IONICS
·
2022
·Vol. 28
·期 10
·4825-4832
·
IF 2.961
Yang Lu,Zhang Yu,He Zhiyuan,Tang Xin,Dong Qiaoyan,Tang Jiayuan,Huang Ke,Zou Zhirong,Yu Huimin,Xiong Xiaoli
Cobalt-based catalysts are widely used as non-enzymatic electrochemical sensors due to their high natural abundance, low-cost, tunable valence states, and high electron transfer efficiency. In this work, carbon matrix-embedded cobalt metaphosphate nanosheets (Co 2 P 4 O 12 -C/CC) was successfully prepared by a phosphorylation process of cobalt zeolitic imidazolate frameworks (Co-ZIF) grown on carbon cloth (CC). Benefiting from the many gaps between the carbon matrix-embedded nanosheet arrays, the specific surface area and active sites of the catalyst electrode and its stability are significantly increased. As a working electrode, a non-enzymatic electrochemical sensor for glucose was constructed. The sensor exhibits good sensitivity ( 2092 µA mM −1 cm −2 ) with linear range of 1.0 µM to 2.0 mM and detection limit of 0.12 µM (S/N = 3). Further analysis of human serum obtained satisfactory results. This work opens a new door for the construction and application of cobalt-based non-enzymatic electrochemical sensors.
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FEMS MICROBIOLOGY ECOLOGY
·
2014
·Vol. 88
·期 1
·107-120
·
IF 3.875
Zhou Shungui,Xu Jielong,Yang Guiqin,Zhuang Li
Iron oxides and humic substances (humics) have substantial effects on biochemical processes, such as methanogenesis, due to their redox reactivity and ubiquitous presence. This study aimed to investigate how methanogenesis is affected by the common occurrence of these compounds, which has not been considered to date. The experiment was conducted with anoxic paddy soil microcosms receiving a humics surrogate compound (anthraquinone-2,6-disulfonate, AQDS) and three iron(III) oxides (ferrihydrite, hematite, and magnetite) differing in crystallinity and conductivity. Ferrihydrite suppressed methanogenesis, whereas AQDS, hematite, and magnetite facilitated methanogenesis. CH4 production in co-occurring ferrihydrite + AQDS, hematite + AQDS, and magnetite + AQDS cultures was 4.1, 1.3, and 0.9 times greater than the corresponding cultures without AQDS, respectively. Syntrophic cooperation between Geobacter and Methanosarcina occurred in the methanogenesis-facilitated cultures. Experimental results suggested that the conductive characteristics of iron(III) oxides was an important factor determining the methanogenic response to the co-occurrence of iron(III) oxides and humics in anaerobic paddy soil. This work indicated that the type of iron(III) oxides may significantly affect carbon cycling under anoxic conditions in natural wetlands.
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MACROMOLECULAR BIOSCIENCE
·
2020
·Vol. 20
·期 3
·1900395
·
IF 3.416
Kun Yao,Wei Li,Kaiyan Li,Qirui Wu,Yarong Gu,Lijuan Zhao,Yuan Zhang,Xinghua Gao
Microfluidic spinning, as a combination of wet spinning and microfluidic technology, has been used to develop microfibers with special structures to facilitate cell 3D culture/co-culture and microtissue formation in vitro. In this study, a simple microchip-based microfluidic spinning strategy is presented for the fabrication of multicomponent heterogeneous calcium alginate microfibers. The use of two kinds of microchip enables the one-step preparation of multicomponent heterogeneous microfibers with various arrangement patterns, including the preparation of one-, two-, and three-component microfibers by a two-layer microchip and preparation of four component microfibers with different arrangement by a membrane-sandwiched three-layer microchip. The obtained microfibers could be used to encapsulate various kinds of cells, such as the human non-small cell lung cancer cell NCI-H1650, the human fetal lung fibroblast HFL1, the normal pulmonary bronchial epithelial cell 16HBE, and human umbilical vein endothelial cells. By adding chitosan to the medium to keep the fibers stable, 3D long-term in vitro cell co-culture has been carried out up to 21 days. This method is very simple and easy to operate, continuously produces spatially well-defined heterogeneous microfibers, has important applications for composite functional biomaterials, and shows great potential in organs-on-a-chip and biomimetic systems.
