CHROMATOGRAPHIA
·
2019
·Vol. 83
·期 2
·293-297
·
IF 1.552
Zhang Pengyu,Liu Wei,Li Wei,Wang Qingshuang,Zhang Guilin,Zhao Lihui,Li Jingmei,Ji Wei,Wang Chen,Wang Jiaqi
A rapid and sensitive liquid chromatography method was developed and validated for the simultaneous determination of 21 free amino acids in nasal feeding nutrition after liquid extraction-derivatization. The analytes were extracted by PEG/EtOH (60/40, v/v), concentrated by a Dowex Monosphere 650C cation-exchange resin and derivatized using phenyl isothiocyanate (PITC). The separation was carried out on a reversed-phase Shimadzu Inertsil ODS-3 column with component A 10 mmol L −1 phosphate buffer solution (pH 6.5) and B acetonitrile/methanol/water (40/45/15, v/v/v). The optimal separation of 21 amino acids was obtained within 15 min. The average recoveries of 21 amino acids spiked at 3 concentrations (0.5, 2.0 and 10.0 µmol L −1 ) were in the range of 62.49–82.54%. Limits of detections (LODs) varied from 0.002 to 0.038 µmol L −1 and limits of quantifications (LOQs) ranged from 0.01 to 0.19 µmol L −1 . This rapid and sensitive method has been successfully applied for the analysis of nasal feeding nutrition from Chinese markets, and the contents of free amino acids in these samples are very different.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 27
·7165-7175
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IF 3.286
Zhang Meng Yuan,Huang Ren Feng,Ma Xiao Guo,Guo Li Hui,Wang Ying,Fan Yin Ming
A new fluorescence sensor (QDs-IIP), based on ion-imprinted polymers (IIP) as recognition element and Mn-doped ZnS quantum dots (QDs) as fluorophore, was synthesized for the selective and sensitive determination of hexavalent chromium (Cr(VI)) ions. The QD was first coated by SiO 2 layer, and then modified with Cr(VI)-imprinted polymer. Several parameters affecting fluorescence intensity such as tetraethylorthosilicate (TEOS) volume, ZnS:Mn@SiO 2 amount, and radiation time were investigated and optimized. The QDs-IIP was characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), transmission electron microscope (TEM), and scanning electron microscope (SEM). Furthermore, the fluorescence quenching mechanism was investigated by using UV-VIS and fluorescence spectrophotometer, and it was found that internal filtration effect was the main fluorescence quenching mechanism. The relative fluorescence intensity (F 0 /F) increased linearly with the increase of Cr(VI) concentration in the range of 20 μg L −1 –1.0 mg L −1 . The QDs-IIP sensor showed high recognition selectivity for Cr(VI) in comparison with the QDs-NIP sensor with an imprinting factor (IF) of 2.53, and it could be reused 5 times. In addition, an analytical method of Cr(VI) based on the QDs-IIP sensor was established with a limit of detection of 5.48 μg L −1 , and was then applied to actual water samples with satisfactory results. Therefore, QDs-IIP can be deemed as a practicable fluorescent sensor for trace Cr(VI) detection.
