JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Ziyu Zhang,Yanlin Li,Rong Fang,Hongfang Zhang
Gold nanorods with different aspect ratios were synthesized by pH regulation. • A DNA three-way junction was employed for target recognition and AuNRs introduction. • A highly sensitive amperometric biosensor was developed for MUC-1 detection. Mucin 1 (MUC-1) is a valuable tumor-associated transmembrane glycoprotein. Electrochemical biosensor is a convenient tool for the quantitative detection of MUC-1 in biological samples. In this research, a new MUC-1 biosensor is developed based on gold nanorods conjugated with DNA three-way junction. The sandwiched biosensor is fabricated by covalent anchoring the anti-MUC-1 antibody on an electrode surface which is modified with the nanocomposite of silver nanoparticles and chitosan. In the presence of MUC-1, gold nanorods can be specifically introduced onto the sensor surface because of one aptamer tail of the DNA three-way junction, which is conjugated with gold nanorods via two thiol terminals. Then, the reductive current of H 2 O 2 monitored by amperometry increases drastically with the increase of MUC-1 concentration. Under the optimized conditions, the biosensor exhibited a linear response over the range of 100 fg·mL −1 to 1 μg·mL −1 , with a low detection limit of 54.3 fg·mL −1 . The biosensor showed good reproducibility, with an RSD of 4.7% for the response of five independently fabricated sensors. The detection recovery in spiked serum samples ranges from 87.1% to 110.0%, demonstrating the application potential of the proposed strategy for ultrasensitive electrochemical detection of MUC-1 in biological samples. Download: Download high-res image (193KB) Download: Download full-size image
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BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Haoyue Lv,Xiaoman Duan,Zhaoyu Han,Haozhen Yu,Biwu Liu
Fluorescent DNA assays are promising in disease diagnosis, environmental monitoring, and drug screening, encompassing both heterogeneous and homogeneous assay types. Nevertheless, heterogeneous assays suffer from tedious washing steps and slow reaction kinetics , whereas homogenous assays require well-designed fluorophore pairs to modulate signal off/on. Herein, we developed a cost-effective and efficient quencher-free fluorescent DNA assay using an aqueous two-phase system (ATPS). Using a strand-displacement reaction, we showed that similar sensing performance could be achieved at a much lower cost. Furthermore, the unique crowding environment in ATPS accelerated strand-displacement reactions by up to six-fold and reduced DNA amplification time from 120 min to 30 min. Our assay demonstrated robust sensing in serum environments and successful detection of miRNA extracted from cells. This innovative assay format has the potential for biosensor development with both heterogeneous readout and rapid reaction kinetics in various applications.
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IMMUNITY
·
2023
·
IF 32.4
Yanfei Hou,Zhimeng Wang,Peiyuan Liu,Xubiao Wei,Zhengyin Zhang,Shilong Fan,Lulu Zhang,Fangping Han,Yikang Song,Ling Chu,Conggang Zhang
Lipid metabolism has been associated with the cyclic guanosine monophosphate (GMP)-AMP synthase (cGAS) stimulator of interferon genes (STING) DNA-sensing pathway, but our understanding of how these signals are integrated into a cohesive immunometabolic program is lacking. Here, we have identified liver X receptor (LXR) agonists as potent inhibitors of STING signaling. We show that stimulation of lipid metabolism by LXR agonists specifically suppressed cyclic GMP-AMP (cGAMP)-STING signaling. Moreover, we developed cyclic dinucleotide-conjugated beads to biochemically isolate host effectors for cGAMP inhibition, and we found that LXR ligands stimulated the expression of sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A), which is a 2′3′-cGAMP-degrading enzyme. Results of crystal structures suggest that cGAMP analog induces dimerization of SMPDL3A, and the dimerization is critical for cGAMP degradation. Additionally, we have provided evidence that SMPDL3A cleaves cGAMP to restrict STING signaling in cell culture and mouse models. Our results reveal SMPDL3A as a cGAMP-specific nuclease and demonstrate a mechanism for how LXR-associated lipid metabolism modulates STING-mediated innate immunity.
