LUMINESCENCE
·
2022
·Vol. 37
·期 8
·1290-1299
·
IF 2.613
Dan Li,Peipei Zhou,Yufei Hu,Gongke Li,Ling Xia
In recent years, fluorescent organic–inorganic hybrid nanomaterials have received much interest as potential fluorescent sensor materials. In this study, fluorescent organic–inorganic hybrid nanomaterials (POSS@ANT) were created using polyhedral oligomeric silsesquioxane as the precursor and 9,10-bromoanthracene as the monomer. The morphology and composition of POSS@ANT, as well as its pore characteristics and fluorescence properties were studied. POSS@ANT displayed steady fluorescence emission at an excitation wavelength of 374 nm. Next, a β-carotene fluorescence sensor was developed using the capacity of β-carotene to quench the fluorescence of POSS@ANT. The quenching process is linked to acceptor electron transfer and energy transfer, and the sensor has a high selectivity for β-carotene. This β-carotene fluorescence analysis method that we established has a linear range of 0.2–4.3 mg/L and a detection limit of 0.081 mg/L. Finally, it was used to quantify β-carotene in health products, the recovery rate was 91.1–109.9%, the relative standard deviation (RSD) was 2.2–4.3%, and the results were comparable with the results of high-performance liquid chromatography. The approach is reliable and can be used to determine β-carotene in health products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Linlin Zhang,Jie Wang,Chenglong Zhao,Feijin Zhou,Cheng Yao,Chan Song
The nanozymes-based sensing platforms have been attracted in recent years due to their outstanding performances in the practical applications. In this work, the magnetic Fe nanoparticles (NPs) with peroxidase-like activity was developed for the fast detection of glutathione (GSH). The Fe NPs were facilely prepared through the reduction of iron trichlorrde hexahydrate (FeCl 3 ‧6H 2 O) by sodium borohydride (NaBH 4 ) at room temperature , exhibiting a merit of simplicity. Significantly, the achieved Fe NPs have high peroxidase-like catalytic activity . At the room temperature, the colorimetric substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) can be rapidly oxidized by Fe NPs with the help of H 2 O 2 . After incubated for just 30 s, the catalytic system could achieve a colored solution. Thus, the Fe NPs-based sensing systems can be employed in the development of the colorimetric biosensors with the naked-eye detection.
✓ 已验证
产品关联
DOI
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 6
·868-875
·
IF 2.613
Feifei Li,Pengfei Fan,Xinbei Chen,Xi Lin,Can Liu,Congcong Hu,Shengyuan Yang,Fubing Xiao
Sensitive detection of doxorubicin (DOX) is critical for clinical theranostics. A novel ratiometric fluorescence strategy based on the inner filter effect (IFE) has been established for the sensitive detection of DOX by designing a ratiometric fluorescence probe. In the presence of DOX, the fluorescence intensity of copper nanoclusters (CuNCs) at 485 nm decreases, and the fluorescence intensity of carbon dots at 560 nm increases. Therefore, DOX can be quantitatively detected by measuring the ratio of the fluorescence intensities at 560 and 485 nm ( F 560 / F 485 ). The F 560 / F 485 ratio exhibits a linear correlation with the DOX concentration in the range from 1.0 × 10 −8 M to 1.0 × 10 −4 M with the detection limit of 3.7 nM. Furthermore, this method was also successfully applied to the analysis of DOX in human plasma samples, affording an effective platform for drug safety management.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sustainable Chemistry and Pharmacy
·
2022
·
IF 5.464
Yao Chen,Fangxiang Xu,Mingmei Pang,Xiangzi Jin,Huihao Lv,Zuguang Li,Mawrong Lee
In recent years, deep eutectic solvents (DESs) as green and sustainable solvents have been widely used in the effective extraction of natural products. Usually, DESs are synthesized by heating and stirring method which takes a long time and energy. In this case, a microwave assisted preparation technique was used to form DESs for saving time and energy. And the DESs as pretreatment solvents were combined with microwave-assisted hydrodistillation (MAHD) for the extraction of essential oils (EOs) from the clove buds in this work. The maximum essential oils yield of 4.60% was obtained with the microwave power of 600 W, the pretreatment time of 5 min and the hydrodistillation time of 40 min using a deep eutectic solvent composed of choline chloride and lactic acid (molar ratio with 1:2). In addition, the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS), a total of 40 compounds were identified. Compared with MAHD, water-based MAHD and traditional hydrodistillation (HD), the content and composition of essential oil extracted by DES-based MAHD were higher. Thus, the combination of deep eutectic solvents with a microwave-assisted technique in this study provided an eco-friendly way of extracting essential oils.