INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Zhuohao Xie,Peng Li,Haonan Zhan,Liuyan Zhuang,Sijian Tao,Liehua Deng,Jiahao Huang
Efficient and accurate detection of melamine in dairy products remains a crucial yet challenging task. Herein, an allosterically modulated G-quadruplex-integrated aptamer is rationally designed with thymine-rich recognition termini for melamine binding. The detection process is facile by simply introducing the analyte into the mixture consisting of G-quadruplex aptamer probes, exonuclease III, and thioflavin T (ThT). The detection feasibility is confirmed by the polyacrylamide gel electrophoresis and fluorescence measurement results. This exonuclease III-assisted signal amplifiable approach works well in a linear range from 0.1 nM to 0.1 μM. Moreover, a detection limit as low as 83 pM is easily achieved, which is almost five orders of magnitude smaller than the maximum allowable melamine levels (about 8 μM) defined by many countries all over the world. The whole assay time for each test is no longer than 1 h. Additionally, the scheme is highly specific and satisfactory recovery rates (from 91% to 104%) are readily obtained when challenged with melamine-spiked milk samples. Therefore, the label-free, turn-on, low-cost, and time-efficient method can be used for reliable detection of melamine in an easily manipulated and ultra-sensitive manner, which may find its utilization in the field of food safety, biomedical engineering, and clinical diagnosis.
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Materials Today Bio
·
2026
·
IF 11
Chongqing Yu,Zujiang Liu,Jin Ye,Ruixin Zhang,Min Ren,Fulin Deng,Anning Zhao,Ali Abou-Hassan,Yichun Wang,Yao Zhang,Valentim A.R. Barão,Liqun Xu,Jie Xu,Xi Rao
Visible-light-driven antibacterial photocatalysis holds great promise for infected wound management; however, efficient and biologically compatible heterointerfaces remain limited. Here, we constructed a graphdiyne-enabled TiO 2 /g-C 3 N 4 based bio-heterojunction (TCN/GDY) by growing graphdiyne (GDY) onto a TiO 2 /g-C 3 N 4 scaffold, which couples enhanced visible-light harvesting with antibacterial wound therapy. Structural characterization, photoelectrochemical measurements, valence band analysis, in situ XPS, electron paramagnetic resonance, and density functional theory support interfacial electronic coupling, an enhanced photoresponse, and reactive oxygen species generation. These observations are consistent with a proposed dual S-scheme like mechanism. Under 420 nm LED irradiation, TCN/GDY achieved >99% elimination of Escherichia coli , 99.40 ± 0.34% elimination of Staphylococcus aureus , and 98.65 ± 0.74% elimination of methicillin-resistant S. aureus ( MRSA ), and reduced retained crystal-violet-stained MRSA biofilm biomass by 78.57 ± 2.38% under the tested treatment and washing conditions. Multi-omics analysis identified treatment associated changes in central metabolism and nutrient transport. In an MRSA -infected mouse wound model, visible-light-activated TCN/GDY reduced the bacterial burden by 97.98 ± 1.04% and decreased the residual wound area to 2.93 ± 1.46% by day 11. This study establishes a graphdiyne-enabled TiO 2 /g-C 3 N 4 -based bio-heterojunction as a visible-light antibacterial photocatalytic platform for infected wound healing.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2026
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IF 6.7
Jing Liu,Hongxi Zhu,Lin Liu,Luliang Liao,Lingfang Qiu,Xun Xu,Youliang Shen,Ping Li,Xiaoyue Shi,Shuwang Duo
Nitrogen-defect-rich DCN enables Mn–N interfacial coupling with MnCo 2 S 4 . • Interfacial Mn–N bonding and S-scheme charge transfer promote efficient charge separation. • Enhanced ROS generation is achieved in the heterojunction under visible-light irradiation. • Optimized catalyst shows high visible-light activity and stability for TC degradation. • Degradation pathways and toxicity evolution demonstrate the deep mineralization and detoxification of TC. Constructing well-defined heterointerfaces is crucial for regulating charge separation and surface reactions in photocatalysts. Herein, MnCo 2 S 4 /g‑C 3 N 5 S-scheme heterojunctions were fabricated via an in-situ growth strategy, in which defect-associated nitrogen sites in g‑C 3 N 5 act as coordination centers to anchor Mn, forming well-defined