FOOD CHEMISTRY
·
2024
·
IF 8.5
Liushan Jiang,Guangyu Dong,Denghao Song,Wenjing Liu,Xiaodie Geng,Dejing Meng,Linchun Nie,Jiawei Liao,Qingxiang Zhou
In this study, a hydrophobic covalent organic framework-functionalized magnetic composite (CoFe 2 O 4 @Ti 3 C 2 @TAPB−TFTA) with a high specific area with 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 2,3,5,6-tetrafluoroterephthalaldehyde (TFTA) was designed and synthesized through Schiff base reaction. An efficient magnetic solid-phase extraction method was established and combined with gas chromatography–triple quadrupole mass spectrometry to sensitively determine 10 organochlorine and organophosphorus pesticides in tea samples. The established method exhibited good linearity in the range of 0.05–120 μg/L and had low limits of detection (0.013–0.018 μg/L). The method was evaluated with tea samples, and the spiked recoveries of pesticides in different tea samples reached satisfactory values of 85.7–96.8%. Moreover, the adsorption of pesticides was spontaneous and followed Redlich–Peterson isotherm and pseudo-second-order kinetic models. These results demonstrate the sensitivity, effectiveness, and reliability of the proposed method for monitoring organochlorine and organophosphorus pesticides in tea samples, providing a preliminary basis for researchers to reasonably design adsorbents for the efficient extraction of pesticides.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Wei Jia,Yuyang Liu,Lin Shi,Xiaogang Chu
Se-enriched green tea, with an increasing consumption, is the shoot of tea plants grown naturally in a seleniferous region. A label-free proteomic strategy based on ultrahigh-performance liquid chromatography quadrupole Orbitrap was applied to characterize and distinguish the difference between the Se-enriched and normal green tea with a total of 283 proteins identified and 264 proteins quantified, in which 96 proteins were observed different. The expressions of 10 proteins were upregulated and 40 proteins were downregulated (p < 0.05) in Se-enriched samples. Gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and protein–protein interaction (PPI) network analysis results indicated that these differentially expressed proteins significantly interacted and were involved in secondary metabolites and inflammatory response biological processes. Furthermore, the expression of methyl-jasmonate- and ethylene-related genes changed significantly in Se-enriched green tea, and catalase proteins were employed as the center of the pathway that changed significantly in the PPI network. These results associating with the current knowledge of selenium in soil–plant cycling revealed that organic selenium was synthesized in green tea, which provided novel information on Se assimilation in Camellia sinensis and improved the understanding of Se-enriched green tea as a possible ideal selenium supplement in daily life.
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Food Bioscience
·
2025
·
IF 5.9
Aikebaier Jumai,Shaohua Chen,Yaodan Wu,Fen Liu,Bailin Li,Baojun Zhu,Liyun Zhao,Kanglun Liu,Qingzhong Zhang,Sheng-Xiang Qiu
Metabolic disorders like obesity and dyslipidemia are largely driven by high-fat diets (HFD), often with limited efficacy from current dietary interventions. This study investigates Swertia diluta , an edible potherb, for its potential hepatic and metabolic benefits. Using HPLC-MS/MS, 25 xanthone compounds were identified, with network pharmacology analysis indicating their relevance to lipid metabolism and atherosclerosis. Notably, bellidifolin (BEL) was isolated from this plant and studied in 12-week HFD-induced obesity model (HFD feeding for 12 weeks; BEL intervention from weeks 9–12), demonstrating significant improvements in lipid profiles and body weight reduction in male C57BL/6J mice. BEL also altered gut microbiota by reducing Firmicutes , Lactobacillaceae , and Clostridiaceae abundance. Additionally, BEL enhanced bile acid biosynthesis and excretion, further improving lipid metabolism. These findings suggest BEL from Swertia diluta as a promising natural agent for weight management and enhanced lipid metabolism through gut microbiota and bile acid modulation in HFD conditions.
