MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Miao Ma,Qing Wang,Qianqian Su,Weizhe Liu,Hui Xia,Caixia Zhou,Mengtian Li,Fang Yang,Fujian Xu
Blood uric acid (UA) levels are closely associated with various diseases. This study presents a novel UA photothermal detection system. UA reacts with uricase to generate hydrogen peroxide, which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of copper ions as the catalyst. Crucially, instead of expensive laser, Light Emitting Diode (LED) was applied to offer the irradiation on oxTMB, inducing a temperature increase. The increment of temperature measured by a handheld household thermometer exhibits a linear correlation with the concentration of UA in the samples. This method demonstrates enhanced detection sensitivity and excellent anti-interference capabilities. It has been successfully applied to precisely determine UA levels in volunteer blood samples. The sensing platform achieves portable UA detection in human blood within 3 min, featuring a linear response range of 50–400 μM, a detection limit (LOD) of 14.4 μM. This work establishes a novel miniaturized LED-based photothermal (LED-PT) uric acid detection device, holding significant potential for rapid, portable UA monitoring in healthcare and clinical diagnostics.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenjing Li,Mengqing Xu,Zhuoyun Xia,Zheng Wang,Yingying Feng,Hui Li,Chuang Jiang,Yingnan Liu
The highly selective and sensitive detection of paraoxon residues at low environmental concentrations remains challenging. Herein, a novel fluorescent metal-organic framework composite (Ce 4+ @UiO-66-NH 2 ) with excellent phosphatase-like activity was developed to catalyze the hydrolysis of paraoxon into yellow p -nitrophenol ( p -NP), which quenched the composite's blue fluorescence via the inner filter effect. Based on this mechanism, a simple, portable, non-biological colorimetric/fluorescent hydrogel nanosensor (CM-SA spheres) was constructed by incorporating red fluorescent Mn-CDs with aggregation-induced emission as an internal reference. Upon increasing paraoxon concentration, the spheres showed visible color changes from colorless to yellow under daylight and blue to red under UV light. The sensor demonstrated a visual detection sensitivity of 0.05 μg mL −1 and limits of detection of 0.013 μg mL −1 (daylight) and 0.027 μg mL −1 (UV). In real sample analyses of tea and water, the CM-SA spheres exhibited strong anti-interference capability and reliability, offering a practical strategy for paraoxon degradation and detection.
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PLoS Pathogens
·
2025
·Vol. 21
·期 12
·e1013774
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IF 4.9
Biying Zhang,Taoxun Zhou,Runxin Zhu,Peixi Qin,Juncheng Li,Chunqun Wang,Hui Liu,Min Hu
Hammerhead ribozymes have found extensive applications in gene expression regulation across diverse biological systems including Escherichia coli , yeast, plants, and mammalian cells. However, their implementation in parasitic nematodes remains unexplored. Strongyloides stercoralis emerges as a particularly valuable model organism for studying developmental transitions in parasitic nematodes due to its unique life cycle alternating between parasitic and free-living stages. To expand the experimental toolkit for investigating developmental, evolutionary, and behavioral processes in this species, we established a conditional gene regulation system through transgenic integration of synthetic ribozyme constructs and demonstrated efficacy in regulating both exogenous ( mrfp ) and endogenous ( unc-22 ) gene expression through targeted RNA processing mechanisms. Focusing on the insulin/IGF-1 signaling pathway, a critical regulator of parasitic nematode development and longevity, we implemented ribozyme-mediated post-transcriptional control to dissect functional divergence between two isoforms of the insulin receptor homolog Ss -DAF-2. Comparative analysis revealed isoform-specific characteristics: while both isoforms maintain conserved signaling functions, isoform B exhibits specific binding affinity for human insulin and demonstrates significant transcriptional upregulation during parasitic transition phases. This ligand selectivity profile suggests that isoform B may serve as a molecular interface for host-derived insulin signaling coordination during parasitism. This study established a programmable ribozyme tool in S. stercoralis , functionally discriminated the two Ss -DAF-2 isoforms through precision RNA engineering, and identified isoform-specific ligand preferences with implications for host-parasite signaling. Our findings not only validate ribozyme-based approaches for genetic manipulation in parasitic nematodes but also lay the groundwork for future implementation of synthetic RNA switches in helminth research.
