SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Wenjie Liao,Shiqiang Li,Shaoyong Wu,Xiaosong Yang,Bo Liu,Liansheng Ling,Qiuyao Zeng,Ji Zhang
Prostate Cancer Antigen 3, a type of long non-coding RNA, exhibits outstanding specificity as a biomarker for the diagnosis of prostate cancer, offering a highly effective diagnostic indicator, whereas the currently used prostate specific antigen exhibits low specificity, leading to reduced accuracy in prostate cancer diagnosis. Herein, we designed a novel fluorescent sensing platform for targeted detection of PCA3, which integrates the non-specific trans-cleavage activity of the CRISPR/Cas12a with the remarkable fluorescence quenching effect of Gold Nanorods. The Cas12a recognizes and binds to specific sequences of PCA3, thereby activating nonspecific cleavage activity, which cleaves fluorescent reporter probes adsorbed on AuNRs, thus leading to the recovery of fluorescence signals and enabling sensitive detection. The proposed fluorescent sensor exhibits excellent accuracy and convenience for the detection of PCA3 in urine, and a detection limit as low as 1.65 pM was obtained. This sensing system has achieved effective detection of clinical samples of prostate cancer and is expected to provide significant assistance in the screening and therapeutic feedback of prostate cancer in clinical diagnosis.
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Kunqi Liu,Junxia Wang,Jin Wang,Long Kang,Jun Li,Die Liu,Yuan Liu,Qing Tao
To explore the application potential of ceramic waste forms for immobilizing multiple nuclides in high-level waste, a systematic study was conducted on (1- x )Sr 0.5 Zr 2 (PO 4 ) 3 - x Ce 0.5 Sm 0.5 PO 4 ( x = 0, 0.5, and 1) ceramics, focusing on their chemical and irradiation stability. The composite ceramics can simultaneously immobilize multiple nuclides (Sr, Ce, and Sm), while retaining a homogeneous microstructure and high relative density (>97%). Static leaching experiment indicated low normalized leaching rates: LR Sr ∼ 10 -4 -10 -3 g·m -2 ·d -1 , LR Ce ∼ 10 -8 -10 -7 g·m -2 ·d -1 , and LR Sm ∼ 10 -7 -10 -6 g·m -2 ·d -1 . Different leaching behaviors of nuclides (Sr, Ce, Sm) were further identified using the de Groot and van der Sloot model. With 5 MeV Xe 20+ irradiation at a fluence of 2.5×10 15 ions/cm 2 , Sr 0.5 Zr 2 (PO 4 ) 3 phase in the composite ceramics can still maintain part of its crystal structure, while Ce 0.5 Sm 0.5 PO 4 phase tends to amorphize. Interestingly, subsequent TEM observation further vitrificated the Xe 20+ -induced amorphous Ce 0.5 Sm 0.5 PO 4 phase recrystallizes under electron-beam (e-beam) irradiation.
✓ 已验证
DOI
高影响因子
BIOPHYSICAL CHEMISTRY
·
2026
·
IF 2.3
Pingzhang Gao,Yinhua Sui,Xiao-Lan Xie,Rong Tian,Naihao Lu
The health-promoting function of bioactive polyphenols in vitro and in vivo is usually restrained by their low stabilities, which might be counteracted by the use of protein as carriers for these compounds. Demonstration of the binding mechanism of protein-ligand complex is important for the encapsulation and protection of bioactive compounds. In this study, human serum albumin (HSA) was used to investigate its binding characteristic to (−)-epigallocatechin-3-gallate (EGCG, the main component of catechins in green tea), in the presence of free heme (an inducer for cytotoxicity) by multi-spectroscopic analysis and molecular docking. The free radical scavenging capability of bioactive EGCG was retained in the albumin-ligand complex relative to free flavonoid. Albumin-diligand complex (HSA-EGCG-heme) was successfully formed when EGCG and heme was added to protein solution. The toxicity of heme in vascular endothelial cells was declined in the HSA-diligand complex comparison with those of free heme or HSA-heme complex, where the stability of EGCG during storage was improved in the HSA-diligand complex comparison with that in the free EGCG. Our results demonstrate the possibility and advantage of designing albumin protein-based carriers for the protection of active flavonoid and suppression of heme toxicity during their biomedical applications.