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Food Chemistry-X
·
2025
·
IF 8.2
Libing Zhou,Chunli Huang
This study employed a range of indicators, including heat of combustion, combustibility, fat content, amino acid content, ash content, crude fiber content, trace element content, and concentrations of catechins, theanine, and caffeine, to develop comprehensive evaluation systems for assessing the quality of five tea samples: Duyun Maojian tea, Liping fragrant tea, Shuicheng spring tea, Huaxi jasmine tea, and Zunyi black tea. Analytical techniques such as gray pattern recognition (GPR), gray principal component analysis (GPCA), gray factor analysis (GFA), entropy analysis (EA), and factor cluster analysis (FCA) were utilized. Based on the 42variable data, the nutritional value ranked as follows: Shuicheng spring tea > Huaxi jasmine tea > Duyun Maojian tea > Liping fragrant tea > Zunyi black tea. These findings offer significant theoretical and practical insights for the determination and comprehensive evaluation of tea quality indicators, contributing to scientific research on the nutrition, health, and classification aspects of tea.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Kaige Zhang,Yunhe Wang,Shuangying Li,Guifen Zhu
Deep eutectic solvents (DESs) are a new class of green “designer solvent”; its physicochemical properties can be easily tuned by adjusting DES′ constituents, chemical ratio and water content. In this study, three hydrophobic DESs with low viscosity, low density, and melting points close to room temperature were designed and synthesized. Based on these DESs , an air-assisted liquid–liquid microextraction technique was developed based on the solidification of floating DESs for the simultaneous determination of bisphenols and polycyclic aromatic hydrocarbons (PAHs) via HPLC. The microextraction parameters were optimized via the Plackett-Burman design and response surface methodologies. The method shows satisfactory linearity (R 2 ≥ 0.9928), a low limit of detection (0.16–0.75 μg L −1 ) and satisfactory precision (≤2.3%), and was successfully applied for the simultaneous determination of bisphenols and PAHs from tea infusions with satisfactory recoveries (82.0–116.6%). This method is simple, rapid, economical, environmentally compatible, dispersive solvent-frees and centrifugation-free, and has promising applications in food safety.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yingying Li,He Zhou,Tian Tian,Yihong Hou,Dan Chen,Jie Zhou,Shuyuan Liu,Youben Yu,Weidong Dai,Tianshan Zhou
To investigate the effects of “golden flora” amount on the sensory quality, metabolites and bioactivities of Fu brick tea (FBT), FBT samples with different “golden flora” amounts were prepared from the same materials by adjusting the water content before pressing. With the increase of “golden flora” in samples, the tea liquor color changed from yellow to orange red and the astringent taste gradually diminished. Targeted analysis demonstrated that (−)-epigallocatechin gallate, (−)-epicatechin gallate, and most amino acids gradually decreased as the increase of “golden flora”. Seventy differential metabolites were identified by untargeted analysis. Among them, sixteen compounds including two Fuzhuanins and four EPSFs were positively correlated with “golden flora” amount ( P < 0.05). The FBT samples with “golden flora” exhibited significantly higher inhibitory potency on α-amylase and lipase than the samples without “golden flora”. Our results provide a theoretical basis of guiding FBT processing based on desired sensory quality and metabolites.
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Analytical Methods
·
2024
·Vol. 16
·期 16
·2513-2521
·
IF 2.7
Zhengming Qian,Qinggui Lei,Dan Tang,Guoying Tan,Qi Huang,Fucai Zhou,Wenhao Wang
Sweet tea is a functional herbal tea with anti-inflammatory, anti-diabetic, and other effects, in which phloridzin and trilobatin are two functional compounds. However, the current methods for their quantification are time-consuming, costly, and environmentally unfriendly. In this paper, we propose a rapid method that integrates online pressurized liquid extraction and high-performance liquid chromatography featuring a superficially porous column for fast separation. Moreover, we employ an equal absorption wavelength method to eliminate using multiple standard solutions and relative calibration factors. Our verification process corroborated the technique's selectivity, accuracy, precision, linearity, and detection limitations. Separately, our methodology demonstrated excellent analytical efficiency, cost-effectiveness, and environmental friendliness. Practical application using six distinct batches of sweet tea samples yielded results in congruence with the external standard method. The analytical rate of this technique is up to over 18 times faster than traditional methods, and organic solvent consumption has been reduced to less than 1.5 mL. Therefore, this method provides a valuable way to achieve quality control and green analysis of sweet tea and other herbal teas.
