Ning Zhang,Yingxia Guo,Yijin Wang,Luyao Yu,Chunpeng Feng,Jiye Tian,Lei Yin,Meiyun Shi
Revealing the potential of cellular cytotoxic and pharmacokinetics for methoxy‑polyethylene glycol propionic acid (M-PEG-PA) polymers is critical for evaluating the biocompatibility of PEG-based nanocarriers. M-PEG 6 -PA was employed as a target compound in this study. Cellular uptake of M-PEG 6 -PA polymers was first investigated by a green and selective ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) assay. After micro-solid phase extraction (μ-SPE), M-PEG 6 -PA and internal standard (IS) M-PEG 5 -PA were separated on an ACQUITY UPLC® BEH C 18 (2.1 × 50 mm I.D, 1.7 μm) chromatographic column. Multiple reaction monitoring (MRM) of characteristic transitions at mass-to-charge ratio ( m/z ) 367.2 [M-H] - → 118.8 and m/z 323.2 [M-H] - → 75.1 were selected for quantification of M-PEG 6 -PA and M-PEG 5 -PA polymers, respectively. The established analytical method for quantitative analysis of M-PEG 6 -PA polymer demonstrated strong linear correlation within 0.01 to 1 μg/mL. This validated assay was subsequently applied to investigate cellular internalization of M-PEG 6 -PA in MCF-7 cell samples. The experimental results revealed minimal cytotoxic effects of M-PEG 6 -PA at exposure levels spanning 3 μg/mL to 3 mg/mL, with intracellular accumulation remaining below 0.03 % of administered doses in MCF-7 cell samples.
Temperature and pH double-response poly(t-butyl acrylate)- b -poly{[2-(dimethylamino) ethyl methacrylate]- co -[poly(ethylene glycol) methyl ether methacrylate]} (PtBA -b- P(DMAEMA- co -PEGMEMA)) were successfully prepared by RAFT polymerization and could be self-assembled into micelles . 1 H NMR, UV-vis absorbance and DLS measurements were used to characterize their structures and self-assembly behaviors. At the same time, the adsorption of organic dyes and the control of drug release were also introduced. The results showed that the critical micelle concentration of PtBA- b -P(DMAEMA- co -PEGMEMA) was 2.55 mg/L. As the value of pH increased from 2 to 11, their particle size decreased from 178 nm to 130 nm. PtBA- b -P(DMAEMA- co -PEGMEMA) was also temperature responsive, with the hydrophobic block grows, the temperature of the particle mutation would increase. Therefore, PtBA- b -P (DMAEMA- co -PEGMEMA) could control the transfer and release of organic dyes such as methyl red and dithizone through their pH-sensitive properties, and the process had good reversibility. Meanwhile, PtBA- b -P(DMAEMA- co -PEGMEMA) micelles could also deliver some specific hydrophobic anticancer drug , such as camptothecin (CPT). In conclusion, the copolymers synthesized in this work have potential applications in the fields of environmental protection and drug delivery.
危险品化学品经营许可证（带存储）