SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Xiangli Shao,Longjiao Zhu,Yangzi Zhang,Zaihui Du,Chunyan Sun,Wentao Xu
Molecular logic gates possess great potential for multi-parameter sensing, intelligent diagnostics, and molecular computation. One remaining challenge was to construct novel new logic gates based on smart nanomaterials and functional nucleic acids. In this study, two novel new logic devices were constructed utilizing ion-tuned DNA/copper nanoclusters (CuNCs), which were dual-responsive to K + and H + . By merely altering the arrangement of a specific sequence of the DNA stabilizer, two kinds of well-designed DNA probes permuted by G-tracts, C-tracts, and poly-T were designed as the building blocks of the logic device. Poly-T acted as the template for the formation of CuNCs. G-tracts and C-tracts enabled a K + /H + -responsive conformational switch into G-quadruplex (G4) and i-motif structures. Such a conformational conversion significantly modulated the fluorescence behaviors of CuNCs. This study discovered the unique interaction between the logic device and graphene oxide (GO). The logic devices were implemented for the analysis of K + and microRNA.
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CyTA-Journal of Food
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2020
·
IF 1.653
Yongzhen Wang,Kai Zhu,Deguo Wang
The objective of this study was to develop a reliable visual method for rapid detection of donkey components to protect consumers from commercial adulteration. Six specific loop-mediated isothermal amplification (LAMP) primers targeted at donkey mitochondrial cytochrome b gene were designed, the mitochondrial DNA extraction simplified, the LAMP reaction system optimized, the specificity verified with mitochondrial DNA of horse, pork, cow, sheep, chicken, duck, and rabbit as negative controls, the detection limit determined with gradient dilution of adulterated meat with donkey meat, and the visual LAMP method for detection of donkey-derived ingredient in common meat products established. The results showed that the modified mitochondrial DNA extraction method was simple and repeatable, and the visual LAMP method with 4-(2-pyridylazo)-resorcinol sodium salt as indicator can accurately and specifically detect the donkey meat in common meat products, 1% detection limit. The study provided a promising solution for facilitating the surveillance of the commercial adulteration in processed meat.
研究领域:
食品科学
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分子生物学
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Meijuan Du,Qian Chen,Xueqin Xu
A novel and label-free electrochemical aptasensor based on exonuclease III (Exo III) and G-quadruplex DNAzyme has been first designed to detect metronidazole (MNZ) sensitively and selectively in this paper. Capture probe (CP), helper probe (HP) and aptamer (Apt) hybridize to form double-stranded DNA (dsDNA) structure with a blunt 3′-terminus, resulting in the guanine (G)-rich CP being enclosed in the dsDNA, followed by specific digestion of the 3′-terminus by Exo III to expose the G-rich sequence. At this time, CP can bind with the added hemin to form G-quadruplex DNAzyme which catalyzes the redox reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 , generating a strong current signal. However, in the presence of MNZ, the binding of MNZ and aptamer breaks the original blunt 3′-terminus of the dsDNA structure, blocking the subsequent series of reactions, and only a weak current signal can be observed. Under the optimal conditions, the aptasensor showed excellent sensitivity and specificity for the detection of MNZ with a wide linear range from 10 pM to 500 nM and a detection limit as low as 3.0 pM (S/N = 3). The aptasensor constructed based on MNZ aptamer can effectively avoid the interference of other nitro compounds and antibiotics to a certain extent. Compared with other specific recognition elements, the aptasensor possesses the advantages of strong specificity, simple preparation and low cost. Moreover, the proposed aptasensor has been successfully applied to the detection of MNZ in tap water and honey samples.
研究领域:
生物化学
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分析化学
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ACS Applied Materials & Interfaces
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2018
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IF 8.097
Hengchang Ma,Yanfang Qin,Zengming Yang,Manyi Yang,Yucheng Ma,Pei Yin,Yuan Yang,Tao Wang,Ziqiang Lei,Xiaoqiang Yao
Fluorescence-tunable materials are becoming increasingly attractive because of their potential applications in optics, electronics, and biomedical technology. Herein, a multicolor molecular pixel system is realized using a simple copolymerization method. Bleeding of two complementary colors from blue and yellow fluorescence segments reproduced serious multicolor fluorescence materials. Interestingly, the emission colors of the polymers can be fine-tuned in the solid state, solution phase, and in hydrogel state. More importantly, the positive fluorescent polymers exhibited cell-membrane permeable ability and were found to accumulate on the cell nucleus, exhibiting remarkable selectivity to give bright fluorescence. The DNA/RNA selectivity experiments in vitro and in vivo verified that [tris(4-(pyridin-4-yl)phenyl)amine]-[1,8-dibromooctane] has prominent selectivity to DNA over RNA inside cells.