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Hongyu Chen,Huimeng Zheng,Wen Li,Qingfeng Li,Bin Hu,Nan Pang,Fengshou Tian,Lin Jin
Upconversion nanoparticles (UCNPs) have shown great promise in bioanalytical applications owing to their excellent optical properties . Generally, most analytical applications are based on the fluorescence resonance energy transfer (FRET) principle to quench the fluorescence of UCNPs. However, each UCNP contains thousands of emission center ions, and most of them exceed the FRET critical distance, which hinders FRET efficiency and leads to a low signal-to-background ratio (SBR). Herein, a novel nanoprobe for the detection of Xanthine (XA) based on inner filter effects (IFE) and cascade signal amplification strategy was constructed by decorating UCNP with trypsin-chymotrypsin-stabilized gold nanoparticles-gold nanoclusters (Try-chy-AuNPs-AuNCs) monometallic nanohybrids . The Try-chy-AuNPs-AuNCs prepared by ultrafast (3 min) and green synthesis method have efficient upconversion fluorescence quenching ability (the quenching efficiency up to 90.9%), which can effectively improve the SBR of the probe, so as to improve the sensitivity. In addition, the Try-chy-AuNPs-AuNCs have a unique spatial structure, which can effectively prevent the interaction between large-size biothiol (glutathione) and the probe, thus improving its selectivity. Besides, combined with the excellent optical performance of UCNPs and cascaded signal amplification strategy, the sensitivity of the probe can be further improved. Under the optimized conditions, the linear response range of the probe was obtained from 0.05 to 50 μM, 0.06–80 μM and with the low detection limit of 22.6 nM and 26.3 nM for H 2 O 2 and XA, respectively. Meanwhile, the developed method has been further applied to the detection of XA in human serum with satisfactory results.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Li Ji,Leheng Zhang,Huiyi Yang,Shan Liang,Junkang Pan,Yikui Zou,Shijia Li,Qinglan Li,Suqing Zhao
In view of the current public health hazards of food-borne pathogens, it is urgent to develop a rapid detection method with high sensitivity, good specificity and operational convenience, as well as to determine an effective sterilization strategy. Herein, versatile gold-ruthenium nanocomposites modified with antibody ( [email protected] Ncs) have been constructed for the sensitive detection of Salmonella typhimurium (S. typhimurium) via the lateral flow immunochromatographic assay (LFIA). [email protected] Ncs based LFIA exhibited a wide detection range from 2.9 × 10 6 CFU/mL to 2.9 × 10 11 CFU/mL with the limit of detection of 9.8 × 10 4 CFU/mL for S. typhimurium, and displayed excellent specificity. In addition, [email protected] Ncs irradiated with 808 nm (500 mW/cm 2 ) near-infrared light (NIR) had a significant antibacterial effect within only 5 min, attributed to its high photothermal conversion efficiency of 54.14%. Therefore, both sensitive detection of S. typhimurium and effective NIR-triggered photothermal sterilization were achieved by using versatile [email protected] Ncs, showing great prospects in the field of pathogen detection and treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Tan Jingying,Geng Weifu,Li Junde,Wang Zhen,Zhu Shaohao,Wang Xiuzhong
