Acta Geotechnica
·
2023
·Vol. 18
·期 10
·5299-5318
·
IF 5.7
Zhao Chang,Xiao Yang,Chu Jian,Hu Ran,Liu Hanlong,He Xiang,Liu Yi,Jiang Xiang
Enzymatically induced carbonate precipitation (EICP) in heterogeneous subsurface is of interest in various geoscience and environmental applications. Through fabricating microfluidic cells with different pore networks, the pore-scale formation of biological CaCO 3 was investigated experimentally by transverse mixing using time lapse high-resolution camera. The precipitation pattern over time was processed to obtain the evolution of distribution of CaCO 3 volume fraction. The impact of flow rate and pore-scale heterogeneity were quantitatively evaluated with reaction index including overall pore filling ratio, precipitation rate and precipitation efficiency. The results showed that low flow rates and strong heterogeneity in porous media are the two favorable conditions for precipitation process due to more nucleation sites. At grain scale, a statistic of three-dimensional morphologies of individual crystals was evaluated with specific surface area and degree of anisotropy. Localized precipitates in multiple pores were adopted as a representative of the whole porous media. The analysis showed that the complex pore structures are generated due to precipitates in pores, thus limiting transport of reactants and result in the permeability reduction. We furthermore confirmed different polymorphs of calcium carbonate, mainly containing vaterite and calcite. Pore-scale analysis of biological CaCO 3 in porous media significantly contributes to the understanding of advection–diffusion-reaction coupling effect, and further revealing the role of pore network on biomineralization process.
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Carbon Letters
·
2023
·Vol. 33
·期 6
·1811-1817
·
IF 4.5
Chen Junnan,Yu Lingmin,Xu Wenzhen,Lin Tao,Jiang Sicong,Jin Caijin
Graphene-based sensors have emerged as significant tools for biosensing applications due to their unique electrical, mechanical, and thermal properties. In this study, we have developed an innovative and sensitive aptasensor based on the surface-modified graphene for the detection of lung cancer biomarker CA125. The sensor leverages the combination of graphene surface and gold nanoparticles (AuNPs) electrodeposition to achieve a high level of sensitivity and selectivity for the biomarker detection. A noticeable decrease in electron transfer resistance was observed upon the AuNPs deposition, demonstrating the enhancement of electrochemical performance. Our experimental findings showed a strong linear relationship between the sensor response and CA125 concentrations, ranging from 0.2 to 15.0 ng/mL, with a detection limit of 0.085 ng/mL. This study presents a novel approach to lung cancer detection, surpassing the traditional methods in terms of invasiveness, cost, and accuracy. The results from this work could pave the way for the development of graphene-based sensors in various other biosensing applications.
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RESPIRATORY RESEARCH
·
2023
·Vol. 24
·期 1
·1-18
·
IF 5.8
Wu Zhuhua,Zhu Li,Nie Xinran,Liu Yingli,Zhang Xiaoju,Qi Yong
Background Obesity has been identified as a risk factor for acute lung injury/acute respiratory distress syndrome (ALI/ARDS). However, the underlying mechanisms remain elusive. This study aimed to investigate the role of fatty acid synthase (FASN) in lipopolysaccharide (LPS)-induced ALI under obesity. Methods A high-fat diet-induced obese (DIO) mouse model was established and lean mice fed with regular chow diet were served as controls. LPS was intratracheally instilled to reproduce ALI in mice. In vitro, primary mouse lung endothelial cells (MLECs), treated by palmitic acid (PA) or co-cultured with 3T3-L1 adipocytes, were exposed to LPS. Chemical inhibitor C75 or shRNA targeting FASN was used for in vivo and in vitro loss-of-function studies for FASN. Results After LPS instillation, the protein levels of FASN in freshly isolated lung endothelial cells from DIO mice were significantly higher than those from lean mice. MLECs undergoing metabolic stress exhibited increased levels of FASN, decreased levels of VE-cadherin with increased p38 MAPK phosphorylation and NLRP3 expression, mitochondrial dysfunction, and impaired endothelial barrier compared with the control MLECs when exposed to LPS. However, these effects were attenuated by FASN inhibition with C75 or corresponding shRNA. In vivo, LPS-induced ALI, C75 pretreatment remarkably alleviated LPS-induced overproduction of lung inflammatory cytokines TNF-α, IL-6, and IL-1β, and lung vascular hyperpermeability in DIO mice as evidenced by increased VE-cadherin expression in lung endothelial cells and decreased lung vascular leakage. Conclusions Taken together, FASN inhibition alleviated the exacerbation of LPS-induced lung injury under obesity via rescuing lung endothelial dysfunction. Therefore, targeting FASN may be a potential therapeutic target for ameliorating LPS-induced ALI in obese individuals.