研究领域:
生物医学工程
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材料科学
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分子生物学
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CHEMICO-BIOLOGICAL INTERACTIONS
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2026
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IF 5.2
Meng Yang,Yuping Xiao,Mingquan Huang,Shuang Liu,Guangrui Pan
Triclosan (TCS) is a broad-spectrum antimicrobial agent that has raised public health concerns due to its continuous environmental release and human exposure. Previous studies suggest that TCS may disrupt endocrine function. Nonetheless, the toxicological effects on the female reproductive system, notably on ovarian granulosa cells, and the underlying pathways remain poorly understood. This study was designed to examine the toxic effects of TCS on human granulosa cells and the related mechanisms. To this end, human granulosa cell lines (KGN and SVOG) were treated in vitro with varying doses of TCS. The results demonstrated that TCS exposure led to marked cytotoxicity and DNA damage in a dose-dependent manner. Transcriptomic profiling showed that TCS markedly affected pathways associated with mitochondrial respiratory chain function and DNA repair mechanisms within granulosa cells. Subsequent mechanistic studies revealed that TCS led to mitochondrial dysfunction, as evidenced by reduced activity of mitochondrial respiratory chain complex I and a marked increase in mitochondrial ROS levels. Application of the mitochondria-targeted antioxidant MitoTEMPOL significantly alleviated the ROS accumulation and DNA damage induced by TCS. Specifically, we found that TCS exposure suppressed the synthesis of glutathione (GSH), a key component of the endogenous antioxidant defense system. The decline in GSH synthesis was further confirmed to be an upstream initiating factor for TCS-induced mitochondrial ROS production and DNA damage in granulosa cells, as evidenced by glutathione monoethyl ester (GSH-MEE) pretreatment. These findings reveal a previously unrecognized molecular mechanism by which TCS impairs ovarian reserve function, thereby providing important theoretical insights for assessing the reproductive toxicity risk of TCS and suggesting that antioxidants targeting GSH and mitochondria may represent a potential intervention strategy.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2026
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IF 6.7
Yan Chen,Shuaiqi Feng,Zhifang Liu,Yanna Zhao,Chun Ji,Qingpeng Wang
Novel three-component platinum(IV) hybrids were developed as antitumor agents. • The conjugates were potent in inhibiting tumor proliferation and metastasis. • Activate by inhibiting fibrosis and DNA repair, and reversing multidrug resistance. • Potent in inhibiting inflammation and EMT, and boosting antitumor immunity. Fibrosis and DNA repair are crucial factors for cancer development and metastasis. Here, three-component platinum(IV) hybrids with captopril (CTP) and valproic acid (VPA) functional ligands were developed as antiproliferative and antimetastatic agents. These agents exerted effects by inducing DNA damage, suppressing fibrosis and inhibiting DNA repair. Upon activation, the platinum core bound to DNA and caused mitochondria-mediated apoptosis through the Bcl-2/Bax/caspase-3 pathway. The VPA moiety inhibited the key enzyme HDAC3, disrupted DNA-repair by modulating the proteins P-gp, PARP, and PTEN, and further overcame multidrug resistance (MDR). Furthermore, the TGF-β1/Smad2 and MMPs cascades were inhibited by CTP, while tumor inflammation was also inhibited through downregulating COX-2, TNF-α, and IL-6. These combined actions displayed a synergistic role in attenuating tumor fibrosis, and further reversed EMT. In addition, decreased PD-L1 expression and increased infiltration of CD3 + and CD8 + T cells were observed, leading to the initiation of antitumor immunity in tumors. Download: Download high-res image (308KB) Download: Download full-size image
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高影响因子
Biosensors-Basel
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2026
·Vol. 16
·期 5
·279
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IF 6.2
Lidan Niu,Siyu Huang,Jinmei Zhao,Wenjing Yang,Zhengnan Li,Siqi Niu,Jianchun Yang,Shiqi Chen,Qihui Wang
The safety of genetically modified crops, particularly the commercial cultivation of glyphosate-resistant genetically modified soybeans, has given rise to significant public concern. Consequently, there is an urgent need to develop efficient and precise methods for detecting genetically modified components. The present study constructed a novel electrochemical biosensor based on nucleic acid exonuclease III (Exo III)-assisted cyclic signal amplification and hairpin probe recognition for the highly sensitive and specific detection of the CP4-EPSPS gene in genetically modified soybeans. The sensor achieves exponential signal amplification by triggering Exo III to cyclically cleave the hairpin probe (H1) upon target DNA binding. Subsequent to this, the released DNA fragments hybridize with the methylene blue-labeled signal probe (HS-MB) that has been immobilized on the electrode surface. This process induces conformational changes and a decrease in the current signal, thereby enabling quantitative analysis of the target gene. The experimental phase of the study successfully validated the sensor’s mechanism and systematically optimized key parameters such as Exo III concentration and reaction time. In optimal conditions, the sensor demonstrated excellent linearity with target DNA concentrations ranging from 100 fM to 10 nM, achieving a detection limit as low as 0.1072 pM. Furthermore, it exhibited remarkable repeatability and stability. This study provides an analytical tool with broad application prospects for the rapid and precise detection of genetically modified crops.
