BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2021
·Vol. 44
·期 10
·2193-2204
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IF 3.21
He Song,Wu Xiaomei,Ma Baodi,Xu Yi
The novel Ni-NTA-functionalized magnetic chitosan microspheres (MCS-NTA-Ni) were prepared via amino functionalization of MCS with epichlorohydrin and ethylenediamine, followed by the introduction of the aldehyde groups and NTA in turn, and nickel (II) ions were chelated in the end. MCS-NTA-Ni contained numerous long-armed NTA-Ni surface groups, ensuring high enzyme loading and providing more space and flexibility to attach enzymes and maintain their activity. This microsphere can have highly selective adsorption of his-tagged recombinant protein. The his-tagged recombinant Microbacterium esterase of E. coli BL21 (DE3)/pET21a-EstSIT01 was first immobilized on MCS-NTA-Ni by affinity fixation, giving high immobilization yield (90.1%) and enzyme loading (120 mg/g). Compared with free esterase, the immobilized esterase was found to exhibit higher pH stability and thermal stability. In addition, the immobilized esterase had excellent reusability for the synthesis of key chiral intermediate of d -biotin and the substrate conversion could still keep 100% after 8 cycles continuously.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2021
·
IF 4.432
Fujing Wang,Rong Hu,Jiaxing Zhang,Tingting Pei,Zhuo'en He,Liliang Ju,Zhongxiao Han,Mingqing Wang,Wei Xiao
Renal fibrosis is the pathological consequence of progressive chronic kidney disease . Although it has been reported that vitamin D3 exerts antifibrotic effects, the underlying mechanisms remain unclear. This study is aimed at investigating the effects and molecular mechanisms in high-dose vitamin D3 treatment on renal fibrosis. A model of chronic kidney disease was established by 5/6 nephrectomy in rats characterised by high levels of serum creatine, urea nitrogen, and urinary protein. Serum 25-dihydroxyvitamin D3, calcium and parathormone levels were measured to evaluate vitamin D levels. Hematoxylin and eosin , periodic acid Schiff and Mallory's Trichrome staining were used to evaluate histopathological changes in rats. Moreover, the expression of vimentin , collagen I, α-smooth muscle actin and E-cadherin were analyzed at molecular and histopathological levels. Our results showed that exposure to vitamin D3 decreased the levels of serum creatine, urea nitrogen and urine protein and restored the homeostasis of calcium and parathormone. Vitamin D3 also downregulated the expression of vimentin, collagen I and α-smooth muscle actin and attenuated renal fibrosis and epithelial to mesenchymal transition in the kidney. Importantly, vitamin D3 treatment increased the expression of the vitamin D receptor and inhibited Transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway in rats kidneys with chronic kidney disease. Mechanistically, the upregulation of TGF-β1 and phosphorylation of Smad3 induced by vitamin D3 was reversed by activation of the vitamin D receptor. Our findings indicated that vitamin D3 is a potential antifibrotic drug in chronic kidney disease via the vitmin D receptor and inhibiting TGF-β1/Smad3 signaling pathway.
