PLANT FOODS FOR HUMAN NUTRITION
·
2023
·Vol. 78
·期 2
·390-398
·
IF 4
Jing Yongshuai,Su Ziteng,Zhang Shilin,Han Qiyuan,Wang Ziying,Hu Beibei,Zhang Danshen,Sun Shiguo,Wu Lanfang
An acidic polysaccharide (SMP) with a molecular weight (Mw) of 1.28 × 10 6 Da was isolated from Salvia miltiorrhiza. The monosaccharide composition in molar percentages was rhamnose (Rha): galacturonic acid (GalA): galactose (Gal): arabinose (Ara) = 6.15: 55.98: 21.27: 16.69. The results of simulated digestion in vitro showed that SMP was not degraded in saliva, gastric juice or intestinal juice. The Y maze test and new object recognition test showed that SMP could improve the working memory impairment of aging mice. SMP could also increase the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and brain tissue, decrease the content of malondialdehyde (MDA), decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in brain tissue, and increase the content of short-chain fatty acids (SCFA) in the intestine. In addition, SMP could also regulate the intestinal flora structure, including increasing the relative abundance of Firmicutes and Bacteroidetes and decreasing the relative abundance of Proteobacteria . This work lays a foundation for the development of functional foods related to Salvia miltiorrhiza .
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 6
·1-9
·
IF 5.7
Zeng Yinan,Zhang Min,Peng Kefu,Man Zu,Guo Lu’an,Liu Wenping,Xie Shilei,Liu Peng,Xie Dong,Wang Shoushan,Cheng Faliang
A photoelectrochemical molecular imprinting sensor based on Au/TiO 2 nanocomposite was constructed for the detection of dibutyl phthalate. Firstly, TiO 2 nanorods were grown on fluorine-doped tin oxide substrate by hydrothermal method. Then, gold nanoparticles were electrodeposited on TiO 2 to fabricate Au/TiO 2 . Finally, molecular imprinted polymer was electropolymerized on the Au/TiO 2 surface to construct MIP/Au/TiO 2 PEC sensor for DBP. The conjugation effect of MIP accelerates the electron transfer between TiO 2 and MIP, which can greatly improve the photoelectric conversion efficiency and sensitivity of the sensor. In addition, MIP can also provide sites for highly selective recognition of dibutyl phthalate molecules. Under optimal experimental conditions, the prepared photoelectrochemical sensor was used for the quantitative determination of DBP and the results showed a wide linear range (50 to 500 nM), a low limit of detection (0.698 nM), and good selectivity. The sensor was used in a study of real water samples to show that it has promising applications in environmental analysis. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 17
·3581-3592
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IF 4.3
Zhou Qian,Su XiaoLu,Yao YanXing,He Bin,Jin FengMei,Gao MengYue,Wang Qi,Feng GuiPing,Li BaoXi,Liu Rui,Dong Jun
Enrichment for the detection of quinolone residues is usually cumbersome and requires large amounts of toxic organic reagents. Therefore, this study synthesized a low-toxicity hydrophobic deep eutectic solvent (DES) with dl -menthol and p-cresol, which was then characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and thermal analysis. A simple and rapid vortex-assisted liquid–liquid microextraction method was developed based on this DES for the extraction of eight quinolones from cattle urine. The optimal extraction conditions were screened by examining the DES volume, extraction temperature, vortex time, and salt concentration. Under the optimal conditions, the linear ranges of the eight quinolones were 1 ~ 100 μg/L with good linearity ( r 2 was 0.998 ~ 0.999), and the limits of detection and quantification were 0.08 ~ 0.30 μg/L and 0.27 ~ 0.98 μg/L, respectively. The average extraction recoveries of spiked cattle urine samples were 70.13 ~ 98.50% with relative standard deviations below 13.97%. This method can provide a reference for the pre-treatment of quinolone residue detection. Graphical Abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Liu Jianming,Zhang Han,Xu Yingying,Meng Hao,Zeng An-Ping
