PHYTOMEDICINE
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2026
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IF 11.3
Xu Gao,Xiaofeng Zhu,Yibin Lin,Chengkuan Liu,Qingwen Wang,Xian Lin
Background Developing novel therapeutic targets and innovative drugs for rheumatoid arthritis (RA) remains highly challenging. Despite the involvement of 5-methylcytosine (m5C) RNA methylation in various biological processes, its specific roles in RA and potential applications in drug development remain unclear. Additionally, the role of CX26 in RA progression and its inflammatory effects on fibroblast-like synoviocytes (FLSs) have not been elucidated. Salvianolic acid B (SAB), a bioactive component derived from Salvia miltiorrhiza , possesses anti-inflammatory properties, but its potential in RA treatment has not been completely explored. Purpose This study aimed to clarify the role of m5C-methylated gene CX26 in RA-FLSs and evaluate the therapeutic potential of SAB for RA therapy. Methods Multi-omics analysis was used to determine the contribution of m5C RNA methylation to RA pathogenesis and characterize m5C-methylated genes during RA progression. Methylated RNA immunoprecipitation sequencing and RNA sequencing were employed to analyze the effect of SAB on m5C methylation of CX26 in RA-FLSs. Immunohistochemistry, quantitative real-time PCR, Western blot, Cell Counting Kit-8, 5-Ethynyl-2′-deoxyuridine, and Transwell assays were applied to assess the effects of CX26 and SAB on RA-FLSs. The anti-arthritic activity of SAB was validated in a collagen-induced arthritis (CIA) mouse model. Results This study demonstrated a vital role of m5C RNA methylation in RA progression. CX26 was identified as an m5C-methylated gene that was selectively overexpressed in RA-FLSs. The CX26 levels in the synovium of patients with RA demonstrated significant associations with an activated immuno-inflammatory phenotype, clinical features, and treatment outcomes. Moreover, the CX26 mRNA m5C methylation, stability and expression were elevated by TNF. CX26 augmented inflammation-induced proliferation, migration, and invasion of RA-FLSs via activating the IκBα/NF-κB signaling. SAB reduced m5C methylation of CX26 mRNA and impair CX26 expression, thus abrogating CX26/IκBα/NF-κB signaling–induced pathological phenotypes of RA-FLSs. Furthermore, computational modeling and the CIA model confirmed that SAB alleviated RA progression. Conclusions This cross-validated study, based on multi-omics analysis, outlines the potential of CX26 as a highly selective target and SAB as a novel therapeutic candidate for RA treatment.
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Biomed Research International
·
2026
·Vol. 2026
·期 1
·1031453
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IF 2.9
Junling Wang,Xianghong Kong,Huiping Xu,Wei Zhang,Peng Peng
Background Entecavir (ETV) combined with pegylated interferon alpha (PEG-IFN α ) improves chronic hepatitis B (CHB) functional cure rates, but therapeutic heterogeneity and underlying metabolic mechanisms remain unclear. This study used untargeted metabolomics to identify metabolic signatures, mechanisms, and predictive biomarkers of functional cure with ETV-PEG-IFN α . Methods Thirty-eight CHB patients were grouped into ETV monotherapy (Group E, n = 12) and ETV-PEG-IFN α combination therapy (Group Z, n = 26); Group Z was subdivided into cured (Group A, n = 13) and noncured (Group B, n = 13). Serum metabolomic profiling, multivariate statistics, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified differential metabolites. A random forest model was built using key metabolites. Results Three hundred eighty-eight metabolites were identified. Four differential metabolites distinguished Group A and B (upregulated guanidinoacetic acid, uracil 5-carboxylate; downregulated L-methionine S-oxide, oleamide), enriching amino acid metabolism pathways. Nine differential metabolites between Group E and Z implicated amino acid, immune, and fatty acid pathways. The random forest model based on the four Group A/B metabolites showed 88.5% cross-validation accuracy (AUC = 0.920), with L-methionine S-oxide and oleamide as key predictors. Conclusions This study reveals metabolic rewiring in CHB functional cure via ETV-PEG-IFN α therapy, involving energy metabolism, oxidative stress, and immunomodulation, based on which we propose a tentative metabolism-immunity synergy model to guide future research. Key metabolites, especially L-methionine S-oxide and oleamide, show exploratory predictive potential for functional cure that warrants further validation in independent cohorts.
