TALANTA
·
2026
·
IF 6.7
Lu Shi,Jiayi Jin,Hanyao Liu,Na Wu,Chuyun Xu,Jianghao Bu,Xiaoting Zhao,Shuai Li,Yanxia Qi,Shanshan Li,Hua Wang
A label-free intelligent fluorometric array based on DNA conformational diversity is developed. • Thioflavin T intercalation smartly transduces metal-induced DNA conformational changes into fluorescence signals. • The cost-effective platform successfully detects multiple metal ions in lake and tap water. Multiplex detection of metal ions is often challenged by their similar charge states and ionic radii, which interfere with for simultaneous analysis and cause the low selectivity of conventional lock-and-key recognition elements. Herein, a label-free and intelligent fluorometric array has been developed for simultaneously probing multiplexed metal ions by utilizing the conformational diversity of DNA structures, including G-triplex, G-quadruplex, and dimeric G-quadruplex DNA. The sensing mechanism relies on thioflavin T intercalation, which converts metal-binding-induced DNA conformational changes into quantifiable fluorescence signals. The resulting fluorescence fingerprints were first visualized using principal component analysis and subsequently classified using supervised machine-learning algorithms. A comparative evaluation of multiple models reveals that the support vector machine achieves the highest classification performance for selective identification of multiple metal ions. The developed fluorometric array can accurately distinguish the samples containing various metal species (Fe 3+ , Co 2+ , Hg 2+ , Ni 2+ , Zr 4+ , Ag + , and Zn 2+ ) over a wide range of concentrations (10-200 μM), with a 100% accuracy in blind validation tests. Subsequently, the feasibility of the array for detecting multiple metal ions in mixtures, as well as in lake water and tap water, is also demonstrated. As compared with most current analysis technologies, this method offers the desirable advantage of simultaneous discrimination of multiple metal ions including the heavy and non-heavy ones. Therefore, this study establishes a powerful, efficient, and cost-effective platform for the simultaneous and intelligent detection of multiple metal ions, showing strong potential as a rapid on-site screening tool for preliminary risk assessment of metal contamination. A label-free fluorometric array based on diverse DNA structures enables multiplexed metal ion discrimination. Download: Download high-res image (296KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2026
·
IF 3.2
Ye Yuan,Chen Chen,Shengjie Zeng,Lianju Shen,Qing Jiang,Junke Wang
DBP exposure impaired BTB integrity in prepubertal male mice. • MBP induced G1-phase arrest, apoptosis, and DNA damage in Sertoli cells. • MBP impaired Sertoli cell viability and cytoskeletal organization through activation of the ATM/Chk2/p53 pathway. Dibutyl phthalate (DBP) has been recognized as an endocrine disrupting chemical, which poses significant threats to male reproductive health, though its precise mechanism of testicular toxicity has not been fully clarified. We combined in vivo and in vitro experiments with transcriptome sequencing to investigate the toxicity of DBP and its active metabolite monobutyl phthalate (MBP) in prepubertal male mice and Sertoli cells, and explored the underlying molecular mechanisms. The results showed that DBP affected testicular development and disrupted the blood-testis barrier (BTB) in mice. Through bioinformatic analysis, we validated that MBP promoted G1 phase cell cycle arrest, apoptosis, and DNA damage in Sertoli cells. Furthermore, the increased expression of p -ATM, p -Chk2, p-p53 indicated that the ATM/Chk2/p53 signaling pathway was activated upon MBP treatment. Inhibition of p53 or ATM expression partially reversed the pro-apoptotic and cell cycle-arrested phenotypes, whereas p53 overexpression reverted these phenotypic abnormalities. These findings indicated that prepubertal DBP exposure impaird BTB integrity and induced DNA damage in association with pathological activation of the ATM/Chk2/p53 signaling pathway, thereby contributing to Sertoli cell apoptosis and proliferative arrest. Download: Download high-res image (283KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Yubei Duan,Jiaxin Huang,Tianping Huang,Kaige Yang,Liwen Qi,Baohua Yu,Fanping Li,Maosheng Yin,Lei Zhao,Zhenghua Chai,Weihua Liang,Haijun Zhang,Jing Fei,Jinsong Ding,Chenghua Luo,Wenhu Zhou,Jianming Hu
