EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Sihan Shen,Ran Xie,Yiqun Wang,Lingyu Jia,Zhangran Zhu,Hai Ma,Lifang Wang,Feng Sui,Yu Zhao,Qinghe Zhao,Yanjun Chen
Ferroptosis represents a novel procedure of programmed cell death driven by iron-dependent lipid peroxidation, and may offer a promising strategy for cancer therapy. In this study, we developed poly (2-ethyl-2-oxazoline)-poly (lactic acid)-SS-poly (β-amino ester) (PEOz-PLA-SS-PBAE) loaded with β-elemene. The disulfide bond mediated depletion of glutathione (GSH) and inactivation of glutathione peroxidase 4 (GPX4) will lead to inactivation of the antioxidant system of the tumor cell, and β-elemene has the capacity to generate reactive oxygen species (ROS), and the above changes would induce a disruption of the redox balance within the tumor cell, resulting in ferroptosis of the tumor cell. In conclusion, in this study, water insoluble β-elemene was solubilized by the triblock copolymer to construct a pH/GSH dual-responsive nano-delivery system, and combined with iron death and chemotherapy to exert combinatory inhibition effect on breast cancer cells, which provides a new idea for the potential clinical application of β-elemene
✓ 已验证
产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xuedi Zhang,Yue Zhang,Kai Xu,Jianhua Qin,Dehe Wang,Lijun Xu,Chuanwen Wang
ABSTRACT Dermanyssus gallinae is a major hematophagous ectoparasite in layer hens. Although the acaricide β-cypermethrin has been used to control mites worldwide, D. gallinae has developed resistance to this compound. Carboxylesterases ( CarEs ) are important detoxification enzymes that confer resistance to β-cypermethrin in arthropods. However, CarEs associated with β-cypermethrin resistance in D. gallinae have not yet been functionally characterized. Here, we isolated a CarE gene ( Deg-CarE ) from D. gallinae and assayed its activity. The results revealed significantly higher expression of Deg-CarE in the β-cypermethrin-resistant strain ( RS ) than in the susceptible strain ( SS ) toward α-naphthyl acetate ( α-NA ) and β-naphthyl acetate ( β-NA ). These findings suggest that enhanced esterase activities might have contributed to β-cypermethrin resistance in D. gallinae . Quantitative real-time PCR analysis revealed that Deg-CarE expression levels were significantly higher in adults than in other life stages. Although Deg-CarE was upregulated in the RS, significant differences in gene copy numbers were not observed. Additionally, Deg-CarE expression was significantly induced by β-cypermethrin in both the SS and RS. Moreover, silencing Deg-CarE via RNA interference decreased the enzyme activity and increased the susceptibility of the RS to β-cypermethrin, confirming that Deg-CarE is crucial for β-cypermethrin detoxification. Finally, recombinant Deg-CarE ( rDeg-CarE ) expressed in Escherichia coli displayed high enzymatic activity toward α/β-NA. However, metabolic analysis indicated that rDeg-CarE did not directly metabolize β-cypermethrin. The collective findings indicate that D. gallinae resistance to β-cypermethrin is associated with elevated CarEs protein activity and increased Deg-CarE expression levels. These findings provide insights into the metabolic resistance of D. gallinae and offer scientific guidance for the management and control of D. gallinae .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Sustainable Systems
·
2025
·
IF 6.1
Wen-Jin An,Xiao-Yong Yu,Chu-Han Liu,Yuan-Li Leng,Yong Chen,Yu Liu
Supramolecular hydrogels have significant research implications for the environmental application of dyes and bisphenols wastewater. Herein, a magnetic Fe 3 O 4 -P(AAm-AMPS-CDA) supramolecular hydrogel is reported, which is constructed by acrylamide, 2-acrylamido-2-methylpropane sulfonic acid, acrylated β-cyclodextrin, and Fe 3 O 4 nanoparticles via thermal polymerization, exhibiting good adsorption properties for cationic dyes and bisphenols. UV–vis spectra show that the adsorption efficiencies of the hydrogel reach 96% for crystal violet and 98% for methylene blue. Meanwhile, β-CDA cavities can form an inclusion complex with bisphenols, achieving adsorption efficiencies of 88% for bisphenol A and 69% for bisphenol F, with no significant decrease in adsorption rates even after seven cycles. The adsorption kinetic experiments indicate that the adsorption process follows the pseudo-second-order kinetics model, and the Weber-Morris intraparticle diffusion model reveals the diffusion mechanism. Additionally, in the presence of NaCl and humic acid, the hydrogel still maintains a high adsorption rate, and there is no significant decline in adsorption across different environmental water qualities, indicating that the hydrogel has great application potential. Importantly, the hydrogel exhibits ferromagnetic characteristics, allowing for separation with a magnet after adsorption is complete and facilitating rapid adsorption and separation of pollutants in wastewater.
