JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
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IF 8.128
Wei Chen,Zhiyang Guo,Hao Yu,Qingyun Liu,Min Fu
Glycoproteins play vital roles in living organisms and often serve as biomarkers for some disease. However, due to the low content of glycoprotein in biological fluids, selective detection of glycoproteins is still a challenging issue that needs to be addressed. In this study, molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under physiological pH was proposed. Monodispersed glycoprotein imprinted particles (SiO 2 @PEI/MIPs) was first prepared based on surface imprinting strategy using horseradish peroxidase (HRP) as template, and polyethyleneimine (PEI) was used to increase the number of boronic acid groups. The binding experiment indicated that the SiO 2 @PEI/MIPs hold satisfactory adsorption capacity (1.41 μmol/g), rapid adsorption rate (40 min) and preferable selectivity toward HRP. Then the SiO 2 @PEI/MIPs was assembled into close-packed colloidal array to construct a label free optical sensor (denoted as GICA). Benefiting from the high ordered photonic crystal structure, binding of HRP onto the GICA could be directly readout from the changes in structure color and diffracted wavelength. The structure color of the GICA changed from bright blue to yellow with the diffraction wavelength red shifted 59 nm when the HRP concentration increased from 2.5 to 15 μmol/L. Importantly, the GICA was capable of detecting HRP from human serum samples. All those results indicated the potential of the GICA for naked-eye detection of glycoprotein.
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RSC Advances
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2021
·Vol. 11
·期 46
·29042-29051
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IF 3.361
Fen Zhang,Yanling Niu,Yantao Li,Qian Yao,Xiaoqi Chen,Haijun Zhou,Mengmeng Zhou,Jijun Xiao
Smart polymeric vesicles with both tertiary amine and epoxy functional groups were fabricated for the first time via a reversible addition–fragmentation chain transfer dispersion polymerization approach, using (2-(diisopropylamino)ethyl methacrylate (DIPEMA) and glycidyl methacrylate (GlyMA) in an ethanol–water mixture. Monitoring of the in situ polymerization revealed the low molecular weight distributions and the intermediate structures of spheres and worms, indicating an evolution in particle morphology. A phase diagram was constructed for reproducible fabrication of the vesicles, and copolymer composition was found to be more related to particle morphology. The vesicles exhibited superior structural stability for the cross-linking of the core through epoxydiamine chemistry, and intelligent pH responsibility due to the presence of the tertiary amine groups. The cross-linked vesicles showed good stability and reversibility during the swelling and shrinking cycles by switching the pH values, which endowed them with potential cell-like transmission functions. This research thus provides a method for producing structurally stable pH-responsive polymeric vesicles, and the reported vesicles are based on commercially available starting materials for possible industrial scale-up.
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International Journal of Nanomedicine
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2021
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IF 6.4
Ronghua Tan,Danlei Tian,Jiaoyan Liu,Congcong Wang,Ying Wan
Background Chemotherapeutic drugs used for tumor treatments often show limited efficiency due to their short lifetime, nonspecific delivery, and slow or insufficient intracellular drug release, and also, they can cause severe system or organ toxicity. The development of chemotherapeutic nanomedicines with high efficacy and satisfactory safety still remains a challenge for current tumor chemotherapy.Methods A novel type of conjugate was synthesized using hydroxyethyl starch (HES) as a carrier while binding doxorubicin (DOX) onto HES backbone through a pH/redox responsive linker containing both disulfide and hydrazone bonds in series. The built conjugates were self-assembled into nanoparticles (NPs) (HES-SS-hyd-DOX NPs) for achieving enhanced antitumor therapy and adequate safety.Results HES-SS-hyd-DOX NPs had a certain ability for the tumor-orientated drug accumulation and were capable of releasing DOX itself rather than DOX derivatives. It was found that the pH/redox responsive linkage enabled the NPs to achieve fast and sufficient intracellular drug release. Based on the tumor-bearing mouse model, antitumor results demonstrated that these NPs were able to inhibit the growth of the advanced tumors with significantly enhanced efficacy when compared to free DOX, and to those conjugate NPs containing only a single responsive or unresponsive bond. Besides, HES-SS-hyd-DOX NPs also showed adequate safety to the normal organs of the treated mice.Conclusion The pH/redox responsive linkage in HES-SS-hyd-DOX was found to play a critical role in mediating the drug accumulation and the fast and sufficient intracellular drug release. The HES-exposed surface of HES-SS-hyd-DOX NPs endowed the NPs with long circulation capability and remarkably reduced the DOX-induced side effects.
