Food Frontiers
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2024
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IF 7.4
Ziyi Han,Leyu Wang,Panjie Sun,Mengxin Huang,Fei Yu,Junsheng Liu,Yuanyuan Wu,Puming He,Youying Tu,Bo Li
Hyperglycemia and diabetes are common metabolic disorders. It is considered a safe and effective strategy to screen active ingredients from food and herbs for controlling blood sugar levels. Quinic acid (QA) is a natural polyphenolic acid with various health-promoting properties. In this study, QA was found to exhibit a potent inhibitory effect on α-glucosidase activity, with a half maximal inhibitory concentration (IC 50 ) of 5.46 mM. This inhibitory property surpassed that of three common organic acids including gallic acid, malic acid, and citric acid. A combination of 25% acarbose (0.5 mM) and 75% QA (5 mM) (v/v) exhibited synergistic inhibition of α-glucosidase activity. Enzyme kinetics, fluorescence spectra, and molecular docking analyses indicated that QA acted as an uncompetitive inhibitor of α-glucosidase, with hydrogen bonds playing a key role in the intermolecular interactions. Moreover, QA was found to effectively inhibit three major nonenzymatic glycation products including advanced glycosylation end products (AGEs), fructosamine, and α-dicarbonyl in a dose-dependent manner, outperforming the positive control aminoguanidine (AG) within the tested concentration range. Utilizing a Caco-2 cell model, QA demonstrated the ability to inhibit the transmembrane absorption of glucose. This study highlighted QA as a promising food functional factor that had been overlooked in the past, offering potential benefits in improving hyperglycemia, diabetes, and associated complications through the inhibition of α-glucosidase, nonenzymatic glycosylation, and glucose uptake.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Wenwen Pei,Xuewen Zhang,Yan Zeng,Jiao Li,Ziyi Li,Jiangang Yang,Xinjun Du,Yueming Zhu,Yuanxia Sun
α-Hydroxyketones, which have significant industrial applications, can be sustainably synthesized through the oxidation of secondary alcohols using secondary alcohol dehydrogenase (SADH). However, the activity of the SADH oxidation reaction is generally low, making it unsuitable for large-scale production. In this study, a rational design approach was employed to computationally engineer SADH derived from Ogataea parapolymorpha ( Op SADH), significantly enhancing its oxidation activity towards ( R )-1,2-propanediol (PDO). The mutant M2 (S222T/S316A) exhibited a 14.2-fold increase in specific enzyme activity compared to the wild type (WT) and was employed as a catalyst for high-concentration hydroxyacetone production, facilitated by an NAD + regeneration system. By applying an appropriate Zn 2+ ion force field in molecular dynamics (MD) simulations, it was found that two mutation sites could stabilize the conformations of NAD + and PDO, thereby revealing the molecular mechanism behind the enhanced activity of this metalloenzyme mutant. Notably, we first uncovered the dynamic mechanism by which four key residues (Cys39, His75, Glu76, and Glu175) and PDO within the active pocket contribute to the formation of coordination bonds with Zn 2+ . The findings of this study provide robust support for researching the catalytic mechanisms and dynamics processes of metalloenzymes.
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yingxin Wu,Kainan Hong,Juzhen Yi,Juncheng Shen,Liqun Yang
Surface-enhanced Raman spectroscopy (SERS) shows unique advantages for the study of protein structure in water environment, because it can provide plenty of structural information of proteins in addition to very weak Raman scattering signal of water. To achieve the Raman-enhanced effect of proteins, the challenge is to prompt protein molecules to approach the surface of noble metal (such as Ag and Au). Based on the enzyme–substrate specificity, the Ag nanoparticles-encapsulated amylopectin (Amyp/AgNPs) colloid was prepared as an aqueous SERS substrate to obtain the Raman-enhanced effect of amylase. Dynamic light scattering analysis showed that the hydrodynamic diameter of the Amyp/AgNPs colloid was in the range of 40–700 nm. UV–vis spectroscopy and energy dispersive spectroscopy confirmed the formation of Ag nanoparticles in the Amyp/AgNPs colloid. The Ag nanoparticles in the Amyp/AgNPs colloid were observed as globular homogeneous particles in the sizes ranging from 10 to 20 nm using transmission electron microscopy (TEM). The crystalline Ag nanoparticles were further observed in the high-resolution TEM image. The obviously enhanced Raman bands assigned to the amino acid residues and the secondary structures of α -amylase and β -amylase appeared in their SERS spectra, which were further analyzed following the principal component analysis (PCA) process. The Raman-enhanced mechanism is considered as that the specificity of two amylases with amylopectin in the Amyp/AgNPs colloid may prompt them to approach the surface of Ag nanoparticles and then produce the significant SERS effect. The SERS strategy based on enzyme–substrate specificity is thus anticipated to apply for structural analysis of enzyme.