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BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Feng Lin,Wanglong Li,Xinhuang Hou,Wei Lin,Xiaoling Lai,Xiaoqi Zheng,Luyao Li,Wenqian Sun,Jun Lin,Pinjie Lin,Feilin Lian,Fanggang Cai,Kun Wang,Yiquan Dai
Urinary extracellular vesicles (uEVs) provide noninvasive biomarkers for liquid biopsy owing to their ability to reflect disease-associated molecular alterations. However, the accurate analysis of low-abundance uEV surface proteins in complex biological matrices remains a major analytical challenge. Here, we report a proximity-activated dual-cascade (PADC) biosensing platform that integrates a target-responsive 3D DNA machine with a nanozyme-mediated ratiometric transduction circuit for high-fidelity multiplexed phenotyping of uEVs. Upon dual-recognition of target uEVs, the DNA machine undergoes proximity-induced spatial confinement on individual magnetic particles, creating a localized catalytic microenvironment that markedly enhances DNA walking kinetics and signal amplification efficiency. Coupled with an enzyme-nanozyme redox cascade, this confined catalytic process generates a robust ratiometric fluorescence output for sensitive and reliable molecular profiling. Benefiting from the spatially confined amplification architecture, the proposed platform enables ultrasensitive multiplexed analysis of CD63, EGFR, and PAK6 with detection limits down to 4.3 × 10 4 , 2.2 × 10 4 , and 1.7 × 10 3 particles/mL, respectively. Importantly, the target-uEV response was approximately six-fold higher than those generated by common urinary interferents or their mixture, demonstrating high analytical selectivity in complex urine matrices. To further evaluate its universality for multi-disease screening, multidimensional molecular profiling combined with machine learning algorithms was performed on clinical uEV samples collected from healthy controls, Diabetes Mellitus (DM) patients, and prostate cancer (PCa) patients. The optimal classification model achieved an overall multiclass diagnostic accuracy of 91% for distinguishing healthy, DM, and PCa groups. This work establishes a proximity-confined catalytic biosensing strategy for multiplexed uEV analysis and demonstrates its potential for uEV-based liquid biopsy and proof-of-concept disease stratification.
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Cell Host & Microbe
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2026
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IF 23.2
Weijie Wen,Runping Su,Yunyun Liu,Zhiyang Feng,Yancai Li,Jieli Li,Ziyu Huang,Fen Zhang,Shanshan Gao,Yingfei Ma,Qinghua Zhou,Emad M. El-Omar,Jeremy J. Barr,Tao Zuo
The human gut harbors a diverse virome, believed to play a crucial role in maintaining intestinal homeostasis. However, the magnitude of heterogeneity of the gut mucosal virome, and how it differentially regulates host inflammation phenotypes, such as those observed in Crohn’s disease (CD), remains unclear. Here, we identify two mucosal DNA virome enterotypes (E1 and E2) in humans, with E2 subjects exhibiting higher virome diversity and nuanced bacteriophage-bacteria correlations. Moreover, E2-enterotyped CD patients exhibited higher Wulfhauvirus abundance than healthy controls. The E2 virome is causally more proinflammatory in inducing intestinal inflammation in mice. Mechanistically, the novel E2-virome-enriched phage øBTZT001P (belonging to Wulfhauvirus ) lysogenically infects Bacteroides thetaiotaomicron , increasing sphingosine production. Downstream, sphingosine suppresses the commensal bacterium Blautia obeum in the gut, leading to worsened intestinal inflammation. Our findings reveal that configurational differences in the gut virome may dictate disease outcome through a phage-bacteria-metabolite-intestine axis, highlighting the importance of gut phage-bacteria crosstalk in health.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yuye Xuan,Yong Wei,Songquan Li,Chunlan Liu,Qian Yang,Zhihai Liu
To detect the degree of interaction between coptisine and different DNA G-quadruplexes (G4s) and screen its anti-cancer targets, this paper developed a highly sensitive fiber surface plasmon resonance (SPR) sensor using zirconium-based metal organic framework (MOF-808) nanomaterials and plastic coated multimode optical fiber, which solves the problems of background interference and low sensitivity in traditional fluorescence detection method. MOF-808 has the characteristic of high electron mobility, which increases the sensitivity of the sensor to 4869.03 nm/RIU and improves the performance by 107%. Meanwhile, thanks to the ultra-high specific surface area of MOF-808, coptisine is enriched on the sensor surface, providing more G4s binding sites, the detection limit for c-MYC Pu22 G4 is 11.15 nM. Three types of G4s were detected using the sensor, and the sensor showed a significant response to c-MYC Pu22 G4, indicating that coptisine has a strong targeting effect on Pu22 G4, which is consistent with the results of molecular docking simulation, confirming the effectiveness of the sensor as a drug target screening validation tool. This sensor exhibits good specificity, with no significant response to other substances such as double stranded DNA except for the substance being tested, and maintains excellent stability after long-term storage. This work provides a concept validation optical sensing platform for drug target screening.
