LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Li Minglei,Wang Dingzhong,Zhao Wuduo,Xi Hui,Xu Hengyi,Sun Shihao,Fu Yingjie,Zhang Shusheng,Mao Jian,Zhang Jianxun
The excessive flavorings in food are a growing public health concern, particularly in foods for infants and children. A rapid and simple method for simultaneous detection of multiple flavorings in food is urgently needed. In this work, a method with direct analysis in real time-mass spectrometry has been developed and applied to the simultaneous determination of four flavorings, including maltol, ethyl maltol, vanillin, and ethyl vanillin. The ionization efficiency of flavor compounds in the quantitatively-dip-it probe mode was compared with that in the quick strip mode, which revealed that a solvent-assisted ionization mechanism might be involved in the ionization process. The developed method showed good linearity ( R 2 > 0.99) in the range from 5 to 1000 μg L −1 , sensitivity (LODs <3.3 μg L −1 ), recoveries (84.8%–96.9%), satisfactory repeatability (RSD <14.3%, n = 5), and high-throughout (12 infant formula samples completed in 15 min). The LOQs (0.55–1.1 mg kg −1 ) for the infant formula sample were much lower than the regulatory limits. The developed method provided an alternative way to monitor the excessive addition of flavorings.
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npj Science of Food
·
2026
·
IF 9.7
Zhang Luji,Li Hongxuan,Li Zimu,Li Xuejian,Cheng Shasha,Jiang Yujun,Man Chaoxin,Zhang Yu
Neonatal necrotizing enterocolitis (NEC) remains a major cause of morbidity and mortality in newborns, highlighting the need for safe nutritional interventions. We evaluated a prebiotic complex containing GOS, FOS, and LAU in combination with the human milk oligosaccharides 2′-FL and LNnT in a neonatal rat model of NEC. Forty-eight pups were allocated to eight groups ( n = 6 per group). Mix2-L, Mix2-H, and Mix2N-H increased baseline-adjusted body weight on D7–D9 relative to the model group, and the interventions improved colon morphology, histopathology, inflammatory and barrier-related measures, goblet-cell features, and mucin composition, with Mix2N-H showing the broadest response. Gut microbial community composition differed among groups (PERMANOVA pseudo-F = 3.819, R² = 0.455, P = 0.0001), and all eight cecal SCFAs showed significant overall group effects after FDR correction. In the 24 animals with exact microbiota–SCFA matching, Veillonella abundance was positively associated with butyrate (ρ = 0.663, q = 0.015) and propionate, whereas Ralstonia , Achromobacter , and Chryseobacterium showed negative associations with selected SCFAs. Mix2N-H also had higher butyrate than the model group. p-AKT/AKT and p-mTOR/mTOR differed among groups and were higher in several intervention groups than in the model group. These findings support a coordinated microbiota–SCFA–AKT/mTOR-associated response to prebiotic–HMO supplementation. These findings are based on a preclinical neonatal rat model.
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FOOD CHEMISTRY
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2023
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IF 8.8
Tiantian Fu,Hongwei Cao,Yu Zhang,Xiao Guan
To investigate the impact of milling on the active components in rice, this study examined the stability of phenols, vitamin B1 (VB1), and alpha-aminobutyric acid (α-GABA) during cooking and digestion of rice and their distribution in digestive juices and residue by adjusting the degree of milling (DOM). The findings revealed that milling exacerbated the instability of γ-GABA during cooking and VB1 during digestion. Their total losses peaked at 19.76% and 39.53% as DOM respectively reached 6.07% and 8.06%. In vitro digestion combined with release mathematical models demonstrated the impact of milling on their bioaccessibility. This effect increased the small intestinal bioaccessibility of phenols and γ-GABA, whilst reducing that of VB1. This was attributed to milling-induced pre-gastric full release of VB1, which amplifies its intestinal susceptibility and induces its re-entry into digestive residues. In conclusion, this study recommends keeping DOM below 6.07% to optimize the bioaccessibility of these active compounds.