Graphical abstract
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Cellular and Molecular Bioengineering
·
2019
·Vol. 13
·期 1
·99-112
·
IF 2.477
Wang Yue,Li Xiaoli,Liu Lanlan,Liu Bingruo,Wang Feng,Chen Changsheng
Introduction Ultrasound-targeted microbubble destruction (UTMD) has been shown a promising approach for target-specific gene delivery and treatment of many diseases in the past decade. To improve the therapeutic potential of UTMD, the gene carrier of microbubbles should possess adequate DNA condensation capability and (or) specific cell or tissue selectivity. The tissue-targeted and ultrasound-targeted cationic microbubbles were developed to meet gene therapy. Methods A tissue-targeted stearic acid-inserted cationic microbubbles (SCMBs) were prepared for ultrasound-targeted gene delivery. Branched PEI was modified with stearic acid and further mixed with 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and biot-1,2-distearoyl-sn-glycero-3-phosphoethanolamine- N -[methoxy (polyethylene glycol)-2000] (ammonium salt) (Biot-DSPE-PEG2000), intercellular adhesion molecule-1 (ICAM-1) antibody and plasmid DNA to prepare cationic microbubbles through ultrasonic hydration. The ICAM-1 antibody and plasmid DNA were expected to assemble to the surface of SCMBs via biotin-avidin interaction and electrostatic interaction, respectively. Results It was found that the SCMBs had higher zeta potential compared with neutral microbubbles (NMBs) and cationic microbubbles (CMBs). In contrast, DNA incorporated SCMBs4 showed negative potential, exhibiting good DNA-binding capacity. Confocal images showed that the HeLa cells were attached around by the SCMBs4 from the view of green fluorescence of fluorescein isothiocyanate-loaded IgG which conjugated to ICAM-1 antibody on their surface. After ultrasound treatment, HeLa cells treated with SCMBs exhibited slightly stronger red fluorescence under confocal laser scanning microscope, indicating a synergistic promotion for transfection efficiency. Conclusions This tissue- and ultrasound-targeted cationic microbubble demonstrated here showed a promising strategy for improving gene therapy in the future.
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Nature Communications
·
2019
·Vol. 10
·期 1
·1-12
·
IF 11.878
Zhu Huawei,Meng Hengkai,Zhang Wei,Gao Haichun,Zhou Jie,Zhang Yanping,Li Yin
Microbial biophotovoltaics (BPV) offers a biological solution for renewable energy production by using photosynthetic microorganisms as light absorbers. Although abiotic engineering approaches, e.g., electrode modification and device optimization, can enhance the electrochemical communication between living cells and electrodes, the power densities of BPV are still low due to the weak exoelectrogenic activity of photosynthetic microorganisms. Here, we develop a BPV based on a d -lactate mediated microbial consortium consisting of photosynthetic cyanobacteria and exoelectrogenic Shewanella . By directing solar energy from photons to d -lactate, then to electricity, this BPV generates a power density of over 150 mW·m −2 in a temporal separation setup. Furthermore, a spatial-temporal separation setup with medium replenishment enables stable operation for over 40 days with an average power density of 135 mW·m −2 . These results demonstrate the electron flow constrained microbial consortium can facilitate electron export from photosynthetic cells and achieve an efficient and durable power output.
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Nature Communications
·
2019
·Vol. 10
·期 1
·1-11
·
IF 11.878
Lei Danni,He Yan-Bing,Huang Huijuan,Yuan Yifei,Zhong Guiming,Zhao Qiang,Hao Xiaoge,Zhang Danfeng,Lai Chen,Zhang Siwei,Ma Jiabin,Wei Yinping,Yu Qipeng,Lv Wei,Yu Yan,Li Baohua,Yang Quan-Hong,Yang Yong,Lu Jun,Kang Feiyu
Sodium metal batteries have potentially high energy densities, but severe sodium-dendrite growth and side reactions prevent their practical applications, especially at high temperatures. Herein, we design an inorganic ionic conductor/gel polymer electrolyte composite, where uniformly cross-linked beta alumina nanowires are compactly coated by a poly(vinylidene fluoride-co-hexafluoropropylene)-based gel polymer electrolyte through their strong molecular interactions. These beta alumina nanowires combined with the gel polymer layer create dense and homogeneous solid-liquid hybrid sodium-ion transportation channels through and along the nanowires, which promote uniform sodium deposition and formation of a stable and flat solid electrolyte interface on the sodium metal anode. Side reactions between the sodium metal and liquid electrolyte, as well as sodium dendrite formation, are successfully suppressed, especially at 60 °C. The sodium vanadium phosphate/sodium full cells with composite electrolyte exhibit 95.3% and 78.8% capacity retention after 1000 cycles at 1 C at 25 °C and 60 °C, respectively.