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ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Jiangyi Mao,Xiaoyu Zhang,Shih-Hung Liu,Zhongjin Shen,Xing Li,Wenjun Wu,Pi-Tai Chou,Jianli Hua
The electron-accepting and anchoring group plays a significant role on the optical and electrochemical properties of an organic dye. They also affect the intramolecular charge transfer, the electron injection processes and the adsorption mode, hence the photostability of the dye on TiO 2 films. In this study, we have designed and synthesized two new D-A-π-A dyes ( RD-III and RD-IV ) with 2-(1,1-dicyanomethylene) rhodanine (DCRD) as electron-accepting and anchoring group. For comparison, an analogue of RD-III , namely CA-III , with cyanoacrylic acid (CA) as the acceptor was also prepared. We have carefully examined their optical and electrochemical properties, device performance and electrochemical impedance spectroscopy (EIS). Supplementary support is given by computational approach to gain in-depth insight into the adsorption states and electron contributions. The theoretical calculation of dye/(TiO 2 ) 38 displayed that the angle between the molecule of RD-III and the surface of TiO 2 was only 31.84 0 in contrast to 97.16 0 for CA-III . This adsorption state can facilitate dye aggregation and charge recombination, resulting in a decrease of short circuit current density ( J sc ) and open circuit voltage ( V oc ). Further improvement has been successfully made by adding long alkoxy chains with large steric hindrance. After introducing the alkoxy chains, the dihedral angle between RD-IV and TiO 2 increased to 42.61 0 and the steric hindrance can inhibit dye aggregation and charge recombination. Therefore, higher photoelectric conversion efficiency of 5.53 % was obtained with RD-IV in DSSC devices compared with 4.51 % for DSSC based on RD-III .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Huizhu Wang,Yan Li,Zhihui Ren,Zhongcheng Cong,Mengjie Chen,Lin Shi,Xu Han,Jin Pei
An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology. The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield. Specific conditions (microwave power, 575 W; microwave time, 18 min; liquid-to-material ratio, 13.5:1 mL/g; and enzyme dose, 6.5 g/mL) generated an experimental yield of 36.21 ± 0.62%, which closely agreed with the predicted value of 35.75%. Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2 × 10 3 to 7.4 KDa) and PR-2 (from 559.8 to 106.6 KDa). Subsequently, the antioxidant activity and α- d -glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α- d -glucosidase inhibitory activity than PR-2. Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method. The result showed that PR-1 contained mannose , ribose , rhamnose , glucosamine hydrochloride, glucuronic acid , galacturonic acid, glucose, galactose , arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.
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Toxics
·
2024
·Vol. 12
·期 1
·83
·
IF 3.9
Kunyu Zhang,Yunyi Yin,Man Lv,Xin Zhang,Meichen Zhang,Jia Cui,Ziqiao Guan,Xiaona Liu,Yang Liu,Yanhui Gao,Yanmei Yang
Limited studies have demonstrated that inorganic arsenic exposure is positively associated with serum vitamin D levels, although the correlation between urinary arsenic species and serum vitamin D has not been investigated in areas of water-borne arsenicosis. A cross-sectional study of 762 participants was conducted in Wenshui Country, Shanxi Province, a water-borne arsenicosis area. The results showed a positive relationship between urinary arsenic species (inorganic arsenic (iAs), methylarsonic acid (MMAV), dimethylarsinic acid (DMAV) and serum 25(OH)D. Log-binomial regression analysis indicated a 0.4% increase in the risk of vitamin D excess for every 1-unit increment in the Box–Cox transformed urinary DMAVafter adjustment for covariates. After stratifying populations by inorganic arsenic methylation metabolic capacity, serum 25(OH)D levels in the populations with iAs% above the median and primary methylation index (PMI) below the median increased by 0.064 ng/mL (95% CI: 0.032 to 0.096) for every one-unit increase in the Box–Cox transformed total arsenic (tAs) levels. Serum 25(OH)D levels increased by 0.592 ng/mL (95% CI: 0.041 to 1.143) for every one-unit rise in the Box–Cox transformed iAs levels in people with skin hyperkeratosis. Overall, our findings support a positive relationship between urinary arsenic species and serum 25(OH)D. It was recommended that those residing in regions with water-borne arsenicosis should take moderate vitamin D supplements to avoid vitamin D poisoning.
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ANALYST
·
2012
·Vol. 137
·期 18
·4235-4240
·
IF 4.23
Xiaoyu Mu,Li Qi,Ying Shen,Haizhi Zhang,Juan Qiao,Huimin Ma
A novel amino acid ionic liquid (AAIL) with L-ornithine (L-Orn) as anion was successfully synthesized, and subsequently applied as an available chiral ligand coordinated with Zn(II) in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for the enantioseparation of dansyl amino acids (Dns-D,L-AAs). The influence of key parameters, such as buffer pH, concentration ratio of Zn(II) to ligand and complex concentration, was investigated in detail. Eleven pairs of Dns-D,L-AAs enantiomers were baseline separated and three pairs were partly separated under the optimum conditions. For exploring its potential application, the quantitative features of this proposed method were studied. Good linearity (r2 = 0.999) and favorable repeatability (RSD ≤ 3.4%) were obtained by using Dns-D,L-Met as the test analyte. Finally, this method was employed to investigate the inhibition efficiency of D-amino acid oxidase (DAAO) inhibitors, which may pave a new way for the high-throughput screening of enzyme inhibitors and relevant drug discovery.
