ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 24
·6197-6205
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IF 3.307
Xie Sanlei,Wen Kai,Xie Jie,Zheng Yongjun,Peng Tao,Wang Jianyi,Yao Kai,Ding Shuangyang,Jiang Haiyang
A sensitive competitive immunoassay with simple operation was developed for the detection of the anti-virus drug amantadine (AMD). The single-chain variable fragment (scFv) antibody against AMD was site-specific biotinylated and overexpressed as a secreted body in Escherichia coli AVB101. Horseradish peroxidase-labeled streptavidin-biotinylated scFv antibody (HRP-SA-BIO-scFv) could specifically bind to AMD-functionalized magnetic beads (MBs) and then the immune complexes were separated from the matrix solution by magnet. The concentration of the AMD could be known by the measurement of the signal produced by the horseradish peroxidase. The newly established assay provides a significant improvement in comparison to the conventional ELISA without SA-BIO signal amplification and MBs separation. The limit of detection and assay time was 0.64 vs. 8.4 ng/mL and 50 vs. 150 min, respectively. The recoveries ranged from 77.8 to 112% with the coefficient of variation less than 13%. The immunoassay exhibited an obvious cross-reactivity to rimantadine (84%), 1-(1-adamantyl)ethylamine (72%), and somantadine (63%). These results demonstrated that the developed immunoassay provided a sensitive, rapid, and accurate approach for the detection of AMD in chicken by employing MBs as solid phase and SA-BIO as signal amplification. When applied in natural chicken samples, the newly established method provided results consistent with those from UPLC-MS/MS, suggesting that the proposed method could be used for rapid screening of the target of interest; the new immunoassay could also be extended to other small molecular contaminants and thus represents a universal strategy for food safety analysis.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-20
·
IF 5.7
Jin Chengbo,Ma Tianle,Zhang Yiheng,Gu Xujing,Zhu Tong,Wu Xinyu,Ding Xin,Huang Suzhou,Wang Yulan,Chen Zhipeng,Fang Huihua,Wu Li
Background The progression of liver cirrhosis leads to severe complications, significantly threatening the survival and prognosis of patients. Rou gan keli (Rgkl), a herbal formula derived from classical prescriptions, has used clinically over two decades and has good efficacy. However, its molecular mechanisms and active components remain undefined. Purpose Exploring the molecular mechanisms of Rgkl in alleviating liver cirrhosis. Methods CCl 4 -induced liver cirrhosis mice models were established. Liver stiffness and intrahepatic blood flow velocity were assessed using imaging. Serum ALT, AST, HA, and histopathology were analyzed. Hepatic stellate cells (HSCs) activation, liver sinusoidal endothelial cells (LSECs) fenestration, and angiogenesis were evaluated using immunohistochemistry and scanning electron microscopy. UPLC-Q-TOF-MS/MS and network pharmacology identified active components. Transcriptomics and single-cell sequencing identified key targets and pathways, validated via WB, immunofluorescence, and molecular docking. Results Rgkl significantly reduced Liver stiffness and collagen deposition while increasing intrahepatic blood flow velocity in cirrhotic mice. Serum ALT, AST, and HA were markedly decreased. Rgkl inhibited α-SMA expression in HSC and downregulated pathological angiogenesis by reducing VEGF and CD34 expression. Additionally, Rgkl enhanced eNOS expression and preserved sinusoidal fenestration in LSEC. Furthermore, Rgkl ameliorated liver cirrhosis by modulating LSEC metabolic functions via the CD36/PPAR/CPT-1 pathway and suppressing HSC activation through the RhoA/ROCK/YAP and PI3K/AKT/NF-κB pathways. Eighteen active components, such as Levistilide A and Quercetin, were strongly correlated with the amelioration of liver cirrhosis. Conclusions Rgkl significantly attenuated hepatic injury and fibrosis. Mechanistically, Rgkl modulated LSEC lipid metabolism and phenotypic regulation, and suppressed HSC contraction and activation. Key active components contributing to these effects included Paeonilactone C, Levistilide A, and Quercetin. Graphical
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-11
·
IF 5.3
Wang Liu,Wang Mingli
A selective detection strategy is proposed for the determination of residual hypochlorite in drinking water. Flower-like Fe 3 O 4 @SiO 2 @Ag magnetic nanoparticles can aggregate on a silver film-coated magnetic substrate surface under a magnetic field, enabling the separation and enrichment of the analyte from the sample. 4-Mercaptophenol is functionalized on magnetic nanoparticles enabling quantitative detection of ClO − through a “signal off” mechanism. This substrate exhibits high sensitivity, with an analytical enhancement factor calculated using Rhodamine B (RhB) as an example reaching 1.89 × 10 5 , and a detection limit for ClO − as low as 0.0125 mg/L. The substrate also demonstrates good uniformity, reproducibility, and selectivity. Detection in actual drinking water samples indicates the potential application of this detection strategy in food safety. Graphical
