Analytical Methods
·
2025
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IF 2.6
Changqing Du,Anran Li,Jinye Qiu,Jiarui Sun,Xiaokun Wang,Wei Xu,Maofeng Zhang,Dapeng Wang
Quantitative mRNA expression levels are essential for differential and clinical diagnoses. In this work, we present an apurinic/apyrimidinic endonuclease 1 (APE1)-assisted SERS sensing platform for rapid and ultrasensitive detection of TK1 mRNA. This platform utilizes a hairpin probe that contains a TK1 mRNA capture sequence along with an apurinic/apyrimidinic (AP) sites, which is specifically recognized and cleaved by APE1. Upon introduction of the target mRNA, hybridization occurs between the mRNA and the AP probe (HAP) within the hairpin structure, forming a doublestranded complex. APE1 then cleaves the HAP at the specific AP sites within the double-stranded complex, leading to a measurable change in the SERS intensity of the FAM-labeled HAP. Based on this principle, the developed SERS sensing platform demonstrates a highly linear response for TK1 mRNA detection across a wider range from 1 fM to 10 nM, with a detection limit (LOD) of 0.2 fM. Moreover, the SERS sensing platform shows great potential for the precise and quantitative detection of TK1 mRNA in human serum, which could be instrumental for mRNA-related research and the early clinical diagnosis of diseases.
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Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 30
·4630-4637
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IF 5.047
Huijie Wang,Wenwen Ouyang,Xuerui Zhang,Jing Xue,Xiaoran Lou,Ranran Fan,Xiaonai Zhao,Lianqi Shan,Tingting Jiang
The challenge in antimicrobial photothermal therapy (PTT) is to develop strategies for decreasing the damage to cells and increasing the antibacterial efficiency. Herein, we report a novel theranostic strategy based on bacteria-induced gold nanoparticle (GNP) aggregation, in which GNPs in situ aggregated on the bacterial surface via specific targeting of vancomycin and bioorthogonal cycloaddition. Plasmonic coupling between adjacent GNPs exhibited a strong “hot spot” effect, enabling effective surface enhanced Raman scattering (SERS) imaging of bacterial pathogens. More importantly, in situ aggregation of GNPs showed strong NIR adsorption and high photothermal conversion, allowing enhanced photokilling activity against Gram-positive bacteria. In the absence of bacterial strains, GNPs were dispersed and showed a very low photothermal effect, minimizing the side effects towards surrounding healthy tissues. Given the above advantages, the bioorthogonal theranostic strategy developed in this study may find potential applications in treating bacterial infection and even multidrug-resistant bacteria.
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Catalysts
·
2020
·Vol. 10
·期 7
·746
·
IF 3.52
Yuxiang Lu,Jikai Mao,Zelin Wang,Yazhou Qin,Jianguang Zhou
Controllable morphological metal catalytic materials have always been a focus in research. In the previous work, hexapod AgCl was successfully synthesized. In this paper, hexapod Ag@AgCl microstructures with diverse Ag contents are prepared through NaBH4reduction. They are characterized by scanning electron microscopy (SEM) and the element distribution is proved by an energy dispersive X-ray spectrometer (EDS). They are porous dendritic microstructures with a large specific surface area and a rough surface, which display high catalytic performance and surface-enhanced Raman spectroscopy (SERS) activity. Furthermore, the hexapod Ag@AgCl microstructure is devoted as a dual catalyst to monitor the reduction of 4-nitrothiophenol (4-NTP) to 4-aminothiophenol (4-ATP) in situ using SERS. Ultraviolet–visible (UV–Vis) spectroscopy was employed to evaluate the catalytic performance of the hexapod Ag@AgCl microstructures. The consequences show that the velocity of reaction became faster with a corresponding increase in silver content and the reaction mechanism was cleared. In addition to preparing a good catalyst, this work also promotes the combination of micro-nano materials and spectroscopy technology.Keywords:Ag@AgCl microstructure;in situ monitoring;surface-enhanced Raman spectroscopy;4-nitrothiophenol reduction
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Jie Yang,Ling Zhou,Xin-Yue Wang,Gao Song,Li-Jun You,Ju-Mei Li
Herein, the robust core-satellite Ag/TiO 2 /Ag composite nanospheres were successfully developed. We firstly fabricated large silver nanoparticles with sufficient plasmonic activity as cores and then encapsulated uniform titanium dioxide interlayer shell around Ag cores, which serves as the highly functionalized substrates to achieve uniform and plentiful surface chemical properties. Next, the outer shell of small silver satellites was formed in-situ via controllable reduction of AgNO 3 by n -butylamine, which acts as the remarkable enhancer for surface enhanced Raman scattering (SERS). The Ag/TiO 2 /Ag composite nanospheres with exceptional SERS sensitivity were easily prepared via facilely tuning the coverage and grain sizes of silver satellites. Then the optimized Ag/TiO 2 /Ag composite nanospheres were