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ADVANCED FUNCTIONAL MATERIALS
·
2023
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IF 19
Yangcenzi Xie,Mingyang Chen,Xinyu Liu,Xiaoming Su,Ming Li
Epigenetic DNA methylations are early and frequently observed events in a diversity of diseases such as cancer. Despite the considerable clinical values for cancer liquid biopsy, quantitative analysis of DNA methylations remains a major challenge due to the lack of rapid, sensitive detection techniques. Here, an artificial intelligence-assisted label-free surface-enhanced Raman spectroscopy (SERS) (iMeSERS) biosensor is reported for simultaneous quantification of C 5 -methylcytosine ( 5m C) level and methylation ratio in DNA samples. This method utilizes the plasmonic Pickering emulsions as the biosensing platform for label-free SERS detection, formed upon the addition of a sub-microliter DNA sample to the hydrophobic Au nanostar-containing n -decane. Distinct spectral signatures of monophosphates of canonical deoxyribonucleotides (dNMPs) and the common methylation modification 5-methyl-2′-deoxycytidine (d 5m CMP) are identified and distinguished by the iMeSERS biosensor. The deep learning algorithms trained with SERS signatures of dNMPs and d 5m CMP are then applied to the quantitative analysis of global DNA methylation. The exceptional capability of the deep learning-driven approach is demonstrated for simultaneous quantification of the methylation ratio and level using a sub-microliter volume of DNA samples. This work shows the power of label-free SERS techniques combined with deep learning algorithms for quantitative analysis of epigenetic DNA modifications with great promises for clinical diagnosis.
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BIOMEDICINE & PHARMACOTHERAPY
·
2023
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IF 7.5
Xin-Yu Fan,Guang Shi,Yun-Peng Zhao,Jing-Jing Yang,Juan Feng
Glutamate plays a crucial role in cognitive impairments after ischemic stroke. There is a scarcity of information about how glutamate-induced activation of cAMP-response element binding (CREB) signaling pathway regulates both the negative and positive regulators of synaptic plasticity. Recent studies have demonstrated the involvement of prominent epigenetic repressors, such as MeCP2 and DNMTs, in stroke. Neuroprotective effects of oxytocin against ischemia have been previously reported, while the underlying mechanism is still elusive. In this research, the possible role of CREB-mediated DNA hypermethylation and the potential mechanism of oxytocin in a rat model of permanent middle cerebral artery occlusion (pMCAO) were assessed. Adult male Sprague-Dawley rats were pretreated with intraperitoneal injection of oxytocin at the onset of pMCAO. The effects of oxytocin on spines and the expression levels of synaptic genes were determined. The regulatory effects of oxytocin on glutamate level, N-methyl- D -aspartate receptors (NMDARs), its downstream CREB pathway, and global or gene-specific DNA methylation status were evaluated by immunofluorescence, co-immunoprecipitation, and chromatin immunoprecipitation, respectively. We found that CREB could act as a common transcription factor for MeCP2 and DNMT3B after ischemic stroke. Oxytocin dose-dependently deactivated NR2B-related CaM-CREB pathway and inhibited DNA hypermethylation at the CpG islands of Ngf gene in pMCAO-operated rats. Moreover, oxytocin prevented pMCAO-induced reduction in the number of spines and neural cells. DNA hypermethylation in Ngf gene contributed to the cognitive deficits post-stroke. The neuroprotective effects of oxytocin against ischemia could be attributed to inhibiting glutamate release, providing additional evidence on the mechanism of oxytocin against ischemic stroke.
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International Journal of Nanomedicine
·
2023
·
IF 8
Weimin Fang,Jinghao Wang,Xiaocong Ma,Ni Shao,Kunlin Ye,Dong Zhang,Changzheng Shi,Liangping Luo
Background Olaparib, a poly (adenosine diphosphate-ribose) polymerase (PARP) inhibitor has demonstrated promising efficacy in patients with triple-negative breast cancer (TNBC) carrying breast cancer gene (BRCA) mutations. However, its impact on BRCA wild-type (BRCAwt) TNBC is limited. Hence, it is crucial to sensitize BRCAwt TNBC cells to olaparib for effective clinical practice. Novobiocin, a DNA polymerase theta (POLθ) inhibitor, exhibits sensitivity towards BRCA-mutated cancer cells that have acquired resistance to PARP inhibitors. Although both of these DNA repair inhibitors demonstrate therapeutic efficacy in BRCA-mutated cancers, their nanomedicine formulations’ antitumor effects on wild-type cancer remain unclear. Furthermore, ensuring effective drug accumulation and release at the cancer site is essential for the clinical application of olaparib.Materials and Methods Herein, we designed a progressively disassembled nanosystem of DNA repair inhibitors as a novel strategy to enhance the effectiveness of olaparib in BRCAwt TNBC. The nanosystem enabled synergistic delivery of two DNA repair inhibitors