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Zhang Yaohao,Yu Fei,Wang Liying,Yang Xijia,Yang Yue,Li Xuesong,Gao Yang,Jiang Yi,Jiang Ke,Lü Wei,Sun Xiaojuan,Li Dabing
To obtain higher open-circuit voltage, metal sheets and carbon electrodes are often used to construct asymmetric electrodes of water evaporation generators. However, the metal sheets hinder water evaporation, limiting device performance. Herein, an all foam-structured asymmetric water evaporation generator independent of water evaporation direction is designed. ZIF-67 has been shown to effectively enhance the surface potential of foamed iron electrodes and provide Co 2+ charge carriers for charge transfer. After combining the FI-based cathode with melamine foam and a composite carbon cloth anode to form the FI/ZIF67@CMF-MF-CC@CNTs type device, compared with the metal sheet electrode, the current density is increased by 300% up to 862 μA/cm 2 and the voltage is increased by 50% up to 782 mV. The optimal power density is 101 μW/cm 2 . We also demonstrate present device in both energy harvesting and daily electronics, highlighting its potential for scalable energy utility.
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JOM
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2025
·
IF 2.3
Zhu Yongpeng,Zhao Yuming,Liu Jinhui,Xu Xin,Sheng Kaifan,Chen Chuanyang,Li Xiaofeng,Tang Weineng,Gao Shenghui,Zhang Zexue,Gao Chunyun,Liu Qiang,Zhang Shaojun
This study investigates the chemical composition, particle size distribution, magnetic components, and alkaline sources of magnesium slag to enhance its utilization as a solid waste. Comprehensive characterization was achieved through multiple techniques, including graded sieving (16–150 mesh), magnetic separation, XRD, SEM/EDS, and EDTA titration, which systematically revealed the physicochemical properties of the slag. The sieving results indicate that β-Ca 2 SiO 4 is the dominant phase in the fraction larger than 1180 μm (11.55%), while γ-Ca 2 SiO 4 predominates in the fraction smaller than 180 μm (56.53%). The white particles in the magnesium slag originate from partially reacted dolime, whereas the black particles arise from incompletely reduced zones within the pellets. Magnetic separation analysis shows that coarse particles (> 1 mm) in the magnetic fraction mainly come from high-temperature oxidized spalls from the reduction reactor, while fine powders (< 200 μm) consist primarily of ferrosilicon (Fe x Si y ). Titration results demonstrate that the free calcium oxide (f-CaO) content accounts for 6.75 ± 0.1% by mass and serves as the primary contributor to the slag’s alkalinity. Through qualitative and quantitative analysis, this study elucidates the relationship between the physicochemical properties of magnesium slag and its resource utilization, providing key scientific evidence and practical guidance for high-value applications.
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Stem Cell Research & Therapy
·
2025
·Vol. 16
·期 1
·1-17
·
IF 7.3
Wei Kang,He Lu,Shi Yiran,Zhang Wen,Wu Meng,Zhang Shengdan,Wang Haijie,Xu Huanhuan,Hu Liangcong,Li Wei,Guo Liang
Diabetic wounds exhibit impaired angiogenesis, drug-resistant bacterial infections, and oxidative damage to the microenvironment, presenting ongoing clinical challenges. In this research, we designed a core-shell structured multifunctional microneedle (MN) array patch (CS-SDFP/HA-Ag@MOF-PAL) to expedite the healing of infected diabetic wounds. The core consists of 10,000 MV hyaluronic acid (HA) and sucrose, integrated with Ag@MOF loaded with Palmatine (Ag@MOF-PAL). The shell, composed of chitosan (CS), encapsulates SDFP (CS-SDFP). Upon application to an infected diabetic wound, the shell swells, releasing the HA core, which dissolves to release Ag@MOF-PAL. The Ag@MOF targets and eradicates bacteria and eliminates biofilms of bacteria in the wound area, such as Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa , while PAL mitigates chronic inflammation in wound macrophages via the NF-κB and MAPK signaling pathways. As the wound microenvironment normalizes, the SDFP released from the swollen CS recruits adipose-derived stem cells (ADSCs) and promotes angiogenesis to facilitate wound repair. Our experiments demonstrate that CS-SDFP/HA-Ag@MOF-PAL accelerates wound healing in a diabetic rat model with Pseudomonas aeruginosa -infected wounds. The property of this core-shell structured multifunctional MN patch presents considerable potential for the development of therapeutic strategies aimed at the healing of infected diabetic wounds.