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OPTICAL MATERIALS
·
2019
·
IF 2.687
Penghui Chen,Qiang Liu,Xiaoying Li,Yagang Feng,Xiaopu Chen,Xin Liu,Lexiang Wu,Jiang Li
Y 0.16 Zr 0.84 O 1.92 nanopowders were co-precipitated from the mixed solution of zirconium oxychloride and yttrium nitrate using ammonia as the precipitant. The influences of terminal pH value on the chemical composition and morphology of the Y 0.16 Zr 0.84 O 1.92 precursors and powders were characterized. The precursors with terminal pH = 8.5–9.25 completely transform to cubic ZrO 2 phase when calcined at 800 °C. The Y 0.16 Zr 0.84 O 1.92 powder with terminal pH = 9.0 shows the best dispersity, which is beneficial to the fabrication of transparent ceramics. Using the synthesized nanopowders as starting materials, Y 0.16 Zr 0.84 O 1.92 ceramics were prepared by pre-sintering at 1350 °C for 2 h in air and HIP post-treatment at 1800 °C for 3 h under 200 MPa Ar without sintering additives. The Y 0.16 Zr 0.84 O 1.92 ceramic sample fabricated from the powder with terminal pH = 9.0 shows the best optical quality, and its in-line transmittance exceeds 56.4% in the region of 400–850 nm, reaching 67.8% at 800 nm.
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NANO
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2019
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IF 1.293
Ben Liu,Qiusheng Song,Qiancheng Zhang,Haihong Ma,Ziwen Zhang,Dapeng Wang
In this study, a kind of novel rare-earth nanocrystals/hydroxypropylcellulose–poly (acrylic acid) (HPC–PAA) complex fluorescent nanogel, its responsive behavior to environmental temperature and pH value were reported. For preparation, with HPC being used as template, HPC–PAA nanogel was first synthesized by polymerization and crosslinking reaction of acrylic acid (AA) in water solution. In the process, a redox initiator, which composed of ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED), was used to initiate the reaction. Then, the as-prepared nanogel reacted with Ce(NO 3 ) 3 and NH 4 F solution successively, and a novel CeF 3 nanocrystals/HPC–PAA complex nanogel was fabricated. The microstructure of the nanogel was characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), Thermogravimetric (TG) and Transmission electron microscopy (TEM). The environmental sensitivity of the nanogel was investigated by photoluminescence (PL) and UV–Visible spectrophotometer (UV–Vis). The thermo- and pH-sensitive fluorescence were studied by PL at various temperatures and pH values; besides, the behavior of drug loading and release was researched by PL with a famous antibiotic of Ibuprofen as model drug. The results show that the PL intensity of the nanogel was largely affected by environmental temperature, or content of Ibuprofen loaded in the nanogel. The as-prepared nanogel can be used as useful sensitive material to detect temperature and pH value change, and drug loading or release property of Ibuprofen can be detected by PL emission of the nanogels conveniently.
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Polymers
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2019
·Vol. 11
·期 10
·1564
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IF 3.164
Tong Wang,Xuan Zhang,Zichao Wang,Xiuzhong Zhu,Jie Liu,Xin Min,Tao Cao,Xiaodong Fan
Stimuli-responsive conductive hydrogels have a wide range of applications due to their intelligent sensing of external environmental changes, which are important for smart switches, soft robotics, and flexible sensors. However, designing stimuli-responsive conductive hydrogels with logical operation, such as smart switches, remains a challenge. In this study, we synthesized pH-responsive conductive hydrogels, based on the copolymer network of acrylic acid and hydroxyethyl acrylate doped with graphene oxide. Using the good flexibility and conductivity of these hydrogels, we prepared a flexible sensor that can realize the intelligent analysis of human body motion signals. Moreover, the pH-responsive conductive hydrogels were integrated with temperature-responsive conductive hydrogels to develop logic gates with sensing, analysis, and driving functions, which realized the intellectualization of conductive hydrogels.Keywords:stimuli-responsive conductive hydrogels;pH-responsive;composite hydrogels;flexible sensors;logic gates
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PROCESS BIOCHEMISTRY
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2019
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IF 2.883
Shan Bai,JunFen Wan,XueJun Cao
Aqueous two-phase systems (ATPS) are a promising biotechnology in bioseparation. However, recycling of the phase-forming polymer is a key issue. In this research, new recyclable ATPS composed of two pH-responsive polymers (P ADB4.01 and P MDBH3.27 ) were prepared. The ATPS could be recycled by adjusting the pH of the solution, with recovery of 93.8%. The ATPS were evaluated for the first time in the partitioning of tylosin. The effects of the main parameters, such as the polymer concentration, pH, temperature, salt species and concentrations, on the partition of crude tylosin and tylosin from fermentation broth were studied. In the 2% P MDBH3.27 /3% P ADB4.01 ATPS, the partition coefficient (K) and extraction recovery (ER top ) of crude tylosin could reach 5.12 and 93.9%, respectively, in the presence of 90 mM KCl at pH 5.83 and 25 °C. In the 2.5% P MDBH3.27 /3% P ADB4.01 ATPS, the K and ER top of tylosin from fermentation broth reached 3.58 and 87.3%, respectively, in the presence of 20 mM KBr at pH 5.69 and 25 °C. Through partition mechanism analysis by Fourier transform infrared spectroscopy (FT-IR) and interphase potential measurements, the appearance of a new absorption peak and interphase potential difference, respectively, may contribute to the partitioning of tylosin in the P ADB4.01 -enriched top phase.