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JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 55
·期 4
·1727-1737
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IF 3.442
Peng Guangchun,Qiao Qianqian,Jin Lei,Zhang Bowei,Wang Yi,Huang Kang,Yao Qiong,Zhang Dongjiu,Zhang Zhan,Fang Tao,Wu Junsheng,He Yedong
A novel CeO 2 /MgAl 2 O 4 composite coating, fabricated via a cathode plasma electrolytic deposition (CPED) technique followed by hydrothermal synthesis, was developed in this study to explore its potential application as corrosion protection for AZ31 magnesium alloys. The microstructure observed through scanning electron microscopy indicated that reducing the duty cycle of the power source within a reasonable range during the CPED process was beneficial to form a uniform and dense MgAl 2 O 4 coating, which served as an ideal adhesive matrix for a uniform CeO 2 coating as the outermost layer. The results of electrochemical impedance spectra and neutral salt spray tests showed that the decoration of the CeO 2 layer significantly improved the corrosion resistance of the CeO 2 /MgAl 2 O 4 composite coating compared to the single MgAl 2 O 4 coating and bare substrate. Cross-cut tests revealed that the adhesion of the MgAl 2 O 4 coating and the CeO 2 /MgAl 2 O 4 composite coating were both excellent due to the strong binding between the MgAl 2 O 4 coating and the substrate.
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CHEMICAL PAPERS
·
2020
·Vol. 74
·期 7
·2049-2057
·
IF 1.68
Ren Peng,Li Yang,Wang Zhifen,Geng Yanxia,Yu Tao,Hua Rong
Thorium is much more abundant than uranium in nature and has become one of the important potential energy sources in the future. It is because that a thorium-232( 232 Th) nucleus has the ability to produce a uranium-233( 233 U) nucleus by absorbing a neutron. The aim of this paper is to investigate the extraction and separation of thorium ions and uranyl ions from aqueous solution with N,N’ -dimethyl- N,N’ -dioctyl-4-oxaheptanediamide (DMDOHA) into ionic liquids of 1-methyl-3-butylimid-azolium bis(trifluoromethanesulfonyl)imide ([C 4 mim][NTf 2 ]). The results show that the extractant DMDOHA has a good affinity toward thorium ions than uranyl ions in the present system, the maximum value of separation factor SF Th(IV)/U(VI) is 21.9 at pH 2.24. The extraction process of thorium ions and uranyl ions was confirmed to proceed by cation-exchange extraction mechanism. The DMDOHA extractant formed a major 3:1 complex with thorium ion and a major 2:1 complex with uranyl ion based on the relationship between log[DMDOHA] with logD.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 3
·2100-2110
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IF 2.22
Fan Lele,Liu Zhonghu,Zhu Yiyu,Wang Zhiqiang,Zhao Sihan,Zhu Lei,Zhang Qinfang
In this work, Li x BiO y compounds were synthesized via a facile calcination method. It was surprising that the as-prepared Li 7 BiO 6 compounds transformed into Li-doping tetragonal Bi 2 O 3 phase after undergoing water washing disposal, which was well verified by combining XRD and HR-TEM. In addition, the energy bandgap of prepared compound was derived based on UV–Vis diffuse reflectance spectrum and results indicate the formation of a lower band gap when increasing Li dopant concentration. Moreover, the Li-doping tetragonal Bi 2 O 3 exhibited high visible light-driven photocatalytic activity and the methylene blue can be well degraded in 10 min. The enhanced photocatalytic activity was ascribed to the improvement of light response and charge transfer. Our current work will boost the development of photocatalysis and bring insight into other Li x MO 6 (e.g., Li 6 WO 6 , Li 7 TabO 6 , Li 7 NbO 6 , Li 8 ZrO 6 ) compounds to extend their photocatalytic application.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-9
·
IF 4.011
Huang Guojie,Lin Yaqi,Zhang Linxiu,Yan Zhihong,Wang Yudong,Liu Yi
The sulfur-selenium doped carbon quantum dots (S,Se-CQDs) were synthesized by one-step through hydrothermal method in this study, which have high fluorescence quantum yield (43%) and advanced ability to scavenge reactive oxygen species (ROS). They were characterized by transmission electron microscope (TEM), nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), fourier transform infrared spectroscopy (FTIR). The results showed that the clearance rate of free radical reached to 40% with 200 μg/mL of S,Se-CQDs. The antioxidant activity of S,Se-CQDs is related to -SH and Se-SH on carbon quantum dots. S,Se-CQDs were able to access to cells which is beneficial to enhance the removal efficiency to ROS. In the biocompatibility experiment, the cell survival rate exceeded 95%, there was little effect on hatching rate, survival rate and heart rate of zebrafish which demonstrated that S,Se-CQDs have an excellent biocompatibility. It prompts that S,Se-CQDs will has proud application prospects in the field of biomedicine.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 3
