ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yuzhu Li,Zhongxiang Ding,Hongyan Wang,Cheng Qu,Guangping Li,Honglin Liu
In clinical diagnostics, human breath presents an alternative and more convenient sample than biofluids for detecting the ingestion of nonvolatile drugs. Surface-enhanced Raman spectroscopy (SERS) is a powerful vibrational spectroscopy technique with high sensitivity based on molecular fingerprinting. However, the low affinity of traditional SERS substrates for aerosols and the stochastic fluctuation of the SERS signal at low concentrations limit their application in breath aerosol analysis. In this study, we synthesized hydrogel microsphere SERS substrates with highly reversible swelling/shrinking properties that enhance target analyte accumulation in breath aerosols and promote plasmonic nanoparticle aggregation for intense Raman hotspot formation. Furthermore, these hydrogel microsphere SERS substrates function as a three-in-one system, enabling multilevel selectivity based on size, charge, and hydrophilicity for target molecules simultaneously without pretreatment. Notably, by “digitizing” the SERS signal of each individual hydrogel microsphere and calculating the proportion of positive microspheres, the hydrogel microspheres can serve as a digital SERS platform that circumvents the low stability issues resulting from fluctuations in SERS signal intensity. Consequently, the digital SERS platform achieved a detection limit of 0.5 ppm for fentanyl in simulated breath aerosols. This innovative sensing strategy not only demonstrates a promising approach for screening nonvolatile drugs but also simplifies the sampling process, holding great potential for clinical diagnosis of breath aerosols.
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高影响因子
NANOTECHNOLOGY
·
2024
·Vol. 35
·期 19
·195710
·
IF 2.9
Hang Chen,Caiqin Han,Le Zhang,Ying Wu
A surface enhanced Raman scattering (SERS) substrate of porous rod-shaped ferric oxide (Fe2O3) combined with silver nanoparticles (Ag NPs) and black phosphorus (Fe2O3/Ag/BP) was fabricated to detect the persistent organic pollutants (POPs) at low concentration. The organic pollutant Rhodamine 6G (R6G) was used as the probe molecule to study the performances of Fe2O3/Ag/BP, and 4-chlorobiphenyl (PCB-3) was the target of detection. The limit of detection (LOD) of R6G based on this novel SERS substrate Fe2O3/Ag/BP was as low as 1.0 × 10−15 M, which was five orders of magnitude lower than that of Fe2O3/Ag (10−10 M). The enhancement factor (EF) of Fe2O3/Ag/BP was 6.44 × 108, which was 3.1 times higher than that of porous rod-shaped Fe2O3/Ag (2.08 × 108). The Raman signal of R6G based on Fe2O3/Ag/BP had a good homogeneity, and the relative standard deviation (RSD) of Raman signal intensities of R6G at 1643 cm−1 was only 5.97%. Furthermore, the Fe2O3/Ag/BP substrate exhibited a recyclability through the photocatalytic degradation of R6G. The LOD of PCB-3 based on Fe2O3/Ag/BP was 10−9 M. Besides, Fe2O3/Ag/BP had a high SERS activity even it was kept in a centrifuge tube without requiring complicated treatment. These results highlight the potential application of Fe2O3/Ag/BP for ultra-trace detection of POPs in the environment.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Jun Feng,Yajuan Xu,Wenyi Huang,Hongxing Kong,Yanqing Li,Hao Cheng,Lijun Li
Hepatocellular carcinoma (HCC) is a common and lethal cancer. New serum markers for detecting HCC are urgently needed. Human carboxylesterase 1 (hCE1) is an important member of the serine hydrolase superfamily and is closely related to the occurrence of HCC. It can be used as a good serum marker for early diagnosis of HCC. Here, we developed a surface enhanced Raman scattering (SERS)- based magnetic immunosensor that specifically recognizes and detects trace amounts of hCE1 in human serum via a sandwich structure consisting of a SERS tags, magnetic supporting substrates, and target antigen (hCE1). The SERS tags are 4-mercaptobenzoic acid (4-MBA)-labeled AgNPs, and the SERS supporting substrates are composed of a raspberry-like morphology of Fe 3 O 4 @SiO 2 @AgNPs magnetic nanocomposites surface-functionalized with a hCE1 antibody. The prepared SERS magnetic immunosensor exhibits excellent selectivity and extremely high sensitivity for hCE1 detection. The SERS signal and logarithm of hCE1 concentration presented a wide linear response range of 0.1 ng mL −1 to 1.0 mg mL −1 , and the detection limit of hCE1 was 0.1 ng mL −1 . The results indicate that the immunosensor can be used for the rapid determination of hCE1 in human serum without a complicated sample pre-treatment. Furthermore, the immunosensor has good reproducibility and stability, and has a promising prospect for the quantitative detection of other tumor markers in early clinical diagnosis.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Wen-Shu Zhang,Ya-Ning Wang,Zhang-Run Xu
