JOURNAL OF SOLUTION CHEMISTRY
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2018
·Vol. 47
·期 12
·2068-2081
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IF 1.401
Zhang Hua-xin,Liu E.,Zhou Dan,Xia Qing-hua
Crytotanshinone (CTSO) is a Chinese herbal medicine active ingredient isolated from Salvia miltiorrhiza. In this work, the interaction of CTSO and human serum albumin (HSA) was studied by fluorescence spectra, ultraviolet spectra, circular dichroism (CD) spectra, molecular probe and molecular modeling methods. The results showed that the endogenous fluorescence of HSA was quenched by CTSO through a static mechanism. The number of binding sites, equilibrium constants, and thermodynamic parameters of the reaction were calculated at three different temperatures. The positive enthalpy change (Δ H θ ) and entropy change (Δ S θ ) revealed that the interaction was an endothermic as well as an entropy-driven process, where hydrophobic power played the major role in stabilizing the structure of the new complex. Site-selective binding experiments were carried out using warfarin and ibuprofen as probes, which proved that CTSO binds to Sudlow’s site II in subdomain IIIA of the HSA molecule. Circular dichroism (CD) spectra was employed to detect the α -helix and β -strand contents in HSA before and after the binding of CTSO. Based on the experimental results, the structure of the CTSO–HSA complex was calculated by docking CTSO to the proven site using molecular modeling. The study obtained comprehensive information on structure and thermodynamics, which is essential for understanding the bioaffinity, delivery process and pharmacological mechanism.
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Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
·
IF 4.122
Chen Yanmei,Shang Xuefang,Li Congshu,Xue Zhenzhen,Chen Hongli,Wu Hongwei,Wang Tianyun
A series of chemsensors ( 1 – 4 ) containing fluorobenzene group based on coumarin derivatives have been developed for the selective and sensitive detection of H 2 S. The advantages of the synthesized fluorescent probe (compound 1 ) were the low detection limit (4 × 10 −6 mol·L −1 ), good selectivity and high sensitivity which had been demonstrated through UV-vis, fluorescent titration experiments. Besides cytotoxicity test of compounds ( 1 and 2 ) was studied and the results indicated that compounds ( 1 and 2 ) showed almost no cytotoxicityat at a concentration of 150 μg·mL −1 . The interacted mechanism was the thiolysis reaction of dinitrophenyl ether which had been confirmed by fluorescence and HRMS titration experiment. In addition, probe 1 can also detect HS − selectively by naked eye in pure DMSO solvent.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 23
·5963-5973
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IF 3.286
Xiong Jie,Yang Lu,Gao Li Xia,Zhu Pan Pan,Chen Qian,Tan Ke Jun
A microscale highly fluorescent Eu metal-organic framework (Eu-MOF) was synthesized with terephthalic acid and 1H-1,2,4-triazole-3,5-diamine by one-pot hydrothermal method. And it was characterized by scanning electron microscope, Fourier transform infrared spectroscopy, powder X-ray diffraction, fluorescence spectroscopy, thermogravimetric analysis, and energy dispersive X-ray mapping. The prepared Eu-MOF has high quantum yield of 30.99%, excellent water dispersibility, good fluorescence stability, and favorable thermal stability. Based on the distinctly different fluorescence responses of different emission, the prepared Eu-MOF was used as dual-mode visual sensor for the sensitive detection of berberine hydrochloride and tetracycline. The limits of detection are 78 nM and 17 nM, respectively. The sensing mechanism was also discussed. Moreover, a filter paper sensor has been designed for sensing tetracycline with a notable fluorescence color change from blue to red. The prepared Eu-MOF is promising to be developed as a multi-mode luminescent sensor for visual detection in biochemical analysis.