Hammerhead ribozymes have been widely used as gene regulation tools in various biological systems, yet their potential in parasitic nematode research remains unexplored. In this study, we established the first synthetic ribozyme-based transgenic regulatory system in human and canine parasitic nematode, Strongyloides stercoralis . By targeting RNAs for degradation, we successfully regulated both exogenous (monomeric red fluorescent protein, mrfp ) and endogenous ( Ss-unc-22 ) gene expression. Focusing on the insulin/IGF-1 signaling pathway, which governs larval development and lifespan in nematodes, our analysis revealed functional differences between two isoforms of the insulin receptor homolog Ss -DAF-2. Notably, Ss -DAF-2B showed specific binding to human insulin and significant upregulation during developmental recovery following arrest. This ligand-selective feature highlights isoform B’s potential in orchestrating host-parasite interactions. Our work not only provides a new method for genetic manipulation in parasitic nematodes but also reveals the evolutionary strategy by which parasites hijack host insulin signaling to regulate their life cycles. The finding provides important clues for identifying novel anti-parasitic drug targets and marks a significant step forward in addressing antiparasitic drug resistance and advancing innovative therapeutic strategies against infections.
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ACS Nano
·
2025
·
IF 16
Wen Ou,Na Wen,Huitong Deng,Qianqing Jiang,Dianyi Liu
Artificial photoreceptor-based retinal prostheses are a therapeutic strategy for the treatment of retinal degenerative diseases. Currently, it is challenging to construct an artificial retinal prosthesis based on a biocompatible neural interface using simple methods to achieve efficient neuronal stimulation under low light intensity and simulate visual resolution. Here, we demonstrate an effective strategy to dramatically enhance the sensitivity and selectivity of neuronal stimulation by constructing an antibody-modified photovoltaic biointerface between artificial photoreceptor devices and neuronal cells. Neurons cultured on the antibody-modified photovoltaic biointerface can be successfully activated through light irradiation with a light intensity lower than 5 mW cm–2, which is dramatically lower than the required light intensity of 1-Sun conditions for traditional artificial retinal prostheses. Benefiting from the antibody-modified interface for specific targeting, an arrayed distribution of neurons was achieved on the biointerface with Au arrays. This study develops an artificial photoreceptor system for synergistic integration of guided cellular growth and enhanced neural photoactivation, proposing a potential method for retinal prostheses and visual restoration.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xingtao Zhou,Xiaoyu Zhang,Haoran Hong,Junfan Lin,Yixun Xia,Ping Liu,Qin Xiang
Kung Pao Chicken (KPC) is a famous Sichuan cuisine with unique flavor. However, there is lack of thorough research about its flavor, which seriously limits its industrial production. Therefore, the aroma characteristics and the key aroma compounds of five commercial KPCs were investigated by sensory analysis combined with electronic nose, GC×GC-MS, gas chromatography-olfactometry-mass spectrometry (GC-O-MS), odor activity value (OAV) and odor-specific magnitude estimation (OSME). The results revealed that the typical aromas of KPCs were sweet, sour, sauce fragrant, spice, chicken and roasted flavor, especially sample A4. The volatile compounds of KPCs obviously varied among different brands. Orthogonal partial least squares discrimination analysis (OPLS-DA) successfully discriminated sample A4 from others and recognized 33 significantly different compounds. Based on OAV and aroma intensity (AI), 45 aroma-active compounds with OAV > 1 and AI > 2.5 were perceived in the five KPCs, in which 22 key aroma compounds were verified in sample A4 by aroma recombination and omission tests. Further correlation analysis found W1C and W5C as critical E-nose sensors to monitor the formation of KPC key aroma compounds. These results could establish the aroma database of KPC and provide the basis for its flavor stability during industrial production.
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DRUG METABOLISM AND DISPOSITION
·
2025
·
IF 4
Yong Li,Ying Ding,Chenxu Liu,Yunchao Li,Xinyu Guo,Meidan Huo,Linjing Jia,Haiwei Xu,Hailing Qiao,Na Gao
Cytochrome P450 2E1 (CYP2E1) plays an important role in inflammatory disease, but the role of CYP2E1 in gout remains elusive. The pharmacokinetics in rats of Q11, a CYP2E1 inhibitor synthesized by our group, were measured. The anti-inflammatory and uric acid (UA)-lowering levels mechanism of Q11 was investigated in hyperuricemic (HUA) mice and acute gouty arthritis (AGA) rats, and in macrophage cells stimulated by monosodium urate (MSU). The half-life (t 1/2 ) of Q11 in the ankle joint and kidney was approximately 9 h, which was more than 4 times longer than that in plasma. The expression of CYP2E1 was increased in the kidneys of HUA mice and in the ankles of AGA rats. Q11 reduced plasma UA levels in HUA mice by decreasing xanthine oxidase (XOD) activity. In addition, Q11 also decreased renal inflammatory injury in HUA mice. Moreover, Q11 decreased the levels of IL-1β and TNF-α in AGA rats, increased the levels of catalase (CAT) and glutathione (GSH), and blocked the activation of the NLRP3 inflammasome. A similar phenomenon was observed in J774A.1 and THP-1 cells treated with sodium urate.CYP2E1 may be a new therapeutic target for the treatment of gout and Q11 has the dual effect of being anti-inflammatory and lowering UA. Significance Statement The specific cytochrome P450 2E1 (CYP2E1) inhibitor Q11 reduced plasma uric acid (UA) levels by decreasing xanthine oxidase (XOD) activity in a gout animal model, while attenuating joint and renal inflammatory injury through the inhibition of oxidative stress and NLRP3 inflammasome activation.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xingwei Wang,Heping Cui,Xiaoming Zhang,Jingyang Yu,Shuqin Xia,Chi-Tang Ho
Directional and rapid formation of the Amadori rearrangement product (ARP) from the glutamic acid and xylose was achieved through intermittent microwave heating. The yield of ARP reached 58.09 % by subjecting the system to intermittent microwave heating at a power density of 10 W/g for 14 min. Dehydration rate and microwave effects were found to be key factors to optimize the conditions for directional and rapid preparation of the ARP. Through a comprehensive analysis of the ARP degradation and further browning under both conductive and microwave thermal processing, it was observed that microwave processing significantly accelerated the browning degree of systems, leading to a tenfold reduction in the heating time required for browning. This research presented a promising avenue for the development of novel and expedited methods for the production of ARP and highlighted the potential of ARP in enhancing color quality in fast-cooking applications utilizing microwave.