✓ 已验证
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2026
·
IF 5.9
Zehui Wei,Shuxian Ge,Yukai He,Lina Mu,Shuangxian Yan,Yu Cheng,Tiantian Gao,Kefan Xie,Miaomiao Zhang,Suhong Wang,Mei Tian
Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early breast cancer diagnosis and therapeutic monitoring. This work presents an ultrasensitive fluorescent probe based on a dual-ligand peptides/UiO-66-NH2@AYG composite for CA15-3 detection. Dual-ligand peptides (GTTFSNYW and MHYLEYPF) target distinct CA15-3 epitopes, enhancing the binding affinity and specificity. The peptides are immobilized on UiO-66-NH2, whose high surface area supports efficient loading of the fluorescent reporter Acridine Yellow G (AYG). This probe demonstrates exceptional performance with a linear detection range of 0.05–1.5 pg/mL (R2 = 0.9981) and an ultralow detection limit of 0.004147 pg/mL. Practical validation in spiked mouse serum shows recovery rates of 91.57–103.7%, while clinical testing with serum samples from 10 cancer patients reveals a strong correlation with ELISA results (r = 0.9944, P < 0.001). This robust platform offers significant potential for cost-effective, early cancer screening in primary healthcare settings.
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2026
·
IF 29.1
Lingjie Liu,Juncai Zhang,Hu Li,Jinrui Cai,Limei Lin,Shuiyuan Chen,Guilin Chen
Severe carrier recombination at the back interface, exacerbated by valence-band misalignment arising from the tunable S/Se composition in Sb 2 (S x ,Se 1−x ) 3 (0 ≤ x < 1) absorbers, remains a critical bottleneck in these solar cells. Here, we address this issue by inserting an Sb 2 S 3 interlayer between the narrow-bandgap Sb 2 (S x ,Se 1−x ) 3 absorber and the PbS hole transport layer (HTL). KPFM and GIWAXS measurements reveal that this Sb 2 S 3 interlayer reduces surface defect density and improves the crystalline quality of the film surface. Moreover, leveraging the complete mutual solubility between Sb 2 S 3 and Sb 2 (S,Se) 3 , TOF-SIMS confirms the formation of a compositionally graded heterojunction from the Se-rich bulk to the S-rich back surface during annealing, creating a continuous energy staircase. Consequently, the hole-extraction barrier is eliminated, the built-in electric field is enhanced, and the depletion region is broadened, accelerating hole collection. This strategy delivers the following performance: for Sb 2 (S,Se) 3 -derived devices, efficiency increases from 6.88% to 9.76%; when extended to the Sb 2 Se 3 -derived case, efficiency rises from 7.77% to 10.03%. These results set new benchmarks for all-inorganic Sb 2 (S x ,Se 1−x ) 3 thin-film solar cells, especially for carbon-electrode devices, and establish a back-interface engineering strategy for Sb 2 (S x ,Se 1−x ) 3 solar cells.
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Jinming Gu,Lang Xie,Dandan Zhou,Yue Yu,Hongliang Huo,Kejian Tian
3-oxo-C12-HSL accumulates markedly under low-temperature stress. • Multiple AHL receptors participate in signal perception and transcriptional control. • Exogenous 3-oxo-C12-HSL enhances key gene expression at 15 and 8°C. • Exogenous 3-oxo-C12-HSL affects energy, antioxidant and fatty acid pathways in cold. Low-temperature stress markedly suppresses the metabolic activity of activated sludge microorganisms, thereby compromising the operational stability of biological wastewater treatment systems. This study investigated associations between 3-oxo-C12-HSL-related quorum-sensing (QS) responses and physiological, transcriptional, and metabolic changes in activated sludge following rapid temperature decline. At 15°C and 8°C, lasI g was upregulated with log 2 FC values of 1.02 and 3.01, respectively, accompanied by 27.79- and 32.87-fold increases in endogenous N-(3-oxo-dodecanoyl)- l -homoserine lactone (3-oxo-C12-HSL) levels, reaching maximum concentrations of 25.05 and 32.90 μg g⁻1 VSS. With 3-oxo-C12-HSL accumulation, QS-related genes, including lasR g , luxR g and sdiA g , were significantly upregulated, with log 2 FC values ranging from 1.13 to 4.84. These changes coincided with the upregulation of genes involved in extracellular polysaccharide biosynthesis, energy metabolism, oxidative phosphorylation, fatty acid metabolism, and antioxidant defense. Meanwhile, differential metabolites associated with the pentose phosphate pathway (PPP), glycolysis (EMP), and the tricarboxylic acid cycle (TCA) increased by 1–104%. These molecular changes were accompanied by pronounced physiological responses, including a 73.37–508.91% increase in extracellular polysaccharides (PS), an increase in ATP from 3278.82 to 7016.70 nmol L⁻ 1 , a 24% decrease in reactive oxygen species (ROS), and a 16% increase in total antioxidant capacity ( T -AOC). Exogenous 3-oxo-C12-HSL supplementation further enhanced these responses, supporting a potential functional role potential functional association in the low-temperature response of activated sludge. Overall, 3-oxo-C12-HSL was associated with coordinated changes in polysaccharide formation, energy metabolism, antioxidant defense, and fatty acid-related responses, suggesting its potential as a candidate QS signal for low-temperature biological wastewater treatment. Download: Download high-res image (286KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2026