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Biomaterials Research
·
2023
·Vol. 27
·期 1
·1-17
·
IF 11.3
Huang Xueyan,Chen Fei,Ma Yeshuo,Zheng Fan,Fang Yanpeng,Feng Bin,Huang Shuai,Zeng Hongliang,Zeng Wenbin
Background Macroautophagy is an essential cellular self-protection mechanism, and defective autophagy has been considered to contribute to a variety of diseases. During the process, cytoplasmic components are transported via autophagosomes to acidic lysosomes for metabolism and recycling, which represents application niches for lysosome-targeted fluorescent probes. Additionally, in view of the complexity of the autophagy pathway, it entails more stringent requirements for probes suitable for monitoring autophagy. Meanwhile, aggregation-induced emission (AIE) fluorescent probes have been impressively demonstrated in the biomedical field, which bring fascinating possibilities to the autophagy visualization.Methods We reported a generalizable de novo design of a novel pH-sensitive AIE probe ASMP-AP tailored to lysosome targeting for the interpretation of autophagy. Firstly, the theoretical calculation was carried out followed by the investigation of optical properties. Then, the performance of ASMP-AP in visualizing autophagy was corroborated by starvation or drugs treatments. Furthermore, the capability of ASMP-AP to monitor autophagy was demonstrated in ex vivo liver tissue and zebrafish in vivo.Results ASMP-AP displays a large stokes shift, great cell permeability and good biocompatibility. More importantly, ASMP-AP enables a good linear response to pH, which derives from the fact that its aggregation state can be manipulated by the acidity. It was successfully applied for imaging autophagy in living cells and was proved capable of monitoring mitophagy. Moreover, this novel molecular tool was validated by ex vivo visualization of activated autophagy in drug-induced liver injury model. Interestingly, it provided a meaningful pharmacological insight that the melanin inhibitor 1-phenyl-2-thiourea (PTU)-induced autophagy was clearly presented in wild-type zebrafish.Conclusion sASMP-AP offers a simple yet effective tool for studying lysosome and autophagy. This is the first instance to visualize autophagy in zebrafish using a small-molecule probe with AIE characters, accurate lysosome targeting and simultaneous pH sensitivity. Ultimately, this novel fluorescent system has great potential for in vivo translation to fuel autophagy research.Graphical Abstract
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JOURNAL OF POLYMER RESEARCH
·
2023
·Vol. 30
·期 4
·1-10
·
IF 2.8
Li Tao,Zhang Yang,Sun Yu,Dai Haiyang,Chen Jing
Ultra-low permittivity ( κ ) materials have broad application prospects in the microelectronic fields. In this study, we prepared microporous polyimide-based composite membranes with ultra-low permittivity by incorporating a trace of cage-like Phenyl-POSS (POSS) into fluorinated polyamic acid (PAA).The effects of POSS incorporation on their dielectric properties correlated with the characteristics of the free volume defects of composite membranes have been investigated by positron annihilation technique. Incorporating POSS with hollow core into PI can effectively improve the free volume size and the relative volume fraction of composite membranes, which optimizes their low dielectric properties, but reduces their dielectric breakdown strength. The membrane with 3 wt % POSS presents an ultra-low permittivity ( κ = 1.77 at 10 4 Hz) and maintains excellent mechanical properties . As POSS-doping amount reaches 5wt% , POSS agglomerates in composite membranes, resulting in a rebound in its permittivity. The incorporation of POSS reduces the tensile strength of composite membranes, which can be attributed to the fact that the rigid phenyl-POSS dispersing in the matrix weakens the interaction between the molecular chains of PI. The experimental results can provide valuable information for improving the dielectric properties of polyimide-based membranes in future.