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PHYTOPATHOLOGY
·
2023
·
IF 3.2
Mr. Jiayu Yin,Ms. Shuang Wu,Ms. Yongli Yang,Dr. Delu Wang,Prof. Yue Ma,Dr. Yongtian Zhao,Prof. Sujitraj Sheth,Miss Honglin Huang,Dr. Baoan Song,Dr. Zhuo Chen
Carvacrol expresses a wide range of biological activities, but the studies of its mechanisms focused on bacteria, mainly involving the destruction of the plasma membrane. In this study, carvacrol exhibited strong antifungal activities against several phytopathogenic fungi and determined a novel antifungal mechanism against Lasiodiplodia theobromae. RNA sequencing indicated that many genes of L. theobromae hyphae were predominately induced by carvacrol, particularly those involved in replication and transcription. Hyperchromic, hypsochromic, and bathochromic effects in the UV-visible absorption spectrum were observed following titration of calf thymus DNA (ctDNA) and carvacrol, which indicated the formation of a DNA–carvacrol complex. Circular dichroism (CD) spectroscopy indicated the response of DNA to carvacrol was similar to 4’, 6-diamidino-2-phenylindole (DAPI), but different from that of ethidium bromide (EB), implying the ionic bonds between carvacrol and ctDNA. Fluorescence spectrum (FS) analysis indicated that carvacrol quenched the fluorescence of double-stranded DNA more than single-stranded DNA, indicating that carvacrol mainly bound to double-stranded DNA. A displacement assay showed that carvacrol reduced the fluorescence intensity of the DNA–DAPI complex through competing with DAPI, but this did not occur for DNA–EB. FS assay revealed that carvacrol bound to AAA sequence on the minor groove of ds-oligonucleotides. The hydroxyl of carvacrol was verified to bind to ctDNA through a comparative test in which structural analogs of carvacrol, including thymol, 4-ethyl-1,2-dimethyl, etc. were analyzed. The current study indicated carvacrol can destruct plasma membranes and bind to the minor groove of DNA, which inhibited the fungal proliferation by disturbing the stability of dsDNA.
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2021
·Vol. 59
·期 6
·510-520
·
IF 1.618
Xue Ying,Wang Ziteng,Cai Weimin,Tian Xin,Liu Shuaibing
Ticagrelor is recommended for management of patients with acute coronary syndromes. Green tea is one of the most popular beverages in China and around the world. Their concomitant use is unavoidable. In this study, a selective and sensitive liquid chromatography–tandem mass spectrometry method for the simultaneous determination of plasma concentrations of ticagrelor, its two metabolites and four major constituents of tea polyphenols (TPs) in rats was developed for co-administration study of ticagrelor and TPs. Diazepam was used as internal standard (IS). Plasma samples were extracted employing a liquid–liquid extraction technique. Chromatographic separation was carried out on a Kinetex C18 column (2.1 × 75 mm, 2.6 μm) by gradient elution using 0.1% formic acid in water, acetonitrile and methanol. Seven analytes and IS were detected by a mass spectrometer with both positive and negative ionization by multiple reaction monitoring mode. The method was fully validated to be reliable and reproducible in accordance with food and drug administration (FDA) guidelines on bioanalytical method validation. The method was then successfully applied for pharmacokinetic study of ticagrelor, its two metabolites and four major constituents of TPs in rat plasma after oral administration of ticagrelor and tea polyphenol extracts.