employed to quantitatively detect methylene blue (MB) in solution. The results showed good linear relationship between SERS intensities and logarithm concentrations of MB and the limit of detection is low to 10 −10 M. Moreover, the highly uniform and robust Ag/TiO 2 /Ag composite nanospheres display outstanding reproducibility (RSD = 4.02%) and highly storage stability. Owing to the intrinsic catalytic activity of small Ag satellites, Ag/TiO 2 /Ag nanospheres were further applied to effectively follow the degradation reaction process of crystal violet by SERS. Altogether, the core-satellite Ag/TiO 2 /Ag nanospheres present multiple SERS application potential in various fields, such as quantitative chemical analysis, trace detection of pollutants and in-situ reaction monitoring.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Sasa Peng,Zhilong Zhang,Jialin Guo,Tianchen Ma,Dongli Liu
In this paper, we developed a simple, rapid and sensitive method for detection of thiram on apple surfaces by surface enhance Raman spectroscopy (SERS) combined with chemometric methods. Ag NCs (Ag nanocubes) were firstly prepared by a sulfide-mediated polyol method. Then the flexible and adhesive Ag NCs@PDMS substrates were obtained by combining Ag NCs self-assembled films with PDMS films. Thiram residues on apple surfaces were transferred to the substrate using adhesion properties of Ag NCs@PDMS. And the SERS spectra were obtained by Raman microscopy and analyzed with chemometric methods. The results were analyzed by principal component analysis (PCA), for the limit of detection (LOD) of thriam on apple surfaces was 0.01 ppm. Principal component regression (PCR) and partial least squares regression (PLSR) were explored to develop quantitative models. Both models represented higher correlation coefficients (close to 1), but PLSR models exhibited better predictive performance, with the correlation coefficient was 0.99282 with a low root mean squared error of calibration (RMSEC = 0.438) and root mean squared error of validation (RMSECV = 0.597). The developed SERS method based on Ag NCs@PDMS substrate provide a simpler and more sensitive way to monitor thiram on apple surfaces.
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TALANTA
·
2024
·
IF 5.6
Zhao-He Chen,Nan Sun,Jian-Ping Li,Jia-Wei Zheng,Ya-Hao Wang,Xiao-Shun Zhou,Bin Zheng
As the extensive use of antibiotics has led to the rapid spread of antibiotic resistance, there is an urgent need for quantitative assessment of antibiotic residues in the environment. Surface-enhanced Raman spectroscopy (SERS) has emerged as a rapid and cost-effective detection method, but it suffers from the high variability in signal intensities, its quantitative detection remains challenging. Herein, we have developed a SERS calibration substrate with a silent region internal standard, enabling simultaneous and reliable quantitative detection of three commonly antibiotics of penicillin potassium (PP), tetracycline hydrochloride (TCH) and levofloxacin (LEV). The calibration substrate is made by assembling Au @ 4-mercaptobenzonitrile (4-MBN) @ SiO 2 on silicon wafer. The chemically-inert silica shell allows the substrate to remain SERS active for more than 24 weeks in the air. The v C ≡ N of 4-MBN in the silent region of 1800–2800 cm −1 provides an effective reference for correcting Raman signal fluctuations. The relative SERS intensity by normalizing to the internal standard (IS) of v C ≡ N shows a linear response against to the logarithmic concentration with a correlation coefficient of 0.997, 0.976, 0.998 and a limit of detection (LOD) of 26.9, 28.2, 2.4 nM for PP, TCH and LEV, respectively. Furthermore, simultaneous detection and principal component analysis (PCA) of these antibiotics in lake water with a concentration range of 1–100 mg/L can achieve a sensitivity and specificity of 100 %. This novel quantitative technique of using this SERS calibration substrate shows promises as a high-throughput platform for multiple trace antibiotics analysis.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yu-ning Liu,Jian-jun Li,Guo-jun Weng,Jian Zhu,Jun-wu Zhao
Morphology regulation of heterodimer nanoparticles and the use of their asymmetric features for further practical applications are crucial because of the rich optical properties and various combinations of heterodimers. This work used silicon to asymmetrically wrap half of a gold sphere and grew gold branches on the bare gold surface to form heterogeneous nano pineapples (NPPs) which can effectively improve Surface-enhanced Raman scattering (SERS) properties through chemical enhancement and lightning-rod effect respectively. The asymmetric structures of NPPs enabled them to self-assemble into the monolayer membrane with consistent branch orientation. The prepared substrate had high homogeneity and better SERS ability than disorganized substrates, and achieved reliable detection of malachite green (MG) in clams with a detection limit of 7.8 × 10 −11 M. This work provided a guide to further revise the morphology of heterodimers and a new idea for the use of asymmetric dimers for practically photochemical and biomedical sensing.