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PubMed
高影响因子
JOURNAL OF CATALYSIS
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2024
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IF 6.5
Peng Zhang,Fangfang Li,Xuefang Yao,Pengze Zhang,Yukai Wu,Mengmeng Xi,Fanhui Meng,Lihua Kang,Mingyuan Zhu
The GaZrO x solid solution has been recently employed in OX-ZEO bifunctional catalysts for efficient conversion of syngas to light olefins, but it remains challenging to identify the specific contributions of Zr and Ga and to understand the detailed reaction mechanisms due to the structural complexity. To address this complexity and elucidate the active sites over the Zr-Ga oxide system, this study presents a catalyst designed by physical mixing ZrO 2 with different crystalline phases ( m -ZrO 2 & t -ZrO 2 ) and α -Ga 2 O 3 . The physical mixture of m -ZrO 2 and α -Ga 2 O 3 achieves a high CO conversion of 39.1 %, with light olefins selectivity of 87.3% among hydrocarbons after combined with SAPO-34, surpassing the performance of physical mixture of t -ZrO 2 and/or α -Ga 2 O 3 with SAPO-34. Combining the various characterizations and DFT calculations, we have confirmed that m -ZrO 2 site, enriched with oxygen vacancies and surface hydroxyl groups, is essential for CO adsorption and HCOO* intermediate formation, while the α -Ga 2 O 3 site plays a key role in the dissociation of H 2 . Furthermore, the electron transfer from m -ZrO 2 to α -Ga 2 O 3 in their mixture is more efficient than in a physically mixed t -ZrO 2 and α -Ga 2 O 3 . This accelerates the H 2 dissociation and boosts the spillover of hydrogen from α -Ga 2 O 3 to m -ZrO 2 , leading to the formation of more CH 3 O* intermediates and higher light olefins yield when combined with SAPO-34.
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CHEMICAL ENGINEERING SCIENCE
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2025
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IF 4.3
Yilong Xu,Shihao Fu,Yuxin Wen,Wenqing Lv,Liangyan Ma,Haoquan Hu,Lijun Jin
Limonene is significant feedstock and how to abundantly acquire or convert into high-value chemicals is crucial. Waste tire pyrolysis oil (TPO) rich in limonene provides an alternative route. To address its difficult separation from other compounds in TPO, especially p -cymene, a new route to convert limonene into α-terpinyl methyl ether (TME) with high boiling point was proposed by alkoxylation over heteropolyacid-modified Hβ zeolite. Strong Brønsted acid centers can attain high efficiency in alkoxylation in limonene- p -cymene binary compounds. 89.24 % conversion was achieved after 5 h at 60 °C while TME yield is the highest (71.70 %) after 3 h with no reaction of p -cymene. Above 80 % limonene is transformed in real fraction of 160–180 °C of TPO and TME yield exceeds 60 %. Initial separation of TME and aromatic was achieved by vacuum distillation. The possible mechanism of limonene alkoxylation was proposed. This work provides a new approach to efficient utilization of limonene.