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CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Yongbin Xu,Yaxin Zi,Jinfeng Lei,Xingyue Mo,Zilun Shao,Yuyao Wu,Ye Tian,Defu Li,Changdao Mu
Various nanoparticles have been developed for tumor-targeted drug delivery. However, nanoparticles with effective targeting and intelligent release capacity are still deficient. Herein, we present new pH-responsive and neutral charged nanoparticles for tumor-targeted anticancer drug delivery. Oxidized starch was synthesized and simultaneously modified by cholesterol and imidazole to obtain amphiphilic cholesterol/imidazole modified oxidized-starch (Cho-Imi-OS). Cho-Imi-OS easily self-assembled into nanoparticles by dialysis. Curcumin was selected as model drug to be encapsulated into the hydrophobic core of nanoparticles. The results showed that curcumin would effectively accumulate in cancer cells by encapsulating into the nanoparticles owing to the nano-sized structure and near neutral charged property of nanoparticles. Curcumin was released faster at pH 5.5 than that at pH 7.4 from the curcumin-loaded nanoparticles (Cur-NPs), indicating the pH-triggered release capacity of Cur-NPs after endocytosis by endosomes since the pH is low to 5.0∼6.0 in endosomes. Naturally, Cur-NPs showed significantly strong inhibitory effect on cancer cells.
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FEBS Journal
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2019
·Vol. 287
·期 9
·1816-1829
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IF 4.739
Man Zhang,Xiaoyao Ma,Honglei Xu,Wenbo Wu,Xin He,Xiaoying Wang,Min Jiang,Yuanyuan Hou,Gang Bai
Swertiamarin (SW), a representative component in Flos Lonicerae Japonicae , has been reported to exert significant activity in preventing infections. In this research, we aim to clarify the details of SW and its target to explore SW's underlying anti-inflammatory mechanisms. An azide labeled SW probe was synthesized for protein target fishing, and the results demonstrated that AKT could be captured specifically. Immunofluorescence colocalization with AKT was implemented by a click reaction of the SW probe and alkynyl CY5. The result showed that AKT was one of the targets of SW. Then, a competitive combination experiment using a set of AKT inhibitors and a membrane translocation experiment confirmed that SW might target the pleckstrin homology (PH) domain of AKT. This specific binding directly deactivated the phosphorylation of AKT on both Ser473 and Thr308, which induced the dephosphorylation of IKK and NF-κB. Finally, proinflammatory cytokines (TNF-α, IL-6, and IL-8) were suppressed both in cells and in acute lung injury animal model by targeting AKT-PH domain. This study demonstrated that SW functions as a natural AKT inhibitor and presents significant anti-inflammatory activity by directly regulating the AKT-PH domain and inhibiting downstream inflammatory molecules.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
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IF 3.973
Hu Lin,Quan Wang,Rui Zhong,Zhen Li,Weifeng Zhao,Yuanwei Chen,Meng Tian,Xianglin Luo
pH-responsive nanocarriers such as polymeric micelles that self-assemble from amphiphilic copolymers containing amino groups have been limited by their significant effects on the blood and thus compromise of their hemocompatibility due to the amino group-induced positive charges. Here we report a biomimetic phosphorylcholine strategy to improve the hemocompatibility of the pH-responsive micelles with positive charges. Amphiphilic copolymers containing different number of tertiary amino groups were synthesized in five steps through ring opening polymerization, azide reaction, thio-bromo “Click” chemistry, and atom transfer radical polymerization to self-assemble biomimetic phosphorylcholine micelles with pH-responsiveness, which shown no significant effects on red blood cells, coagulation, and platelet activation. Moreover, albumin adsorption on the micelles was significantly lower than that of polycaprolactone-methoxypolyethylene glycol (PCL-mPEG) control, and in terms of immune cells, the micelles showed controllable phagocytosis that dependent on the number of tertiary amino groups, in which the one containing four tertiary amino groups in its corresponding copolymer remains had a lower phagocytosis by whole blood leukocyte than that of PCL-mPEG. Based on these results, the hemocompatibility related mechanism of the micelles was discussed and proposed. Our findings demonstrated that this biomimetic phosphorylcholine is a promising strategy to improve the hemocompatibility of the positively charged nanocarriers.