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ACS Sensors
·
2024
·
IF 8.2
Jinru Xiao,Jiacheng Ding,Chenyang Sun,Dingbin Liu,Hongmei Gao,Yaqing Liu,Yang Lu,Xia Gao
Sports doping remains a significant challenge in competitive sports. Given that urine analysis is the standard for detecting doping, developing rapid, sensitive, accurate, and high-throughput methods for stimulant detection in urine is crucial. Surface-enhanced Raman scattering (SERS) tag-based immunoassays have emerged as powerful analytical tools known for their high sensitivity and specificity, holding particular promise for stimulant detection in urine samples. However, both the Raman signals of typical SERS tags and sample matrices are within the Raman fingerprint region (<1800 cm–1), which could lead to spectrum overlap, potentially reducing detection accuracy and sensitivity. By recognizing this, we designed a competitive immunoassay that integrates two types of zero-background SERS tags and magnetic separation. These innovative SERS tags exhibit distinctive Raman peaks within the Raman-silent region (1800–2800 cm–1), effectively mitigating potential spectrum overlap with background sample signals. Moreover, magnetic separation not only enhances operational simplicity but also improves the system’s anti-interference capability. Using clenbuterol (CL) and higenamine (HM) as model targets, the SERS-based competitive immunoassay demonstrated sensitive detection of individual CL or HM standards, with limits of detection (LODs) of 0.87 and 0.71 pg/mL, respectively. In multiplex mode, CL and HM can be simultaneously detected with LODs of 1.0 and 0.81 pg/mL, respectively. Furthermore, the recovery rates in urine samples ranged from 83 to 116% (relative standard deviation, RSD ≤ 6.4%) for CL and from 82 to 103% (RSD ≤ 5.1%) for HM, further confirming the reliability of the SERS-based immunoassay for practical applications.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Zheng Feng,Peng Zhang,Xue Yang,Xiaowei Guo,Jiamin Yu,Liangguang Du,Jimin Shao,Hui Liang,Hong Jiang
Surface-enhanced Raman spectroscopy (SERS) has great potential for rapid detection of trace levels of pesticides in food. Scientists have much interested in this analytical technique due to its sensitivity, rapid detection, simplicity, and low cost. However, most of researchers are only focusing on the detection of a single pesticide residue . In this paper, we firstly report the simultaneous detection of two pesticide residues in tobacco leaves by using composite SERS substrate of SiO 2 @Ag nanoparticles . The entire analysis time is less than 10 min. The detection results show satisfactory linearity in the range of 0.1–25 mg/kg and 1–25 mg/kg with the limit of detection of 0.1 mg/kg and 1 mg/kg for carbendazim and flumetralin in tobacco samples, respectively. The recovery rates of carbendazim and flumetralin in the tobacco leaves are 85.2 ∼ 112.12 % and 90.38 ∼ 113.42 %, respectively. The detection results also show good uniformity and reproducibility, with a relative standard deviation(RSD) value of 4.4 % between substrates. Our reported substrate combined with portable Raman spectrometer provides a powerful tool for simultaneous detection of multiple pesticide residues for food safety.
