Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Sun Xiaolong,Hu Jiahuan,Wang Yifeng,Luo Xi,Huang He,Fu Yongqian
The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N2 sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid and alkali, temperature and organic solvents. Furthermore, the bio-catalyst could be separated easily using a magnet, and the reusability was once considerably expanded with 80% of enzyme activity last after eight rounds of recycling. Therefore, LDH/MNPs@MAF-7 could be used as a potential biocatalyst for the biosynthesis of D-PLA due to its good stability and recovery properties.
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高影响因子
HIGH PERFORMANCE POLYMERS
·
2019
·
IF 1.584
Yinhong Liu,Yuhan Wei,Yongshuang Bian,Shengjiao He,Xin Wang,Xin Liu,Chenglei Li,Qi Wu,Xianyin Zeng,Hanguang Wang,Kuan Liu
Design and synthesis of highly selective fluorine ion probes become particularly important owing to the specific role of fluorine ion in chemical and biomedical progresses. As a new-type of fluorescent material, conjugated polymers with unique photometric properties have been widely researched by scientists in the field of the fluorescence sensors. In this study, the polymer PBTPV-OSi containing benzothiazole moiety is synthesized via palladium-catalyzed Heck coupling reaction. This polymer not only exhibits good solubility in organic solvents but also shows high selectivity for fluorine ion detection in comparison to other anions. Upon addition of F− to PBTPV-OSi solution, Si–O cleavage of PBTPV-OSi leads to the fluorescence quenching of the polymer in tetrahydrofuran dramatically, and the detection limit is 8 × 10−6 mol/L. Moreover, besides detecting fluorine ion from organic phase, the probe can also effectively detect potassium fluoride from inorganic phase. More importantly, a naked-eye detectable chromogenic and fluorogenic dual response to fluorine ion (F−) can be visibly noted and the detection process of fluorine ion is relatively fast.
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JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2025
·
IF 4.9
Heng Zhou,Yueming Long,Fangmei Yu,Chenhui Ji,Li Gui,Yunxia Lu
Resveratrol (RSV) ameliorates endothelial dysfunction (ED) primarily through sirtuin 1 (SIRT1). Increasing evidence shows pyroptosis as a novel mechanism in palmitic acid (PA)-induced ED. p66Shc is an adaptor protein involved in oxidative stress. However, whether RSV attenuates the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome via p66Shc remains elusive. This study aims to evaluate whether the anti-pyroptotic effect of RSV and the SIRT1 inhibitor EX527 are related to p66Shc. High-fat diet (HFD) induced obesity in mice, and RSV was administered intragastrically with 400mg/kg/d for 22 successive weeks. The serum levels of interleukin-1β (IL-1β) and IL-18 were analyzed, and the expression of related proteins were assayed with immunohistochemistry in the thoracic aorta. human umbilical vein endothelial cells (HUVECs) were induced by PA, then treated with RSV and EX527 respectively, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP) and expression of p66Shc, NLRP3, GSDMD and pyroptosis-related genes were assayed. RSV administration ameliorated endothelial cell pyroptosis by decreasing serum IL-1β and IL-18, the expression of NLRP3, p66Shc, and gasdermin D (GSDMD), and increasing the expression of SIRT1 in the HFD-treated thoracic aorta. PA promoted GSDMD-mediated endothelial cell pyroptosis by ROS production, LDH release, decreased MMP and SIRT1 expression, increased expression of p66Shc and activation of the NLRP3 inflammasome in a dose-dependent manner. RSV attenuated PA-induced pyroptosis, whereas EX527 reversed the antipyroptotic effect of RSV in PA-treated HUVECs. Our results suggested a new mechanism that RSV improves PA-induced pyroptosis in endothelial cells via the SIRT1-p66Shc-NLRP3 pathway.