✓ 已验证
产品关联
DOI
高影响因子
Materials Research Letters
·
2024
·
IF 8.6
Xiaoqi Mao,Lianwen Wang,Guodong Sun,Xuezhe Zhang,Yongqing Fu,Longlong Dong
Breaking trade-off barriers of strength-ductility in Ti alloys and their composites has been a key challenge for their engineering applications. Herein, we developed a new strategy of introducing β-Ti ductile zones in heterostructured (Cu@CNTs/TC4)+TC18 composites, which were synthesized using two-stepped ball milling, spark plasma sintering and hot rolling processes. Such composites are composed of TC18-coarse grain zones (CGZ) and (TiC+α'')-reinforced TC4-fine grain zones (FGZ). They achieve an exceptional high strength and high ductility, which are superior to those of TC4 alloys and homogeneous Cu@CNTs/TC4 composites. Heterogeneous interfacial microstructures of TC4-FGZ/TC18-CGZ play an important role in strengthening and toughening effects. The grain-coordinated deformation between TC18-CG and TC4-FG is also the key reason for achieving an exceptional ductility.
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Guang-Xin Mao,Jia-Min Wu,Chong Tian,Chun-Lei Liu,Xin Lin,Fen Wang,Hai-Sheng Xu,Yu-Sheng Shi
Porous Si 3 N 4 ceramics are widely applied in aerospace and mechanical fields owing to their excellent properties. Furthermore, vat photopolymerization (VPP) technology can fabricate Si 3 N 4 components with complicated structures and high precision, but its layer-by-layer printing method leads to poor mechanical properties of ceramics. In this study, porous Si 3 N 4 ceramics with a porosity of 28.41 % strengthened by directional β-Si 3 N 4 were fabricated by combining VPP technology and seeding method. Rheological behavior and curing properties of the slurry were explored, and the influence of β-Si 3 N 4 content on the mechanical properties of printed Si 3 N 4 ceramics was investigated systematically. With the increase of β-Si 3 N 4 content, the orientation degree of β-Si 3 N 4 grains increased gradually, while fracture toughness and flexural strength of the ceramics exhibited a trend of increased first and then decreased and Vickers hardness gradually decreased. As β-Si 3 N 4 content increased to 5 wt%, the fracture toughness and flexural strength of porous Si 3 N 4 ceramics were improved from 4.23 MPa m 1/2 and 214.7 MPa–5.65 MPa m 1/2 and 272.0 MPa, respectively. Therefore, this work indicates that vat photopolymerization combined with seeding method is a promising approach for the fabrication of porous Si 3 N 4 ceramics with high performance and complex structures.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 11
·8346-8353
·
IF 4.6
Qingshan Rong,Wei Ding,Guogang Liu,Xiangyu Fu,Yan Shi,Zhiqiang Zhang,Zhiwei Yao
In this study, we found that β-Mo2C prepared at different carbonization temperatures exhibited significantly different catalytic activities for the reverse water gas shift (RWGS) reaction. The β-Mo2C synthesized at 600 °C demonstrated notably higher activity compared to those prepared at 700 °C and 800 °C. This enhanced activity was likely attributed to its improved redox properties, which were primarily driven by a smaller crystallite size and the presence of MoxOCy species. Therefore, we proposed that the crystallite size and MoxOCy content were the key factors governing the RWGS activity of β-Mo2C. Clearly, both factors were strongly influenced by the carbonization temperature. Notably, the β-Mo2C prepared at 600 °C even outperformed Cu-doped β-Mo2C prepared at 700 °C under similar reaction conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2024
·Vol. 2024
·期 1
·7645677
·
IF 3.5
Fan Yang,Lu Sun,Yingjie Gao,Jingzhen Liang,Wenqian Ye,Wenjing Yang,Siyi Xie,Jiangtao Zhou,Rongshan Li
Background: Renal involvement resulting from hyperuricemia, known as hyperuricemia nephropathy (HN), is characterized by chronic tubulointerstitial inflammation caused by extensive urate crystal deposition. Managing this condition requires straightforward preventive or therapeutic interventions, primarily through dietary measures. Methods: In this study, the mouse model of HN was established using yeast extract combined with potassium oxonate. The effect and potential mechanism of β-sitosterol in treating HN were investigated through biochemical indexes, pathological changes, untargeted metabolomics, and network pharmacology. Results: β-Sitosterol reduced the levels of four biomarkers of HN: uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), and xanthine oxidase (XOD). It also mitigated inflammatory