The use of gaseous and air-captured CO 2 for technical biosynthesis is highly desired, but elusive so far due to several obstacles including high energy (ATP, NADPH) demand, low thermodynamic driving force and limited biosynthesis rate. Here, we present an ATP and NAD(P)H-free chemoenzymatic system for amino acid and pyruvate biosynthesis by coupling methanol with CO 2 . It relies on a re-engineered glycine cleavage system with the NAD(P)H-dependent L protein replaced by biocompatible chemical reduction of protein H with dithiothreitol. The latter provides a higher thermodynamic driving force, determines the reaction direction, and avoids protein polymerization of the rate-limiting enzyme carboxylase. Engineering of H protein to effectively release the lipoamide arm from a protected state further enhanced the system performance, achieving the synthesis of glycine, serine and pyruvate at g/L level from methanol and air-captured CO 2 . This work opens up the door for biosynthesis of amino acids and derived products from air.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·
IF 5
Si Zhenyuan,Cai Yang,Zhao Lang,Han Lu,Wang Feng,Yang Xiaobing,Gao Xiangdong,Lu Meiling,Liu Wei
Abstract α-L-Arabinofuranosidases (Abfs) play a crucial role in the degradation of hemicelluloses, especially arabinoxylans (AX). Most of the available characterized Abfs are from bacteria, while fungi, as natural decomposers, contain Abfs with little attention given. An arabinofuranosidase ( Th Abf1), belonging to the glycoside hydrolase 51 (GH51) family, from the genome of the white-rot fungus Trametes hirsuta , was recombinantly expressed, characterized, and functionally determined. The general biochemical properties showed that the optimal conditions for Th Abf1 were pH 6.0 and 50°C. In substrate kinetics assays, Th Abf1 preferred small fragment arabinoxylo-oligosaccharides (AXOS) and could surprisingly hydrolyze di-substituted 2 3 ,3 3 -di-L-arabinofuranosyl-xylotriose (A 2,3 XX). It also synergized with commercial xylanase (XYL) and increased the saccharification efficiency of arabinoxylan. The crystal structure of Th Abf1 indicated the presence of an adjacent cavity next to the catalytic pocket which led to the ability of Th Abf1 to degrade di-substituted AXOS. The narrow binding pocket prevents Th Abf1 from binding larger substrates. These findings have strengthened our understanding of the catalytic mechanism of GH51 family Abfs and provided a theoretical foundation for the development of more efficient and versatile Abfs to accelerate the degradation and biotransformation of hemicellulose in biomass. Key points ThAbf1 from Trametes hirsuta degraded di-substituted arabinoxylo-oligosaccharide. ThAbf1 performed detailed biochemical characterization and kinetics. ThAbf1 structure has been obtained to illustrate the substrate specificity. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 36
·85558-85568
·
IF 5.8
Jin
Jingnan,Shi Yahui,Zhang Baozhong,Wan Dongjin,Zhang Qingye
A bacterial strain Citrobacter sp. HJS-1 was discovered from the sludge in a drainage canal of a coal mine. Firstly, its biodegradation capacity for benzo[a]pyrene (BaP) was detected under different concentrations. The results proved that the strain possessed excellent biodegradation capacity for BaP with high-efficiency degradation rates ranging from 78.9 to 86.8%. The highest degradation rate was observed in the low-concentration sample, and the high-concentration BaP had a slight influence on the biodegradation capacity due to the potential toxicity of BaP and its oxygen-containing derivatives. Meanwhile, the degradation test for the other five aromatic hydrocarbons (2- to 4-ring) proved that the strain had a comprehensive degradation potential. To clarify the biodegradation mechanism of BaP, a dioxygenase structure was constructed by homology modeling. Then, the interactions between dioxygenase and BaP were researched by molecular simulation. Combined with the identification of the vital BaP-cis-7,8-dihydrodiol intermediate and the interaction analysis, the initial oxidation mode and the binding site of BaP were revealed in the dioxygenase. Taken together, this study has offered a way to understand the biodegradation process of BaP and its interaction mechanism based on experimental and theoretical analysis.
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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2023
·
IF 2.3
Qin Qin,Cao Weiqi,Zhaxi DaWa,Chen Xianyong,Andreeva Daria V.,Chen Kefan,Yang Shuai,Tian Hao,Shaker Majid,Jin Zhan,Novoselov Kostya S.