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FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Yanqin Yang,Jinjie Hua,Yuliang Deng,Yongwen Jiang,Michael C. Qian,Jinjin Wang,Jia Li,Mingming Zhang,Chunwang Dong,Haibo Yuan
The drying technology is crucial to the quality of Congou black tea. In this study, the aroma dynamic characteristics during the variable-temperature final firing of Congou black tea was investigated by electronic nose (e-nose) and comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC-TOFMS). Varying drying temperatures and time obtained distinctly different types of aroma characteristics such as faint scent, floral aroma, and sweet fragrance. GC × GC-TOFMS identified a total of 243 volatile compounds. Clear discrimination among different variable-temperature final firing samples was achieved by using partial least squares discriminant analysis (R2Y = 0.95, Q2 = 0.727). Based on a dual criterion of variable importance in the projection value (VIP > 1.0) and one-way ANOVA ( p < 0.05 ), ninety-one specific volatile biomarkers were identified, including 2,6-dimethyl-2,6-octadiene and 2,5-diethylpyrazine with VIP > 1.5. In addition, for the overall odor perception, e-nose was able to distinguish the subtle difference during the variable-temperature final firing process.
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MOLECULES
·
2018
·Vol. 23
·期 7
·1554
·
IF 3.098
Mijun Peng,Huan Li,Ruiqing Long,Shuyun Shi,Hanjun Zhou,Shuping Yang
Magnetic porous molecularly imprinted polymers (MPMIPs) for rapid and efficient selective recognition of chlorogenic acid (CGA) were effectively prepared based on surface precipitation polymerization using CGA as template, 4-vinylpyridine (4-VP) as functional monomer, and mesoporous SiO2(mSiO2) layer as sacrificial support. A computational simulation by evaluation of electronic binding energy is used to optimize the stoichiometric ratio between CGA and 4-VP (1:5), which reduced the duration of laboratory trials. The porous MIP shell and the rid of solid MIPs by magnet gave MPMIPs high binding capacity (42.22 mg/g) and fast kinetic binding (35 min). Adsorption behavior between CGA and MPMIPs followed Langmuir equation and pseudo-first-order reaction kinetics. Furthermore, the obtained MPMIPs as solid phase adsorbents coupled with high performance liquid chromatography (HPLC) were employed for selective extraction and determination of CGA (2.93 ± 0.11 mg/g) in Duzhong brick tea. The recoveries from 91.8% to 104.2%, and the limit of detection (LOD) at 0.8 μg/mL were obtained. The linear range (2.0–150.0 μg/mL) was wide withR2> 0.999. Overall, this study provided an efficient approach for fabrication of well-constructed MPMIPs for fast and selective recognition and determination of CGA from complex samples.Keywords:surface precipitation polymerization;mesoporous SiO2;selective extraction;chlorogenic acid;Duzhong brick tea
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shan Zhang,Qinyan Yu,Linchi Niu,Haibo Yuan,Xujiang Shan,Jinjie Hua,Le Chen,Qianting Zhang,Yuning Feng,Xiaolan Yu,Qinghua Zhou,Yongwen Jiang,Jia Li
First-drying is a crucial step in black tea processing. Herein, the influence of different first-drying methods on black tea flavor formation was investigated, including box-hot air first-drying (BFD), roller first-drying, and microwave first-drying. Electronic tongue and color difference revealed distinct taste profiles (especially in bitter, astringency, sweet, umami) and liquor colors among three groups. Quantification and metabolomics analysis revealed that tea pigments (theaflavins, thearubigins), catechins, and other 34 metabolites including dimeric/trimeric catechins, amino acids and derivatives, flavonols and flavonol/flavone glycosides, phenolic acids, etc., were key differential components. The evolution of key metabolites, polyphenol oxidase (PPO) and peroxidase (POD) activities were tracked during drying. BFD exhibited significantly slower enzyme inactivation rate. Multiple conversions were possibly involved in drying, including catechins conversion (polymerization, degalloylation, epimerization), hydrolysis of flavonol- O -glycosides and phenolic acid esters, flavone- C -glycosides synthesis, etc., driven by the remaining PPO and POD activities and heat. Moreover, validation batch further verified the result.
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