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TOXICOLOGY AND INDUSTRIAL HEALTH
·
2021
·
IF 2.273
Chong Wang,Yao Shu,Li Xu,Qiling Liu,Bei Zhang,Hong Zhang
Bisphenol A (BPA), a component of polycarbonate and epoxy resins, has been reported to induce learning and memory deficits. However, the mechanisms have not been fully elucidated. Growing evidence has suggested that N-methyl-d-aspartate receptors (NMDARs) are involved in cognitive impairments. In this study, BPA was administered to female Sprague–Dawley rats (six per dose group) at concentrations of 0 (control), 4, 40, and 400 μg/kg·body weight/day from gestation day 1 through lactation day 21. Spatial learning was evaluated using the Morris water maze on postnatal day 22. Expression levels of NMDARs were determined using real-time polymerase chain reaction and Western blot. The results showed that male offspring exposed to BPA exhibited increased latency in reaching the platform and reduced time in the target quadrant, and the number of crossing the platform was less, as compared with the control group. The mRNA and protein expression levels of NMDARs in the hippocampus were significantly downregulated when compared with the control group of male offspring. The data showed that maternal exposure to BPA at low dosage can cause cognitive deficits in male rat offspring, probably due to a decrease in NMDARs in the hippocampus.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Chunli Fan,Xinshuo Wang,Min Zhang,Yaqi Hou,Shuqin Xia,Zhongxiang Fang
In this study, octenyl succinic acid sodium starch (OSAS) decorated with chitosan (CS) of different molecular weights (50–150 kDa) and concentrations (10–30 mg/mL) was used to stabilize an emulsion coencapsulating with vitamin A (V A ) and vitamin D (V D ). The effect of CS decoration on the thermal and UV stability of the emulsion, as well as the underlying mechanism, was elucidated. The incorporation of CS increased the retention rates of V A and V D by 11.36–25.52 % and 3.65–20.54 %, respectively, when exposed to 100 °C for 30 min, and by 13.35–33.05 % and 15.97–37.49 %, respectively, under ultraviolet (UV) exposure for 12 h, respectively. The OSAS/CS complexes were absorbed at the oil–water interface through electrostatic interactions and hydrogen bonding, forming thick interfacial film barriers, and regulating emulsion viscoelasticity to achieve protection against thermal and UV damage. CS decoration increased the thickness, relative crystallinity, and thermal degradation resistance of the interfacial films to mitigate thermal interference with the emulsion. The OSAS/CS complex barriers shielded UV by forming longer molecular chains or ring structures at the interface, enhancing amide functionality, and promoting intermolecular hydrogen bonding. This research could provide a reference for designing practical delivery systems for heat-sensitive and UV-sensitive nutrients like V A and V D .
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2023
·Vol. 32
·期 2
·536-547
·
IF 5.3
Chen Weishuo,Zhong Channa,Li Shuangcheng,Wen Die,Zhou Dongdong,Shao Jun,Chen Shuiliang,Hou Haoqing,Xiang Sheng
The blends of poly(D-lactic acid) and poly(L-lactic acid) (PDLA/PLLA) produce the stereocomplex crystals (SC) with a melting temperature of ~ 250 °C, which exhibits higher crystallization rate and mechanical strength than PDLA or PLLA. The SC was also reported as an effective nucleator for PLA homochiral crystals (HC). In the PDLA/PLLA blends, the SC would develop at first in most of cases. However, the morphology of SC in the asymmetric blends were rarely reported, and the effect of the SC morphology on the subsequently crystallization of HC was not clear yet. In this study, the PDLA/PLLA blends with dissimilar weight ratios were prepared by three methods, i.e. , electrostatic spinning, solution casting, and melted-cooling, and the SC with different morphologies were achieved. Then, the crystallization behavior of HC under the existence of SC was studied. Results indicated that the SC morphology exerted diverse effects on the formation of HC. The SC with fibrous and banded morphology produced by electrostatic spinning presented great limitation for the formation of HC. The SC with spherical morphology developed in the solution casting films enhanced the formation of HC. The SC with network structure produced in the melted-cooling specimens accelerated the crystallization rate of HC significantly. This investigation indicated that not only the content of SC, but also the morphology of SC would influence the crystallization behavior of HC, which would make a deep understanding for the crystallization behavior of PDLA/PLLA specimens.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Rajapriya Andavar,Mameng Yang,Cunpeng Duan,Anuj Kumar,Yanzhi Sun,Junqing Pan
Industrial-level alkaline water electrolysis requires high-efficiency catalysts to achieve rapid water dissociation and hydrogen intermediate migration at an ampere-level current density. However, conventional alloy catalysts lack spatially organized, abundant site functions, resulting in poor coupling between alkaline electrolysis steps. Here, we report a new NiCoFeCuMoW high-entropy alloy (HEA) catalyst prepared by pulse electrodeposition, featuring efficient mass and charge transport. The catalyst features a three-dimensional hierarchically porous framework with tailored surface atomic configurations and chemically differentiated multimetallic motifs, enabling abundant accessible active sites, accelerated transport, and cooperative regulation of reaction intermediates. Density functional theory (DFT) calculations show that Mo/W-richdomains preferentially facilitate H 2 O dissociation, whereas adjacent NiCoFeCuMoW ensembles optimize hydrogen diffusion and intermediate stabilization via regulated d-band states, thereby establishing an efficient dissociation-diffusion cascade. The NiCoFeCuMoW HEA delivers overpotentials of only 36 mV within the hydrogen evolution reaction (HER) while exhibiting 226 mV in the oxygen evolution reaction (OER) operating in alkaline electrolytes at 10 mA cm −2 . Amazingly, the cell delivers an ultra-low electrolyte potential of 1.57 V at 120 °C and 1 A cm −2 , owing to greatly enhanced charge-mass transport and perturbed hydrogen-bond networks in the over-boiling environment, providing a scalable HEA design strategy for ampere-level industrial water splitting. The boosted electrocatalytic acitivity of new HEAs alloy catalyst under over-boiling environment. Download: Download high-res image (204KB) Download: Download full-size image