olaparib and novobiocin, within an ultrathin silica framework interconnected by disulfide bonds.Results The designed nanosystem demonstrated remarkable capabilities, including long-term molecular storage and specific drug release triggered by the tumor microenvironment. Furthermore, the nanosystem exhibited potent inhibitory effects on cell viability, enhanced accumulation of DNA damage, and promotion of apoptosis in BRCAwt TNBC cells. Additionally, the nanosystem effectively accumulated within BRCAwt TNBC, leading to significant growth inhibition and displaying vascular regulatory abilities as assessed by magnetic resonance imaging (MRI).Conclusion Our results provided the inaugural evidence showcasing the potential of a progressively disassembled nanosystem of DNA repair inhibitors, as a promising strategy for the treatment of BRCA wild-type triple-negative breast cancer.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
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IF 10.588
Xiaofang Li,Susu Zhang,Ying Wu,Luying Jiang,Wenxi Zhang,Xiaoqiang Qiao,Hongyuan Yan,Hongjian Zhou,Baokun Tang
DNA toxic compounds (DNA-T-Cs), even in trace amounts, seriously threaten human health and must be completely eliminated. However, the currently used separation media face great challenges in removing trace DNA-T-Cs. Based on the functional advantages of deep eutectic solvents (DESs) and the natural features of biomass (BioM), a series of Poly(DES)@BioMs functioning as adsorbents were prepared for the removal of aromatic/hetero-atomic DNA-T-Cs at the ppm level. After optimisation of experimental conditions, the removal efficiency for DNA-T-Cs ranged from 92.4% to 96.0% with an initial concentration of 20.0 ppm, a temperature of 30 °C, duration of 30 min, and pH of 7.0. The removal processes between the DNA-T-Cs and Poly(DES)@BioMs are well described in the Temkin equilibrium and second-order kinetic adsorption models, and the desorption processes are well shown in the Korsmeryer–Peppas equilibrium and zero-order kinetic models. Molecular simulations revealed that the removal interactions include hydrogen bonding, π–π stacking, and hydrophobic/hydrophilic effects. The removal efficiency for the DNA-T-Cs at 8.0 ppm in industrial sewage ranged from 69.7% to 102%, while the removal efficiency for the DNA-T-Cs standing alone at 20.0 ppm in a methyl violet drug solution was 95.4%, confirming that the Poly(DES)@BioMs effectively removed trace DNA-T-Cs in field samples.
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ACS Omega
·
2021
·
IF 3.512
Ruijie Wang,Yuan Qin,Xin Liu,Yangzi Li,Zhenfeng Lin,Rong Nie,Yifei Shi,Huayu Huang
A selective electrochemical biosensor was developed for detecting carbendazim (CBZ) based on well-dispersed colloidal boron nitride (BN) nanocrystals and gold nanoparticles (Au NPs). BN was synthesized by “solvent cutting” to modify a glassy carbon electrode (GCE), and Au NPs were then electrodeposited. A single-stranded oligonucleotide with methylene blue (MB) was modified to the electrode surface through gold–sulfur bonds. A double-stranded DNA was formed in the presence of an aptamer. The aptamer chain can specifically bind to the target CBZ. When the aptamer binds to CBZ, the electroactive substance MB labeled at one end of the complementary chain can effectively contact the electrode surface. Detection of CBZ is realized by simultaneously monitoring the MB signal enhancement. The CBZ concentration was determined in a wide linearity range from 0.1 ng mL–1 to 100 μg mL–1, with a low detection limit of 0.019 ng mL–1. This biosensor exhibited excellent selectivity and acceptable repeatability and was applied in cucumber, kiwifruit, and water samples with good recoveries, demonstrating that the strategy has remarkable potential and offers a good platform for CBZ detection.
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ANALYTICAL LETTERS
·
2021
·
IF 2.329
Minting Wang,Liting Zhao,Yanmei Wen,Yulian Wu,Yubin Li
Simian virus 40 (SV40) causes multiple diseases due to its low immunogenicity and strong infectivity. Here, the development of a label-free fluorescent sensor designed to detect double-stranded DNA (dsDNA) via a functionalized molecular beacon (MB) produced using triplex DNA and guanine (G)-quadruplexes is described. A SV40-specific dsDNA sequence was used as the target dsDNA (T-dsDNA). The MB was designed to comprise a G-quadruplex sequence, a complementary homopyrimidine sequence for T-dsDNA, and a partially complementary sequence for G-quadruplex sequence. Addition of T-dsDNA forces the MB to unfold, forming triplex DNA, and releasing the G-quadruplex sequence that binds to N-methyl mesoporphyrin IX (NMM), producing a signal. The G-quadruplex/NMM complex augments the signal at 610 nm and allows qualitative SV40 detection. Under optimal conditions, this strategy demonstrates a low limit of detection (0.4 nM). Compared to traditional biosensors, this sensor is enzyme-free, rapid, selective, sensitive, and was used to determine T-dsDNA in serum, suggesting that this strategy has applications in clinical analysis and for other viruses.