injury in renal tissues and reversed the abnormal expression of four key urate transporter proteins: glucose transporter protein 9 (GLUT9), organic anion transporter 1 (OAT1), ATP-binding cassette transporter G2 (ABCG2), and urate transporter 1 (URAT1). To explore the mechanism of β-sitosterol in treating HN, this study employed network pharmacology and metabolomics to analyze 27 intersecting gene targets and 14 differential metabolites. The findings indicated that glutathione (GSH) metabolism might be a crucial pathway. Treatment with β-sitosterol increased the levels of reduced GSH as well as the activity and expression of 6-phosphogluconate dehydrogenase (G6PDH) in mice, thereby effectively modulating GSH metabolism. This study proposes a novel strategy using β-sitosterol for treating HN, providing a promising approach for addressing this condition.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Meiqi Xu,Junwei Liu,Jianming Yu,Jingwen Wang,Hui Li,Ting Zhong,Yanli Hao,Zhuoyue Li,Jingru Wang,Xu Huang,Hui Wang,Yubo Tian,Heng Zhao,Qingchao Wei,Xuan Zhang
Improving nanomedicine uptake by tumor cells is key to achieving intracellular drug delivery. In this study, we found that methyl-β-cyclodextrin (MβCD) can significantly promote the intracellular accumulation of nanoparticulated α-linolenic acid-paclitaxel conjugates (ALA-PTX NPs) via enhanced clathrin-mediated endocytosis and limited degradation in lysosomes. Our in vitro results indicated that MβCD not only reduced the plasma membrane cholesterol content and increased plasma membrane fluidity, leading to ALA-PTX NPs being more easily incorporated into the plasma membrane, further enhancing membrane fluidity and making the plasma membrane more susceptible to tensile deformation, forming intracellular vesicles to enhance ALA-PTX NP cellular uptake, but also destroyed lysosomes and then limited ALA-PTX NPs’ degradation in lysosomes. In HepG2 tumor-bearing mice, MβCD was also able to enhance the antitumor activity of ALA-PTX NPs in vivo. Moreover, we found that MβCD specifically promoted PUFA-paclitaxel conjugate NP cellular uptake. The cellular uptake of PTX liposome which shares an endocytosis pathway with ALA-PTX NPs could be enhanced by MβCD combined with ALA or ALA-PTX NPs. Therefore, we suggested that MβCD combined with polyunsaturated fatty acid-conjugation would be an effective strategy for improving intracellular delivery of nanoparticulated chemotherapeutic drugs used for combination administration to enhance antitumor efficiency.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomolecules
·
2024
·Vol. 14
·期 12
·1553
·
IF 4.8
Zifu Ni,Linshang Zhang,Azhen Nie,Huan Wang,Xiaoling Wu
As the only naturally occurring β-amino acid, β-alanine has important application prospects in many fields. Driven by the huge demand, biosynthesis is becoming more and more popular as a potential alternative to the chemical synthesis of β-alanine. Although the direct pathway from L-aspartic acid to β-alanine, catalyzed by L-aspartic acid-α-decarboxylase (PanD), is ideal for β-alanine synthesis, it is hindered by the high cost of the substrate and limited economic viability. In this work, a cell-free dual enzyme cascade system based on methylaspartate lyase (EcMAL) and panD was constructed to safely and efficiently synthesize β-alanine using fumarate as a substrate. Taking the previously engineered EcMAL as the target, CgPanD was finally screened as the best candidate through gene mining, sequence alignment, and enzyme property analysis. Finally, under the optimal conditions of 35 °C, pH 8.0, and EcMAL: CgPanD concentration ratio of 1:5, the yield of β-alanine reached 80% theoretical yield within 120 min. This study provides a potential strategy for the biosynthesis of β-alanine, paving the way for future industrial-scale production.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Qi Chen,Ziwei Huang,Jing Chen,Xiaoyu Tian,Rong Zhang,Qi Liang,Zhongqiu Liu,Yuanyuan Cheng