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-8
·
IF 5.7
Yu Yao,Li Yansong,Xu Ning,Zha Yonghong,Bai Xue,Liu Mingyuan,Zhou Yu,Liu Xiaolei
Fumonisin B1 (FB1) is one of the important mycotoxins posing health risks in the area of food safety. A sensitive fluorescence ratio immunoassay has been established for FB1 based on the growth of monodispersed 2-D MnO 2 nanosheet on an individual gold nanoparticle (AuNP@MnO 2 ). FB1 competed with the coated FB1-BSA to bind the FB1 monoclonal antibody. After a washing step, alkaline phosphatase-labeled goat anti-mouse IgG (ALP-IgG) with high catalytic activity was combined with FB1 monoclonal antibody. ALP reacts with ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA), which decomposes AuNP@MnO 2 to dehydroascorbic acid (DHAA). O-Phenylenediamine dihydrochloride (OPD) is oxidized to yellow-fluorescent substrate of 2,3-diaminophenazine (DAP) (excitation, 423 nm; emission, 570 nm) by AuNP@MnO 2 . Meanwhile, OPD can also be reduced to blue fluorescent substrate of OPDred (excitation, 350 nm; emission, 430 nm) by DHAA. The content of FB1 can be determined by fluorescence ratio of blue/yellow. The limit of detection (LOD) of the fluorescence ratio immunoassay for FB1 was 0.06 ng mL −1 , and the linear range was from 0.25 to 60.00 ng mL −1 . The effectiveness of the assay was verified in real maize samples, and satisfactory recoveries were attained. The correlation coefficient of these results between the fluorescence ratio immunoassay and commercial ELISA kit was 0.9999. This method provides a sensitive and selective tool for the detection of FB1 in maize samples. Graphical Abstract
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Han Zhang,Heping Cui,Xue Xia,Shahzad Hussain,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
Lysine (Lys) glycated by xylose (Xyl) at α-NH2 [Nα(1-deoxy-D-xylulos-1-yl)lysine (Nα-Xyl-Lys ARP)] or ε-NH2 [Nε-(1-deoxy-D-xylulos-1-yl)lysine (Nε-Xyl-Lys ARP)] significantly impacted the thermal degradation pathways of Amadori rearrangement products (ARPs). Nα-Xyl-Lys ARP was found to undergo retro-aldolization on the sugar fragment more readily to form glyoxal/methylglyoxal than Nε-Xyl-Lys ARP. Furans and pyrazines formation during the degradation of the diglycated lysine [Nα,Nε-di(1-deoxy-d-xylulos-1-yl)lysine (Nα,Nε-di-Xyl-Lys ARP)] was delayed at 120 °C relative to Nε-Xyl-Lys ARP. This was attributed to the complex degradation of Nα,Nε-di-Xyl-Lys ARP, which slowed the substantial formation of deoxypentosones and the effective release of Lys. At 140 °C, the dual glycated Nα,Nε-di-Xyl-Lys ARP was more conducive to promoting the redistribution of electrons and facilitating molecular rearrangement. This accelerated the efficient decomposition of dual glycated groups in Nα,Nε-di-Xyl-Lys ARP and enabled glyoxal to actively participate in Strecker degradation. Thus, the production of furans and pyrazines was substantially increased, and the variety of pyrazines was expanded from three types to eight types. An appropriate increase to pH 7.5 effectively avoided the overprotonation of hydroxyl and amino groups (pH 5.5), simultaneously enhancing furans and pyrazines yield while minimizing the formation of pyridines under alkaline conditions.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Zeng Yu,Jianbota Hanahati,Li Xuepeng,Zhang Hong,Lv Xuepeng
Alkali induction promotes the formation of fibrous gel network structure. • Increasing pH weakens the hydrogen bond and hydrophobic interactions of the gel. • To reveal the link between gel structure formed by different pH and VD 3 encapsulation stability. • Composite gel structural compactness inhibits VD 3 release during digestion. This study explored the effects of ultrasound-assisted alkaline treatment between the structure and vitamin D₃ (VD₃) encapsulation efficiency, together with in vitro release behavior of heat-induced egg yolk granule/whey protein composite gels. Results showed that the alkaline environment remarkably modulated the intermolecular conformation and aggregation behavior of composite proteins. With increasing pH, protein unfolding extent augmented, while surface hydrophobicity first decreased and then increased, peaking at pH 11. Enhanced electrostatic repulsion improved system dispersibility, accompanied by reduced particle size and elevated absolute zeta potential. Gel microstructure evolved from a spongy homogeneous porous matrix at pH 7 to a fibrous network at pH 11. Moreover, higher pH resulted in finer fibers, increased pore density, and enlarged average pore size. These changes were accompanied by the strengthening of disulfide bonds and hydrophobic interactions, which contributed to the formation of a robust elastic-dominated gel network. The highest VD 3 encapsulation efficiency (95.74%) was achieved at pH 9, where gels also exhibited the optimal VD₃ retention rate (26.18%) after 28 days of storage at 4 °C. In vitro digestion experiments indicated that pH 11 gels displayed the fastest release kinetics during gastric and intestinal phases, with a final cumulative release rate of 89.82%. In contrast, pH 7 and 9 gels showed sustained-release features, and their release behaviors were well fitted to kinetic models, respectively. In conclusion, ultrasound-assisted alkaline treatment optimizes composite gel network structure and functional attributes via precise pH modulation, providing a theoretical foundation for stable storage and controlled release of VD₃ in food matrixes.