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Heliyon
·
2024
·
IF 3.4
Shuo Li,Xiang-Yu Pei,Xin-Yi Liu,Shu-Liang Wang,Wen Xu,Jing-Jing Wang,Zhen Feng,Han Ding,Yin-Feng Zhang,Rui Zhang
MicroRNAs (miRNAs) are small noncoding RNAs of 18–25 bases. miRNAs are also important new biomarkers that can be used for disease diagnosis in the future. Studies have shown that miR-124 levels are significantly elevated during acute myocardial infarction (AMI) and play a key role in the cardiovascular system. A variety of methods have been established to detect myocardial infarction-related miRNAs. However, most require complex miRNA extraction and isolation, and these methods are virtually undetectable when RNA levels are low in the sample. It may lead to biased results. Thus, it is necessary to develop a technique that can detect miRNA without extracting it, which means that intracellular detection is of great significance. Here, we improved the traditional silicon spheres and obtained a biosensor that could effectively capture and detect specific noncoding nucleic acids through the layer-by-layer assembly method. The sensor is protected by hyaluronic acid so it can successfully escape the lysosome into the cell and achieve detection. With the help of a full-featured microplate reader, we determined that the detection limit of the biosensor could reach 1 fM, meeting the needs of intracellular detection. At the same time, we prepared an oxidative stress cardiomyocyte infarction model and successfully captured the overexpressed miR-124 in the infarcted cells to achieve in situ detection. This study could provide a new potential tool to develop miRNAs for sensitive diagnosis in AMI, and the proposed strategy implies its potential for biomedical research.
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Heliyon
·
2024
·
IF 3.4
Song Lin,Lixin Wen,Hong Zhao,Donghua Huang,Zuwei Yang,Qinge Zou,Ling Jiang
Since the p53 protein is an important promising biomarker of lung tumor and colorectal tumor, it is very essential to design a highly effective mean to monitor the degree of p53 for the early clinical analysis/therapy of the related tumors. In this work, a sandwich-type electrochemical immunosensing (SES) platform is proposed for the first time to detect p53 via synthesizing Ti 3 C 2 T x MXene nanoribbons (Ti 3 C 2 T x Nb) and ferrocene/gold nanoparticles (Fc/Au) respectively as the sensing substrate and signal-amplifier. The superior electrical property and large surface area of Ti 3 C 2 T x Nb are beneficial to assemble the initial p53-antibodies (Ab 1 ), while the synthesized Fc/Au is devoted to assemble the secondary p53 antibodies (Ab 2 ) and gives a magnified signal. By adopting the Fc molecules as the probes, the experiments reveal the response current of Fc resulted from the SES structure increases along with the p53 increase from 1.0 to 200.0 pg mL −1 . A considerable low detection limit (1.0 pg mL −1 ) is achieved after optimizing several key conditions, it is thus confirmed the as-proposed SES mean exhibits significant application in the detection of p53 protein and other targets.
