Materials Research Letters
·
2024
·
IF 8.6
Xiaoqi Mao,Lianwen Wang,Guodong Sun,Xuezhe Zhang,Yongqing Fu,Longlong Dong
Breaking trade-off barriers of strength-ductility in Ti alloys and their composites has been a key challenge for their engineering applications. Herein, we developed a new strategy of introducing β-Ti ductile zones in heterostructured (Cu@CNTs/TC4)+TC18 composites, which were synthesized using two-stepped ball milling, spark plasma sintering and hot rolling processes. Such composites are composed of TC18-coarse grain zones (CGZ) and (TiC+α'')-reinforced TC4-fine grain zones (FGZ). They achieve an exceptional high strength and high ductility, which are superior to those of TC4 alloys and homogeneous Cu@CNTs/TC4 composites. Heterogeneous interfacial microstructures of TC4-FGZ/TC18-CGZ play an important role in strengthening and toughening effects. The grain-coordinated deformation between TC18-CG and TC4-FG is also the key reason for achieving an exceptional ductility.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Guang-Xin Mao,Jia-Min Wu,Chong Tian,Chun-Lei Liu,Xin Lin,Fen Wang,Hai-Sheng Xu,Yu-Sheng Shi
Porous Si 3 N 4 ceramics are widely applied in aerospace and mechanical fields owing to their excellent properties. Furthermore, vat photopolymerization (VPP) technology can fabricate Si 3 N 4 components with complicated structures and high precision, but its layer-by-layer printing method leads to poor mechanical properties of ceramics. In this study, porous Si 3 N 4 ceramics with a porosity of 28.41 % strengthened by directional β-Si 3 N 4 were fabricated by combining VPP technology and seeding method. Rheological behavior and curing properties of the slurry were explored, and the influence of β-Si 3 N 4 content on the mechanical properties of printed Si 3 N 4 ceramics was investigated systematically. With the increase of β-Si 3 N 4 content, the orientation degree of β-Si 3 N 4 grains increased gradually, while fracture toughness and flexural strength of the ceramics exhibited a trend of increased first and then decreased and Vickers hardness gradually decreased. As β-Si 3 N 4 content increased to 5 wt%, the fracture toughness and flexural strength of porous Si 3 N 4 ceramics were improved from 4.23 MPa m 1/2 and 214.7 MPa–5.65 MPa m 1/2 and 272.0 MPa, respectively. Therefore, this work indicates that vat photopolymerization combined with seeding method is a promising approach for the fabrication of porous Si 3 N 4 ceramics with high performance and complex structures.
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POULTRY SCIENCE
·
2024
·
IF 3.8
Xuedi Zhang,Yue Zhang,Kai Xu,Jianhua Qin,Dehe Wang,Lijun Xu,Chuanwen Wang
ABSTRACT Dermanyssus gallinae is a major hematophagous ectoparasite in layer hens. Although the acaricide β-cypermethrin has been used to control mites worldwide, D. gallinae has developed resistance to this compound. Carboxylesterases ( CarEs ) are important detoxification enzymes that confer resistance to β-cypermethrin in arthropods. However, CarEs associated with β-cypermethrin resistance in D. gallinae have not yet been functionally characterized. Here, we isolated a CarE gene ( Deg-CarE ) from D. gallinae and assayed its activity. The results revealed significantly higher expression of Deg-CarE in the β-cypermethrin-resistant strain ( RS ) than in the susceptible strain ( SS ) toward α-naphthyl acetate ( α-NA ) and β-naphthyl acetate ( β-NA ). These findings suggest that enhanced esterase activities might have contributed to β-cypermethrin resistance in D. gallinae . Quantitative real-time PCR analysis revealed that Deg-CarE expression levels were significantly higher in adults than in other life stages. Although Deg-CarE was upregulated in the RS, significant differences in gene copy numbers were not observed. Additionally, Deg-CarE expression was significantly induced by β-cypermethrin in both the SS and RS. Moreover, silencing Deg-CarE via RNA interference decreased the enzyme activity and increased the susceptibility of the RS to β-cypermethrin, confirming that Deg-CarE is crucial for β-cypermethrin detoxification. Finally, recombinant Deg-CarE ( rDeg-CarE ) expressed in Escherichia coli displayed high enzymatic activity toward α/β-NA. However, metabolic analysis indicated that rDeg-CarE did not directly metabolize β-cypermethrin. The collective findings indicate that D. gallinae resistance to β-cypermethrin is associated with elevated CarEs protein activity and increased Deg-CarE expression levels. These findings provide insights into the metabolic resistance of D. gallinae and offer scientific guidance for the management and control of D. gallinae .