Endogenous hydrogen sulfide (H 2 S) exists in multiple physiological processes . In order to further understand the action mechanism of H 2 S in cells and human body, we proposed a smart surface-enhanced Raman scattering (SERS) nanoprobe, Au core-4-mercaptobenzonitrile-Ag shell nanoparticle ( [email protected] @Ag), for the detection of endogenous H 2 S in living cells based on the reaction between Ag shell and sulfide species. 4-MBN was selected as the SERS reporter to avoid interference from cellular molecules. With the sulfide concentration increasing, the Ag 2 S constantly formed, and consequently the SERS signal intensity of [email protected] @Ag gradually decreased owing to the weaker SERS activity of Ag 2 S. With the nanoprobes, this method not only offers a high sensitivity for H 2 S detection at an nM level, but also achieves the goal of non-background analysis. It displays satisfactory anti-interference capability and a good linear relationship with sulfide concentration ranging from 50 nM to 500 μM, and an estimated detection limit is 0.14 nM. The [email protected] @Ag nanoprobes were successfully applied to detect endogenous H 2 S in living HepG2 cells stimulated by pyridoxal 5-phosphate monohydrate. This work offers a potential analytical method in the related research of H 2 S physiological function.
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高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Xiaoxia Wu,Yuanzhi Xia,Youju Huang,Juan Li,Huimin Ruan,Tianxiang Chen,Liqiang Luo,Zheyu Shen,Aiguo Wu
Circulating tumor cells (CTCs) have received more and more attention in medical biology and clinical practice, especially diagnosis, prognosis, and cancer treatment monitoring. The detection of CTCs within the large number of healthy blood cells is a big challenge due to their rarity, which requires a detection method with supersensitivity and high specificity. In this study, we developed three kinds of new nanoparticles with the function of surface-enhanced Raman scattering (SERS) based on spherical gold nanoparticles (AuNPs), gold nanorods (AuNRs), and gold nanostars (AuNSs) with similar particle size, similar modifications, and different shapes for CTC detection without an enrichment process from the blood. The nanoparticles possess strong SERS signal due to modification of 4-mercaptobenzoic acid (4-MBA) (i.e., Raman reporter molecule), possess excellent specificity due to stabilization of reductive bovine serum albumin (rBSA) to reduce the nonspecific catching or uptake by healthy cells in blood, and possess high sensitivity due to conjugation of folic acid (FA) (i.e., a targeted ligand) to identify CTCs. Under the optimized experimental conditions, the results of detection demonstrate that these nanoparticles could all be utilized for CTC detection without enrichment process from the blood with high specificity, and the AuNS-MBA-rBSA-FA is the best one due to its supersensitivity, whose limit of detection (i.e., 1 cell/mL) is much lower than the currently reported lowest value (5 cells/mL).
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高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 30
·4630-4637
·
IF 5.047
Huijie Wang,Wenwen Ouyang,Xuerui Zhang,Jing Xue,Xiaoran Lou,Ranran Fan,Xiaonai Zhao,Lianqi Shan,Tingting Jiang
The challenge in antimicrobial photothermal therapy (PTT) is to develop strategies for decreasing the damage to cells and increasing the antibacterial efficiency. Herein, we report a novel theranostic strategy based on bacteria-induced gold nanoparticle (GNP) aggregation, in which GNPs in situ aggregated on the bacterial surface via specific targeting of vancomycin and bioorthogonal cycloaddition. Plasmonic coupling between adjacent GNPs exhibited a strong “hot spot” effect, enabling effective surface enhanced Raman scattering (SERS) imaging of bacterial pathogens. More importantly, in situ aggregation of GNPs showed strong NIR adsorption and high photothermal conversion, allowing enhanced photokilling activity against Gram-positive bacteria. In the absence of bacterial strains, GNPs were dispersed and showed a very low photothermal effect, minimizing the side effects towards surrounding healthy tissues. Given the above advantages, the bioorthogonal theranostic strategy developed in this study may find potential applications in treating bacterial infection and even multidrug-resistant bacteria.