Illustration of the synthesis of Eu-MOF and its sensing applications for berberine hydrochloride and tetracycline
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 21
·5437-5444
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IF 3.286
Jiao Xiangyu,Zhou Yabin,Zhao Dan,Pang Duo,Wang Changtao,Du Hongwu,Wen Yongqiang,Zhang Xueji
To meet the need for high-throughput immunoassays, many multiplex fluorescent immunoassays have been proposed. Most of them need different kinds of fluorescent label indicators during the test. In this work, a novel indirect ELISA-inspired dual-channel fluorescent immunoassay based on 3-mercaptopropionic acid capped CdTe/ZnS quantum dots (QDs) was constructed. The ELISA wells were coated with two kinds of antigen–QD complex. When the primary antibodies were present in a sample, they mediated the binding of a secondary antibody–DNA–gold nanoparticle complex to the antigen–QD complex. Then the gold nanoparticles quenched the fluorescence of the QDs and a decrease in fluorescence intensity was observed. Thus, the amount of primary antibody could be estimated from the decrease of fluorescence intensity. Owing to the wide absorption range and the relatively narrow emission band of the QDs, the dual-channel fluorescent immunoassay system could work at the same excitation wavelength and the emission wavelengths of each channel had no interference. As a result, two different kinds of primary antibody could be detected at the same time in one ELISA well, which simplified the operation and greatly improved the efficiency. Besides, only one type of secondary antibody needs to be added to the prepared microtiter plates, which further simplified the operation during the detection procedure. This dual-channel fluorescent immunoassay system will provide new insights into high-throughput immunodetection.
Graphical abstract
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JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 20
·13135-13146
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IF 3.442
Dong Peiran,Long Chunguang,Peng Ying,Peng Xin,Guo Fangyu
The purpose of this work is to solve the problem of interface bonding in aluminum foam (AF)/polyoxymethylene (POM) interpenetrating phase composites (AF/POM). Silane coupling agent (KH-550), graphene oxide (GO), sulfonated graphene (S-GNS) and graphene (GS) coatings were prepared on the surface of AF, respectively, as an interfacial transition layer. The molten modified POM(POM C ) was injected into the hole of coated AF by injection molding to prepare different kinds of coated AF/POM C . Thermal conductivity, friction coefficient and wear loss of coated AF/POM C were analyzed, and the wear mechanisms were discussed in detail based on evidences from SEM. The effect of coatings on thermal conductivity of coated AF/POM C was also verified by ANSYS. The investigation has shown that four kinds of coating are successfully covered on the surface of AF, respectively, and the morphology of S-GNS coating is the smoothest. The thermal conductivity of coated AF/POM C is higher than that of AF/POM C . The thermal conductivity of S-GNS-AF/POM C reaches the highest value of 2.25 W m −1 K −1 , because S-GNS coating plays a role in reducing the thermal resistance of the interface between AF and POM C phases. The friction coefficient and wear loss of coated AF/POM C are also lower than that of AF/POM C . Three types of graphene coating(S-GNS,GO,GS) as an interface transition layer between AF and POM C phases can homogenize friction, accelerate heat transfer and reduce the friction coefficient and wear loss of composites. Through ANSYS thermal steady-state simulation, S-GNS-AF/POM C has the maximum number of heat flow paths and the highest heat flux compared with other materials.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2019
·Vol. 193
·期 2
·422-433
·
IF 2.431
Hu Peilin,Du Jie,Zhang Shuai,Wang Ting,Li Jun,Chen Gang,Zhou Guangqian