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 3
·1-14
·
IF 2.9
Wu Jian,Jiang Hao,Liu Shengxue,Yang Desong,Cui Jianghu
The removal of Cd from irrigation water and the remediation of Cd-contaminated soil remain significant challenges. Herein, we developed necklace-like core-shell structured mesoporous silica-coated nanoscale zero-valent iron (NL-NZVI@mHs) to efficiently remediate Cd-contaminated water and soil. The obtained materials had a large specific surface area and a higher adsorption capacity of 41.38 mg/g on Cd(II) [this was 5.7 times for nano-zero-valent iron (NZVI) alone]. X-ray photoelectron spectroscopy demonstrated that ligand exchange, surface complexation, and electrostatic adsorption were the main mechanisms of Cd(II) adsorption. In addition, NL-NZVI@mHs exhibited good performance in the removal of Cd(II) from practical irrigation water samples. The nanochain mesoporous term chain of zero-valent iron exhibits long-term stability, indicating that it can be used multiple times (reduce waste volume). Furthermore, the addition of the obtained NL-NZVI@mHs composites decreased the bioavailability of Cd in the soil and significantly reduced the Cd content in rice seedlings. The results of this study provide a promising strategy for the remediation of Cd-polluted water and soil that may have large-scale applications in the future. Graphical abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Wu Jine,Liao Chenyi,Li Tianyu,Zhou Jing,Zhang Linjuan,Wang Jian-Qiang,Li Guohui,Li Xianfeng
Membranes with fast and selective ion transport are essential for separations and electrochemical energy conversion and storage devices. Metal-coordinated polymers are promising for fabricating ion-conducting membranes with molecular channels, however, the structures and ion transport channels remain poorly understood. Here, we reported mechanistic insights into the structures of metal-ion coordinated polybenzimidazole membranes and the preferential K + transport. Molecular dynamics simulations suggested that coordination between metal ions and polybenzimidazole expanded the free volume, forming subnanometre molecular channels. The combined physical confinement in nanosized channels and electrostatic interactions of membranes resulted in a high K + transference number up to 0.9 even in concentrated salt and alkaline solutions. The zinc-coordinated polybenzimidazole membrane enabled fast transport of charge carriers as well as suppressed water migration in an alkaline zinc-iron flow battery, enabling the battery to operate stably for over 340 hours. This study provided an alternative strategy to regulate the ion transport properties of polymer membranes by tuning polymer chain architectures via metal ion coordination.
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Journal of Food Measurement and Characterization
·
2023
·
IF 3.4
Jie Mingsha,Lan Shengkai,Lu Congran,Guo Ruipeng,Qu Xuanxuan,Zheng Qiuchen,Xiang Qisen
Considering the excessive residue and serious harm of sulfamethazine (SM 2 ), the sensitive determination of SM 2 has attracted wide attention in ensuring food security and human health. Herein, using the unique fluorescence property of aggregation-induced emission (AIE), we reported a simple and sensitive detection approach of SM 2 residues in milk and drinking water. Firstly, luminol-europium nanocomposites (luminol-Eu NCs) were self-assembly synthesized via AIE strategy. Then, the fluorescence emission of luminol-Eu NCs could be quenched by SM 2 due to the inner filter effect (IFE). As a result, the fluorescent sensor based on luminol-Eu NCs displayed a wide linear response for SM 2 in a concentration range of 0.1–500 µmol/L and a detection limit of 90 nmol/L was obtained. Furthermore, semi-quantitative detection of SM 2 on test papers was realized via a smartphone-assisted visual sensing device. Therefore, this fluorescent sensor shows a potential value in the field of food safety assessment. Graphical abstract
An aggregation-induced emission fluorescent
sensor based on luminol-europium nanocomposites was developed and applied for
sensitive and visual detection of sulfamethazine in milk and drinking water.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 23
·64352-64362
·
IF 5.8
Qian Cheng,Ma Jie,Wu Qiong
As a typical carbamate pesticide, methomyl was once widely used in agriculture for its excellent broad-spectrum insecticidal effect. However, due to its high toxicity, long half-life, and difficult degradation properties, it poses a serious challenge to water environment pollution. In this study, an electrode-free discharge microwave-induced plasma technology was used to rapidly and efficiently degrade methomyl in aqueous solution. In this experiment, the statistical design of experiments (DOE) was adopted to optimize the plasma degradation parameters. Under the optimized parameters ( P = 140 W, D = 0 mm, R = 0.5 L/min), 78.4% removal of 50 mg/L of methomyl was achieved after 8 min. The optical emission spectrometry and free radical detection experiments showed that the active substances generated by the collision reaction between plasma and water molecules occurring at the gas–liquid interface were the key factors to exert the degradation effect. The degradation rate of methomyl decreased by 73.2% after the addition of tert-butanol (OH burster), while it decreased by only about 12.0% after the addition of peroxidase. These implied that ∙OH was largely responsible for methomyl degradation. In addition, based on the detected intermediates, possible degradation mechanisms and pathways were analyzed.