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Nano Research
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2019
·Vol. 12
·期 11
·2815-2826
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IF 8.515
Zhang Xiaolei,Zhang Chuangnian,Cheng Mingbo,Zhang Yahui,Wang Wei,Yuan Zhi
The strategy of pH-responsive aggregation in tumor micro-environment (TME) provides an intriguing platform for enhancing tumor retention and exerting therapeutic effects sufficiently. In this work, we have designed an intelligent dual pH-responsive self-aggregating nano gold system (Au@PAH-Pt/DMMA) for the combined chemo-radiotherapy, in which a “charge-reversal like” strategy was utilized to realize irreversible stable aggregation and pH-specific release of cisplatin prodrug in TME. Responsive aggregation increases the cellular uptake of Au@PAH-Pt/DMMA by 55%–60%, and the cellular uptake of Pt after X-ray irradiation can be further enhanced by 80%. Additionally, responsive aggregation greatly slows down the rate of efflux from tumor in vivo . This system not only promotes B16 cell apoptosis as a chemotherapeutic agent (30.4%), it also enhances the effect of chemo-radiotheray (CRT) by promoting apoptosis as a radiosensitizer (55.3%). The colony formation assay results were fitted to cell survival curve of B16 cells and the sensitization enhancement ratio (SER) was calculated to be 1.29, which shows a good radiosensitizing ability. When exposed to X-ray, this nanoplatform reached the ideal therapeutic effect, and the tumor inhibition rate of Au@PAH-Pt/DMMA reached 91.6% with low drug administration frequency and dose of X-ray. Overall, the dual pH-responsive nanoparticles Au@PAH-Pt/DMMA could effectively enhance tumor therapeutic efficiency by combined chemo-radiotherapy, which provides a potential method for clinical transformation of cancer treatment.
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Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 6
·1223-1234
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IF 5.047
Huiru Li,Xinyue Zhang,Xilin Lin,Shuqiang Zhuang,Yan Wu,Zhong Liu,Jianhua Rong,Jianhao Zhao
Based on the concept of starving tumor therapy, in this study we put forward a new idea that the pH-sensitive Ca2+ delivery of calcium carbonate nanoparticles (CaCO3 NPs) induced blood coagulation of tumor vessels, and first explored the effect of CaCO3 NPs on the in vitro and in vivo blood coagulation by acid stimulus. CaCO3 NPs with a size of about 100 nm and a porous structure of several nanometers were synthesized in an emulsion system, which showed a high loading capacity (49%) of doxorubicin hydrochloride (DOX) with an encapsulation efficiency of 98% and a pH-sensitive drug delivery. The hemolysis test showed that CaCO3 NPs were blood compatible. The in vitro Ca2+ delivery and blood clotting tests indicated that CaCO3 NPs pH-sensitively released Ca2+, and caused rapid blood coagulation at pH 5.0 but no thrombus at pH 7.4. Confocal laser scanning microscopy showed that after uptake by MCF-7 or MDA-MB-231 breast cancer cells, CaCO3 NPs mainly distributed in endosomes/lysosomes within the initial 2 h and then decomposed by acid stimulus, leading to the intracellular delivery of Ca2+ that subsequently migrated outside the cells. CaCO3 NPs were nontoxic to NIH3T3 mouse fibroblasts, but highly toxic to both MCF-7 and MDA-MB-231 cells after loading DOX. After topical administration into the breast tumors of mice, CaCO3 NPs evoked significant thrombosis and hemorrhage of tumor vasculature by hematoxylin–eosin and Masson's trichrome staining. These results indicated that CaCO3 NPs could induce blood coagulation via acid stimulus, showing potential applications in blocking tumor vessels for starving tumor therapy.
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