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RUSSIAN JOURNAL OF GENERAL CHEMISTRY
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2025
·Vol. 95
·期 9
·2457-2467
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IF 0.8
Sun Yanhua,Kong Xiangyang,Xi Shuoyan,Xu Zhuoyue,Zhang Yuhan
Salvianolic acid (SAB) is a natural flavonoid compound possessing significant pharmaceutical value. However, the extraction and purification of SAB from natural products are hindered by the complexity and low concentration of matrix components. In this research, a new surface imprinted polymer with core-shell structure, denoted as ZIF-8@MIPs, was successfully developed using porous metal-organic frameworks (MOF) ZIF-8 as the scaffold. ZIF-8@MIPs exhibited remarkable selective extraction and enrichment of SAB from Salviorrhiza . Series of characterizations and adsorption experiments demonstrated that ZIF-8@MIPs possessed a typical core-shell octahedral structure with a high specific surface area. The resultant high-density imprinted sites allowed the ZIF-8@MIPs to exhibit a high adsorption capacity (46.7 mg/g) and exceptional selectivity (IF = 3.11) for SAB, while achieving adsorption equilibrium within 90 min. Notably, the ZIF-8@MIPs maintained an adsorption capacity of 89.2% for SAB after 7 cycles of adsorption-desorption experiments, indicating good reusability. Furthermore, a solid-phase extraction column was established utilizing the ZIF-8@MIPs as the adsorbent, and the content of SAB in the Salviorrhiza extract was determined by combining this with HPLC. The spiked recovery rate ranged from 87.70 to 92.90%, with RSD of 2.87–3.43%, demonstrating the reliability and reproducibility of this method. In conclusion, the ZIF-8@MIPs developed in this study offer a promising approach for the selective extraction of active flavonoids from natural matrices, with potential application in application prospects.
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Materials
·
2023
·Vol. 16
·期 7
·2606
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IF 3.4
Wei Cui,Jiahui Fang,Yuanyuan Wan,Xueyu Tao,Litong Guo,Qiyan Feng
In this study, an E-fenton oxidation system based on Co-N co-doped carbon nanotubes (Co-N-CNTs) was designed. The Co-N-CNTs system showed fast degradation efficiency and reusability for the degradation of rhodamine B (RhB). The XRD and SEM results showed that the Co-N co-doped carbon nanotubes with diameters ranging from 40 to 400 nm were successfully prepared. The E-Fenton degradation performance of Co-N-CNTs was investigated via CV, LSV and AC impedance spectroscopy. The yield of H2O2could reach 80 mg/L/h within 60 min, and the optimal voltage and preparation temperature for H2O2yield in this system was −0.7 V (vs. SCE) and 800 °C. For the target pollutant of RhB, the fast removal of RhB was obtained via the Co-N-CNTS/E-Fenton system (about 91% RhB degradation occurred during 60 min), and the •OH played a major role in the RhB degradation. When the Fe2+concentrations increased from 0.3 to 0.4 mM, the RhB degradation efficiency decreased from 91% to about 87%. The valence state of Co in the Co-N-C catalyst drove a Co2+/Co3+cycle, which ensured the catalyst had good E-Fenton degradation efficiency. This work provides new insight into the mechanism of an E-Fenton system with carbon-based catalysts for the efficient degradation of RhB.Keywords:carbon nanotubes;E-Fenton;rhodamine B;degradation;mechanism
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FISH & SHELLFISH IMMUNOLOGY
·
2023
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IF 4.7
Weijie Chen,Jing Hu,Jianchang Huang,Qin Liu,Qiyao Wang,Yuanxing Zhang,Dahai Yang
Bacterial disease is one of the important factors leading to economic losses in the turbot ( Scophthalmus maximus ) cultivation industry. T lymphocytes are major components of cellular immunity , whereas B lymphocytes produce immunoglobulins (Ig) that are key elements of humoral immune responses against infection. However, the genomic organization of genes encoding T-cell receptors (TCR) and immunoglobulin heavy chains (IgHs) in turbot remains largely unknown. In this study, abundant full-length transcripts of TCRs and IgHs were sequenced by Isoform-sequencing (Iso-seq), and we investigated and annotated the V, D, J and C gene loci of TCRα, TCRβ, IgT, IgM and IgD in turbot. Furthermore, through single-cell RNA sequencing (scRNA-seq) of blood leukocytes, we confirmed that these identified TCRs and IgHs were highly expressed in T/B cell clusters, respectively. Meanwhile, we also identified the IgM + IgD + B and IgT + B cells with differential gene expression profiles and potential functions. Taken together, our results provide a comprehensive understanding of TCRs and IgHs loci in turbot, which will contribute to evolutionary and functional characterization of T and B lymphocytes in teleost.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2023
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IF 4.3
Minzhang Li,Wei Wang,Rajendran Ramachandran,Fuming Chen,Zong-Xiang Xu