The mimic enzyme has become a research hotspot in recent years because of its advantages of high stability, convenient preparation, and low price. In this article, Co3O4 nanosheets synthesized by a simple hydrothermal method possess the characteristics of a peroxidase-like activity. The results demonstrated that 3,3′,5,5′-Tetramethylbenzidine (TMB) could be oxidized by H2O2 to produce a typical blue product (oxTMB) which has a strong absorption at 650 nm wavelength with the help of the Co3O4 nanosheets. Thus, a simple and sensitive colorimetric detection method for H2O2 was established with a good linear relationship (2–200 μM) and a low limit of detection (0.4 μM). Meanwhile, the colorimetric product can effectively quench the fluorescence emitted by Ru(bpy)32+. Therefore, a colorimetric and fluorescence dual detection mode photochemical sensor for H2O2 detection is constructed based on the principle of the inner filter effect (IFE) between the colorimetric product (oxTMB) and Ru(bpy)32+. It can effectively avoid the false positive problem of a single detection mode. In the presence of glucose oxidase, glucose can be catalyzed to produce gluconic acid and H2O2; therefore, the sensor can also be used for the determination of glucose with a good linear relationship (0.02–2 μM) and a low limit of detection (5 nM). Experimental results showed that the sensor has a high sensitivity and strong anti-interference ability which can be used for the detection of actual samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xiao Li,Hui Dong,Qiaolin Fan,Ke Chen,Dongke Sun,Tao Hu,Zhonghua Ni
Hybrid transition metal-based compounds show excellent performance in the field of enzyme-free glucose sensing. Herein, a one-step, green, facile, highly efficient microwave-assisted strategy for fabricating bimetallic Co/Zn metal–organic frameworks (denoted as ZIF67/ZIF8) is proposed. The structural characterization and phase investigation of ZIF67/ZIF8 are assessed by microscopically studies, elemental mapping, X-ray diffraction and X-ray photoelectron spectroscopy. The proportion of Co and Zn elements of ZIF67/ZIF8 has been elaborately regulated, resulting in enhanced electro-oxidation performance toward glucose compared to mono-metal organic frameworks. The synergy effect between Co and Zn proves to be crucial for optimizing electroanalytical ability, which endows outstanding glucose sensing performance, including high sensitivity of 833.61 μA/mM/cm 2 , wide linear range up to 5 mM and low detection limit to be 6.5 μM. Furthermore, to demonstrate the practical application capability of ZIF67/ZIF8, real-time analysis in human serum sample is conducted, which confirms its good anti-interference ability and recovery rate. With the advantages of low cost, ease of production and high environment tolerance, this work provides a versatile method to obtain non-noble metal based biosensing materials for non-enzymatic glucose detection.
✓ 已验证
产品关联
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2022
·
IF 8.129
Xiaowen Ge,Hong Li,Mengyuan Liu,Zhenyu Zhao,Xin Jin,Xiaolei Fan,Xin Gao
Ethoxymethylfurfural (EMF) is a promising fuel additive and biofuel which can be produced via catalytic direct alcoholysis of fructose. In this work, microwave-responsive catalysts were prepared by carbonization of cellulose (via either hydrothermal treatment or calcination) then functionalized with SO 3 H group (via impregnation using concentrated sulfuric acid), which exhibit remarkable performances in one-pot catalytic conversion of fructose to EMF with ethanol. After a 8 h reaction under microwave irradiation at 75 °C, about 80.3% fructose conversion was achieved with the EMF yield of 61.2% for the carbon catalyst prepared by the hydrothermal treatment, which is much higher than that achieved by the catalysis promoted by conventional heating. Further investigation showed that with a microwave-responding catalyst, microwave irradiation encourages the formation of local hot-spots on the catalyst surface, thus facilitating the collision of reactant molecules with catalysts to improve the catalytic efficiency. This work demonstrates the potential of microwave-responsive heterogeneous catalysts for process intensification of valorization of bio-derived chemicals under microwave irradiation.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Hao Chao,Zixin Zhou,Wenting He,Meng Li,Xiaoyu Yuan,Ping Su,Jiayi Song,Yi Yang