Mn–N interfacial bonds. XANES/EXAFS analyses and density functional theory calculations confirm the presence of Mn–N coordination at the interface and reveal its role in tailoring the local coordination environment and electronic configuration of Mn. The Mn–N coupled heterointerface promotes directional separation and transfer of photogenerated carriers, as evidenced by photoelectrochemical measurements, electron spin resonance and femtosecond transient absorption spectroscopy, which demonstrate accelerated charge migration and enhanced generation of reactive oxygen species. Benefiting from this interfacial Mn–N bonding-driven charge separation, the optimized MnCo 2 S 4 /g‑C 3 N 5 -II heterojunction achieves markedly improved visible-light photocatalytic activity toward tetracycline degradation, reaching ~90.8% removal within 120 min with an apparent rate constant of 0.01371 min −1 , which far surpasses the individual components. This work highlights interfacial Mn–N bonding engineering as an effective strategy for constructing robust S-scheme heterojunctions with promoted charge separation and surface redox processes. Download: Download high-res image (182KB) Download: Download full-size image
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Frontiers in Microbiology
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2021
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IF 5.64
Cui Fang,Luo Peng,Bai Yao,Meng Jiangping
Deaths due to invasive fungal disease (IFD) have been increasing every year. Early and rapid detection is important to reduce the mortality rate associated with IFD. In this study, we explored a novel diagnostic method for detecting IFD, which involves the G Factor α subunit (GFαSub) from Limulus polyphemus. The GFαSub double-sandwich method was developed to detect (1,3)-β-D-glucans in human serum using purified GFαSub and horseradish peroxidase-labeled GFαSub. The GFαSub double-sandwich method and the G test were performed and compared. Using GFαSub sequence analysis, the expression plasmid pET30a-GFαSub252-668 was synthesized, and GFαSub252-668 was expressed and purified via isopropyl-β-d-thiogalactoside induction and nickel-nitrilotriacetic acid affinity. The optimization method was established via the orthogonal method. Using this method, the sera of 36 patients with IFD and 92 volunteers without IFD underwent detection, and the receiver operating characteristic curve of the GFαSub252-668 double-sandwich method was described. The sensitivity and specificity of the GFαSub252-668 double-sandwich method were 91.67 and 82.61%, respectively, and there was good correlation with the G test for the serum specimens of 36 patients with pulmonary IFD (R2 = 0.7592). In conclusion, our study suggests that the GFαSub252-668 double-sandwich method was satisfactory at detecting IFD cases. This method can be promoted and further developed as a novel method for diagnosing IFD.
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ACS Applied Polymer Materials
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2021
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IF 4.089
Yu-Zhuang Gao,Jun-Cai Chen,Yi-Xian Wu
Amphiphilic quaternized alginate-g-polytetrahydrofuran (QA-g-PTHF) graft copolymers were successfully synthesized via nucleophilic substitution of living PTHF+ chains obtained via cationic ring-opening polymerization with the −OH side functional groups in QA. This unique graft copolymer exhibits low cytotoxicity and anti-protein adsorption. The QA-g-PTHF nanospheres (328 ± 21 nm) loaded with curcumin exhibit ninefold higher anticancer effect to HeLa cells than pure curcumin due to the synergistic roles from the graft copolymer. Both the interactions of cationic quaternary ammonium with the negatively charged HeLa cell surface and the hydrophobic PTHF branches with the hydrophobic cell membrane result in a great improvement in the entrance of nanospheres into HeLa cells and effective drug release therein. The QA-g-PTHF/Ag nanocomposites prepared in situ during the synthetic process demonstrate more than 98% killing efficiency for Escherichia coli and Staphylococcus aureus. To the best of our knowledge, this is the first example of an amphiphilic, anti-protein, and antibacterial QA-g-PTHF copolymer with low cytotoxicity for potential anticancer applications.