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高影响因子
MACROMOLECULAR RESEARCH
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2025
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IF 3.4
Lv Changshun,Wang Qingshuang,Li Zhaoxin,Jiang Xue,Liu Tianhui,Xu Xiaoru,Feng Xiangru
Chitosan-based pluronic thermo-sensitive hydrogels represent a class of biomaterials characterized by their favorable biocompatibility and exceptional thermo-sensitive properties. The purpose of this study was to investigate their potential as carriers for embedding α-Mangostin (α-M), a bioactive compound derived from mangosteen peel, with the aim of inhibiting methicillin-resistant Staphylococcus aureus (MRSA). During the synthesis process, chitosan (CS), tannic acid (TA), pluronic F-127 (F127), and pluronic F-68 (F68) were combined to form the thermo-sensitive hydrogel, into which α-M was physically incorporated through crosslinking (designated as CTFF@α-M). We are very interested in the results obtained during the research process. The results of fluorescence imaging in small animals showed that CTFF extended the residence time of α-M in the body from 3 to 9 days, improved the bioavailability of α-M, and achieved the goal of sustained drug release. In vitro antibacterial experiments showed that CTFF@α-M (30 mg/mL) had an inhibition rate of 79.69% against MRSA, which was higher than CTFF@amoxicillin (AM) (5 mg/mL) increased by 71.68%. This study is expected to provide new insights into the integration of biomaterials and natural therapy, and emphasize the therapeutic potential of thermosensitive hydrogels and α-M in antibacterial treatment, especially in the fight against MRSA infection. Graphic In this study, a CTPP hydrogel was prepared using a physical cross-linking method, and α-M was encapsulated to create the CTPP@α-M drug-loaded hydrogel. Various characterization techniques, including SEM, FT-IR, and XRD, were employed to confirm its physicochemical properties. The hydrogel exhibited excellent water solubility, swelling capacity, sustained release characteristics, and biocompatibility, with the ability to transform into a semi-solid gel at 37 °C. In vitro experiments demonstrated that the CTPP@α-M hydrogel showed a powerful inhibitory effect on MRSA, exhibiting strong resistance to antibiotic resistance. Infection experiments using a mouse model confirmed that CTPP@α-M not only accelerated wound healing but also significantly reduced bacterial counts at the wound site. Moreover, the CTPP@α-M group significantly decreased the expression of pro-inflammatory cytokines while increasing the levels of anti-inflammatory factors, indicating its promising anti-inflammatory properties. Collectively, these data suggest that CTPP@α-M is a highly effective antibacterial thermo-sensitive hydrogel with excellent performance.
Diagram of the preparation of protocol CTFF@α-M and the treatment process of bacterial infection wounds
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ENVIRONMENTAL RESEARCH
·
2025
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IF 7.7
Yinling He,Zhendong Sun,Ning Wu,Chuanhai Li,Qunfang Zhou,Ligang Hu,Chunyang Liao,Guibin Jiang
Mounting evidence suggests that certain environmental contaminants can influence adipogenesis and function as obesogens, many of which act by activating the key regulator peroxisome proliferator-activated receptor γ (PPARγ). Synthetic phenolic antioxidants (SPAs) are ubiquitously detected in both environmental matrices and human samples, whereas their potential effects on lipid metabolism and the underlying molecular mechanisms remain largely unexplored. In this study, we aimed to assess the agonistic effects of SPAs on PPARγ, glucocorticoid receptor (GR), and retinoid X receptor α (RXRα), as well as their adipogenic potential. Luciferase reporter gene assays showed that among the ten commonly used SPAs evaluated, only 2,4,6-tri- tert -butylphenol (AO 246) exhibited efficacious agonistic activity toward RXRα. Environmentally relevant level exposure to AO 246 significantly promoted adipogenesis in 3T3-L1 cells, as evidenced by increased lipid accumulation and adipogenic gene expression, which could be alleviated by the RXRα antagonist UVI 3003. Further analysis revealed that the early stage of differentiation (Days 0–2) was the critical window for AO 246 to enhance adipogenesis and modify the gene expression profile. This study first demonstrated the adipogenic effects of AO 246 by activating RXRα but not PPARγ or GR, emphasizing its possible implications in obesity and associated health risks.