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JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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2018
·
IF 0.827
Liwei Cao,Tian Wei,Yihui Shi,Xiaofang Tan,Jianxin Meng
Two rapid and sensitive analytical methods are developed for the determination of D-penicillamine (D-PEN) and tiopronin (TP) through high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). A boron-dipyrrolemethene (BODIPY) fluorescence labeling reagent, 1,3,5,7-tetramethyl-8-bromomethyl-difluoroboradiaza-s-indacene (TMMB-Br) was successfully applied to derivatize these two thiol drugs. Fluorescein was used as the internal standard (IS) for the quantification of D-PEN and TP in CE-LIF. The derivatization and separation conditions were optimized carefully. Under the optimum conditions, the HPLC and CE separation of D-PEN and TP could be achieved within 12 min. The limits of detection were as low as 2.0 nmol/L for HPLC-FLD and 0.47 nmol/L for CE-LIF. The drugs in human urine and serum samples were determined successfully, and the recoveries were 95.0–06.7% and 95.2–104.3%, respectively.
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TETRAHEDRON-ASYMMETRY
·
2014
·
IF 2.165
Rui-Jie Chen,Gao-Wei Zheng,Yan Ni,Bu-Bing Zeng,Jian-He Xu
A new NADH-dependent carbonyl reductase Rh CR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl ( S )-8-chloro-6-hydroxyoctanoate [( S )-ECHO], an important chiral precursor for the synthesis of ( R )-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing Rh CR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L −1 (2 M) of ECOO were stoichiometrically converted to ( S )-ECHO in a space–time yield of 1580 g L −1 d −1 without the external addition of any expensive cofactor.
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TALANTA
·
2017
·
IF 4.162
Quanqing Zhang,Yuanyu Huang,Haofan Sun,Yangyang Gan,Huali Shen,Bin Jia,Pengyuan Yang
Novel 3-D floor-like ordered mesoporous carbon functionalized graphene composites, FLOMC-GO, with high graphitized carbon contents were successfully synthesized using a soft template method. The one-pot sol-gel method was employed to prepare the silica soft template. Then, the sandwich-like composites were further combined together to form a 3-D structure through pre-carbonization and carbonization . During these procedures, the sulphonyl and sulfide bridges were formed by cross-linking processes to connect the phenyl rings. The prepared FLOMC-GO was confirmed to have a large pore volume (1.03 cm 3 g −1 ), high BET surface area (544.99 m 2 g −1 ) and well-ordered mesoporous structure with a narrow pore-size concentrated at 3.74 nm. The content of carbon reached 80% and was highly graphitized. By taking advantage of the interactions between carbon and glycans, FLOMC-GO was utilized to enrich N-linked glycans from OVA and human serum. As expected, excellent size-exclusion was found during the enrichment of N-glycans released from OVA, and 25 N-linked glycans were identified. The intensity of glycans enriched by FLOMC-GO was 7 times to the result of active carbon, while the ratio of OVA digestion to BSA interfering proteins increased to 1:50. Additionally, 31 N-linked glycans in total were enriched from human serum. The relatively easy synthesis as well as ability to enrich N-linked glycans with high selectivity and efficiency makes FLOMC-GO a promising adsorbent material for the discovery of human serum biomarkers for disease diagnosis.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yan Liang,Li Xue,Feifei Xiang,Xiaojian Zhao,Lingmin Liang,Fengyi Liang,Yuhao Zhan,Ziteng Guo,Fangjun Liu,Yong Yang
A multi-interfacial NiO@In 2 O 3 /ZnO porous heterojunction array was designed. • The heterojunction array enabled highly efficient room temperature ammonia detection. • Multi-phase interfaces greatly enhanced the ammonia adsorption and charge transfer. • Porous array design and light activation further promoted the sensing reaction. • The work clarified the important role of multi-phase interfaces in gas sensing. Heterojunction engineering provides an effective means to enhance the gas sensing performance of metal oxide semiconductors through band structure optimization and interface effects. However, current research on heterojunction engineering primarily centers on binary heterojunctions (including p-n, n-n, and p-p junctions), while studies concerning ternary or multicomponent metal oxide heterojunctions for gas sensing remain exceedingly limited and challenging. Herein, a ternary NiO@In 2 O 3 /ZnO porous heterojunction array is fabricated in-situ on commercial alumina sensing substrate via a facile liquid-phase chemical reaction combined with topotactic transformation. The heterojunction