Exploring highly efficient photocatalysts is critical for integration of photocatalysis and peroxymonosulfate (PMS) activation. Herein, N-CuMe 2 Pc/Siloxene nanocomposites were fabricated by immobilizing the N-CuMe 2 Pc nanorods onto the surface of ultrathin Siloxene nanosheets to activate PMS for efficient photodegradation of (Rhodamine B) RhB. The prepared nanocomposites possess excellent photoabsorption capability and separation efficiency of photogenerated carriers. As expected, the nanocomposites exhibit superior catalytic activity and stability in PMS activation toward RhB photodegradation with highest removal efficiency of 95.3%, which is higher than that of Siloxene and N-CuMe 2 Pc. The active species in photocatalytic process and degradation pathways were determined through radical quenching studies. The reaction mechanism was systematically illustrated based on the intermediates detection and band structures. This study provides an experimental basis for further understanding of the photocatalytic oxidation behavior of metal phthalocyanine modified two-dimensional semiconductor materials, and is helpful to promote the research and application of photocatalytic degradation of refractory organic pollutants.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 7
·6844
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IF 5.6
Guoying Zhou,Jiayan Zhu,Liang Jin,Jing Chen,Ruojiao Xu,Yali Zhao,Tingzi Yan,Haitong Wan
Inflammatory dysfunction and angiogenesis inhibition are two main factors leading to the delayed healing of diabetic wounds. Hydrogels with anti-inflammatory and angiogenesis-promoting effects have been considered as promising wound care materials. Herein, a salvianolic acid B (SAB)-loaded hyaluronic acid (HA) self-healing hydrogel (HA/SAB) with anti-inflammatory and pro-angiogenesis capacities for diabetic wound healing is reported. The HA hydrogel was prepared via the covalent cross-linking of aldehyde groups in oxidized HA (OHA) and hydrazide groups in adipic dihydrazide (ADH)-modified HA (HA-ADH) with the formation of reversible acylhydrazone bonds. The obtained HA hydrogel exhibited multiple favorable properties such as porous structures, excellent self-healing properties, a sustainable release capacity of SAB, as well as excellent cytocompatibility. In addition, the effects of the SAB-loaded HA self-healing hydrogel were investigated via a full-thickness skin defect model using diabetic rats. The HA/SAB hydrogel showed enhanced skin regeneration effects with accelerated wound closure, shorter remaining dermal space length, thicker granulation tissue formation, and more collagen deposition. Furthermore, reduced inflammatory response and enhanced vascularization were found with HA/SAB2.5 hydrogel-treated wounds, indicating that the hydrogel promotes diabetic wound healing through the promotion of anti-inflammation and angiogenesis. Our results suggest that the fabricated SAB-loaded HA self-healing hydrogel is promising as a wound dressing for the treatment of diabetic wounds.Keywords:diabetic wound healing;self-healing hydrogel;salvianolic acid B;anti-inflammation;angiogenesis
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Shengyu Zhu,Ruchika Hansanie Ukwatta,Xingru Cai,Yalu Zheng,Feng Xue,Chen Li,Luxin Wang
This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus , Escherichia coli , and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm). The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB. In addition, the elasticity and the thermal stability of the film were enhanced. The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation. The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition. Moreover, the antibacterial ability was enhanced with the increase in EB concentration. Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.
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ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 7
·2210860
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IF 19
Chenya Zhuo,Huiming Kong,Ke Yi,Chun-Wei Chi,Jiabing Zhang,Ran Chen,Haixia Wang,Caixia Wu,Yeh-Hsing Lao,Yu Tao,Mingqiang Li
Chronic hepatitis B infection remains incurable due to the stable presence of various forms of hepatitis B virus (HBV) genome, especially the HBV covalently closed circular DNA (cccDNA). The emergence of clustered regularly interspaced short palindromic repeat (CRISPR) technology provides a new opportunity to potentially cure the HBV infection. However, the efficiency and specificity remain unsatisfactory, especially for nonviral CRISPR/Cas9 delivery. To tackle these, a liver-specific CRISPR/Cas9 magnetic nanosystem FMNP pAG333/sgXPP is constructed based on fluorinated polyethylenimine-coated magnetic nanoparticles and liver-specific ApoE.HCR.hAAT promoter-driven Cas9-T2A-EGFP plasmid with dual sgRNAs. The elaborate system enables magnetic field-induced spatially specific distribution and hepatocyte-specific promoter-driven liver-specific gene editing. Moreover, this CRISPR/Cas9 magnetic nanosystem is designed to disrupt the two conserved sites in X opening reading frame and Pol opening reading frame of the HBV genome, thereby significantly inactivating the HBV genome without showing off-target effects. Treatment with FMNP pAG333/sgXPP for 7 days reduces serum HBsAg levels by 76% with a total editing efficiency of ≈20% in the two conserved sites. Collectively, this study demonstrates spatiotemporal liver genome editing as well as the feasibility of applying a nonviral CRISPR/Cas9 vector for HBV treatment, which may open up a new application for CRISPR therapeutics.