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FUEL
·
2022
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IF 8.035
Qi Zhang,Zhen Zhang,Wanli Xia,Yaming Zhou,Hongmin Yang
Lauric acid (LA) shows much potential as one organic phase change material (PCM) for thermal energy storage, owing to its suitable melting point, relatively high thermal/chemical stability and latent heat. However, the LA-based thermal storage method invariably suffers from the big challenges of liquid leakage tendency and low thermal conductivity of single lauric acid, limiting immensely its practicality. Herein, one encapsulation and modifying strategy to fabricate high conductive and low liquid leakage composite phase change materials (CPCMs) for photo-thermal storage was proposed by constructing novel graphene doped LA/g-C 3 N 4 CPCM. Firstly, LA is infiltrated into a novel two-dimensional porous matrix – the graphitic phase carbon nitride (g-C 3 N 4 ) to prevent liquid leakage in the solid–liquid phase change process. Then it is further incorporated with a certain amount of graphite nanoplatelets to further enhance thermal conductivity. The encapsulation and modifying strategy enable the graphene doped LA/g-C 3 N 4 to show excellent thermal storage performance for both heat and light energy, low liquid leakage, and high thermal stability. The hybrid LA/g-C 3 N 4 with 5 wt% graphene (CPCM3) modification has a melting temperature of 45.88 °C and enthalpy of 159.19 J/g. The corresponding solidification temperature and enthalpy are 43.36 °C and 144.40 J/g, respectively. Its thermal conductivity at 50 °C is enhanced to 0.9338 W/(m·K) (one time higher than single LA), also superior to the state-of-the-art LA-based CPCMs. After 100 thermal cycles, the composite phase change material only suffers low mass loss and latent heat loss of phase change, which proves the good thermal reliability. Furthermore, TGA and DTG data indicate that CPCM3 can maintain good thermal stability in the normal operating temperature range. In the whole, the joint effect of the porous g-C 3 N 4 matrix and graphene modification improve the thermal storage performance of LA through the improvement of liquid immobilization and thermal conductivity. This work provides a promising strategy for fabricating high conductive and low liquid-leakage CPCM toward enhanced photo-thermal storage.
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
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2022
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IF 3.407
Lianqing Yu,Xingyu Zhao,Jiandong He,Lijie Duan,Yankun Wang,Yaping Zhang,Haifeng Zhu
It is extremely desirable to develop efficient and cheap water splitting photocatalysts. An ultrahigh performance and stable photocatalyst g-C 3 N 4 /Zn 0.5 Cd 0.5 S was prepared by simple sintering method. g-C 3 N 4 nanosheets are evenly distributed around 20–30 nm ZnCdS nanoparticles, which can accelerate carriers separation and transfer, and increase the carriers life-time from 4.1 ns to 5.5 ns. UV–vis absorption spectra demonstrate that their absorption range can reach to visible light edge of 539 nm. The photocatalyst has a high photocatalytic hydrogen evolution rate of 16.66 mmol•h −1 •g −1 under visible light illumination (λ = 400–780 nm). Furthermore, after six recycles of photocatalyst, the hydrogen evolution stability still remains 94%. The excellent photocatalytic performance is attributed to the Z-scheme energy band structure formed between g-C 3 N 4 and Zn 0.5 Cd 0.5 S by simple sintering method. This work provides significant progress toward the synthesis of cheap Z-scheme photocatalysts for practical applications.