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Animal Nutrition
·
2023
·
IF 6.3
Chao Zhu,Zifang Wu,Qimin Liu,Xiaolin Wang,Lijuan Zheng,Shuyang He,Fangxia Yang,Hong Ji,Wuzi Dong
The production of selenium-enriched fish can contribute to alleviating selenium deficiency in human diets. However, it is still unclear which selenium source, as an additive, can efficiently and cost-effectively produce high-quality selenium-enriched fish. This study evaluated the effects of selenium nanoparticles (SeNP), selenite, and selenomethionine (SeMet) on the growth, antioxidant capacity, selenium content, selenium speciation, and meat quality of grass carp. Ten diets were prepared, including a basal diet (BD) and three concentrations (0.1, 0.3, and 0.9 mg/kg) of SeNP, selenite, and SeMet. A total of 600 fish (250.79 ± 1.57 g) were randomly assigned to 30 tanks (3 tanks/group). Fish were fed the experimental diet three times daily for 60 d. In this study, SeNP most significantly promoted the growth and antioxidant capacity of grass carp, with 0.3 mg/kg SeNP identified as the optimal additive concentration. Additionally, SeNP demonstrated equally excellent bioavailability as SeMet and significantly increased the content of SeMet in grass carp ( Ctenopharyngodon idella ) muscle. Furthermore, compared to SeMet and selenite, dietary SeNP could more significantly enhance the content of selenocysteine (SeCys 2 ) and methylselenocysteine (MeSeCys) in grass carp muscle tissue. In addition, we have demonstrated that SeCys 2 and MeSeCys promote apoptosis of cancer cells (Hela) through the mitochondrial apoptotic pathway (involving Bax and Bcl-2). Furthermore, as an additive, 0.3 mg/kg SeNP significantly improved the flesh quality of grass carp by reducing crude fat and heavy metal content, as well as increasing the levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and the ratio of n-3/n-6 polyunsaturated fatty acid (PUFA). In summary, SeNP is the most suitable additive for producing selenium-enriched fish.
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JOURNAL OF INSECT PHYSIOLOGY
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2023
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IF 2.2
Wan-Xue Li,Jing-Xiang Chen,Chuan-Chuan Zhang,Min-Shi Luo,Wen-Qing Zhang
The brown planthopper (BPH, Nilaparvata lugens ), a major insect pest of rice, can make a shift in wing dimorphism to adapt to complex external environments. Our previous study showed that NlODC ( Ornithine decarboxylase in N. lugens ) was involved in wing dimorphism of the brown planthopper. Here, further experiments were conducted to reveal possible molecular mechanism of NlODC in manipulating the wing dimorphism. We found that the long-winged rate (LWR) of BPH was significantly reduced after RNAi of NlODC or injection of DFMO (D, L-α-Difluoromethylornithine), and LWR of males and females significantly decreased by 21.7% and 34.6%, respectively. Meanwhile, we also examined the contents of three polyamines under DFMO treatment and found that the contents of putrescine and spermidine were significantly lower compared to the control. After 3rd instar nymphs were injected with putrescine and spermidine, LWR was increased significantly in both cases, and putrescine was a little bit more effective, with 5.6% increase in males and 11.4% in females. Three days after injection of ds NlODC , injection of putrescine and spermidine rescued LWR to the normal levels. In the regulation of wing differentiation in BPH, NlODC mutually antagonistic to NlAkt may act through other signaling pathways rather than the classical insulin signaling pathway. This study illuminated a physiological function of an ODC gene involved in wing differentiation in insects, which could be a potential target for pest control.
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ANALYTICA CHIMICA ACTA
·
2026
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IF 6.1
Xi Bian,Kang Cheng,Hao Gao,Shaokai Jian,Zeyu Lin,Xinxin Zhang,Chengji Sui,Dafeng Jiang,Yongchao Lai
In-tube SPME-SERS platform enables rapid pollutant screening. • Dynamic flow enhances mass transfer and extraction capacity. • Sampling, enrichment, and detection are completed within 3 min. • The method successfully screens multiple hydrophobic pollutants in water. Background Given the persistence, bioaccumulation, and toxicity of hydrophobic pollutants (HPs), rapid and reliable screening methods are critically needed. Herein, we propose an integrated approach coupling surface-enhanced Raman spectroscopy (SERS) with in-tube solid-phase microextraction (in-tube SPME) for the rapid screening of HPs. The device integrates a dual-functional substrate (tert-dodecylthiol-modified silver nanostructures) within a quartz capillary and a syringe pump for flow control. During analysis, sample solutions are pumped through the capillary, enabling rapid HP adsorption onto the substrate via hydrophobic interactions under continuous flow. Results This dynamic configuration enhances mass transfer efficiency, facilitating rapid SERS detection with a portable Raman spectrometer. The entire “sampling and detection” workflow of the in-tube SPME-SERS method can be completed within 3 min. This method exhibits a good linear relationship (R 2 = 0.997) for the quantitative determination of Sudan III over the concentration range of 5–100 nM, with a limit of detection (LOD) as low as 0.658 nM, demonstrating outstanding sensitivity. Furthermore, the method shows satisfactory performance in terms of spatial homogeneity, batch-to-batch reproducibility, and long-term stability. Significance By balancing speed and accuracy in environmental HP analysis, this work provides a robust framework for portable screening platforms with significant potential for ecological monitoring and exposome research. Download: Download high-res image (211KB) Download: Download full-size image