Phase change materials (PCMs) offer efficient solutions for energy sustainability. Here, we used 1-decanol, expanded graphite (EG) with high thermal conductivity, and silica aerogel (SA), which is often used for thermal insulation protection, to prepare composite PCMs (EG-PCM and SA-PCM) with stable shape through the vacuum absorption method. The leakage rate test after heat treatment determined the optimum contents of EG and SA in the composite PCMs to be 9 and 14 wt.%, respectively. Even after 220 heating/cooling cycles, the fabricated composite PCMs still maintain good thermal stability. The thermal conductivity enhancement of EG-PCM and SA-PCM to 16.09 times and 1.21 times of the neat PCM, respectively. Moreover, we tested the prepared two composite PCMs by yogurt preservation experiments in a cold chain transport portable box, which proved that they could effectively maintain the temperature and acidity of yogurt for several hours without any cooling system. Based on these results, EG-PCM and SA-PCM have broad application prospects in cold chain transport of temperature-sensitive products such as food, medicine, and vaccine.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 16
·1-11
·
IF 2.8
Peng Haiyi,Wang Feifei,Lin Huixing,Yao Xiaogang,Zeng Huarong
In recent years, pressure sensors with high sensitivity and flexibility have been widely used in the fields of wearable electronics, soft robots, medical monitoring, and so on. In this paper, a through-hole structure comprising of multi-walled carbon nanotubes (MwCNTs)/carboxymethyl cellulose (CMC)/polydimethylsiloxane (PDMS) was constructed through an ice template method and then it functioned as the dielectric layer of a newly made flexible capacitive pressure sensor. The sensitivity, stability, response time, and human applicability of the sensor were fully studied. The experimental method with ice template has the advantages of environmental protection and safety. More importantly, through the regulation of chemical composition, the porous sponge can have a specific pore morphology. The experimental e results showed that the dielectric layer with a through-hole structure had been built successfully and the PDMS resin was impregnated into the through-hole structure. The MwCNTs/CMC/PDMS capacitive pressure sensor possessed a high sensitivity (~ 2.143 kPa −1 ) and good stability in the low-pressure range (0–1 kPa). Moreover, a low detection limit (~ 3 Pa) and fast response time (~ 250 ms) were obtained. Human applicability tests revealed that our flexible sensor could catch the response of the human body swiftly so that it exhibited bright application prospects in wearable electronics.
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IONICS
·
2023
·Vol. 29
·期 8
·3407-3419
·
IF 2.8
Han Jiale,Liu Yunhang,Li Fang,Zhu Gan,Guo Meimei,Zhao Hongyuan
Herein, we reported an efficient ultrasound-assisted method to synthesize the nanocomposite of graphitized and carboxylated multi-walled carbon nanotubes and chitosan (GCMCN@CTS), which was used to fabricate the GCMCN@CTS/GCE sensor for the detection of niclosamide (NA). GCMCN with graphitization and carboxylation possessed excellent electrical conductivity and high specific surface area. Graphitization enhanced the electrical conductivity of carbon nanotubes, and carboxylation improved the dispersion degree of carbon nanotubes. CTS with good biocompatibility possessed strong adsorption ability and superior film-forming property. Moreover, the introduction of CTS facilitated the uniform dispersion of GCMCN, and GCMCN with excellent electrical conductivity could make up for the poor electrical conductivity of CTS. Benefitting from the functional collaboration of GCMCN and CTS, an ultra-low detection limit of 1.4 nM could be achieved at the fabricated GCMCN@CTS/GCE sensor in linear NA concentration range of 0.01–10 μM. Moreover, the proposed sensor showed satisfactory reproducibility, repeatability, and anti-interference performance. When used for NA detection in tap water and lake water samples, the GCMCN@CTS/GCE sensor exhibited gratifying recoveries of 96.0–103.8% with RSD values of 2.0–4.7%.