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JOURNAL OF MATERIALS SCIENCE
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2025
·
IF 3.9
Su Yufeng,Fang Dong,Yang Hua,Liu Jian,Li Yunyan,Liu Feng
Zinc-air battery systems are considered attractive power storage technologies due to their notable theoretical energy output and low production cost. However, the slow kinetics of the cathodic oxygen reduction reaction (ORR) limits their progress toward broad commercialization. To overcome this bottleneck, we propose a novel synthetic route employing a combined microwave-ultrasound-assisted polyol reduction method, yielding a uniformly dispersed PtCo/C-MU catalyst with an average nanoparticle diameter of 3.02 nm. The synergistic integration of uniform heating afforded by microwaves and cavitation effects induced by ultrasound facilitates highly efficient co-reduction of Pt and Co precursors, driving their nucleation and growth in the polyol medium. Electrochemical evaluations demonstrate that the PtCo/C-MU catalyst delivers significantly enhanced ORR catalytic performance and superior stability in contrast to the commercial Pt/C benchmark. Notably, when integrated into zinc-air battery systems, PtCo/C-MU delivers a peak power density of 238.10 mW cm −2 and maintains operation exceeding 200 h. This readily scalable microwave-ultrasound-assisted polyol technique enables a promising route toward high-performance, economically viable catalysts. Graphical abstract
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2025
·Vol. 131
·期 11
·1-12
·
IF 2.8
Song Ziyan,Ma Ruolong,Zheng Xiaodan,Zhang Limeng,Ruan Chuanda,Yan Xiaohui,Tian Fei
In this study, Fe 3 O 4 @C@Cu 2 O was synthesized using a solvothermal method. The morphology and structural characteristics of the material were systematically investigated through various characterization techniques, including TEM, XPS, FT-IR, DLS, and VSM. Its antibacterial efficacy was assessed through colony counting. Results showed that at a concentration of 0.5 mg/mL, Fe 3 O 4 @C@Cu 2 O nanoparticles with a copper atomic percentage of 2.42% achieved bactericidal rates of 98.45% against E. coli and 90.58% against S. aureus . Compared to pure Fe 3 O 4 @C and Cu 2 O, the composite material demonstrated a 100% bactericidal rate against E. coli and 99.23% against S. aureus . Notably, after five cycles, the composite nanoparticles maintained effective bactericidal rates, with 82.16% against S. aureus and 94.72% against E. coli . SEM images of bacterial morphology and EPR tests confirmed that the antibacterial mechanism primarily involves the generation of reactive oxygen species(ROS).
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 11
·1-18
·
IF 5.3
Xie Kexin,Wang Chunfeng,Pang Ningjia,Cheng Yanan,Chen Mengyi,Mu Taotao,Xiao Deli,Di Bin
The design and synthesis of Fe 3 O 4 @PDA@poly(AA-co-EGDMA) nanoparticles (NPs) as a novel magnetic solid-phase extraction (MSPE) material for efficient enrichment and detection of synthetic cannabinoids (SCs) of various structures in large-volume wastewater is reported. The Fe 3 O 4 @PDA@poly(AA-co-EGDMA) NPs were synthesized via a two-step process involving dopamine polymerization and functionalization with poly(acrylic acid-co-ethylene glycol dimethacrylate) polymers, providing hydrophobic and acidic functional groups for enhanced analyte adsorption. An MSPE-ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and optimized for the detection of 24 SCs in wastewater. The method exhibited excellent analytical performance with good linearity ( R > 0.99), low limits of quantification (LOQ, 0.02–0.1 ng/L), and good precision with recoveries ranging from 50.20 to 92.72%. The method is capable of reliably analyzing trace SCs in large volume samples (300 mL and 1 L). This study introduces a simple, rapid, and cost-effective MSPE-UPLC-MS/MS approach for detecting SCs in complex environmental samples. The proposed method offers significant potential for wastewater-based epidemiology (WBE) applications, providing a valuable tool for monitoring drug use trends, public health surveillance, and regulatory enforcement. Graphical
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Fan Yamei,Li Rongtan,Du Xiangze,Wang Fei,Feng Xiaohui,Yang Youyuanhe,Liu Conghui,Li Jiaxin,Dong Cui,Wang Jianyang,Ta Na,Cui Wenhao,Ning Yanxiao,Mu Rentao,Fu Qiang