·
IF 3.9
Yuantong Yang,Zhao Jiang,Yang Ren,Senqiang Zhu,Rui Liu,Hongjun Zhu
Designed two water-soluble D–π–A fluorescent probes for LFP visualization. • Inert ammonium groups grant high water solubility and LFP enrichment ability. • Probes show weak aqueous emission (TICT) and strong solid-state RIR fluorescence. • Achieved high-contrast imaging of level-3 LFP features on diverse substrates. • Electrostatic adsorption to LFP lipids drives the high-contrast development. High-sensitivity, high-contrast and eco-friendly visualization of latent fingerprints (LFPs) remains a key demand in forensic analysis. Most organic fluorescent developers suffer from poor water solubility and rely on organic solvents, while hydrophilic modifications often lead to fluorescence quenching. Herein, we designed and synthesized two D-π-A fluorescent molecules ( TPA-Q and DMPA-Q ) functionalized with spectroscopically inert alkyl quaternary ammonium groups for LFP development. The alkyl quaternary ammonium groups confer mg/mL-level water solubility and electrostatic enrichment for fingerprint residues without perturbing the intrinsic luminescence. Both compounds exhibit weak fluorescence in aqueous solution due to twisted intramolecular charge transfer (TICT), but intense emission in solid (Φ> 62%) and film states. Using an aqueous atomization method, clear LFPs were successfully developed on various substrates with well-resolved level-3 details (e.g., pores and ridge contours). Notably, TPA-Q excelled on green backgrounds (e.g., Snow beer cans), while DMPA-Q performed better on blue ones (e.g., Pepsi cans, G/G 0 = 3.0), respectively avoiding blue/green substrate interference. These water-soluble fluorescent developers exhibit low background interference, high contrast, and broad applicability when combined with aqueous nebulization and powder-dusting protocols, offering a promising strategy for efficient and nondestructive LFP visualization in practical forensic applications. Download: Download high-res image (298KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Qinghan Chen,Li Wang,Dehui He,Bo Xiao,Xia Ran,Rong Li,Hong Xu,Jian Feng
A novel [email protected] 5 O 7 I/Bi(OH) 3 (AB) heterojunction was prepared and the enhanced photocatalytic antibacterial activity against Escherichia coli ( E. coli ) under visible light excitation was exhibited. 3 %AB (containing 3 wt% of Ag) presented best photocatalytic performance, and all of E. coli cells were inactivated within 5 min under natural solar light irradiation. The photocatalytic antibacterial mechanism of AB heterojunction was corroborated by SEM, live/dead cell staining and SDS-PAGE assay. The destruction of the cell membrane, the leakage of the intracellular protein and the decomposition of the protein were considered as the events occurred in the antibacterial process. The radical capture experiment confirmed that •O 2 – and e – played the dominant role in the destruction of the cell membrane and the decomposition of the protein. The improvement of the photocatalytic activity can be mainly ascribe to the construction of heterojunction, SPR effect resulted from Ag deposition, enhanced visible light absorption and more surface oxygen vacancies. These factors are advantageous to the generation of the photogenerated electrons and holes, and improve the spatial separation efficiency of the photogenerated charge. This study presented a promising [email protected] 5 O 7 I/Bi(OH) 3 heterojunction as the photocatalyst for the potential application in the treatment of the bacterial contamination.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Qing Zhu,Jinxin Wu,Rui Tong,Wenhao Li,Xiuli Hu,Ningchen Tian,Liqing He,Yanwei Li,Zhenyu Wang
Na 4 MnCr(PO 4 ) 3 (NMCP) has been considered as one kind of privileged cathodes with high energy density for sodium ion batteries (SIBs) due to three-electron redox reaction during the insertion/extraction process. Nevertheless, its practical use is restricted by its low intrinsic conductivity and serious Jahn-Teller distortions. Herein, a facile doping strategy was presented to address the above issues, and the effects of different kinds and quantities of transition metal dopants on electrochemical performance of NMCP were systemically studied. The results reveal that Ni50-NMCP/C has the most effective doping composition, showing a discharge capacity of 143.0 mAh g −1 at 50 mA g −1 and long-term cyclic stability with capacity retention of 55.7 % at 1000 mA g −1 over 500 cycles, higher than those of the pristine NMCP (106.6 mAh g −1 at 50 mA g −1 and 38.8 % capacity retention at 1000 mA g −1 ). The substitution of Ni 2+ for Mn 2+ promotes electron conductivity and structural stability, rendering improved electrochemical performance. Moreover, the promising electrochemical performance of Ni50-NMCP/C//HC full cell demonstrates the anode's potential for use. This study indicates a quick way to understand the effects of elemental doping on NMCP and explore novel high energy and high stability cathodes for SIBs.