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高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 7
·1-10
·
IF 2.101
Li Lei,Zhao Aiwu,Wang Dapeng,Guo Hongyan,Sun Henghui,He Qinye
The cube-like Fe 3 O 4 @SiO 2 @Ag (FSA) nanocomposites with great SERS activity have been successfully synthesized by a layer-by-layer procedure in this paper. The cube-like Fe 3 O 4 @SiO 2 core–shell structures were prepared via a new route and Ag nanoparticles were introduced onto their surface through a one-pot hydrothermal reaction. By controlling the reaction time, the coverage rate of Ag on the FSA surface could be tuned, and then a series of FSA composites were obtained. The SERS properties of these FSA composites were investigated using p -aminothiophenol ( p -ATP) as the probe molecule. It was found that the FSA composites synthesized with a reaction time of 6 h showed the best SERS performance, and the detection limit for p -ATP could reach 1 × 10 −7 M. For practical application, the FSA composites were also used to detect thiram, one of the dithiocarbamate fungicides that has been widely used as a pesticide in agriculture. The detection limit is as low as 1 × 10 −6 M (0.24 ppm), lower than the maximal residue limit of 7 ppm in fruit prescribed by the US Environmental Protection Agency. The resulting substrate with high SERS activity, stability and strong magnetic responsivity makes the FSA composite a perfect choice for practical SERS detection applications.
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高影响因子
Catalysts
·
2020
·Vol. 10
·期 7
·746
·
IF 3.52
Yuxiang Lu,Jikai Mao,Zelin Wang,Yazhou Qin,Jianguang Zhou
Controllable morphological metal catalytic materials have always been a focus in research. In the previous work, hexapod AgCl was successfully synthesized. In this paper, hexapod Ag@AgCl microstructures with diverse Ag contents are prepared through NaBH4reduction. They are characterized by scanning electron microscopy (SEM) and the element distribution is proved by an energy dispersive X-ray spectrometer (EDS). They are porous dendritic microstructures with a large specific surface area and a rough surface, which display high catalytic performance and surface-enhanced Raman spectroscopy (SERS) activity. Furthermore, the hexapod Ag@AgCl microstructure is devoted as a dual catalyst to monitor the reduction of 4-nitrothiophenol (4-NTP) to 4-aminothiophenol (4-ATP) in situ using SERS. Ultraviolet–visible (UV–Vis) spectroscopy was employed to evaluate the catalytic performance of the hexapod Ag@AgCl microstructures. The consequences show that the velocity of reaction became faster with a corresponding increase in silver content and the reaction mechanism was cleared. In addition to preparing a good catalyst, this work also promotes the combination of micro-nano materials and spectroscopy technology.Keywords:Ag@AgCl microstructure;in situ monitoring;surface-enhanced Raman spectroscopy;4-nitrothiophenol reduction
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Jie Yang,Ling Zhou,Xin-Yue Wang,Gao Song,Li-Jun You,Ju-Mei Li
Herein, the robust core-satellite Ag/TiO 2 /Ag composite nanospheres were successfully developed. We firstly fabricated large silver nanoparticles with sufficient plasmonic activity as cores and then encapsulated uniform titanium dioxide interlayer shell around Ag cores, which serves as the highly functionalized substrates to achieve uniform and plentiful surface chemical properties. Next, the outer shell of small silver satellites was formed in-situ via controllable reduction of AgNO 3 by n -butylamine, which acts as the remarkable enhancer for surface enhanced Raman scattering (SERS). The Ag/TiO 2 /Ag composite nanospheres with exceptional SERS sensitivity were easily prepared via facilely tuning the coverage and grain sizes of silver satellites. Then the optimized Ag/TiO 2 /Ag composite nanospheres were employed to quantitatively detect methylene blue (MB) in solution. The results showed good linear relationship between SERS intensities and logarithm concentrations of MB and the limit of detection is low to 10 −10 M. Moreover, the highly uniform and robust Ag/TiO 2 /Ag composite nanospheres display outstanding reproducibility (RSD = 4.02%) and highly storage stability. Owing to the intrinsic catalytic activity of small Ag satellites, Ag/TiO 2 /Ag nanospheres were further applied to effectively follow the degradation reaction process of crystal violet by SERS. Altogether, the core-satellite Ag/TiO 2 /Ag nanospheres present multiple SERS application potential in various fields, such as quantitative chemical analysis, trace detection of pollutants and in-situ reaction monitoring.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4751-4761