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RSC Advances
·
2025
·Vol. 15
·期 11
·8346-8353
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IF 4.6
Qingshan Rong,Wei Ding,Guogang Liu,Xiangyu Fu,Yan Shi,Zhiqiang Zhang,Zhiwei Yao
In this study, we found that β-Mo2C prepared at different carbonization temperatures exhibited significantly different catalytic activities for the reverse water gas shift (RWGS) reaction. The β-Mo2C synthesized at 600 °C demonstrated notably higher activity compared to those prepared at 700 °C and 800 °C. This enhanced activity was likely attributed to its improved redox properties, which were primarily driven by a smaller crystallite size and the presence of MoxOCy species. Therefore, we proposed that the crystallite size and MoxOCy content were the key factors governing the RWGS activity of β-Mo2C. Clearly, both factors were strongly influenced by the carbonization temperature. Notably, the β-Mo2C prepared at 600 °C even outperformed Cu-doped β-Mo2C prepared at 700 °C under similar reaction conditions.
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MOLECULES
·
2025
·Vol. 30
·期 3
·685
·
IF 4.6
Lei Fang,Xun Han,Yue Zhang,Tianran Hui,Lingling Ding,Wenlu Dai,Yujie Han,Maoqiang Zheng,Guangliang Xing
Food allergies are a global concern, with β-lactoglobulin (β-LG) in bovine milk being a major allergenic protein. This study investigated the effects of transglutaminase (TGase)-mediated crosslinking on the antibody recognition capacity (ARC) and structural properties of β-LG, with the aim of developing hypoallergenic dairy products. β-LG solutions were treated with TGase at varying concentrations (0, 5, 10, 15, and 20 U/g) and durations (0, 6, 18, 24, and 42 h), followed by analysis using electrophoresis, enzyme-linked immunosorbent assay (ELISA), and spectroscopy. The results demonstrated that treatment with TGase at 20 U/g significantly reduced the ARC and immunoglobulin E (IgE) binding capacity of β-LG to 90.0 ± 0.4% and 58.4 ± 1.0%, respectively, with the optimal ARC reduction observed after 6 h of treatment (86.7 ± 1.2%,p< 0.05). Although electrophoresis did not reveal significant crosslinking of β-LG, ultraviolet absorption, fluorescence intensity, and hydrophobicity all increased with prolonged crosslinking time, while sulfhydryl content fluctuated irregularly. These findings suggest that β-LG underwent varying degrees of structural modification, which led to the masking of antigenic epitopes during the early stages (0–24 h), followed by their re-exposure at the later stage (42 h). Overall, these results highlight the potential of TGase to reduce β-LG potential allergenicity, presenting a promising strategy for the development of hypoallergenic dairy products.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinshun Ye,Xiaotong Li,Zhenzhao Weng,Yuting Li,Xiaozhen Liu,Xiangying Yu,Fengyuan Liu,Jingkun Yan,Lin Li
This study examined the effect of quinone concentration on covalent interaction between β-lactoglobulin (β-Lg) and 4-methylbenzoquinone (4MBQ). β-Lg-4MBQ-0.2, β-Lg-4MBQ-0.4, and β-Lg-4MBQ-0.8 were prepared at 1:2, 1:1, and 2:1 M ratio of 4MBQ to β-Lg thiols, respectively. β-Lg-4MBQ-0.8 had the highest polyphenol content (19.04 ± 0.17 mg/g) and the lowest free sulfhydryl (17.12 ± 0.18 μmol/g) and amino group (181.28 ± 5.37 μmol/g) contents. Compared to β-Lg, β-Lg-4MBQ conjugates showed reduced α-helix (0.82–1.26 %) and increased β-sheet (1.17–1.50 %) content. β-Lg-4MBQ-0.8 and β-Lg-4MBQ-0.4 exhibited higher surface hydrophobicity and emulsifying properties than β-Lg-4MBQ-0.2 and β-Lg. Antioxidant activity (DPPH and ABTS scavenging) followed: β-Lg-4MBQ-0.8 (46.75 ± 0.17 % and 50.97 ± 0.51 %) > β-Lg-4MBQ-0.4 (39.50 ± 0.27 % and 46.63 ± 0.59 %) > β-Lg-4MBQ-0.2 (33.35 ± 0.71 % and 43.00 ± 0.39 %) > β-Lg (31.50 ± 0.56 % and 36.25 ± 0.90 %). β-Carotene emulsions stabilized by β-Lg-4MBQ-0.4 exhibited the highest stability. These findings provide insights into developing antioxidant emulsifiers.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Weili Hao,Fanghua Yin,Ke Wang,Ling Ma,Kaoqi Lian,Hongmei Shi
Currently, antibiotic residues in the environment pose a serious public health risk; therefore, new sensitive approaches for detecting the presence of antibiotics are urgently needed. Ionic-type covalent organic frameworks (TAPB-(TFIT) + Cl − -COFs) were designed and prepared in this work, and these imine-linked COFs were characterized by several techniques, proving of porous structure and rich of nitrogen. The materials were applied to the extraction of β-lactams from aqueous samples, and the important parameters of the adsorption–desorption conditions were optimized. A dispersive solid-phase extraction method coupled with ultrahigh-performance liquid chromatography-tandem mass spectrometry (TAPB-(TFIT) + Cl − -COFs-d-SPE-UPLC-MS/MS) was proposed and utilized to determine the levels of 11 antibiotics in the β-lactams in the extracted samples. Good linearity (1.0–200.0 μg·L −1 , r > 0.9999) was achieved together with limits of detection and quantification of 0.01–0.92 μg·L −1 and 0.05–2.78 μg·L −1 , respectively; recoveries ranged from 75.57 to 114.20 %, conferring relative standard deviations < 8.99 % and negligible matrix effects (84.88–109.40 %). The synthesized TAPB-(TFIT) + Cl − -COFs were demonstrated to have good reusability and high stability. Furthermore, the proposed method was clearly shown to be sensitive and separable for the reliable simultaneous detection of 11 β-lactam residues in aquatic environments.