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HYDROMETALLURGY
·
2024
·
IF 4.8
Huaixin Hao,Yaoyang Liu,Jianhua Yuan,Xue Dong,Zhipeng Wang,Chao Xu,Jing Chen
Cerium (Ce) has become an important strategic resource in high-tech fields owing to its excellent optical, magnetic, acoustic, and electrical properties. The recovery of non-natural Ce derived from nuclear fission through spent fuel reprocessing is of potential significance for the reservation of extremely precious resources. In the present work, Ce 3+ was oxidized to Ce 4+ by NaBiO 3 and then extracted by alkyl phosphate ligands. The extraction efficiency can be enhanced by increasing acidity, raising extractant concentration and decreasing temperature. By taking the advantage of the intrinsic selectivity to tetravalent lanthanides (Ln) by alkyl phosphates, highly efficient and selective extraction of Ce 4+ from other trivalent lanthanides with remarkable separation factor ( SF ) over 10 3 was achieved. Meanwhile, the back extraction of Ce could be accomplished through reducing Ce 4+ to Ce 3+ by H 2 O 2 . Further studies on structure-property relationship by spectroscopic methods and DFT calculations revealed that alkyl substituent groups with more C atoms enhanced the complexation between Ce 4+ and the ligand, while steric hindrance effect could weaken the extractability of Ce 4+ . Moreover, a shorter Ce O bond and a stronger covalent interaction in the Ce 4+ complex, as compared to the Ln 3+ complex, contributed to the excellent selectivity to Ce 4+ over Ln 3+ .
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BIOCHEMICAL PHARMACOLOGY
·
2024
·
IF 5.3
Shan Meng,Xin Chen,Jikai Zhao,Xinyi Huang,Yuting Huang,Tao Huang,Zijun Zhou,Wenpu Ren,Tao Hong,Jinfeng Duan,Liming Yu,Huishan Wang
Fibronectin type III domain-containing protein 5 (FNDC5) exerts potential anti-arrhythmic effects. However, the function and mechanism of FNDC5 in diabetes-associated atrial fibrillation (AF) remain unknown. In this study, bioinformatics analysis, in vivo and in vitro experiments were conducted to explore the alteration and role of FNDC5 in diabetes-related atrial remodeling and AF susceptibility. RNA sequencing data from atrial samples of permanent AF patients and diabetic mice exhibited significantly decreased FNDC5 at the transcriptional level, which was in line with the protein expression in diabetic mice as well as high glucose and palmitic acid (HG+PA) injured atrial myocytes. Diabetic mice exhibited adverse atrial remodeling and increased AF inducibility. Moreover, reduced atrial FNDC5 was accompanied with exacerbated NOD-like receptor pyrin domain containing 3 (NLRP3) activation and disturbed mitochondrial fission and fusion processes, as evidenced by decreased expressions of optic atrophy 1 (OPA-1), mitofusin (MFN-1, MFN-2) and increased phosphorylation of dynamin-related protein 1 (Ser616). These effects were validated in HG+PA-treated atrial myocytes. Critically, FNDC5 overexpression remarkably enhanced cellular antioxidant capacity by upregulating the expressions of superoxide dismutase (SOD1, SOD2) level. In addition, HG+PA-induced mitochondrial dysfunction was ameliorated by FNDC5 overexpression as evidenced by improved mitochondrial dynamics and membrane potential. Moreover, NLRP3 inflammasome-mediated inflammation was reduced by FNDC5 overexpression, and AMPK signaling might serve as the key down-stream effector. The present study demonstrated that reduced atrial FNDC5-AMPK signaling contributed to the pathogenesis of diabetes- associated AF by impairing mitochondrial dynamics and activating the NLRP3 inflammasome. These findings provide promising therapeutic avenues for diabetes-associated AF.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Chunte Feng,Hongfei Gao,Yang Yang,Xinyu Yang,Hao Tian,Huiyan Gu,Lei Yang
In this study, an acid-resistant magnetic boron-modified molecularly imprinted polymer (Fe 3 O 4 @NH 2 @BA@Lyz-MIPs) was prepared by the phase transition of lysozyme and used for the selective recognition and separation of rosmarinic acid from Perilla frutescens leaf extract. The synthesized materials were characterized by thermogravimetric analysis (TG), infrared spectroscopy (FT-IR), electron microscopy (SEM), and vibrating sample magnetometry (VSM). The adsorption and desorption experiments, adsorption kinetics experiments, and adsorption thermodynamics experiments revelated that the Fe 3 O 4 @NH 2 @BA@Lyz-MIPs exhibited a better adsorption capacity for rosmarinic acid (6.31 mg/g). Furthermore, Fe 3 O 4 @NH 2 @BA@Lyz-MIPs still exhibit a good adsorption performance after 8 adsorption-desorption cycles. The adsorption capacity decreased by 8.12 %, and the desorption performance decreased to 78.84 %. Stability experiments showed that the prepared imprinted layer was not destroyed by the eluent and could protect the internal magnetic core of the material. In summary, the prepared molecularly imprinted polymer Fe 3 O 4 @NH 2 @BA@Lyz-MIPs has good selective adsorption and high acid stability and can be swiftly retrieved from solution with the aid of an applied magnetic field, which provides a rapid and environmentally friendly strategy for the separation of rosmarinic acid from a complicated matrix.
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