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International Journal of Nanomedicine
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2017
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IF 4.3
Guangtao Xu,Huan Gu,Bo Hu,Fei Tong,Daojun Liu,Xiaojun Yu,Yongxia Zheng,Jiang Gu
Brain ischemia/reperfusion (I/R) injury (BI/RI) is a leading cause of death and disability worldwide. However, the outcome of pharmacotherapy for BI/RI remains unsatisfactory. Innovative approaches for enhancing drug sensitivity and recovering neuronal activity in BI/RI treatment are urgently needed. The purpose of this study was to evaluate the protective effects of tumor necrosis factor (TNF)-α-loaded poly(ethylene glycol)-b-(poly(ethylenediamine L-glutamate)-g-poly(L-lysine)) (TNF-α/PEG-b-(PELG-g-PLL)) nanoparticles on BI/RI. The particle size of PEG-b-(PELG-g-PLL) and the loading and release rates of TNF-α were determined. The nanoparticle cytotoxicity was evaluated in vitro using rat cortical neurons. Sprague Dawley rats were preconditioned with free TNF-α or TNF-α/PEG-b-(PELG-g-PLL) polyplexes and then subjected to 2 hours ischemia and 22 hours reperfusion. Brain edema was assessed using the brain edema ratio, and the antioxidative activity was assessed by measuring the superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content in the brain tissue. We further estimated the inflammatory activity and apoptosis level by determining the levels of interleukin-4 (IL-4), IL-6, IL-8, IL-10, and nitric oxide (NO), as well as the expression of glial fibrillary acidic protein (GFAP), intercellular adhesion molecule-1 (ICAM-1), and cysteine aspartase-3 (caspase-3), in the brain tissue. We provide evidence that TNF-α preconditioning attenuated the oxidative stress injury, the inflammatory activity, and the apoptosis level in I/R-induced cerebral injury, while the application of block copolymer PEG-b-(PELG-g-PLL) as a potential TNF-α nanocarrier with sustained release significantly enhanced the bioavailability of TNF-α. We propose that the block copolymer PEG-b-(PELG-g-PLL) may function as a potent nanocarrier for augmenting BI/RI pharmacotherapy, with unprecedented clinical benefits. Further studies are needed to better clarify the underlying mechanisms.
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2020
·Vol. 20
·期 3
·1685-1696
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IF 1.134
Wang Weijing,Han Na,Yang Chao,Zhang Wenxin,Miao Jinlei,Li Wei,Zhang Xingxiang
The process in which the nanofiber membrane is used to remove heavy metal ions and separation of oil-water solution is analyzed. Herein, smooth structures are induced by rGO-g-PAO sheets, which could be attributed to the strong interaction between P(AN-MA) and rGO-g-PAO. It is rewarding
to note that the P(AN-MA)/rGO-g-PAO nanofiber membrane would exhibit superhydrophilic traits in the air and ultra-low oil-adhesive traits underwater when the concentration of P(AN-MA) and PAO is 13 wt.% and 0.3 wt.%, respectively. The amidoxime (–C(NH2) NOH) groups on the
membrane surface can efficiently adsorb copper (Cu(II)) (1.65 mmol/g) and chromium (Cr(VI)) (4.70 mmol/g) ions in the waste water. Meanwhile, the P(AN-MA)/rGO-g-PAO nanofiber membrane exhibits ultrahigh flux (~6150 LMH), satisfying rejection rate (~97%) and outstanding flux recovery ratio
(~99%) in separating oil water emulsion.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
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IF 7.129
Chun Yang,Xiao Liu,Jun Liu,Guangli Li,Lixia Xie,Xiaohui Shi,Yukun Zhao,Lan Long,Dongge Ma,Jianxin Tang,Wanhong Ma
Conventional photocatalysts generate numerous active species—primarily hydroxyl radicals (•OH)—under solar light excitation to exert photocatalytic activity for especially antibacterial effects. However, the light dependence limits their competitiveness against other antimicrobial materials since they do not work at night. Herein, a P-g-C 3 N 4 /Sr 2 MgSi 2 O 7 :Eu 2+ ,Dy 3+ (P-g-C 3 N 4 /SMSO) composite day–night photocatalyst is synthesized, using a model methyl orange (MO) substrate, and the impacts of trace P doping and the SMSO composite on the activity of the photocatalyst in MO degradation is investigated; Its antibacterial effect against Escherichia coli and Staphylococcus aureus on ceramic surfaces is further examined. The morphology, structure, and composition of the photocatalyst are characterized by SEM, TEM, XRD, FT-IR, and UV–vis DRS. Finally, the photocatalytic mechanism is elucidated through active species capture experiments and ESR testing. P doping and the SMSO heterojunction structure reduce the width of the forbidden band of g-C 3 N 4 and broaden its visible-light-response range. Moreover, SMSO acts as a light source to realize long-lasting photocatalytic performance of the composite, even in the dark. The photocatalytic process produces •O 2 - , 1 O 2 , and h + active species, with •O 2 - and 1 O 2 playing the dominant role—instead of •OH as previously thought.