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INORGANIC CHEMISTRY
·
2026
·
IF 4.6
Lin Wei,Yang Zhibin,Tao Jin-Gang-Lu,Zhao Qianqian,Liu Fuyu,Li Wanshan,Feng Renfei,Zhang Yu,Fu Xian-Zhu,Luo Jing-Li,Zhao Bin
Developing efficient and durable electrocatalysts for
alkaline
seawater hydrogen evolution remains challenging because of sluggish
water dissociation kinetics. Herein, we report a defect-rich d-p orbital-hybridized
Os/o-CNTs electrocatalyst ( dph -Os def /o-CNTs)
prepared by rapid room-temperature NaBH 4 reduction. The
partially oxidized CNT support anchors defective Os nanoclusters and
induces C–Os/C–O–Os electronic coupling, generating
electron-deficient Os sites with modulated 5d electronic structures.
In alkaline simulated seawater (1 M NaOH + 3.5 wt % NaCl), dph -Os def /o-CNTs requires only 91 mV to reach
100 mA cm –2 and exhibits a Tafel slope of 19 mV
dec –1 , with stable operation over 350 h and 10,000
CV cycles. In situ Raman spectroscopy, AIMD simulations, and DFT calculations
reveal that Os vacancies reorganize interfacial water into an ordered
H-bond network, promote O-down water adsorption, and reduce the water
dissociation barrier from 1.12 to 0.56 eV. Moreover, an Al | OH – | H 2 primary cell using dph -Os def /o-CNTs as the cathode delivers 1.41 V and 10.92
mW cm –2 , enabling simultaneous hydrogen generation
and electricity output.
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Reaction Chemistry & Engineering
·
2020
·Vol. 5
·期 3
·531-538
·
IF 3.441
Xiaolin Pei,Jiapao Wang,Haoteng Zheng,Pengfei Cheng,Yifeng Wu,Anming Wang,Weike Su
D-(−)-Pantolactone (D-PL) is the key chiral intermediate used to synthesize calcium D-pantothenate. In a previous study, a conjugated polyketone reductase (CduCPR) was discovered to efficiently catalyse ketopantolactone (KPL) to D-PL with a high stereoselectivity. Nevertheless, this method was limited due to the expensive coenzyme, nicotinamide adenine dinucleotide phosphate hydrate (NADPH), and the poor stability of substrate KPL in pure aqueous buffer. Here, to overcome these problems, we developed a whole-cell biotransformation process to produce D-PL in a biphasic reaction system. Recombinant CduCPR and glucose dehydrogenase (BsuGDH) were co-expressed in Escherichia coli to simultaneously achieve the synthesis of D-PL and the regeneration of NADPH. The biphasic reaction system contained 15% dichloromethane (v/v) which significantly inhibited the undesirable hydrolysis of KPL. Approximately, 2.8% of 100 mM KPL was spontaneously hydrolysed to ketopantoic acid in the biphasic system, which was lower than 54% of pure aqueous buffer. The biphasic reaction system was optimized and scaled up to prepare D-PL. In a fed-batch biphasic reaction, the substrate KPL was continuously fed into the reactor after a batch reaction. The D-PL concentration reached 0.77 mol L−1 in the reaction mixture at 7 h, and its enantiomeric excess was 99%. This study presented an alternative method to asymmetrically synthesize enantiopure D-PL for further industrial application.
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