HER2-positive breast cancer is characterized by aggressive proliferation, high metastatic potential, and immune evasion, yet current HER2-targeted therapies are hindered by resistance and immunosuppressive microenvironments. Here, we report a multifunctional nanoplatform (TanDNA@MnO 2 ) constructed by biomineralizing manganese dioxide with a tandem DNA (TanDNA) that integrates a HER2-targeting DNAzyme for gene silencing and a dsDNA motif for cGAS–STING activation. TanDNA@MnO 2 exhibited favorable colloidal stability, tumor accumulation, and pH/GSH-responsive Mn 2+ release. Mn 2+ served both as a cofactor to enhance DNAzyme catalytic activity and as an immune agonist to stimulate cGAS–STING signaling. Mechanistic studies revealed dual functionality: HER2 silencing directly inhibited oncogenic proliferation and relieved HER2-mediated suppression of cGAS–STING, while dsDNA and Mn 2+ synergistically amplified STING–TBK1–IRF3 activation. Consequently, TanDNA@MnO 2 remodeled the tumor immune microenvironment by promoting M1 macrophage polarization, dendritic cell maturation, and CD8 + T cell infiltration, accompanied by increased production of anti-tumor cytokines and chemokines. In vivo, TanDNA@MnO 2 achieved potent tumor growth inhibition with negligible systemic toxicity, underscoring its promise as a generalizable nucleic acid–metal oxide nanoplatform that combines gene silencing with immunotherapy to overcome resistance and immune evasion in HER2-positive breast cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Jing Zhou,MengRan Chen,Xiaonan Yang,Xianchao Yang,Kang Wang,Hang Wang,Bo Xu,Haoliang Li,Yuling Liu,Wenchang Zhang,Bing Chen,Ya Li
Optical fiber biosensors offer exceptional physical sensitivity for label-free bio-molecular detection, yet their practical performance in ultra-low concentration regimes is frequently stifled by diffusion-limited mass transport. To bridge the gap between intrinsic optical sensitivity and actual biosensing capability, an ultrasound-enhanced tapered fiber Mach−Zehnder interferometer (MZI) biosensor has been introduced, which leveraged acoustofluidic microvortices to overcome thermodynamic and kinetic barriers. A sector-shaped tip microstructure was rationally designed, which enables the generation of stable tip-coupled microbubbles within the tip chamber adjacent to the fiber surface under the actuation of a piezoelectric transducer. These bubbles can induce powerful and stable acoustic streaming vortices that actively transport analytes from the bulk solution to the sensor surface. Using ssDNA detection as the model, this active-transport mechanism lowered the limit of detection (LOD) by one order of magnitude—from 100 fM under static conditions to 10 fM under ultrasonic excitation—while boosting the overall sensitivity by 22.5%. Furthermore, the hydrodynamic shear forces generated by the microvortices provided a real-time “acoustic wash” effect, which thermodynamically destabilized non-specific binding events, thereby significantly amplifying the specificity. This work presented a generic, non-contact strategy to unlock the full potential of fiber-optic sensors, offering a robust pathway for rapid, high-fidelity clinical diagnostics.
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yuye Xuan,Yong Wei,Songquan Li,Chunlan Liu,Qian Yang,Zhihai Liu
To detect the degree of interaction between coptisine and different DNA G-quadruplexes (G4s) and screen its anti-cancer targets, this paper developed a highly sensitive fiber surface plasmon resonance (SPR) sensor using zirconium-based metal organic framework (MOF-808) nanomaterials and plastic coated multimode optical fiber, which solves the problems of background interference and low sensitivity in traditional fluorescence detection method. MOF-808 has the characteristic of high electron mobility, which increases the sensitivity of the sensor to 4869.03 nm/RIU and improves the performance by 107%. Meanwhile, thanks to the ultra-high specific surface area of MOF-808, coptisine is enriched on the sensor surface, providing more G4s binding sites, the detection limit for c-MYC Pu22 G4 is 11.15 nM. Three types of G4s were detected using the sensor, and the sensor showed a significant response to c-MYC Pu22 G4, indicating that coptisine has a strong targeting effect on Pu22 G4, which is consistent with the results of molecular docking simulation, confirming the effectiveness of the sensor as a drug target screening validation tool. This sensor exhibits good specificity, with no significant response to other substances such as double stranded DNA except for the substance being tested, and maintains excellent stability after long-term storage. This work provides a concept validation optical sensing platform for drug target screening.