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RSC Advances
·
2021
·Vol. 11
·期 33
·20046-20056
·
IF 3.361
Yuqi Huang,Shuhui Zhao,Wenxiu Zhang,Qiuyue Duan,Qi Yan,Hu Fu,Liang Zhong,Gang Yi
Nucleic acids are genetic materials in the human body that play important roles in storing, copying, and transmitting genetic information. Abnormal nucleic acid sequences, base mutations, and genetic changes often lead to cancer and other diseases. Meanwhile, methylated DNA is one of the main epigenetic modifications, which is considered to be an excellent biomarker in the early detection, prognosis, and treatment of cancers. Therefore, a multifunctional electrochemical biosensor was constructed with sturdy tetrahedral tripods, which assisted multiple tandem hairpins through base complementary pairing and effective ultra-sensitive detection of targets (DNA, microRNA, and methylated DNA). In the experiments, experimental conditions were optimized, and different DNA concentrations in serum were detected to verify the sensitivity of the biosensor and the feasibility of this protocol. In addition, microRNA and DNA methylation were detected through different designs of tetrahedral tripods (TTs) that capture probes to prove the superiority of this scheme. A sturdy pyramid structure of TTs extremely enhanced the capture efficiency of targets. The targets triggered the one-step isothermal multi-tandem amplification reaction by incubating multiple hairpin assemblies. To our knowledge, a combination of two parts, which greatly reduced background interference and decreased non-specific substance interference, has appeared for the first time in this paper. Moreover, the load area of electrochemical substances was significantly increased than that in previous studies. This greatly increased the detection range and detection limit of targets. The electrochemical signal responses were generated in freely diffusing hexaammineruthenium(III) chloride (RuHex). RuHex could adhere to the DNA phosphate backbone by a powerful electrostatic attraction, causing increased current responses.
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ANALYTICA CHIMICA ACTA
·
2021
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IF 6.558
Zhongdong Wu,Zihan Li,Gangyong Li,Xuchao Zheng,Yuhe Su,Yan Yang,Youwei Liao,Zongqian Hu
Enzymes are promising electrocatalysts in many biological processes . We proposed two strategies, co-immobilization and three-dimensional (3D) space design, to strengthen electron transfer (ET). In this research, DNA base and CNT were mixed in an aqueous solution; then the mixture was dried and ground. Finally, the powder was annealed in N 2 to obtain DNA derived N-doped 3D conductive network ( [email protected] ). [email protected] immobilized mediators on itself through adsorption. Such 3D space structure shows high activity toward a set of critical electrochemical reactions and high-performance in enzymatic biofuel cells (EBFCs). It is found that [email protected] conductive network possesses an interconnected porous structure and well-developed porosity. As a result, the membrane-less EBFCs equipped with enzyme/mediator co-immobilization [email protected] bioelectrodes were measured in a model 5 mM glucose-containing aqueous solution, human serum, and rabbit whole blood, respectively, which can generate 0.34, 0.078, and 0.15 mW cm −2 power density, respectively. The constant-current discharge method carried out in a model 5 mM glucose-containing aqueous solution shows that the discharge time reached 19 h at a discharge current density of 0.01 mA cm −2 . The membrane EBFCs can deliver a high open circuit voltage of 0.68 V, a short-circuit current density of 2 mA cm −2 , and a maximum power density of 0.5 mW cm −2 .
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高影响因子
ANALYST
·
2021
·Vol. 146
·期 17
·5413-5420
·
IF 4.616
Long Jiang,Xiaonan Qu,Wenyu Sun,Manru Zhang,Yeru Wang,Yu Wang,Yijin Zhao,Fenfen Zhang,Yuning Leng,Su Liu,Jinghua Yu,Jiadong Huang
In this study, a novel, rapid and ultrasensitive fluorescence strategy using the three-dimensional (3D) dynamic DNA walker (DW)-induced branched hybridization chain reaction (bHCR) has been proposed for the detection of ampicillin (AMP). The sensing system was composed of an Nt·Bbvcl-powered DNA walker blocked by an AMP aptamer, hairpin-shaped DNA track probe (TP) and four kinds of metastable hairpin probes as the substrates of bHCR, which triggered the formation of the split G-quadruplex as the signal molecule. Due to the reasonable design, the specific binding between AMP and its aptamer activated the DW, and the DW moved on the surface of the gold nanoparticles (AuNPs) with the help of Nt·Bbvcl to produce primer probes (PPs), which induced bHCR. The products of the bHCR gathered two split G-quadruplex sequences together to form one complete G-quadruplex. The formed G-quadruplex emitted a strong fluorescence signal in the presence of thioflavin-T (ThT) to achieve the purpose of detecting AMP. The sensitivity of this method was greatly improved by the use of the 3D DNA walker and bHCR. The split G-quadruplex enhanced the signal-to-noise ratio (SNR). Under the optimal experimental conditions, a good correlation was obtained between the fluorescence intensity of the sensing system and the concentration of AMP ranging from 5 pM to 500 nM with a limit of detection (LOD) of 3.68 pM. Simultaneously, the method has been applied to the detection of antibiotics in spiked milk samples with satisfactory results.
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