·
IF 4.157
Cai Rongyuan,Lu Dechan,She Qiutian,You Ruiyun,Feng Shangyuan,Lin Xueliang,Lu Yudong
When nitrite is ingested and absorbed by the body, it can be converted into highly toxic nitrosamines (carcinogens, teratogens, and mutagens), posing health risks to the general population. Therefore, it calls for establishing a method for determination of nitrite. In this paper, the glass-SiO 2 -Ag surface-enhanced Raman scattering (SERS) substrate with a large number of “hot spots” were prepared by two kinds of silane coupling agents. The SERS substrate had high sensitivity and repeatability. Silicon dioxide supported the silver nanoparticles (Ag NPs), which increased surface roughness of the substrate, generated a great quantity of hot spots and enhanced the SERS signal. In the SERS spectrum, the intensity ratio of the two characteristic peaks (1287 cm −1 and 1076 cm −1 ) had a good linear correlation with the logarithm of the concentration of nitrite, R 2 = 0.9652. The recoveries of 50 μM and 100 μM nitrite in three kinds of foods, three kinds of cosmetics and tap water were 90.9–105.3%. Graphical abstract
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高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Xuanyu Sha,Siqingaowa Han,Guoqiang Fang,Nan Li,Dianyang Lin,Wuliji Hasi
In this work, a facile and effective method for assembly and transfer nanoparticles on thin layer chromatography (TLC) plate was proposed and used to combined with layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS) strategy for detection of Rhodamine B and Sudan I. First, Monodispersed Ag NCs with broadly tunable diameters (from 41 to 67 nm) and 7.25 × 10 5 maximum actual enhancement factor (AEF) were successfully prepared and systematically investigated for its optic performance use in TLC-SERS. Au@Ag NCs were monitored by TEM, SEM and UV–Vis spectrometer. The optical properties of Ag NCs were simulated and the experimental results showed good consistency with simulation results of absorption spectra. Through three phases interfacial technique and a simple transfer method, nanostructures were assembled on the TLC plate. Moreover, the semi-quantitative analysis of Rhodamine B and Sudan I in the range of 1–200 ppm was performed based on the assembled TLC plate with the limit of detection as low as 1 ppm. In addition, the practical feasibility of this strategy was verified by the detection of Sudan I and Rhodamine B in chili oil. In general, this assembled TLC-SERS strategy builds a connection for the application of nanomaterials with rich morphology in TLC-SERS, which has a great application prospect and practical application value.
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高影响因子
NANOTECHNOLOGY
·
2016
·Vol. 27
·期 39
·395304
·
IF 3.573
Honghua Zhang,Dilong Liu,Lifeng Hang,Xinyang Li,Guangqiang Liu,Weiping Cai,Yue Li
A facile route has been proposed for the fabrication of morphology-controlled periodic SiO2 hierarchical micro/nanostructured arrays by reactive ion etching (RIE) using monolayer colloidal crystals as masks. By effectively controlling the experimental conditions of RIE, the morphology of a periodic SiO2 hierarchical micro/nanostructured array could be tuned from a dome-shaped one to a circular truncated cone, and finally to a circular cone. After coating a silver thin layer, these periodic micro/nanostructured arrays were used as surface-enhanced Raman scattering (SERS)-active substrates and demonstrated obvious SERS signals of 4-Aminothiophenol (4-ATP). In addition, the circular cone arrays displayed better SERS enhancement than those of the dome-shaped and circular truncated cone arrays due to the rougher surface caused by physical bombardment. After optimization of the circular cone arrays with different periodicities, an array with the periodicity of 350 nm exhibits much stronger SERS enhancement and possesses a low detection limit of 10−10 M 4-ATP. This offers a practical platform to conveniently prepare SERS-active substrates.
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