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PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2025
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IF 8.2
Ying-ying Gu,Ming-xuan Liu,Xin-ru Zhao,Ying Yi,Jia-yi Wang,Yi-lin Lin,Xiao-le Xu,Xiao-ling Zhang
Olfactory dysfunction is one of the earliest non-motor symptoms of Parkinson's disease (PD), accompanied by abnormal α-synuclein aggregation in the olfactory bulb (OB). We previously reported that Cordycepin (Cor), a potential anti-inflammatory and anti-oxidative adenosine derivative, has emerged as an attractive candidate for PD treatment. However, existing investigations have predominantly focused on the amelioration of motor symptoms rather than modulating the disease course from prodromal stage. This study aimed to evaluate if Cor exerts a disease-modifying effect at the prodromal stage prior to the onset of motor deficits in eight-week-old male C57BL/6 mice exposed to rotenone. Our results showed that Cor administration significantly attenuated olfactory dysfunction and delayed the onset of motor impairments. This therapeutic effect was concomitant with pathological α-synuclein aggregates degradation in OB. RNA sequencing analyses revealed that Autophagy-lysosomal pathway (ALP) in OB may be associated with the neuroprotective actions of Cor in PD. Furthermore, Cor significantly stabilized Calcyclin-binding protein/Siah1-interacting protein (CacyBP/SIP) via inhibiting its ubiquitin-proteasome degradation in rotenone-treated SH-SY5Y cells. This stabilization suppressed NLRP3 inflammasome activation and subsequently enhanced autophagosome-lysosome fusion, culminating in the autophagic degradation of α-synuclein. Notably, blockade of CacyBP/SIP abrogated the neuroprotective effects of Cor against rotenone-induced olfactory impairment. Collectively, these findings suggest CacyBP/SIP-NLRP3-α-synuclein axis may serve as a key target of Cor in the early treatment of PD, which provides novel insights into the mechanisms for the anti-PD effect of Cor.
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PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
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IF 5.6
Shaobing Zhang,Zhi Lian,Xingtao Chen,Ke Duan,Cheng Liang,Xin Duan,Jiyuan Yan,Jianhua Ge
Objective The effective treatment of bone tissue defects is a significant challenge in clinical orthopaedics. This study investigated the preparation and properties of composite bone cements comprising 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC)-modified α-calcium sulfate hemihydrate (α-CSH)/β-tricalcium phosphate (β-TCP) for potential applications in bone regeneration. Methods α-CSH was synthesised via a hydrothermal method, while β-TCP was prepared through a chemical reaction involving calcium salts and phosphates. HACC was dissolved in deionised water to formulate curing solutions with concentrations ranging from 0 to 4 wt% HACC. The α-CSH and β-TCP powders (7:3 mass ratio) were homogeneously blended with the HACC solution (1:0.3 w/v) to produce HACC/α-CSH/β-TCP composite bone cements. To assess the biocompatibility, osteogenic potential, and antimicrobial activity, extracts of the composite bone cements were used to culture rat bone marrow mesenchymal stem cells. A critical bone defect model in the femoral condyle of male Sprague–Dawley rats was employed to evaluate the in vivo osteogenic repair efficacy. Results The self-curing time met clinical requirements, and the compressive strength approached that of normal human cancellous bone. The in vitro degradation rate was consistent with the rate of new bone formation. Increasing the HACC concentration enhanced the injectability and washout resistance of the composites. The materials exhibited good in vitro biocompatibility; composites containing HACC upregulated osteogenesis-related gene expression and significantly inhibited the growth of Staphylococcus aureus and Escherichia coli . In vivo, the 3 wt% HACC/α-CSH/β-TCP composite showed the best overall performance. Conclusions HACC/α-CSH/β-TCP composite bone cements demonstrate promising clinical potential owing to their improved setting time, mechanical properties, injectability, and cytocompatibility. This material is a strong candidate as a substitute for artificial bone to treat infectious bone defects.