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ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Wing-Fu Lai,Andrei S. Susha,Andrey L. Rogach
Multidrug therapy may yield higher therapeutic effects as compared to monotherapy, yet its wide application has been hampered by the limitations of conventional drug delivery systems, in which not only incompatible drugs cannot be co-delivered but also the release rates of individual co-delivered drugs cannot be tuned separately. Regarding these limitations, we adopt the microfluidic electrospray technology to fabricate alginate-based multicompartment microgel beads. By using cadmium–telluride (CdTe) quantum dots (QDs) and a quenching agent as a model pair, the beads are shown to effectively separate incompatible drugs during co-delivery, and significantly prolong the time of observable fluorescence emission from QDs co-delivered with a quenching agent. Moreover, the drug release rates from different compartments can be tuned using the polymer blending technique to achieve a variety of drug release patterns. This study is one of the first to adopt the microfluidic electrospray technology to generate microgel beads with such versatility for co-delivery of multiple drugs. Our results provide evidence for the promising potential of our beads to be further developed as a carrier for multidrug therapy and other applications that require co-administration of multiple bioactive agents.
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Food Bioscience
·
2022
·
IF 5.318
Yao Liu,Songyi Lin,Shengjie Hu,Di Wang,Hui Yao,Na Sun
A specific peptide (EEEFDATR) that is released during the hydrolysis of Antarctic krill contains three glutamic acids that cover calcium-binding ligands, and might act as a potential role in enhancing the osteogenetic activity. In this study, EEEFDATR and calcium alone and in combination were examined for their effects on the proliferation, differentiation, and mineralization of MC3T3-E1 osteoblastic cells. Co-administration of CaCl 2 and EEEFDATR showed a superior proliferation rate (70.94%) of MC3T3-E1 cells, which was about 2.07 and 1.74 times higher than CaCl 2 and EEEFDATR alone, respectively. Osteoblast proliferation improvements were associated with increased cell cycle in S phase after co-administration of CaCl 2 and EEEFDATR. The CaCl 2 + EEEFDATR group further exhibited a significantly higher alkaline phosphatase (ALP) activity as compared to other groups ( p < 0.05) and showed obvious differentiation of MC3T3-E1 cells. The magnitude of mineralization in the extracellular matrix (calcium deposition) was also higher in MC3T3-E1 cells treated with combined administration, although EEEFDATR alone did not induce significant mineralization. These data suggest that co-administration of CaCl 2 and EEEFDATR was superior to mono-administration in improving osteogenetic activity.