·2360-2369
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IF 2.22
Yu Bin,Jia Dongling,Miao Yuqing
Herein, the Nd(OH) 3 -NiTe 2 nanoparticles were synthesized by hydrothermal synthesis. The crystal structure, morphology and surface element composition were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. At the same time, the electrocatalytic performance of Nd(OH) 3 -NiTe 2 for glucose oxidation in alkaline electrolyte was determined by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy (EIS). It exhibits a high peak current density of about 21.09 mA/cm 2 for glucose oxidation. The low semicircle diameter of 8000 Ω means fast electron transfer of Nd(OH) 3 -NiTe 2 . The oxidation signal of glucose at 0.5 V tends to be constant within 36000 s. Electrochemical experiments show that Nd(OH) 3 -NiTe 2 nanoparticles exhibit good electrocatalytic activity and stability for the oxidation of glucose, which proves their potential application in the field of fuel cells.
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JOURNAL OF ELECTRONIC MATERIALS
·
2019
·Vol. 49
·期 3
·1889-1895
·
IF 1.676
Chen Yufei,Wu Yunzhong,Dai Guoqing,Li Zhiguo,Ma Chengguo,Cui Weiwei
The silane coupling agent vinyl trimethoxysilane (a171) was used to modify graphene (GE), SiO 2 microspheres prepared by the Sol–Gel method coated on the surface of GE to obtain SiO 2 -coated modified GE (SiO 2 @a171-GE). The matrix (MBAE) was synthesized from 4,4′-diaminodiphenyl methane bismaleimide (MBMI) and diallyl bisphenol A (BBA), Bisphenol A bisallyl ether (BBE), in which BBA and BBE were used as reactive diluents. At the same time, SiO 2 @a171-GE/MBAE composites were prepared by in situ polymerization, which has SiO 2 @a171-GE as the reinforcement and MBAE as the matrix. The effect of SiO 2 @a171-GE on the MBAE material properties was studied by characterizing and analyzing the modified graphene and the coating function. The results show that GE is successfully modified by silane coupling agent, and SiO 2 microspheres are uniformly coated on the surface of a171-GE. SiO 2 @a171-GE component improves the composite properties. The dielectric constant and the dielectric loss are about 70 and 3.7 × 10 −3 in the frequency range of 10 2 –10 4 Hz, and the heat resistance enhances with the increase of SiO 2 @a171-GE. This composite has excellent dielectric, mechanical and thermal properties and has the potential to be an electrolyte material for supercapacitors.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2019
·Vol. 57
·期 3
·886-894
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IF 1.85
Xu Yujia,Dai Taotao,Huang Kechou,Liang Lu,Liu Chengmei,Chen Jun
It is an interesting topic to elucidate the interaction among plant proteins and bioactive lipid components. However, there is a shortage of understanding regarding the nature of the interaction between rice protein and conjugated linoleic acid (CLA). In this study, the intrinsic fluorescence intensity of rice glutelin (RG) was quenched upon increasing concentrations of CLA, indicating the occurrence of an interaction between them. Thermodynamic analysis showed that the RG-CLA binding process occurred spontaneously and hydrogen bonds were the primary driving force. Moreover, only one binding site was calculated between RG and CLA by the intrinsic fluorescence data. The surface hydrophobicity of RG was reduced with increasing CLA. Circular dichroism and synchronous fluorescence spectroscopy showed conformational and microenvironmental changes around the chromophores of RG. The α-helical content increased and β-sheet content declined after the binding reaction. The computational docking program displayed the target site in which CLA and amino acid residues of RG might be linked together. This study provides valuable insights into the nature of the interactions between plant proteins and fatty acids.
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