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Small Methods
·
2024
·
IF 10.7
Lirong He,Xingang Liu,Cheng Han,Dehui Wang,Qi Wang,Xu Deng,Chuhong Zhang
For polyvinylidene fluoride (PVDF) based piezoelectric composites, epitaxial growth of ZnO nanorods (ZnO-nr) piezoceramic layer on PVDF is an effective way to improve their piezoelectric performance. However, the crystal nucleus of ZnO featuring polar surfaces that cannot be directly attached to hydrophobic PVDF with low surface energy. Herein, direct ink writing (DIW) 3D printing is employed for the first time to create β-PVDF reservoirs with significantly enhanced surface energy, facilitating the attachment and epitaxial growth of ZnO-nr. The printed β -PVDF reservoirs designed with programmed macro-pores and abundant inner micropores, enable a higher loading of ZnO-nr by more than one magnitude, thereby boosting the electro-mechanical response. The resulting PVDF/ZnO core–shell piezoelectric energy harvester (PEH) delivers an output voltage of 33.2 V, as well as an unprecedentedly high relative output voltage of 2.76 V/wt.%, which is 2.63 times that of the state-of-the-art 3D-printed PVDF/piezoceramics PEHs. Furthermore, it can differentiate subtle human motions whereas hybrid PEHs cannot distinct. This work demonstrates that the DIW 3D printing approach offers a simple and convenient design idea for creating high performance PEHs.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2009
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IF 2.063
Dongwei Wei,Lishuo Wang,Chao Zhang
Solid−liquid equilibria have been measured for binary mixtures of 1,8-cineole with p-cymene, β-pinene, and camphene, respectively, using differential scanning calorimetry (DSC) over the whole composition concentration range. All three are simple eutectic systems. The eutectic point of the (1,8-cineole (1) + p-cymene (2)) mixture is at 200.0 K and x1 = 0.124, the (1,8-cineole (1) + β-pinene (2)) mixture at 205.6 K and x1 = 0.154, and the (1,8-cineole (1) + camphene (2)) mixture at 251.8 K and x1 = 0.582. An empirical equation of Ott and Goates was used for correlating the experimental data.
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CELLULAR SIGNALLING
·
2024
·
IF 4.4
Ziheng Yuan,Genfa Yi,Run Ma,Zhuo Wang,Jihong Hu,Wei Zhao,Ying Hu
Background Aldehyde oxidase 1 (AOX1) is associated with various pathophysiological processes, including cancer. Specifically, AOX1 has been demonstrated to have a close relationship with the progression of certain cancers. However, the expression, function, and mechanisms of action of AOX1 in gallbladder cancer (GBC) remain unclear. Methods Utilizing immunohistochemistry, the study quantified the prevalence of AOX1 within tissues of gallbladder carcinoma and those of the surrounding non-cancerous regions. In vitro assays using gallbladder carcinoma cell lines with modulated AOX1 expression levels were performed to assess the protein's role in cell proliferation, migration, and invasion. Furthermore, flow cytometry techniques were harnessed to determine the influence of AOX1 on the content of reactive oxygen species (ROS) in these cells. Additionally, the expression of epithelial-mesenchymal transition (EMT) markers and the Wnt/β-catenin signaling pathway markersin cells with varied AOX1 expression, detected through Western blot analyses. An in vivo xenograft model involving athymic mice was implemented to explore the influence of AOX1 on gallbladder tumor growth, with Western blot analysis applied to measure EMT marker expression in the resulting tumours. Results Elevated AOX1 protein levels have been observed in gallbladder carcinoma tissues, with such upregulation linked to a negative prognostic outlook for patients. In vitro analyses demonstrate that enhanced AOX1 expression facilitates gallbladder carcinoma cell proliferation, migration, and invasion, while AOX1 suppression yields an inhibitory effect on these cellular behaviors. Western blot results reveal an inverse relationship between AOX1 and E-cadherin levels, yet was positively correlation with N-cadherin, Vimentin, and Snail within both gallbladder cancer cells and in vivo xenograft tumours. Further mechanistic investigation indicates that AOX1 elevation augments reactive oxygen species (ROS) production and initiates the Wnt/β-catenin signaling pathway in these cells. The application of N -acetylcysteine (NAC) and/or KY1797K attenuates the proliferative, migratory, and invasive enhancements imparted by AOX1 overexpression and reinforces these effects when AOX1 is silenced—achieved through ROS mitigation and the obstruction of the Wnt/β-catenin pathway. In vivo studies corroborate these findings, showing AOX1 overexpression to amplify xenograft tumor growth and mesenchymal marker expression, whereas AOX1 interference did the opposite. Conclusions The study indicates that AOX1 functions as a carcinogenic factor in gallbladder carcinoma, enhancing cell proliferation, migration, invasion, and the EMT. These effects are driven by the activation of the Wnt/β-catenin pathway mediated by reactive oxygen species (ROS). Therefore,AOX1 presents potential as a valuable prognostic and diagnostic marker as well as a target for therapeutic intervention in the gallbladder cancer.