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ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 6
·1453-1460
·
IF 6.15
Wang Yu-zhe,Yang De-hua,Wang Ming-wei
Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are regarded as ‘incretins’ working closely to regulate glucose homeostasis. Unimolecular dual and triple agonists of GLP-1R and GIPR have shown remarkable clinical benefits in treating type 2 diabetes. However, their pharmacological characterization is usually carried out in a single receptor-expressing system. In the present study we constructed a co-expression system of both GLP-1R and GIPR to study the signaling profiles elicited by mono, dual and triple agonists. We show that when the two receptors were co-expressed in HEK 293T cells with comparable receptor ratio to pancreatic cancer cells, GIP predominately induced cAMP accumulation while GLP-1 was biased towards β-arrestin 2 recruitment. The presence of GIPR negatively impacted GLP-1R-mediated cAMP and β-arrestin 2 responses. While sharing some common modulating features, dual agonists (peptide 19 and LY3298176) and a triple agonist displayed differentiated signaling profiles as well as negative impact on the heteromerization that may help interpret their superior clinical efficacies.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Yue Yu,Yimin Sun,Xiong Zhou,Yurong Mao,Yunxiu Liu,Ling Ye,Li Kuang,Jing Yang,Yi Deng
Polyetheretherketone (PEEK) has been well concerned as a promising material for hard tissue repair because of its outstanding mechanical behavior and superior biocompatibility. However, its clinical application is limited by its biological inertness and the susceptibility to bacterial infection during implantation. To improve the original shortcomings, self-polymerized dopamine (PDA) was used to enrich silver ions on the PEEK surface. Moreover, a layer of carboxymethyl chitosan (CMC) film was formed on the PEEK surface by the spin-coating method, aiming to control the release of silver ions on the surface. At the same time, bone forming peptide (BFP) was modified onto the PEEK surface by 1-(3-dimethylaminopropyl)-3-ethylcarbonimide hydrochloride (EDC) / N-hydroxy succinimide (NHS). The characterization results showed that PEEK-Ag-CMC-BFP could be obtained successfully. The inhibition zone and bacterial kinetic curve showed a favorable inhibitory effect of the sliver-modified PEEK on gram-negative and gram-positive bacteria. In vitro experiments exhibited that PEEK-Ag-CMC-BFP had a better biological activity than that of PEEK, which could promote cell proliferation and osteogenic differentiation. It is expected that this dual-function material with antibacterial and bone-promoting properties has a vast potential applied in the field of hard tissue repair.
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JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 10
·1-9
·
IF 3.061
Li Chen,Long Xin,Wang Qingyin,Li Jianguo,Zhang Hua,Wang Gongying
Copolycarbonate was synthesized by melt transesterification method, using diphenyl carbonate, isosorbide and 1,4-cyclohexanedimethanol (CHDM) as raw materials. The copolymer composition and random structure of the copolymer were determined by Fourier transform infrared and 1 H NMR analysis. The DSC and TGA analysis showed that CHDM was introduced into the isosorbide polycarbonate chain, which effectively lowered the glass transition temperature of the polymer without lowering the thermal decomposition temperature. The glass transition temperature of the copolymer can be controlled by the addition amount of CHDM, and its processing performance can be improved. In addition, the introduction of CHDM did not reduce the high light transmittance (> 91.5%) of isosorbide polycarbonate. With the gradual increase of the amount of CHDM added, the refractive index of the copolymer decreases slightly. When the CHDM content is 30 mol%, the glass transition temperature (Tg)of the copolymer is 132.2 °C, the thermal decomposition temperature is > 350 °C, the refractive index is 1.4960, the Abbe number is 51, and the transmittance is 91.6%, and the haze is 1.07%.
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CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Zhiman Yang,Xiaoshuang Shi,Meng Dai,Lin Wang,Xiaohui Xu,Rongbo Guo
Potential of effluents from biological hydrogen production process to improve ciprofloxacin (CIP) removal was investigated using ciprofloxacin-degrading enrichments in the presence of Fe 3 O 4 nanoparticles . Effluent addition clearly promoted CIP removal, which was 61%, 38% and 16% higher than those in the enrichments amended with fecal sewage , biogas digestate and water, respectively. Experiments of sterilization and organic components of effluents indicated that the organic substances served as co-metabolic substrate to facilitate CIP removal, where ethanol was the preferred co-metabolic substrate. Although the effluent addition changed the bacterial community composition which corresponded to the changes in the relative abundances of dominant genera in the enrichments, CIP removal performance remained unchanged. The increased Fe (II) concentrations indicated that there might be a positive relationship of CIP removal with the microbial reduction of Fe (III) to Fe (II) in Fe 3 O 4 in the effluents-amended enrichments. It was proposed that the organic substances in the effluents might serve as the co-metabolic substrate to promote CIP removal by CIP-degrading and Fe (III)-reducing bacteria.