✓ 已验证
产品关联
DOI
高影响因子
EBioMedicine
·
2026
·
IF 11.2
Lei Zhang,Xiaotong Han,Xinran Gao,Hangyu Chen,Chunliang Shang,Long Chen,Jian Lin,Hongyan Guo
Background Adenomyosis diagnosis and classification relies primarily on magnetic resonance imaging or pathological examination. Liquid biopsy has been widely applied in oncology but remains underexplored for adenomyosis. We aimed to identify liquid-biopsy-derived 5hmC biomarkers to aid adenomyosis diagnosis and molecular subtyping. Methods Genome-wide 5hmC profiles were obtained by 5hmC-Seal from plasma cfDNA (adenomyosis: n = 51; controls: n = 46) and tissue DNA (adenomyosis: n = 26; controls: n = 21). A paired-tissue-driven splitting strategy was used: the training set comprised 26 adenomyosis plasmas with matched tissues plus 23 controls, and the validation set comprised remaining samples. A 5hmC-based logistic regression model was developed and validated. The identified marker amyloid precursor protein ( APP ) was functionally characterised in cellular and mouse models. Findings Ten 5hmC markers were identified by machine learning. The model showed a sensitivity of 0.88 and a specificity of 0.87 (AUC = 0.91) in the validation set, with better performance than CA125 and CA199. 5hmC signatures could distinguish intrinsic from extrinsic adenomyosis subtypes. APP was related to epithelial cell migration and fibrosis in experimental models, which indicated its potential involvement in adenomyosis pathophysiology. Interpretation Plasma cfDNA 5hmC markers show promise for adenomyosis diagnosis and molecular classification. The APP-HOXD9 axis represents a potential therapeutic target warranting further validation. These findings require prospective, multi-centre confirmation before clinical implementation. Funding This work was supported by grant 82274034 from the National Natural Science Foundation of China, grant 2022YFB3604700 from the National Key Research and Development Program of China, and project BYESS2022031 from the Beijing Association for Science and Technology–Young Elite Scientist Sponsorship Program (BAST-YESS).
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Tingting Guo,Chang Zuo,Ping Bai,Lingjing Hu
A novel multi-functional DNA tetrahedron-based target-induced cascaded cyclic catalytic amplification (TICA) assay was developed for the sensitive detection of microRNA-21 (miR-21) in prostate cancer. This strategy ingeniously integrates two distinct DNA tetrahedron with specific functions (catalytic tetrahedron (Cat-DT) and signal DNA tetrahedron (Sig-DT)) to create a self-sustaining amplification circuit. The process is initiated when miR-21 triggers the release of a DNAzyme enzymatic strand (E-DNA), which converts Cat-DT into tri-branched DNA nanostructures (TB-DNA). Critically, each TB-DNA acts as a cascaded amplifier, bearing three E-DNA units that drive the exponential generation of more catalytic sites. Furthermore, the system employs an ingenious feedback loop: the TB-DNA/Sig-DT interaction produces a fluorescent signal while regenerating a miR-21-mimetic strand to perpetuate the catalytic cycle. This “target → catalyst → new target” paradigm enables ultra-efficient amplification, achieving a wide linear range from 600 fM to 100 nM and a low detection limit of 400 fM for miR-21. Notably, the TICA strategy significantly shortens the total reaction time to 80 min, demonstrating great potential for rapid and sensitive clinical diagnostics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Xinyi Zhang,Chunyu Su,Yuan Ming,Bingying Jiang,Yun Xiang,Ruo Yuan
Benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-DNA (BPDE-DNA) adduct is a critical biomarker for assessing DNA damage caused by carcinogen exposure and exhibits significant potential in lung cancer early warning. However, conventional BPDE-DNA detection methods are often constrained by limited sensitivity, complexity and high costs. This work presents the fabrication of a highly sensitive electrochemical BPDE-DNA biosensor based on a new polymerase activity inhibition strategy coupled with AuNPs/MoS 2 /Ti 3 C 2 and HRP-catalyzed signal amplifications. A three-strand DNA complex is designed to capture the target BPDE-DNA adduct by means of a toehold-mediated strand displacement reaction (TSDR) on AuNPs/MoS 2 /Ti 3 C 2 -modifed electrode. The bulky structure of the BPDE moiety subsequently introduces steric hindrance to inhibit extension of BPDE-DNA by Klenow fragment (KF) polymerase to displace the biotin-modified sequence in the three-strand complex. Streptavidin-horseradish peroxidase (STV-HRP) is then attached to electrode surface via high-affinity biotin-STV binding. The anchored HRP coupled with AuNPs/MoS 2 /Ti 3 C 2 catalyzes redox reaction of the 3,3’,5,5’-tetramethylbenzidine (TMB)/H 2 O 2 substrate solution, resulting in a substantially amplified electrochemical current for detecting BPDE-DNA in range of 0.5 pM to 10 nM with low detection limit of 0.34 pM. Our sensor methodology not only introduces a new steric hindrance strategy for BPDE-DNA detection but also offers a robust electrochemical platform for monitoring other low levels of DNA adducts.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Huiyan Cao,Xi Cheng,Li Huang,Haotian Zhang,Ziyi Liu,Wenjun Yang,Song-Mei Liu,Fang Wang
Background N6-methyladenosine (m6A), the most abundant internal modification of eukaryotic mRNA, post-transcriptionally regulates gene expression, and its dysregulation is intimately linked to tumorigenesis and multiple human diseases. However, existed detection methods including high-performance liquid chromatography (HPLC) suffer from obvious drawbacks such as site specificity constraints, high cost, reliance on sophisticated instruments and tedious operation, making them unsuitable for rapid on-site detection. Therefore, the development of a novel, rapid, on-site and cost-effective detection strategy for m6A is of great significance and urgent demand. Results This study developed an electrochemical biosensor based on DNA tetrahedrons (TDN) and gold-antibody nanoparticle (AuNPs-ab) for highly sensitive detection of m6A-RNA modifications. After enhancing conductivity through gold nanoparticle modification, TDN was stably immobilized on the electrode surface via Au-S bonds. The rigid structure of TDN was utilized to optimize surface flatness and spatial architecture, enabling precise anchoring of the antibody probe. Target m6A-RNA captured by antibodies undergoes biotin-streptavidin-mediated horseradish peroxidase (HRP) conjugation, catalyzing a hydrogen peroxide–hydroquinone (H 2 O 2 –HQ) redox cycle to generate amplified current signals. The sensor exhibits excellent linear response within the 0.001–100 nM range, and the detection limit is 0.105 pM, demonstrating high specificity and stability (signal retention rate >88% after 15 days). Furthermore, this method has been successfully applied to detect target samples spiked into human serum and total RNA extracted from hepatocellular carcinoma cells. Significance This sensing platform combines the compact size and scalable batch-production advantages of LIG electrodes, offering a novel approach for precise detection of tumor-associated m6A modifications and rational design of disposable, portable point-of-care testing (POCT) devices. It demonstrates significant potential for rapid screening of key epigenetic biomarkers and real-time monitoring across diverse clinical and biological samples.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Huibin Zhang,Haiyang Yu,Juyue Dong,Zhen Gu,Lulu Li,Kaiyutai Zhou,Wei Jiang,Wei Tang,Huifeng Wang