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PubMed
高影响因子
LANGMUIR
·
2025
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IF 3.9
Peng Wang,Qianpeng Dong,Chenyu Gao,Lianlian Wang,Ruiliang Wang,Dianming Chu,Wenjuan Bai,Yan He
This research presents a regulation strategy of constructing a heterogeneous bilayer catalyst of Fe and Al2O3, namely, Fe–Al2O3, through interfacial interaction and particle nucleation, in order to modulate the size and catalytic activity of Fe sites and achieve the growth of carbon nanotubes (CNTs). Through temperature control, the noncatalytically active Fe2O3 clusters on the surface of Al2O3 are exposed, inducing multiple nucleation of Fe clusters on its surface. Under the synergistic effect of the confinement at the Fe–Al2O3 interface, the fabrication of small Fe nanoparticles is accomplished. The results indicate that the catalytically active Fe nanoparticles have a diameter of approximately 10 nm, and the grown CNTs have a diameter of approximately 15 nm. Based on the systematic characterization results, the nonactive Fe2O3 clusters confined by strong interfacial interaction induce multiple nucleation of Fe on their surface during multiple loading processes, facilitating the formation of nanoparticles. Additionally, the strong interaction between Al2O3 and Fe induces the formation of FeAl4O8, thereby enhancing the thermal stability of the nanoparticles. In conclusion, the targeted regulation of the interfacial interaction of the catalyst active sites offers guidance for the low-cost and large-scale preparation of small nanoparticle catalyst particles.
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PubMed
高影响因子
Molecular Catalysis
·
2024
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IF 3.9
Fukun Li,Ronghe Yang,Jinhang Dai,Hanyue Deng,Junjun Shi,Wucheng Xie,Juanhua Kong,Tingwang Guo
Biomass upgrading, which supplies important biomaterials, chemicals and fuels, is considered as an efficient way to realize the goal of carbon neutralization. Recently, γ-valerolactone (GVL) has gained increasing attention due to its wide applications. In this work, a novel protocol of microwave-assisted cascade catalytic upgrading of α-angelicalactone (α-AL) to GVL over metal triflate using alcohol as the hydrogen donor was proposed. The yield of GVL production from α-AL upgrading is strongly influenced by the Lewis acid strength of the metal triflate and the reduction potential of the employed alcohol. Among all tests, Sc (OTf) 3 showed the best catalytic performance in isopropanol media, giving 96.4 % GVL yield and 100 % α-AL conversion at 433 K within 3.0 h. Meanwhile, kinetic study indicated transfer hydrogenation of isopropyl levulinate to isopropyl 4-hydroxyvalerate was the rate-limited step with the apparent activation energy of 94.5 kJ/mol. This Sc (OTf) 3 catalyst was highly stable and could be reused for five cycles by simple vacuum distillation. In a word, this work provides an alternative for GVL production from biomass upgrading.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2022
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IF 4.446
Lankai Su,Kun Chen,Shu Bai,Linling Yu,Yan Sun
Environmental-friendly treatment of poly (ethylene terephthalate) (PET) is important for the circular plastic economy, and enzymatic degradation provided a promising approach. However, enzyme binding toward the PET surface is a rate-limiting factor in PET hydrolysis . Thus, a 16-residue peptide (αSP) from the C-terminal sequence of α-synuclein was used to fuse to the C-terminal of a variant (ICCG) of the leaf-branch compost cutinase , and supposed to serve as an anchor peptide for PET degradation. The fusion enzyme (ICCG-αSP) was characterized to keep the same secondary and tertiary structures as ICCG, but enhanced enzyme binding onto bis(2-hydroxyethyl) terephthalate and PET, respectively. Moreover, αSP-fusion enhanced enzyme expression by over 50%, and increased the catalytic efficiency of p -nitrophenyl butyrate by 20% without compromising the enzyme stability . For PET degradation, ICCG-αSP was 3.89 times more active than ICCG by 3-h reaction at 50 °C, and exhibited 1.05–1.45 times higher degradation activity than ICCG in 4-d reaction at 50–70 °C, and pH 7.0 to pH 9.0, respectively. Furthermore, αSP-fusion lowered the optimal long-term degradation temperature from 65 °C to 50 °C at pH 8.0. Therefore, αSP-fusion was effective to increase the hydrolytic efficiency of PET hydrolases by increasing the degradation efficiency and decreasing the degradation temperature.
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高影响因子
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