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MOLECULAR PHARMACEUTICS
·
2018
·
IF 4.556
Yijia Yin,Qian Hu,Chenfeng Xu,Qi Qiao,Xianya Qin,Qingle Song,Yang Peng,Yongdan Zhao,Zhiping Zhang
A dual-sensitive nanoparticle delivery system was constructed by incorporating an acid sensitive hydrazone linker into thermosensitive nanoparticles (TSNs) for co-encapsulating doxorubicin (DOX) and interferon γ (IFNγ) and to realize the co-delivery of chemotherapy and immunotherapy agents against melanoma. DOX, a chemotherapeutic drug, was conjugated to TSNs by a pH-sensitive chemical bond, and IFNγ, a potent immune-modulator, was absorbed into TSNs through the thermosensitivity and electrostatics of nanoparticles. Consequently, the dual sensitive drug-loaded TSN delivery systems were successfully built and showed an obvious core–shell structure, good encapsulation efficiency of drugs, sustained and sensitive drug release, prolonged circulation time, as well as excellent synergistic antitumor efficiency against B16F10 tumor bearing mice. Moreover, the combinational antitumor immune responses of hydrazone bearing DOX/IFNγ-TSN (hyd) were strengthened by activating Th1-type CD4+ T cells, cytotoxic T lymphocytes, and natural killer cells, downregulating the expression levels of immunosuppressive cytokines, such as IL10 and TGFβ, and upregulating the secretion of IL2 and TNFα. Taken together, the multifunctional TSNs system provides a promising strategy for multiple drugs co-delivery with distinct properties.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Wang Yin,Liu Tian,Shenchun Wang,Dezhen Zhang,Shengrong Guo,Meidong Lang
This study reported a co-delivery system of paclitaxel (PTX) and doxorubicin (DOX) based on a functionalized poly(ϵ-caprolactone) (FC) conjugated with folate (FA) and PTX prodrug (FA-FC-PTX). PTX was conjugated to pendant carboxyl groups of FC by esterification, and FA was modified to PEG block of FC. The release behaviors of DOX and PTX from the micelles of FA-FC-PTX loaded with DOX (FA-FC-PTX/DOX) were obviously different. DOX was almost completely released in the initial 24 h, while a small amount of PTX was only released for 24 h, and continued to be released for 7 days. The cellular uptake results showed that FA-FC-PTX/DOX could be more effectively internalized by 4T1 cell than the non-folate drug-loaded micelles. In vitro cytotoxicity assays demonstrated that FA-FC-PTX/DOX revealed synergistic effect in inhibiting the proliferation of 4T1 mouse breast cancer cells. Cell apoptosis assays showed that FA-FC-PTX/DOX had the highest apoptosis rate in all drug formulations, indicating that it had high ability to induce cell apoptosis. Furthermore, it was found that FA-FC-PTX/DOX exhibited better inhibition effect on 4T1 tumor growth compared with free PTX/DOX combination or non-folate drug-loaded micelles and there was no obvious side effect on the 4T1 tumor-bearing mice. Thus, this work provides a promising drug co-delivery system for targeted therapy of breast cancer.
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CARBON
·
2015
·
IF 6.196
Yunfeng Zhan,Jilin Huang,Zhipeng Lin,Xiang Yu,Dongrong Zeng,Xiaoxue Zhang,Fangyan Xie,Weihong Zhang,Jian Chen,Hui Meng
By doping graphene with iodine and ammonia, I and N co-doped graphene (ING) is prepared and used as metal free catalyst for the oxygen reduction reaction (ORR) both in alkaline and acidic media. The as-prepared catalyst exhibits striking activity in alkaline with an onset potential of 0.945 V (vs. RHE) which is the same with commercial Pt/C catalyst. In acidic media the ING shows a competitive activity. In both alkaline and acidic media the ORR is four-electron process catalyzed by ING. The superior ORR performance of the ING can be attributed to the synergetic doping effect of I and N. Based on its outstanding performance the ING can work as a metal free catalyst for fuel cell with much lower cost than currently used Pt/C catalyst.
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危险品化学品经营许可证（带存储）