Osteoarthritis (OA), a common degenerative disease affecting articular cartilage, is caused by multiple factors, and currently, there are few approaches to effectively delay its progression. This study aimed to evaluate whether a strontium compound (in the form of strontium gluconate, Glu-Sr) could reduce OA pathology severity in osteoarthritic rat models by directly targeting chondrocytes, including catabolic/anabolic activities and/or chondrogenic differentiation. Glu-Sr was administered to OA rats by oral gavage beginning during OA induction and continuing for 8 weeks. Glu-Sr treatment was found to significantly reduce cartilage degeneration and delay OA progression. Further examination showed that collagen II, Sox9, and aggrecan (ACAN) genes were up-regulated whereas IL-1β was down-regulated in chondrocytes isolated from Glu-Sr-treated rats. Glu-Sr also antagonized the catabolic effects of IL-1β on chondrocytes. Furthermore, Glu-Sr was shown to promote the chondrogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs), possibly through promoting chondrogenic gene expression, including CTGF and FGF1, as revealed by RNA-sequencing (RNA-seq). These results suggest that systemic administration of Glu-Sr may be useful in prophylactic and therapeutic treatment of chronic cartilage degradation through affecting multiple steps from chondrogenic differentiation of progenitors to matrix formation in mature chondrocytes.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-8
·
IF 5.479
Fan Xuexue,Xu Yanan,Sheng Tiandu,Zhao Dongqing,Yuan Haikuan,Liu Fengjiao,Liu Xijian,Zhu Xueyan,Zhang Lijuan,Lu Jie
A surface-graphenized pencil graphite electrode (SGPGE) served as an amperometric sensor for dopamine (DA). It was prepared through a one-step in-situ electrochemical graphene delamination. The graphite particles on the outer surface of the pencil graphite electrode (PGE) were delaminated by controlling the electrochemical delaminating conditions such as the applied anodic voltage and polarization duration, as well as the kind of electrolytes. The best conditions were identified by scanning electron microscopy, Raman spectra, cyclic voltammetry and differential pulse voltammetry (DPV). As a result, the electrode was endowed with an optimum combination of graphene delamination efficiency and electrochemical activity. The electrochemical treatment activates the surface sensing sites and improves the sensing performance. The NaOH-teated anodically graphenized electrode was used to sense dopamine by DPV. The best oxidation voltage of dopamine is at around 0.17 V (vs. SCE). The electrode respondsy to dopamine in the ranges of 0.15 to 45 μM, the detection limit is 8.2 nM (S/ N = 3), and the sensitivity is 20.81 μA μM −1 cm −2 . In real human urine samples, the sensor exhibited detection recoveries of 97.4–98.8% and low relative standard deviations of 3.49–3.92%.
Schematic presentation of a surface-graphenized pencil graphite electrode (SGPGE) for detecting dopamine. It was prepared by a one-step in situ electrochemical graphene delamination.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 30
·期 3
·2501-2511
·
IF 2.324
Zhang Zhao,Yang Hui,Wang Hao,Ding Xingeng,Zhang Qilong,Zhu Zhicai
In this work, KTa 0.2 Nb 0.8 O 3 –BaTiO 3 (KTN–BT) hybrid nanoparticles were synthesized via a facile one-pot hydrothermal method. Flexible nanocomposite films comprised of poly (vinylidene fluoride-trifluoroethylene-chlorotrifluoroethylene) P(VDF-TrFE-CTFE) matrix and KTN–BT hybrid fillers of different molar ratio were prepared by solution-casting. It is revealed that the introduction of BT to the KTN causes decreased grain size and homogenous morphology, improving the dielectric constant, breakdown strengthen and energy density of nanocomposite films. Typically, the nanocomposite film containing 40 vol% KTN–BT possesses a dielectric constant of 322 at 100 Hz, which is 8.7 times than that of pure polymer. Also, nanocomposite film with < 10 vol% of filler could achieve high breakdown strength of over 300 MV m −1 . The energy density of the film containing 2 vol% KTN–BT filler is 61.4% higher than that of pure P(VDF-TrFE-CTFE) polymer. (7.1 J cm −3 compared to 4.4 J cm −3 .) In addition, the nanocomposites also displayed good flexibility and kept excellent dielectric properties after bending and folding. All the improved performance enables these composites to meet the requirements of many flexible electronic devices and energy storage devices.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 30
·期 2
·1487-1495
·
IF 2.324
Liu Yangwen,Gao Fengmei,Wang Lin,Yang Weiyou,He Xinbo,Hou Huilin
In the present work, we reported the fabrication of CdS decorated mesoporous SiC hollow nanofibers for efficient visible-light-driven photocatalytic hydrogen production. The mesoporous SiC hollow nanofibers were firstly fabricated by electrospinning, followed by hydrothermal treatment to introduce the CdS nanoparticles decorated on the preformed fiber matrix, The resultant CdS/SiC hybrid photocatalysts were systematically characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM) and N 2 adsorption. The as-fabricated CdS/SiC hybrids exhibited a robust stability with a release rate up to ~ 124.65 µmol h −1 g −1 for photocatalytic H 2 evolution driven by visible light, which was profoundly enhanced for more than 16 times to that of pristine SiC counterparts, and comparable to the state-of-the-art one of SiC-based photocatalysts.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 11