array enables highly efficient room temperature ammonia detection under visible light illumination, exhibiting a high response of 263% towards 100 ppm ammonia, a fast recovery of 21 s, and a low detection limit of 0.483 ppm. Moreover, it displays excellent selectivity, strong resistance to humidity interference, and stable long-term performance. First-principles calculations integrated with spectroscopic characterization suggest that the construction of ternary NiO@In 2 O 3 /ZnO heterojunction with multi-phase interfaces greatly enhances the ammonia surface adsorption and promotes the interfacial charge transfer efficiency. Interestingly, there exists a notable positive correlation between the d-band center position of sensing materials and their adsorption behavior as well as sensing performance. This work not only achieves a multi-dimensional enhancement in the room temperature ammonia sensing performance of metal oxide semiconductors through ternary heterojunction construction, porous array design and external light field activation, but also significantly enhances the gas sensing mechanism of ternary heterojunctions at atomic and electronic scale, offering new insights for the rational designing of high-performance resistive gas sensors via multi-phase interfaces.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Ziyi Shao,Kun Fan,Hua Ying,Yiting Chen,Jiayu Yan,Jiajun Zhang,Jiahao Zhang,Zhirui Niu,Yanzhong Zhen,Jingyu Ren
Crystal field splitting energy governed the doping-induced d-band shift. • Anti-bonding orbital activation mechanism for O 3 , H 2 O 2 and PS was proposed. • Molecular dipole moment was the dominant factor for pollutant degradation. Modulating the d-band center of transition metals by doping engineering is an efficient strategy to enhance their catalytic efficiency. However, there remains a lack of systematic theoretical explanation for how doping engineering modulates the d-band center and activates antibonding orbitals at the electronic structure scale. Herein, using α-Fe 2 O 3 (αFO) as a model transition metal, machine learning, density functional theory calculations and experimental characterization were integrated to elucidate the doping induced d-band center shifts. The results reveal that dopants modulated the crystal field splitting energy of transition metals, which in turn tailors the d-band center, thereby enhancing the catalyst's ability to activate the antibonding orbitals of reactive species. This mechanism rationalized the efficient degradation of metronidazole observed in the Cu-αFO/DBD/persulfate process. Furthermore, to elucidate the degradation mechanism at the molecular level, this study investigated the structure-activity relationship, specifically how the molecular structure of the pollutants and the catalytic system influence the degradation efficiency. The data revealed the correlation between molecular properties (e.g., dipole moment and energy of the lowest unoccupied molecular orbital) and degradation kinetics. The proposed d-band center modulation and antibonding orbital activation mechanism provides both a theoretical foundation and a practical pathway for the rational design of high performance transition metal catalysts. Download: Download high-res image (388KB) Download: Download full-size image
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jinrui Dong,Pin Li,Xiaoyuan Ji,Yong Kang,Xue Yuan,Jingchun Tang,Boxiong Shen,Huajiang Dong,Honghong Lyu
Photocatalytic oxidation technology holds promise for ideal advanced treatment of antibiotic wastewater. Single-atom catalysts (SACs) are a new hotspot in catalytic science, but the photochemical studies on the removal of antibiotics from water and biocompatibility after entering the environment are scarce. In this work, we prepared a single Mn atom immobilized on N-doped biochar (Mn@N-Biochar) by impregnation calcination method for enhancing photocatalytic degradation of sulfanilamide (SNM) in different types of various water systems. Compared with the original biochar, Mn@N-Biochar showed enhanced SNM degradation and TOC removal capacity. DFT calculation concluded that the electrons of d‐orbital (Mn) and p-orbital (N) altered the electronic structure of biochar and enhanced the photoelectric performance. It was shown that Mn@N-Biochar caused negligible systemic inflammation and tissue damage when given orally in mice, and also did not alter cell death and ROS production in human lung, kidney, and liver cells, as compared with biochar. We are convinced that Mn@N-Biochar could enhance the photocatalytic degradation of antibiotics while maintaining biocompatibility, which could be a promising strategy for wastewater treatment .
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