β2 adrenergic receptor (β2AR) is a G-protein-coupled receptor involved in cardiac protection. In chronic heart failure (CHF), persistent sympathetic nervous system activation occurs, resulting in prolonged β2AR activation and subsequent receptor desensitization and downregulation. Notoginsenoside R1 (NGR1) has the functions of enhancing myocardial energy metabolism and mitigating myocardial fibrosis. The mechanisms of NGR1 against ischemic heart failure are unclear. A left anterior descending (LAD) artery ligation procedure was performed on C57BL/6 J mice for four weeks. From the 4th week onwards, they were treated with various doses (3, 10, 30 mg/kg/day) of NGR1. Subsequently, the impacts of NGR1 on ischemic heart failure were evaluated by assessing cardiac function, morphological changes in cardiac tissue, and the expression of atrial natriuretic peptide (ANP) and beta-myosin heavy chain (β-MHC). H9c2 cells were protected by NGR1 when exposed to OGD/R conditions. H9c2 cells were likewise protected from OGD/R damage by NGR1. Furthermore, NGR1 increased β2AR levels and decreased β2AR ubiquitination. Mechanistic studies revealed that NGR1 enhanced MDM2 protein stability and increased the expression of MDM2 and β-arrestin2 while inhibiting their interaction. Additionally, under conditions produced by OGD/R, the protective benefits of NGR1 on H9c2 cells were attenuated upon administration of the MDM2 inhibitor SP141. According to these findings, NGR1 impedes the interplay between β-arrestin2 and MDM2, thereby preventing the ubiquitination and degradation of β2AR to improve CHF.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
·
2024
·
IF 2.3
Zhihao Zhang,Dongliang Zhang,Kai Su,Dongqiang Wu,Qiqi Hu,Tianying Jin,Tingting Ye,Rongrong Zhang
Background Lung adenocarcinoma (LUAD) patients are implicated in poor prognoses and increased mortality rates. Metastasis, as a leading cause of LUAD-related deaths, requires further investigation. Highly metastatic cancer cells often exhibit extensive characteristics of epithelial-mesenchymal transition (EMT). This study attempted to identify novel targets associated with LUAD metastasis and validate their specific molecular mechanisms. Methods Bioinformatics was conducted to determine NTSR1 expression in LUAD and the enriched pathways. Immunohistochemical analysis was used to assess NTSR1 expression in LUAD tissue. qRT-PCR examined expressions of NTSR1 and Wnt/β-Catenin pathway-related genes in LUAD cells. Transwell assayed cell migration and invasion. Cell adhesion experiments were conducted to evaluate cell adhesion capacity. Western blot analysis was employed to examine expression of EMT, Wnt/β-Catenin pathway, and cell adhesion markers. Results NTSR1 was upregulated in LUAD tissues and cells, and enriched in EMT pathway. Knockdown of NTSR1 reduced migration, invasion, and adhesion abilities in LUAD cells, and inhibited EMT progression and Wnt/β-Catenin pathway. Rescue experiments demonstrated that β-Catenin activator SKL2001 reversed repressive influence of NTSR1 knockdown on LUAD cell malignant phenotypes and EMT progression. Conclusion The data obtained in this study suggested that NTSR1 stimulated EMT and metastasis in LUAD via Wnt/β-Catenin pathway. This finding may provide options for overcoming LUAD metastasis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Liao Zhengzheng,Hu Jinfang,Li Zhentao
CD-MONs (β-cyclodextrin-based microporous organic networks), derived from β-cyclodextrin, possess notable hydrophobic characteristics, a considerable specific surface area, and remarkable stability, rendering them highly advantageous in separation science. This research aimed to investigate the utility of CD-MONs in chromatography separation. Through a monomer-mediated technique, we fabricated an innovative CD-MON modified capillary column for application in open-tubular capillary electrochromatography (OT-CEC). The CD-MON-based stationary phase on the capillary’s inner surface was analyzed using Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). We assessed the performance of the CD-MON modified capillary column for separation purposes. The microstructure and pronounced hydrophobicity of CD-MON contributed to enhanced selectivity and resolution in separating diverse hydrophobic analytes, such as alkylbenzenes, halogenated benzenes, parabens, and polycyclic aromatic hydrocarbons (PAHs). The maximum column efficiency achieved was 1.5 × 10 5 N/m. Additionally, the CD-MON modified capillary column demonstrated notably high column capacity, with a methylbenzene mass loading capacity of up to 197.9 pmol, surpassing that of previously reported porous-material-based capillaries. Furthermore, this self-constructed column was effectively utilized for PAHs determination in actual environmental water samples, exhibiting spiked recoveries ranging from 93.2 to 107.9% in lake water samples. These findings underscore the potential of CD-MON as an effective stationary phase in separation science. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）