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AAPS PHARMSCITECH
·
2023
·Vol. 24
·期 6
·1-10
·
IF 3.3
Tang XiaoFei,Qin Huaiying,Yang Jianhua,Zhang XiaoYun
Dissolving microneedle (DMN) has been researched as a drug delivery technology that improves drug molecule transportation through the skin with little discomfort. However, the sluggish drug absorption, poor skin dissolution, and lengthy time lags of DMN have limited its potential uses. The aim of this study was to design a novel DMN system for the administration of the poorly water-soluble drug, estradiol (E2), with fast skin penetration and a stable release rate for a long time. DMN containing E2 emulsion (E2-EM-DMN) and traditional DMN (T-DMN) were prepared. Rat skin was used for penetration test and guinea pig skin was used for skin irritation experiment. The drug release profiles and stability properties of these two kinds of DMNs were also investigated. High performance liquid chromatography was employed to determine the E2 content in DMN. The E2 concentration in rat plasma was achieved by a newly developed liquid chromatography-mass spectrometry method that was fast, reproducible, and specific. The height of E2-EM-DMN and T-DMN was 600 μm. The drug loading of the E2-EM-DMN and T-DMN was 667.30 ± 7.21 μg/patch and 672.56 ± 6.98 μg/patch. E2-EM-DMN possessed enough mechanical strength to penetrate the skin and caused no irritation to the skin. E2-EM-DMN could release the drug more rapidly and more continuously than T-DMN. E2-EM-DMN had good pharmaceutical stability. In summary, the E2-EM-DMN showed reliable quality and superior release performance. Emulsion-embedded DMN is an ideal transdermal delivery system for drugs. Graphical Abstract
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Journal of Food Measurement and Characterization
·
2023
·
IF 3.4
Zhang Wenqi,Zhang Zhihua,Zhang Zhirui,Ling Jiang,Ding Yanjun
Histamine (HA) plays an important role in many pathological processes including asthma, diarrhea, and anaphylactic shock. Therefore, accurate and rapid detecting HA is of great significance. In this work, we report on a novel turn-on fluorescent aptasensor for HA detection based on a competitive combination process performed by both HA-specific and HA-analogs of aptamers. The analogue of HA was environmental-friendly obtained on the polymer chains of carbonized polymer dots (CPDs) without complex functionalization, in which the histidine was used as the polymer precursors for the first time (His-CPDs). After modification with aptamer, the analogue of HA on the His-CPDs would combine with the aptamer, leading to the agglomeration of His-CPDs and the fluorescence quenching. In the presence of HA, the HA would compete with the analogue of HA to react with the aptamer, leading to the dissociation of the agglomerates and the recovery of fluorescence. Under optimized conditions, the proposed fluorescent aptasensor can sensitively detect HA in 7 min ranging from 50 ng/mL to 40 µg/mL with the limit of detection (LOD) of 30 ng/mL. More importantly, this proposed fluorescent aptasensor can sensitively detect histamine in human serum and real sardine samples without complex pre-processing, showing great potential in the medical diagnosis of histamine intoxication and anaphylactic shock.
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CATALYSIS LETTERS
·
2023
·
IF 2.8
Li Zezhong,Xu Jing,Liu Zhenlu,Liu Xinyu,Xu Shengming,Ma Yue,Li Qian,Shang Yan
The p–n type Sm 2 WO 6 -Co 9 S 8 heterojunction with two-dimensional coupling interface was successfully synthesized by electrostatic self-assembly. The unique rhombic morphology of Sm 2 WO 6 and Co 9 S 8 nanoparticles also effectively improve the light utilization efficiency. The intervention of Co 9 S 8 enhances the visible light absorption capacity of m 2 WO 6 /Co 9 S 8 . The Sm 2 WO 6 /Co 9 S 8 greatly increases the specific surface area and provides a rich reaction site. Recombination of photogenerated carriers in Sm 2 WO 6 /Co 9 S 8 heterojunctions is effectively inhibited. The p–n type Sm 2 WO 6 -Co 9 S 8 heterojunction results show that the optimized Sm 2 WO 6 /Co 9 S 8 heterojunction exhibits significant hydrogen evolution activity, and the optimal Sm 2 WO 6 /Co 9 S 8 -25 composite has 117.5 μmol hydrogen evolution activity, it’s close to four times Co 9 S 8 , close to 143 times Sm 2 WO 6 . The stability of the composite Sm 2 WO 6 /Co 9 S 8 is the better. Under the built-in electric field induced by p–n heterojunction, using of p–n heterojunction interactions and electron transport modes, the Sm 2 WO 6 /Co 9 S 8 can effectively achieve the remarkable effect of its internal carrier separation. This work also provides new opportunities for studying rare earth element tungstate photocatalysts. Graphical Abstract
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