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EBioMedicine
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2026
·
IF 11.2
Lei Zhang,Xiaotong Han,Xinran Gao,Hangyu Chen,Chunliang Shang,Long Chen,Jian Lin,Hongyan Guo
Background Adenomyosis diagnosis and classification relies primarily on magnetic resonance imaging or pathological examination. Liquid biopsy has been widely applied in oncology but remains underexplored for adenomyosis. We aimed to identify liquid-biopsy-derived 5hmC biomarkers to aid adenomyosis diagnosis and molecular subtyping. Methods Genome-wide 5hmC profiles were obtained by 5hmC-Seal from plasma cfDNA (adenomyosis: n = 51; controls: n = 46) and tissue DNA (adenomyosis: n = 26; controls: n = 21). A paired-tissue-driven splitting strategy was used: the training set comprised 26 adenomyosis plasmas with matched tissues plus 23 controls, and the validation set comprised remaining samples. A 5hmC-based logistic regression model was developed and validated. The identified marker amyloid precursor protein ( APP ) was functionally characterised in cellular and mouse models. Findings Ten 5hmC markers were identified by machine learning. The model showed a sensitivity of 0.88 and a specificity of 0.87 (AUC = 0.91) in the validation set, with better performance than CA125 and CA199. 5hmC signatures could distinguish intrinsic from extrinsic adenomyosis subtypes. APP was related to epithelial cell migration and fibrosis in experimental models, which indicated its potential involvement in adenomyosis pathophysiology. Interpretation Plasma cfDNA 5hmC markers show promise for adenomyosis diagnosis and molecular classification. The APP-HOXD9 axis represents a potential therapeutic target warranting further validation. These findings require prospective, multi-centre confirmation before clinical implementation. Funding This work was supported by grant 82274034 from the National Natural Science Foundation of China, grant 2022YFB3604700 from the National Key Research and Development Program of China, and project BYESS2022031 from the Beijing Association for Science and Technology–Young Elite Scientist Sponsorship Program (BAST-YESS).
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Huibin Zhang,Haiyang Yu,Juyue Dong,Zhen Gu,Lulu Li,Kaiyutai Zhou,Wei Jiang,Wei Tang,Huifeng Wang
Triblock polyA probe enables amplification-free, label-free SGGT sensing; • Interfacial engineering optimizes probe configuration, buffer, polyA length; • LoD for synthetic L. monocytogenes DNA reaches 2.1 × 10 −17 M; • Contaminated milk samples are detected with LoD of 10 3 CFU/mL; • The sensor shows good selectivity against other foodborne pathogens. The strong signal amplification capability of solution-gated graphene transistors (SGGT) provides a promising basis for highly sensitive biosensing. However, further improvement in sensitivity is often hindered by the molecular recognition interface on the functionalized sensing electrode. In this study, an amplification-free and label-free SGGT DNA biosensor was developed by using a triblock polyadenine (polyA) DNA probe. The sensor interface was systematically optimized by capture-probe configuration, buffer composition and polyA length. Using Listeria monocytogenes ( L. monocytogenes ) as a model target, the sensor achieved limits of detection (LoD) of 2.1 × 10 −17 M for synthetic DNA fragments and 10 3 CFU/mL for L . monocytogenes in contaminated milk samples. These results demonstrate the high sensitivity and good target distinguishability in mixed samples of the sensor, addressing key limitations of conventional methods. The established sensor platform offers a new approach for achieving high-sensitivity DNA detection, with potential applications in pathogen monitoring, food safety, and molecular diagnostics. Download: Download high-res image (176KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）