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Electrocatalysis
·
2023
·
IF 3.1
Wei Guobing,Fan Qiqi,Hong Nian,Cui Hanfeng,Zhang Wenxing,Rustam Mijit,Alim Alxir,Jiang Tao,Dong Huanhuan,Fan Hao
Here, we developed a novel reagentless aptamer sensor for the detection of aflatoxin B1 (AFB1) that uses a DNA walker strategy and bisferrocene to enhance the signal. First, the special binding of AFB1 and AFB1 aptamer activates the DNAzyme strand. Then, with the aid of Mn 2+ , the DNAzyme strand sequentially cuts the bisferrocene immobilized substrate strand, resulting in a change in the electrochemical signal. And we wrapped Mn 2+ in the organometallic framework UiO-66(Zr)-(COOH) 2 and integrated it with DNA walker. It enables the initiation of the cut reaction without the addition of Mn 2+ during the detection, enabling real-time monitoring. Based on the above advantages, under optimal conditions, the sensor shows good detection linearity in the range of 0.1 pg/mL to 1000 ng/mL, and the detection limit is 4.81 fg/mL. The sensor has also been successfully applied for the detection of AFB1 in complex matrices, including peanut oil samples, with high sensitivity and reagentless operation. In addition, the proposed aptamer sensor can be used to detect other mycotoxins by replacing the aptamer, thus providing an efficient platform for the detection and real-time monitoring applications. Graphical Abstract
A reagentless electrochemical aptamer sensor for AFB1 detection was constructed based on DNAzyme-driven DNA walker and bisferrocene. No significant change in electrochemical signal was observed when the target AFB1 was not added. The electrochemical signal was significantly reduced when the target AFB1 was added.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 13
·6038-6054
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IF 4.5
Xin Lei,Li Pengchang,Li Hao,Sun Weixiang,Zhang Chen,Zhang Kai,Yin Xiaoli,Yu Sirong
Metals with anti-icing and anti-corrosion properties are necessary in marine fields. Here, a robust decoupling superhydrophobic coating with anti-icing and anti-corrosion performance was fabricated by sequential spraying unmodified micron-sized SiO 2 particles and modified nano-sized TiO 2 particles. The unmodified micron-sized SiO 2 particles with sturdiness act as a protective framework for the modified nano-sized TiO 2 particles with superhydrophobicity, which effectively enhance the robustness of coatings. Consequently, the decoupling coating reveals the excellent superhydrophobicity (contact angle > 157°, roll off angle < 5°) and promising robustness (the friction coefficient of ~ 0.25). In addition, the superhydrophobic property endows the coating with admirable anti-icing performance, in which the icing time of decoupling coating is about 10 times of the substrate. The synergistic effect of epoxy resin adhesive layer and superhydrophobicity enables the decoupling coating exhibit excellent anti-corrosion property in electrochemical measurements and immersion tests. Therefore, the decoupling superhydrophobic coating with significant performance advantages could expand the application fields of other various solids.
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Human Cell
·
2023
·
IF 4.3
Hu Lianying,Cheng Zhen,Wu Lunan,Luo Liangliang,Pan Ping,Li Shujin,Jia Qiyu,Yang Ning,Xu Bin
Osteogenic differentiation plays important roles in the pathogenesis of osteoporosis. In this study, we explored the regulatory mechanism of histone methyltransferase SET domain bifurcated 1 (SETDB1) underlying the osteogenic differentiation in osteoporosis. The common osteoporosis-related genes were retrieved from the GeneCards, CTD, and Phenolyzer databases. The enrichment analysis was conducted on the candidate osteoporosis-related genes using the PANTHER software, and the binding site between transcription factors and target genes predicted by hTFtarget. The bioinformatics analyses suggested 6 osteoporosis-related chromatin/chromatin binding protein or regulatory proteins (HDAC4, SIRT1, SETDB1, MECP2, CHD7, and DKC1). Normal and osteoporosis tissues were collected from osteoporosis patients to examine the expression of SETDB1. It was found that SETDB1 was poorly expressed in osteoporotic femoral tissues, indicating that SETDB1 might be involved in the development of osteoporosis. We induced SETDB1 overexpression/knockdown, orthodenticle homeobox 2 (OTX2) overexpression, activation of Wnt/β-catenin or BMP-Smad pathways alone or in combination in osteoblasts or ovariectomized mice. The data indicated that SETDB1 methylation regulated H3K9me3 in the OTX2 promoter region and inhibited the expression of OTX2. Besides, the BMP-Smad and Wnt/β-catenin pathways were inhibited by OTX2, thereby resulting in inhibited osteogenic differentiation. Animal experiments showed that overexpressed SETDB1 could promote the increase of calcium level and differentiation of femoral tissues. In conclusion, upregulation of SETDB1 promotes osteogenic differentiation by inhibiting OTX2 and activating the BMP-Smad and Wnt/β-catenin pathways in osteoporosis.
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