xinmei Cao,Yi Tao
Foodborne pathogens significantly impact public health and cause substantial economic losses globally, necessitating rapid, sensitive, and multiplex detection techniques. This study presents a novel surface-enhanced Raman spectroscopy (SERS) biosensor enabling highly sensitive and simultaneous detection of Salmonella enteritidis ( S. enteritidis ) and Pseudomonas aeruginosa ( P. aeruginosa ). The biosensor utilizes sculpted triangular silicon substrates (stSi) decorated uniformly with silver (Ag) and gold (Au) nanoparticles (stSi@Ag-Au), fabricated through electrochemical reduction and galvanic displacement methods, creating robust electromagnetic hotspots. Functional gold nanostars synthesized via a seed-mediated growth method were deposited onto stSi@Ag-Au, forming a Raman-active substrate, which was further modified with pathogen-specific aptamers and distinct Raman probes—5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) for S. enteritidis and 4-(mercaptomethyl)benzonitrile (MMBN) for P. aeruginosa . These components assemble into supramolecular complexes capable of specifically recognizing target pathogens, generating intense, unique Raman signals in a concentration-dependent manner, enabling concurrent quantitative analysis of both pathogens. The developed method achieves rapid detection within 45 min, demonstrates a broad linear detection range (10°–10 5 CFU mL −1 ) with detection limits of 1.33 CFU mL −1 ( Salmonella enteritidis ) and 1.12 CFU mL −1 ( Pseudomonas aeruginosa ), and has been successfully validated in practical applications in diverse matrices, including drinking water, orange juice, and Dendrobium nobile Lindl. juice samples, underscoring its effectiveness for rapid food safety assessments. Overall, this supramolecular-based SERS biosensing strategy provides a highly sensitive, rapid, and reliable solution for simultaneous pathogen detection, significantly advancing capabilities in foodborne disease prevention and control.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jihua Xu,Chengrui Zhang,Ziluo Chen,Xiaofei Zhao,Zhen Li,Jing Yu,Chao Zhang,Yuzhi Song,Mingrui Shao
There is an immense potential for surface-enhanced Raman scattering (SERS) sensors in realizing the monitoring of poison gas. However, conventional SERS detection techniques are difficult to achieve omnidirectional detection, resulting in poor signals under large-angle laser excitation. Herein, urchin-shaped manganese dioxide (MnO 2 ) microspheres were grown directly in the flexible polyvinylidene fluoride (PVDF) cavity (PC) to obtain PC/MnO 2 (PCM). The PCM was modified by silver nanoparticles (Ag NPs) and zeolite imidazole framework-8 (ZIF-8) to form the PCM/Ag/ZIF-8 SERS sensor. In addition to enriching the target molecules and protecting the internal Ag NPs, ZIF-8 nanoparticles are able to modulate the distribution of “hot spots” on the spines of urchin-shaped MnO 2 . This modulation ensures the reliability of the PCM/Ag/ZIF-8 sensor by maintaining excellent SERS detection performance even under large-angle laser excitation. Furthermore, the unique urchin-shaped structure enables the gas to form vortex between neighboring spines, which greatly slows down the airflow and improves the contact time between the gas molecules and the sensor, thus achieving effective enrichment of the gas molecules. Experimental results show that the PCM/Ag/ZIF-8 sensor is capable of detecting formaldehyde gas at concentrations as low as 1 ppb. This work provides an innovative approach for reliable detection of toxic gases in practical applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dazhuang Liu,Shuna Mi,Shaofeng Yuan,Hang Yu,Yahui Guo,Yuliang Cheng,Weirong Yao
This study developed a human serum albumin-functionalized gold core-shell nanocomposite (Au@HSA) to address limitations of commercial AuNPs in detecting sodium pentachlorophenol (NaPCP) and ibuprofen (IBU) via surface-enhanced Raman scattering (SERS). Molecular docking and multispectral analyses revealed HSA-induced structural transitions (α-helix to β-sheet) enhancing substrate stability and analyte adsorption. Optimized detection parameters (HSA: 1 μg/mL; pH 7 for NaPCP, pH 9 for IBU; 30–55 min incubation; Au@HSA-to-analyte ratios 2:1–5:2) enabled linear detection of both analytes (0.5–100 mg/L) with limits of 0.32 mg/L (NaPCP) and 0.27 mg/L (IBU). Validations in tap water and pears demonstrated 97.10 %–102.84 % recovery rates and ≤ 4.64 % RSD, confirming practical applicability. The Au@HSA platform overcomes commercial AuNP limitations by leveraging HSA's molecular recognition and stabilization properties, offering enhanced sensitivity for dual-pollutant monitoring in environmental and food samples. This work provides a robust solution for detecting toxic pesticides and pharmaceutical pollutants while advancing functional nanocomposite design for SERS-based sensing.
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