Although capsule-like materials as host carriers for enzyme encapsulation have been a hot topic in recent years, creating an ideal microenvironment for enhanced enzymatic performance is still a formidable challenge. Herein, we created a template-free method to in situ encapsulate natural enzymes in hollow covalent organic framework (COF) capsules at room temperature. The COF crystallites migrated from the inner core and self-assembled at the outside walls during the inside-out Ostwald ripening process, retaining the enzymes in the cavity. The adjustable hollow structure of the enzyme@COF capsule allowed the basic vibration of the enzyme to maintain a certain degree of freedom, thus significantly enhancing the enzymatic bioactivity. The hollow enzyme@COF capsule has large mesoporous tunnels allowing the efficient transport. In addition, the enzyme encapsulated in the capsule showed superior activity and ultrahigh stability under various extreme conditions that may lead to enzyme inactivation, such as high temperature, organic solvents, chelates, and the denaturing agent. Finally, the prepared hollow GOx@COF capsule was used for electrochemical sensing of glucose in human serum, and the electrochemical sensor exhibited high selectivity and satisfactory test results. This research not only provides a new way for COFs to encapsulate enzymes but also has potential applications in biocatalysis and biosensing, making artificial organelles possible.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Meng Li,Chenxi Liu,Liang Qi,Hongsheng Liu
Hollow-structured nanomaterials, featuring a porous crystalline structure and hollow interior, are conducive to achieving a compartmentalized architecture comprising diverse functional entities. In this study, we have constructed a novel fluorescent enzyme nanocomposite derived from the nanosized hollow ZIF-8 (H-ZIF-8) colloidosomes via a diffusion strategy. Insights into the underlying mechanisms revealed that the diffusion process was driven by mesopore properties and electrostatic interactions. It has also been demonstrated that the molecular sieving property of the colloidosome shell induced an exceptional compartmentalized structure, where nitrogen-doped graphene quantum dots (N-GQDs) were anchored onto the outer surface of the nanosized H-ZIF-8 colloidosomes, whereas glucose oxidase (GOx) was homogeneously encapsulated in the entire cavity. The resultant nanocomposite inherited the mesopore properties of the colloidosome exoskeleton, catalytic ability of GOx, and fluorescence properties of N-GQDs. In particular, the porous properties of the nanosized H-ZIF-8 colloidosomes favored enrichment to target analytes, whereas the compartmentalized GOx and N-GQDs served as a “promoter” (initiate the biochemical reaction) and “signal unit” (intuitive observation) in the glucose probing system, respectively. Furthermore, anchoring N-GQDs onto the outer surface of the nanosized H-ZIF-8 resulted in tailoring of the fluorescence property of the nanocomposite. In the part of glucose sensing, the proposed fluorescence nanosensor could well quantify glucose within a range of 10 to 100 μM with a lower limit of detection of 5.4 μM. This proposed strategy presents opportunities to engineer novel metal–organic framework-based multifunctional nanocomposites and create new applications by combining their unique functions.
✓ 已验证
产品关联
DOI
高影响因子
ACS Food Science & Technology
·
2022
·
IF 0
Wenxia Zhang,Baojie Zhu,Holly Childs,Monica Whent,Liangli Yu,Pamela R. Pehrsson,Jing Zhao,Xianli Wu,Shaoping Li
Sweet corn is an important source of carbohydrates in the human diet. In this study, the effects of the two most commonly used cooking methods (boiling and steaming) on the contents of individual sugars, sugar alcohols, oligosaccharides, total starch, resistant starch (RS), phytoglycogen, water-soluble carbohydrates (WSC), and dietary fibers (DF) were investigated using yellow and bicolor sweet corn samples. Four simple sugars, sucrose, glucose, fructose, and maltose, were identified. Boiling and steaming did not alter the concentrations of sucrose but resulted in the decrease of other three minor sugars. Sorbitol and mannitol were identified as the primary sugar alcohols. Sorbitol decreased in cooked samples, while mannitol remained unchanged regardless of the cooking method. Oligosaccharides and phytoglycogen were not detected in either variety. Boiling and steaming did not alter the content of WSC, total starch, or higher-molecular-weight DF but led to a significant reduction of RS and total DF. This study provided understanding on how cooking may affect the health benefits and quality of sweet corn.
✓ 已验证
产品关联
DOI
危险品化学品经营许可证（带存储）