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International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 23
·15551
·
IF 4.614
Renguo Liu,Xue Li,Jinhui Huang,Haoliang Pang,Qiongfang Wan,Kun Luo,Ya Pang,Lingyu Wang
g-C3N4/Ag3PO4/TiO2nanocomposite materials were loaded onto a polyvinylidene fluoride (PVDF) membrane using a phase inversion method to obtain a photocatalytic flat membrane for dye removal. The morphology, structure, and photocatalytic activity of the g-C3N4/Ag3PO4/TiO2nanoparticles and composite membrane were evaluated. The g-C3N4/Ag3PO4/TiO2/PVDF membrane exhibited superior morphology, hydrophilic properties, and antifouling performance compared with the raw PVDF membrane. Four-stage filtration was performed to evaluate the self-cleaning and antifouling capacity of the g-C3N4/Ag3PO4/TiO2/PVDF membrane. Upon irradiating the composite membrane with visible light for 30 min, its irreversible fouling resistance (Rir) was low (9%), and its flux recovery rate (FRR) was high (71.0%) after five filtration cycles. The removal rate of rhodamine B (RhB) from the composite membrane under visible light irradiation reached 98.1% owing to the high photocatalytic activity of the membrane, which was superior to that of raw PVDF membrane (42.5%). A mechanism of photocatalytic composite membranes for RhB degradation was proposed. Therefore, this study is expected to broaden prospects in the field of membrane filtration technology.Keywords:g-C3N4/Ag3PO4/TiO2nanocomposite;photocatalytic membrane;self-cleaning;membrane fouling;dye
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Ziqi Ren,Lu Fang,Miao Cui,Tianming Lv,Huifang Wu,Ziyi Feng,Zhaochi Feng,Changgong Meng,Limin Ren
Catalyst engineering plays a key role in bridging gaps between the structure of the Lewis acid center and its corresponding catalytic performance. In this regard, work that has been done in tuning active sites of Hf-containing zeolites, which hold promising catalytic activity for a variety of hydrogen-shift reactions, is far from complete. Herein, a Hf-B-BEA catalyst was rapidly prepared (within hours) by a hydrothermal semi-reconstruction strategy, involving partial framework etching of parent B-BEA and Hf grafting during the reassembling process. Then, an acid treatment was performed for expelling the boron atoms from the framework; more importantly, Hf migration into the silanol nests was triggered. The acid treatment worked powerfully as the driving force for transforming the semi-framework Hf into well-defined tetrahedrally coordinated framework Hf, thus endowing Lewis acidity of the obtained Hf-BEA. Consequently, this operation greatly boosted the Meerwein–Ponndorf–Verley reduction performance of Hf-BEA, which was almost inactive over the pristine Hf-B-BEA. The rational regulation of Hf sites provides new concepts in designing Hf-containing zeolites and understanding the structural–activity relationship.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2023
·
IF 5
Ling Yang,Linzi Li,Qian Lu,Lingfeng Li,Chun Xie,Fakun Jiang,Hongbing Li,Ai Zhao,Qian Wang,Wenyong Xiong
As a global epidemic disease, obesity causes dysfunction of glucose and lipid metabolism leading to persistently high morbidity and mortality. Given the difficulty to achieve and maintain weight loss through controlling diet and physical exercise, pharmacotherapy is considered an effective treatment for obesity. This investigation revealed that alisol B, a triterpene monomer isolated from the classical Chinese medicine Alisma orientale (Sam.) Juzep, functioned in suppressing adipogenesis and reducing the mass of subcutaneous adipose tissue, resulting in the reduction of weight gain, and improvements of hyperglycemia, hyperlipidemia, and insulin resistance in HFD-induced obese mice. In consistent to the results, alisol B also significantly inhibited adipocyte differentiation and maturation in vitro . Furthermore, our data revealed that the effects of alisol B on adipogenesis were mediated by LKB1-AMPK signaling pathway. In total, alisol B could be a potential lead compound which contributes to the improvement of obesity-related metabolic disorders.
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