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International Journal of Nanomedicine
·
2024
·
IF 6.6
Mai Zhang,Yan Li,Chunli Han,Shiying Chu,Peng Yu,Wenbo Cheng
Background Inhibition of amyloid β protein fragment (Aβ) aggregation is considered to be one of the most effective strategies for the treatment of Alzheimer’s disease. (-)-Epigallocatechin-3-gallate (EGCG) has been found to be effective in this regard; however, owing to its low bioavailability, nanodelivery is recommended for practical applications. Compared to chemical reduction methods, biosynthesis avoids possible biotoxicity and cumbersome preparation processes.Materials and Methods The interaction between EGCG and Aβ42 was simulated by molecular docking, and green tea-conjugated gold nanoparticles (GT-Au NPs) and EGCG-Au NPs were synthesized using EGCG-enriched green tea and EGCG solutions, respectively. Surface active molecules of the particles were identified and analyzed using various liquid chromatography-tandem triple quadrupole mass spectrometry methods. ThT fluorescence assay, circular dichroism, and TEM were used to investigate the effect of synthesized particles on the inhibition of Aβ42 aggregation.Results EGCG as well as apigenin, quercetin, baicalin, and glutathione were identified as capping ligands stabilized on the surface of GT-Au NPs. They more or less inhibited Aβ42 aggregation or promoted fibril disaggregation, with EGCG being the most effective, which bound to Aβ42 through hydrogen bonding, hydrophobic interactions, etc. resulting in 39.86% and 88.50% inhibition of aggregation and disaggregation effects, respectively. EGCG-Au NPs were not as effective as free EGCG, whereas multiple thiols and polyphenols in green tea accelerated and optimized heavy metal detoxification. The synthesized GT-Au NPs conferred the efficacy of diverse ligands to the particles, with inhibition of aggregation and disaggregation effects of 54.69% and 88.75%, respectively, while increasing the yield, enhancing water solubility, and decreasing cost.Conclusion Biosynthesis of nanoparticles using green tea is a promising simple and economical drug-carrying approach to confer multiple pharmacophore molecules to Au NPs. This could be used to design new drug candidates to treat Alzheimer’s disease.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaofei Ma,Wenyi Wu,Qin Wang,Jinqiu Xu,Liangliang Cai
This study integrated a chiral selector with micelle pseudostationary phase to develop a novel chiral separation method in capillary electrophoresis. A new type of nanomicelles materials was prepared via amidation reaction using the carboxymethyl-β-cyclodextrin (CM-β-CD) as the hydrophilic group and long chain fatty amines as the hydrophobic group. This novel amide compound self-assembled into nanomicelles in a buffer solution, acting as both a chiral selector and pseudostationary phase. The separations of model drugs were notably improved when compared to the single CM-β-CD system and CM-β-CD/sodium dodecyl sulfate micellar electrokinetic capillary chromatograph system. This study optimized several separation parameters and comprehensively characterized the nanomicelles through several methods such as infrared spectroscopy, nuclear magnetic resonance spectroscopy, particle size determination, and transmission electron microscopy (TEM). Additionally, amlodipine was chosen as the model analyte to determine drug loading in both the single CM-β-CD system and the nanomicelles system using high performance liquid chromatography. The findings indicated that nanomicelles exhibited superior drug loading capacity. The particle size and TEM analysis visually confirmed the distribution of enantiomers in the hydrophobic core of nanomicelles. The proposed nanomicelles showed promise in chiral separation and this strategy opened up new pathways for the development of functional chiral materials.
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危险品化学品经营许可证（带存储）