·1-9
·
IF 5.705
Kang Yan,Wu Ting,Han Xiaozhen,Gu Haixin,Zhang Xin
A micro surface-enhanced Raman scattering (SERS) substrate has been fabricated by electrochemical deposition of dendrite-like gold on carbon fiber needles (Au-CFNs). Scanning electron microscopy and energy dispersive spectroscopy were used to confirm the presence of the gold nanostructure on the CFNs. This substrate has a Raman scattering enhancement factor as high as 3.3 × 10^7 when using rhodamine 6G as the reporter molecule. The high SERS sensitivity is attributed to the massive hotspots on gold bulges that enhance the local surface plasmon resonance. The Au-CFN substrate was reproduced 10 times after electrochemically wiping off the analytes from the needle-like electrode. The substrate has attractive features such as convenient sampling, low sample dosage, and minimal invasion. It was applied, in combination with thin-layer chromatography, for the determination of acetamiprid on vegetables. The result was more accurate because the sample information of both the surface and the bulk can be obtained at the same time after inserting the tip of this needle substrate into the TLC plate. The limit of detection for acetamiprid is 0.05 μg⋅mL -1 and the linear range is 0.1–10 μg⋅mL -1 .
A reusable micro needle-like SERS substrate was fabricated and applied for pesticide residue analysis. The SERS signal of acetamiprid can be acquired on the tip of this micro needle-like Au-CFN substrate. This substrate can be reused for 10 times.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 29
·7783-7792
·
IF 3.307
Zheng Hong,Ni Zhitao,Cai Aimin,Zhang Xi,Chen Jiuxia,Shu Qi,Gao Hongchang
Untargeted metabolomics attempts to acquire a comprehensive and reproducible set of small-molecule metabolites in biological systems. However, metabolite extraction method significantly affects the quality of metabolomics data. In the present study, we calculated the number of peaks (NP) and coefficient of variation (CV) to reflect metabolome coverage and reproducibility in untargeted NMR-based metabolic profiling of tissue samples in rats under different methanol/chloroform/water (MCW) extraction conditions. Different MCW extractions expectedly generated diverse characteristics of metabolome. Moreover, the classic MCW method revealed tissue-specific differences in the NP and CV values. To obtain high-quality metabolomics data, therefore, we used mixture design methods to optimize the MCW extraction strategy by maximizing the NP value and minimizing the CV value in each tissue sample. Results show that the optimal formulations of MCW extraction were 2:2:8 (ml/mg tissue) for brain sample, 2:4:6 (ml/mg tissue) for heart sample, 1.3:2:8.7 (ml/mg tissue) for liver sample, 4:2:6 (ml/mg tissue) for kidney sample, 2:3:7 (ml/mg tissue) for muscle sample, and 2:4:6 (ml/mg tissue) for pancreas sample. Therefore, these findings demonstrate that different tissue samples need a specific optimal extraction condition for balancing metabolome coverage and reproducibility in the untargeted metabolomics study. Mixture design method is an effective tool to optimize metabolite extraction strategy for tissue samples.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 4
·3319-3330
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IF 2.993
Sun Ning,Lei Rong,Xu Jianghui,Kundu Subhas C.,Cai Yurong,Yao Juming,Ni Qingqing
To improve the roles of tumor drug, porous silk fibroin particles have been designed as drug carriers. Doxorubicin (DOX) is encapsulated during the fabrication process of the silk fibroin particles to form DOX loaded silk fibroin particles (SFPs-DOX). Folic acid (FA) is covalently grafted to the surface of the silk fibroin particles (FA-SFPs-DOX) as a target group to the folate receptor of tumor cells.
The experimental results showed that under the conditions of low pH, high ionic strength and high enzyme concentration, the release of loaded drugs in particles has stimulus response under various conditions. The synthesized silk fibroin particles have good biocompatibility. The specific binding of fibroin particles to folate receptors provides the particles to with target functions to the tumor cells. The job develops potential application of silk fibroin particles as smart drug carriers for the targeted therapy of tumor.
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