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FOOD CONTROL
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2023
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IF 6
Yingming Duan,Yiquan Huang,Liling Huang,Zhangmin Xiang,Jian Liu,Shuqin Liu,Zhuo Chen
In this study, a nitrogen-rich triazine-based porous polymer (NTP) was prepared by the Schiff base mechanism. The NTP contains a high nitrogen content (35.47%) with a larger BET surface area and an adsorption average pore diameter of 860 m 2 g −1 and 3.92 nm, respectively. In addition, secondary amines (–C–NH–) and triazine nitrogen (–C N–) in NTP provide a large number of nitrogen active sites, which facilitate the adsorption of triazole pesticides. The synthesized NTP was then used as the solid phase microextraction (SPME) extraction phase material to fabricate the NTP-SPME fiber, which had significantly higher performance in extracting triazole fungicides compared to the commercial fibers ( p < 0.05). Subsequently, the bioaccumulation and elimination of three triazole fungicides (myclobutanil, propiconazole, and difenoconazole) in living vegetables were tracked by in vivo SPME sampling and quantified using a gas chromatography quadrupole time-of-flight mass spectrometry. The results showed that the concentrations of myclobutanil, propiconazole and difenoconazole were highest on the first day and showed a trend of first decreasing then equilibrium during bioaccumulation and gradually decreasing during elimination. The highest concentrations of myclobutanil, propiconazole, and difenoconazole in cabbage stems were 997.57 ± 51.42, 841.20 ± 21.42, and 56.82 ± 1.30 ng/g, respectively. In addition, concentrations of difenoconazole were much lower than those of other pesticides throughout the experimental period, which may be related to the difficulty of uptake by cabbage roots due to its strong hydrophobicity and low solubility. This study highlights the application of NTP-SPME for tracking triazole fungicides in living Chinese cabbage plants, which provides valuable information to rationale use of triazole fungicides and their ecological risk assessment.
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Nanomaterials
·
2023
·Vol. 13
·期 19
·2710
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IF 5.3
Haohao Cui,Ke Wang,Enhui Ma,Hong Wang
The ubiquitous pollution by antibiotics and heavy metal ions has posed great threats to human health and the ecological environment. Therefore, we developed a self-propelled tubular micromotor based on natural fibers as an active heterogeneous catalyst for antibiotic degradation and adsorbent for heavy metal ions in soil/water. The prepared micromotors can move in the presence of hydrogen peroxide (H2O2) through a bubble recoil mechanism. The MnO2NPs and MnFe2O4NPs loaded on the hollow fibers not only enabled self-driven motion and magnetic control but also served as activators of peroxymononsulfate (PMS) and H2O2to produce active free radicals SO4•−and •OH. Benefiting from the self-propulsion and bubble generation, the micromotors can effectively overcome the disadvantage of low diffusivity of traditional heterogeneous catalysts, achieving the degradation of more than 90% TC in soil within 30 min. Meanwhile, due to the large specific surface area, abundant active sites, and strong negative zeta potential, the micromotors can effectively adsorb heavy metal ions in the water environment. In 120 min, self-propelled micromotors removed more than 94% of lead ions, an increase of 47% compared to static micromotors, illustrating the advantages of on-the-fly capture. The prepared micromotors with excellent catalytic performance and adsorption capacity can simultaneously degrade antibiotics and adsorb heavy metal ions. Moreover, the magnetic response enabled the micromotors to be effectively separated from the system after completion of the task, avoiding the problem of secondary pollution. Overall, the proposed micromotors provide a new approach to the utilization of natural materials in environmental applications.Keywords:micromotors;biotemplated fabrication;water/soil treatment;antibiotics;heavy metal ions
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FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Biao Liu,Ye-hui Liang,Yang-Zheng He,Wenhui Ye,Ze-Yuan Deng,Jing Li,Shuntang Guo
The differences in the milk fat digestion from goat milk (GM), camel milk (CM), bovine milk (BM), sheep milk (SM), mare milk (MM) and human milk (HM) using an in vitro gastrointestinal digestion model for simulated infants were investigated. The particle size distributions in goat and mare milk were similar to that of HM after digestion in the small intestine. During in vitro digestion, the zeta-potential change of MM was more consistent with that of HM. After 60 min of gastric digestion, the lipolysis degree (LD) of different milks were<2%, of which the highest LD was MM (1.84%), followed by HM (1.45%). At the end of intestinal digestion, the LD of HM was the highest, reaching 88.47%, and the LD of SM was similar to that of HM, reaching 83.92%, followed by GM (57.00%), BM (40.98%) and MM (39.37%), respectively, the LD of CM was only 29.99%, which was much lower than HM. The results of the glyceride composition hierarchical clustering analysis revealed that MM and HM were clustered into one category at the end of gastric and intestinal digestion. This study provides a scientific basis for the development of lipid ingredients in infant formula.