Triblock polyA probe enables amplification-free, label-free SGGT sensing; • Interfacial engineering optimizes probe configuration, buffer, polyA length; • LoD for synthetic L. monocytogenes DNA reaches 2.1 × 10 −17 M; • Contaminated milk samples are detected with LoD of 10 3 CFU/mL; • The sensor shows good selectivity against other foodborne pathogens. The strong signal amplification capability of solution-gated graphene transistors (SGGT) provides a promising basis for highly sensitive biosensing. However, further improvement in sensitivity is often hindered by the molecular recognition interface on the functionalized sensing electrode. In this study, an amplification-free and label-free SGGT DNA biosensor was developed by using a triblock polyadenine (polyA) DNA probe. The sensor interface was systematically optimized by capture-probe configuration, buffer composition and polyA length. Using Listeria monocytogenes ( L. monocytogenes ) as a model target, the sensor achieved limits of detection (LoD) of 2.1 × 10 −17 M for synthetic DNA fragments and 10 3 CFU/mL for L . monocytogenes in contaminated milk samples. These results demonstrate the high sensitivity and good target distinguishability in mixed samples of the sensor, addressing key limitations of conventional methods. The established sensor platform offers a new approach for achieving high-sensitivity DNA detection, with potential applications in pathogen monitoring, food safety, and molecular diagnostics. Download: Download high-res image (176KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2025
·Vol. 17
·期 25
·5298-5307
·
IF 2.6
Yang Cao,Chun Huang,Kui Li,Xi Zhang,Mengfei Tong,Jiale Tu,Kun Yang,Shuai Yuan,Hong Zhang
To sensitively detect ochratoxin A (OTA), a CRISPR/Cas12a-assisted electrochemiluminescence (ECL) aptasensor based on COF@Ru coupled with a DNA tetrahedral scaffold (DTS) was successfully fabricated. The covalent organic framework (COF) acted as a confined micro-reactor for the tris(2,2′-bipyridyl)ruthenium(II)/tri-n-propylamine (Ru(bpy)32+/TPrA) system and hence, evidently enhanced ECL signals. Au nanoparticles (AuNPs) coupled with DTS caused more ferrocene (Fc)-modified quenching DNA probe molecules to be attached to the surface of the sensing electrode, which could reduce the detection background. Activated CRISPR/Cas12a could effectively amplify the ECL signal since it could cut off a considerable amount of quenching DNA probe molecules and make them move away from the electrode. Owing to these strategies, the fabricated ECL aptasensor could detect OTA as low as 3.5 fg mL−1 in a linear detection range of 10−5–100 ng mL−1. Furthermore, the CRISPR/Cas12a and COF@Ru coupled with DTS-based ECL biosensor possessed high stability and specificity. More importantly, the ECL biosensor could effectively detect OTA in food samples, indicating that it can play a role in monitoring OTA levels in the field of food safety.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yang Jiang,Chunjia Ren,Yanxia Qiao,Shengtao Zhang,Jiayue Shi,Xia Yang,Sijia Li,Yaqian Zhou,Yan Li
Rational design of nucleic acid capture probes to modulate binding affinity is a valuable strategy for programing the dynamic range and limit of detection (LOD) in electrochemiluminescence (ECL) assays. Current strategies, however, have scarcely achieved this expectation. We present a binding affinity-driven, dynamic range, and LOD programmable triblock DNA-based dual-signal ECL platform for the nucleic acid assay. The probe architecture features a polyadenine (polyA) sequence that positions two flanking, partially complementary strands on a gold electrode with their lateral spacing precisely engineered to regulate target binding affinity. Remarkably, this system generates a dual-signal output from a single ECL luminophore─tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)32+)─where target-induced conformational changes alter the Ru(bpy)32+ distance from the electrode surface, thereby triggering distinct ECL generation mechanisms at separate electrochemical potentials. And this dual-signal output enables ratiometric or multi-target assays. This integrated platform features programmable dynamic range and LOD, single recognition element, and dual-signal output with single ECL luminophore, potentially presenting a new approach to advanced ECL nucleic acid sensing platforms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）