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FUEL PROCESSING TECHNOLOGY
·
2022
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IF 8.129
Mingyu Cui,Chong Liang,Wei Zhao,Xutang Liu,Linyang Dong,Dingkai Wang,Shuiyuan Fu,Zhijie Jiang,Fei Wang,Xianyong Wei
As the most abundant resource composed of aromatic groups in nature, lignin harbors significant potential for the production of bio-based fuels and chemicals. In recent years, solar-driven photocatalysis with its mild, low cost and environmental benign, has been employed for lignin depolymerization . Herein, we designed a Z -scheme CdS/H 5 PMo 10 V 2 O 40 /g-C 3 N 4 (CdS/HPA-2/CN) photocatalyst via hydrothermal method for the photocatalytic depolymerization of lignin. The prepared CdS/HPA-2/CN presents a nanoflower-like morphology with the average diameter of ∼600 nm. With the presence of electron-mediated HPA-2, CdS/HPA-2/CN exhibits the enhanced separation and transfer capacity of photogenerated charge carriers through Z -scheme mechanism. Result shows that the total yield ( Y ) of vanillin and vanillic acid from lignin depolymerization over optimized CdS/HPA-2/CN can reach to 32.7 mg/g lignin in DMF/formic acid ( v / v = 40/10) for 4 h. Reusability experiment of optimized CdS/HPA-2/CN displays that Y (26.5 mg/g lignin) can still reach to 81.0% of initial one after three cycles. Qualitative analysis of depolymerized products reveals that most components are aromatic compounds with the dominant products of vanillin and vanillic acid. Evidence also shows that photocatalysis promotes the reaction and small molecular products increased significantly after depolymerization. In addition, mechanism experiments and electron spin resonance tests exhibit that h + , ·O 2 − and ·OH took part in lignin depolymerization together and the effect sequence was ·O 2 − > ·OH > h + . In this work, as an electron mediator, HPA-2 provides the inspiration for construction of Z -scheme heterojunction , and CdS/HPA-2/CN exhibits enormous potential in lignin depolymerization by photocatalysis.
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 15
·4817-4825
·
IF 6.177
Shilin Kang,Zhijun Li,Zhikun Xu,Ziqing Zhang,Jianhui Sun,Ji Bian,Linlu Bai,Yang Qu,Liqiang Jing
Although g-C3N4-based heterojunctions show promise for photocatalytic CO2 reduction, enhancing charge separation and creating sufficient catalytic sites remain challenges. In this study, mixed-valence CuPc/graphene/g-C3N4 (HT-CuPc/G/UCN) ultrathin heterojunctions (∼4.8 nm thickness) with Cu1+/Cu2+ species were constructed by H-bond induced assembly and subsequent post-treatment with H2. The optimized H100-CuPc/G/UCN sample showed 36-times higher activity than bare ultrathin g-C3N4 (UCN) for photocatalytic CO2 reduction to CO. Based on atomic force microscopy images, normalized photocurrent action spectra, electron paramagnetic resonances, and in situ diffuse reflectance infrared Fourier transform spectra, it was confirmed that the unexpected photocatalytic activity of H100-CuPc/G/UCN was attributed to the dual-functional roles of the introduced graphene: to increase the optimized amount of CuPc and enhance the high-level-energy electrons transferring from UCN to CuPc and the created Cu1+/Cu2+ species by H2 reduction as active catalytic sites for CO2 reduction. This work provides a comprehensively feasible strategy for preparing rational heterojunctions toward high-efficiency solar-driven CO2 reduction.
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危险品化学品经营许可证（带存储）