Hydrogen spillover has been extensively studied in heterogeneous catalysis, whereas the analogous migration of metal species remains largely underexplored. Here, we report a spillover phenomenon for metal species, exemplified by copper, which spontaneously migrates across physically contacted hydrophilic supports under humid ambient conditions. This process is facilitated by water adlayers on support surfaces, which act as molecular bridges to enable surface and interfacial migration of cooper species via hydroxylated intermediates. The phenomenon is universal across diverse supports, including oxides, carbides, and sulfides, and extends to metals such as ruthenium, cobalt, and nickel. Remarkably, catalysts prepared via this spillover approach exhibit substantially enhanced low-temperature activity in reactions including carbon monoxide oxidation, reverse water-gas shift, selective catalyst reduction with ammonia, and hydrogen cyanide oxidation, outperforming counterparts prepared by conventional impregnation. This work redefines the spillover phenomenon by extending it to metal species through water adlayer-mediated migration, opening new avenues for the design of dynamic catalysts.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-9
·
IF 15.7
Wang Ying,Zhao Ruolan,Xu Yachao,Yu Peng,Yang Shifang,Qu Yue,Feng Rongjuan,Zhang Mingyi,Liu Youxing
Uranium extraction from seawater offers a promising route to secure sustainable nuclear fuel and mitigate environmental issues from fossil fuel consumption, yet it remains hindered by low extraction efficiency and severe biofouling. Herein, we report a self-adaptative adsorbent—cyclized polyacrylonitrile–polyethyleneimine (CPAP)—which undergoes conformation expansion in alkaline seawater to enhance uranium uptake and contracts in acidic solution to facilitate uranium desorption. CPAP exhibits a high adsorption capacity of 22.3 mg g -1 , outperforming all reported fiber-based adsorbents. Its excellent photothermal effect combined with quaternary ammonium functionalities effectively suppresses biological adhesion. Mechanistic studies reveal that electrostatic repulsion and hydrogen bonding drive pH-responsive structural transitions. Furthermore, a self-designed flow-type extraction device demonstrates high uranium extraction efficiency (1.91 mg g -1 d -1 ) and excellent cycling stability, underscoring the practical scalability of this strategy. Overall, This work introduces a self-adaptative concept materials for enhancing seawater uranium harvesting, opening the door to fundamental research on designing intelligent adsorbent.
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PLANT MOLECULAR BIOLOGY
·
2025
·Vol. 115
·期 6
·1-24
·
IF 3.8
Wang Jing,Sun Shoujiang,Li Shuxia,Song Wenxue,Wang Xing,Guo Shuaiqi,Hu Xiaoya,Gao Xueqin,Fu Bingzhe
Drought is a major natural disaster that affects plant growth. Agropyron mongolicum possesses a wide range of drought tolerance genes acquired during its long evolution and adaptation to harsh environments. However, the regulatory mechanisms for drought resistance in A. mongolicum are complex, limiting the development and utilization of gene resources in response to drought stress. In this study, we examined differences in morphological, physiological, metabolite and transcript levels between the drought-tolerant (T) and drought-sensitive (S) genotypes of A. mongolicum to identify key metabolites and genes associated with the drought response. The morphological and physiological results suggest that the S genotype is suppressed by drought stress to a greater extent than the T genotype. Based on the metabolome and transcriptome data, we identified that serine/threonine-protein kinase SRK2 ( SRK2 ), peptide chain release factor subunit 1 ( eRF1 ), glutamine synthetase ( GS ), polyphenol oxidase ( PPO ), and aspartyl protease family protein ( ASP ) were highly correlated with key metabolites such as L-γ-glutamyl-L-leucine and γ-glutamylphenylalanine in leaves by co-expression network analysis, and alcohol-forming fatty acyl-CoA reductase ( FAR ), DNA oxidative demethylase ( ALKBH ), GDSL esterase/lipase ( GELP ), beta-fructofuranosidase ( INV ), and glutamine synthetase ( GS ) were highly correlated with key metabolites such as Trp-Glu-Ile and citric acid diglucoside in roots. Moreover, we identified the potential involvement of fatty acid degradation and glycolysis/glucogenesis pathways in the enhancement of drought tolerance in A. mongolicum . This study provides a foundation for genetic engineering studies of drought resistance in Poaceae plants.
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