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NEUROPSYCHOBIOLOGY
·
2023
·
IF 3.2
Cao Ting,Wang LiWei,Jiao ShiMeng,Chen Hui,Lin ChenQuan,Zhang BiKui,Cai HuaLin
Introduction: Progesterone receptor component 1 (PGRMC1) has been identified as a potential target in atypical antipsychotic drug-induced metabolic disturbances as well as neuroprotection in the central nervous system. In our study, we aimed to figure out the essential role of PGRMC1 signaling pathway underlying clozapine-induced cognitive impairment. Methods: In male SD rats, we utilized recombinant adeno-associated viruses (BBB 2.0) and the specific inhibitor of PGRMC1 (AG205) to regulate the expression of PGRMC1 in the brain, with a special focus on the hippocampus. Treatments of clozapine and AG205 were conducted for 28 days, and subsequent behavioral tests including modified elevated plus maze and Morris water maze were conducted to evaluate the cognitive performance. Hippocampal protein expressions were measured by Western blotting. Results: Our study showed that long-term clozapine administration led to cognitive impairment as confirmed by behavioral tests as well as histopathological examination in the hippocampus. Clozapine inhibited neural survival through the PGRMC1/EGFR/GLP1R-PI3K-Akt signaling pathway, leading to a decrease in the downstream survival factor, brain-derived neurotrophic factor (BDNF), and simultaneously promoted neural apoptosis in the rat hippocampus. Intriguingly, by targeting at the hippocampal PGRMC1, we found that inhibiting PGRMC1 mimics, while its upregulation notably mitigates clozapine-induced cognitive impairment through PGRMC1 and its downstream signaling pathways. Conclusion: PGRMC1-overexpression could protect hippocampus-dependent cognitive impairment induced by clozapine. This effect appears to arise, in part, from the upregulated expression of PGRMC1/EGFR/GLP1R and the activation of downstream PI3K-Akt-BDNF and caspase-3 signaling pathways
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ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 44
·2306466
·
IF 19
Li Wang,Yukun Li,Yinyin Ai,Erchuang Fan,Fei Zhang,Wentao Zhang,Guosheng Shao,Peng Zhang
Photocatalytic conversion of H 2 O and CO 2 into solar fuels is considered to be a green and renewable technology while photocatalytic performance is hampered by the severe recombination of photogenerated electron–hole pairs. Drawing inspiration from the concepts of the electrons transfer layer (ETL) and the holes transfer layer (HTL) in perovskite solar cells, a multicomponent photocatalyst SrTiO 3 /NiO/NiS that incorporates p–n heterojunction and Schottky junction is constructed for converting solar energy into easily storable solar fuels. The NiS and NiO play the roles analogy to the ETL and the HTL which accomplishes the reverse migration of electrons and holes, accordingly, SrTiO 3 /NiO/NiS exhibits a noticeable enhancement in photocatalytic performance. However, the lack of direct observation of charge transfer pathways in complex multicomponent photocatalysts makes it challenging to thoroughly investigate their catalytic mechanisms. Herein, the in situ irradiation X-ray photoelectron spectroscopy (ISI–XPS) is employed to track photogenerated charges migration pathways between microscopic heterogeneous interfaces, thereby providing a comprehensive elucidation of the catalytic mechanism and heterojunction formation process by integrating the photoelectrochemical tests. This study not only serves as compelling evidence for the capability of ISI–XPS in directly and accurately tracking charge transfer pathways but also as an intriguing highlight within the catalyst design strategy.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Long Chen,Guihua Hu,Bo Cui,Jinming Gao,Xiaofeng Gu,Haixin Cui,Zhanghua Zeng
The detection of microRNA (miRNA), a gene-regulating factor, is crucial for predicting plant growth and development. In this study, we successfully developed a fluorescent nanosensor using nanomaterials and molecular beacons for in situ miRNA detection in plant cells. The molecular beacons were loaded onto 15 nm gold nanoparticles , effectively protecting them from DNase I degradation. The nanosensor exhibits remarkable specificity and detection capability with a detection limit of 1.0 nM, while maintaining stability in plant nutrient solutions. It is readily absorbed and transported by plant through both the symplasmic pathway and apoplastic pathway. Furthermore, it efficiently converts miRNA signals into fluorescent signals, providing valuable insights into the spatial and temporal distribution of miRNA in plants. Although to achieve more accurate detection, the detection limit of the designed nanosensor still needs to be further improved, but this technique offers a convenient and efficient approach, serving as a powerful tool for studying the mechanisms and regulation in plants.
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