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Materials Today Communications
·
2024
·
IF 3.7
Guoliang Qi,Lei Zhao,Jinxin Liu,Cheng Tian,Shusheng Zhang
Micro/nano plastics (MNPs) are widely recognized as a global environmental threat. Due to the lack of viable and reliable analytical methods, the identification and visualization of microplastics , especially nanoplastics , remains difficult to achieve, especially for single particle detection. Here, this study offered a simple, high-sensitive, and low-cost technique for MNPs identification using surface-enhanced Raman scattering (SERS) based on two-dimensional AuNPs thin films . This work realized the single particle detection of polystyrene (PS) and polymethyl methacrylate (PMMA), owing to the hot spot region created by the electromagnetic amplification of two-dimensional AuNPs thin films. The SERS-active substrate had a high SERS property in the single particle detection of MNPs as small as 200 nm. Moreover, this method can be used for the SERS mapping as a rapid detection technique for MNPs. In additon, this SERS-active substrate had good stability and reusable performance after 5 cycles. These findings indicated that this SERS strategy based on two-dimensional AuNPs thin films was an efficient and rapid method to identify nanoplastics in environment. And we believed that this kind of recyclable SERS sensor may create a universal strategy for the detection of MNPs at trace level.
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ACS Sensors
·
2024
·
IF 8.2
Ying Xu,Zhiyu Li,Yuanrong Liao,Jun Wang,Tong Zhang,Xifu Liu,Yang Zhang
Noble metal nanoparticle (NMP)-based composite substrates have garnered significant attention as a highly promising technique for surface-enhanced Raman scattering (SERS) in diverse scientific disciplines because their remarkable ability to amplify and functionalize Raman signals has positioned them as valuable tools for molecular detection. However, optimizing the size and distribution of NMPs has not received sufficient emphasis because of challenges associated with the precise control of deposition and the modulation of reducing rates during growth. In this research, we achieved the optimized size and spatial patterns of AgNWs on electrospun poly(vinylidene fluoride) (PVDF) nanofibers by utilizing a polydopamine (PDA) layer as a mild and controllable reduction mediator, by which the size and density of the AgNWs could be relatively precisely manipulated, achieving a dense distribution of effective “hot spots”. On the other hand, harnessing the inherent piezoelectric properties of the electrospun PVDF nanofibers further boosted the LSPR effect during the SERS test, forming a flexible dual-enhancing composite SERS substrate with excellent sensitivity. In addition to addressing structural aspects, exploiting synergistic systems capable of transferring external energy or forces to enhance the SERS performances presents a compelling avenue to broaden the practical applications of SERS. The dual-enhanced substrate achieved an exceptional enhancement factor (EF) of 1.05 × 108 and a low detection limit (LOD) of 10–10 M during the SERS test. This study focuses on integrating NMPs with electrospun piezoelectric polymer nanofibers to develop a dual-enhancing SERS substrate with excellent sensitivity and practicality. The findings provide valuable insights into controllably depositing NMPs on electrospun polymer fibers and hold significant implications for the development of highly sensitive and practical SERS substrates across various applications.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Houyang Ge,Qizhen Chen,Sheng Chen,Cong Li,Xingen Gao,Wanqiang Fu,Hongyi Zhang,Fuqiang Wang,Xianqiong Gong,Junzheng Wu,Juqiang Lin
Plasma-free amino acid (PFAA) profiling is crucial for monitoring and evaluating cancer progression. However, there remains a lack of sensitive, simple, and direct methods for rapid PFAA detection. In this study, we developed a vapor-deposited 3D flexible porous structure named FPS Ag - Coated serving as a high-performance surface-enhanced Raman spectroscopy (SERS) platform for ultrasensitive detection of micro PFAAs (10 −4 M). The surface of the platform contains abundant hydroxyl functional groups, which not only confer exceptional flexibility but also significantly minimize background signal interference. The 3D FPS architecture provides densely packed hotspots for Raman signal enhancement while demonstrating excellent cost-effectiveness and scalability for mass production. We systematically evaluated the platform's detection capability using four representative amino acids: fluorescent amino acids (L-tyrosine and L-tryptophan), weakly fluorescent amino acid (L-phenylalanine), and non-fluorescent amino acid (L-serine). Across a concentration range of 10 −1 to 10 −4 M, the platform effectively measured characteristic SERS signals with linear correlation, enabling real-time monitoring and quantitative analysis of PFAAs. When applied to clinical plasma samples from cancer patients, the platform successfully identified PFAAs-specific SERS signal. Subsequent principal component analysis (PCA) achieved discrimination between different cancer types and healthy volunteers with an accuracy exceeding 83.3 %. This work presents a high-performance SERS platform for PFAA recognition, offering a highly adaptable flexible material that could facilitate the clinical translation of SERS-based diagnostics.
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