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Yufei Zhang,Junhua Yun,Hossain M. Zabed,Yuan Dou,Guoyan Zhang,Mei Zhao,Mohammad J. Taherzadeh,Arthur Ragauskas,Xianghui Qi
3-Hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) are value-added chemicals with versatile applications in the chemical, pharmaceutical, and food industries. Nevertheless, sustainable production of 3-HP and 1,3-PDO is often limited by the lack of efficient strains and suitable fermentation configurations. Herein, attempts have been made to improve the co-production of both metabolites through metabolic engineering of Escherichia coli and process optimization. First, the 3-HP and 1,3-PDO co-biosynthetic pathways were recruited and optimized in E. coli , followed by coupling the pathways to the transhydrogenase-mediated cofactor regeneration systems that increased cofactor availability and product synthesis. Next, pathway rebalancing and block of by-product formation significantly improved 3-HP and 1,3-PDO net titer. Subsequently, glycerol flux toward 3-HP and 1,3-PDO synthesis was maximized by removing metabolic repression and fine-tuning the glycerol oxidation pathway. Lastly, the combined fermentation process optimization and two-stage pH-controlled fed-batch fermentation co-produced 140.50 g/L 3-HP and 1,3-PDO, with 0.85 mol/mol net yield.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2023
·
IF 4.3
Chunhui Yi,Fanyong Yan,Xin Wei,Yawei Wu,Xueyu Wang,Jinxia Xu
Based on the fluorescence resonance energy transfer (FRET) principle, a yellow-green light fluorescent probe was designed and synthesized for the detection of neutral red using fluorescein monoaldehyde-3-acetylcoumarin as the fluorophore. The fluorescein moiety in the probe structure is readily interchangeable and heterogeneous in the form of an anion, and the neutral red is a quaternary ammonium salt structure with N. Therefore, the two are bound together in the form of ionic bonds through the mutual attraction of anions and cations. At the same time, FRET occurs, resulting in the absorption of the fluorescence emitted by the probe by the neutral red molecule, leading to a significant quenching of its fluorescence. The probe has a high selectivity for neutral red with a linear range of 0–30 μM and a detection limit of 8. 68 μM. Furthermore, the fluorescent probe has been successfully applied to the detection of neutral red content in real water samples with a recovery of 101 %-115 %. The probe has promising potential in the quantitative detection of neutral red.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2023
·
IF 3.1
Kai Wang,Yunfei Xiao,Changchun Wu,Yimo Feng,Zhipeng Liu,Xi Zhu,Hongjun Zang
3-Acetylamino-5-acetylfuran (3A5AF) is an important nitrogen-containing fine chemical with broad application prospects and high research value. Herein, we report a novel method for the conversion of N-acetyl- d -glucosamine (NAG) to 3A5AF in the choline chloride-based deep eutectic solvents (DESs). The catalytic activities of various DESs have been smoothly screened, and DES 2 (choline chloride/PEG-200/boronic acid = 1/1/0.5) displayed the best catalytic performance. In the absence of any additional solvent, catalyst and additive, product 3A5AF was obtained in 18.3% yield after reacting at 180 °C for 15 min under atmospheric condition. In addition, DES 2 showed a good reusability. The possible reaction pathway was elucidated on the basis of the results of LC-MS and 13 C NMR spectra. This study provided a new perspective for the application of DES in the conversion of chitin biomass.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）