BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Jing-Wen Zhou,Xiao-Bing Zheng,Heng-Su Liu,Bao-Ying Wen,Yi-Chuan Kou,Lin Zhang,Jing-Jin Song,Yue-Jiao Zhang,Jian-Feng Li
Abnormal levels of uric acid (UA) in urine serve as warning signs for gout and metabolic cardiovascular diseases, necessitating the monitoring of UA levels for early prevention. However, the current analytical methods employed suffer from limitations in terms of inadequate suitability for home-based applications and the requirement of non-invasive procedures. In this approach, creatinine, a metabolite with a constant excretion rate, was incorporated as an endogenous internal standard (e-IS) for calibration, presenting a rapid, pretreatment-free, and accurate strategy for quantitative determination of UA concentrations. By utilizing urine creatinine as an internal reference value to calibrate the signal fluctuation of surface-enhanced Raman spectroscopy (SERS) of UA, the quantitative accuracy can be significantly improved without the need for an external internal standard. Due to the influence of the medium, UA, which carries a negative charge, is selectively adsorbed by Au@Ag nanoparticles functionalized with hexadecyltrimethylammonium chloride (CTAC) in this system. Furthermore, a highly convenient detection method was developed, which eliminates the need for pre-processing and minimizes matrix interference by simple dilution. The method was applied to the urine detection of different volunteers, and the results were highly consistent with those obtained using the UA colorimetric kit (UACK). The detection time of SERS was only 30 s, which is 50 times faster than UACK. This quantitative strategy of using urinary creatinine as an internal standard to correct the SERS intensity of uric acid is also expected to be extended to the quantitative detection needs of other biomarkers in urine.
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高影响因子
NANO LETTERS
·
2025
·
IF 9.1
Shuai Zhang,Shuo Meng,Renjie Tan,Ke Zhang,Zhuang Wang,Xiaoyun Xu,Chuanwei Zhi,Xinshuo Liang,Jinlian Hu
Real-time monitoring and evaluation of muscle atrophy are vital for efficient treatment and effective diagnosis. Although some systems have been developed to monitor loss of muscle mass or strength, they are bulky and nonspecific, cannot be applied to the body, and cannot assess the degree of muscle atrophy. Our research focuses on developing a wireless multisensor wearable system (WMWS) for on-body monitoring and assessment of atrophy, which utilizes a single-electrode triboelectric nanogenerator (S-TENG) and electrochemical creatinine (CREA) sensor to achieve real-time acquisition of plantar pressure and interstitial fluid (ISF) CREA concentration. In addition, LSTM (long short-term memory) and SVM (support vector machine) machine learning (ML) algorithms also prove that our multisensor strategy can effectively improve the recognition rate of different degrees of atrophy (the highest accuracy reached 92.32%). Overall, our work makes it possible to monitor and grade muscle atrophy remotely in real time.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jie Fu,Jiawang Wang,Yihong Ding,Shouzhe Deng,Cherie S. Tan,Shuang Li
Pheochromocytoma is a neuroendocrine tumor characterized by excessive catecholamine secretion, leading to hypertension and complications. Vanillylmandelic acid (VMA), a stable metabolic product of catecholamines (CA), is primarily excreted in urine, with levels rising early in the disease or prior to tumor recurrence. Therefore, rapid and accurate detection of urinary VMA is essential for the early diagnosis and monitoring of pheochromocytoma. Herein, we developed a wearable physicochemical-sensing system for non-invasive in situ monitoring of biomarkers associated with pheochromocytoma. The system integrates cuffless blood pressure (BP) monitoring technology and electrochemical biosensor, utilizing screen-printing to create high-precision multi-channel electrodes. It can be integrated with diaper to detect blood pressure and biomarkers including VMA, creatinine, and pH, aiming to improve urine analysis efficiency and enhance patient experience. The VMA-to-creatinine concentration ratio was employed as an evaluative metric, with measurements calibrated against external variables including pH, temperature, and ionic strength, enabling random urine sample collection and analysis. The resulting sensor demonstrates sensitive and linear responses within the concentration ranges of 10 nM–100 μM for VMA, 100 nM–10 mM for creatinine, and 4–9 for pH. The cuffless blood pressure monitoring patch simultaneously collects dual-lead ECG and PPG, extracts features, and inputs them into a prediction model to estimate BP values. The correlation between the estimated blood pressure value of the model and the actual blood pressure value is 0.9207 (systolic blood pressure, SBP) and 0.9015 (diastolic blood pressure, DBP), respectively. We conducted further tests on the electrochemical biosensor's selectivity, reproducibility, and stability, evaluating the potential of this strategy in effectively monitoring pheochromocytoma.
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ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shanshan Zhang,Hongfeng Wang,Yuke Zheng,Yelan Yao,Tianyu Li,Yukun Ma,Yue Zhou,Zhiyang Chen,Yiming Wei,Lu Fang,Xiangmei Chen,Xuesong Ye,Jianhui Zhou,Bo Liang
The global epidemic of diabetes has popularized at-home glucose monitoring. However, detecting biomarkers for serious diabetes complications, such as diabetic nephropathy (DN), still necessitates frequent hospital visits, invasive blood analyses, and bulky laboratory equipment. Herein, a wireless wearable device based on an integrated paper-based patch (IPP) for non-invasive monitoring of three key DN biomarkers: glucose, creatinine, and uric acid, via in situ sweat analysis, is demonstrated. The disposable IPP realizes the all-in-one preparation of a high-performance electrochemical sensor array, an efficient microfluidic module, and iontophoresis electrodes, enabling reliable multi-parametric on-body sweat monitoring at rest. Notably, all electrodes on the IPP are treated with acrylamide-doped PEDOT: PSS conductive ink, imparting high conductivity to the IPP while preserving the porous structure of the paper substrate, thereby maximizing the electrochemically active area for highly sensitive detection of biomarkers. The usability of the wearable device is validated in assessing metabolic status in healthy participants after dietary intake and during physical exercise, as well as dialysis efficacy during hemodialysis in patients with end-stage renal disease. The wearable device for detecting DN biomarkers in sweat may facilitate at-home disease monitoring and the real-time assessment of hemodialysis efficacy for DN patients.
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2025
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IF 2.7
Qiaolin Wang,Yulong Shi,Xiaojian Li,Junyao Zhang,Zefeng Hua,Xinyan Yang,Zhongfa Sun,Zhengbo Qin,Xianfeng Zheng
An integrated miniature time-of-flight mass spectrometer (TOF-MS) system coupled with a pocket-size 3D-printed laser-induced acoustic desorption (LIAD) source is described. This 3D-printed LIAD source utilizes only a miniature deceleration motor to achieve two-dimensional motion of the target surface, simplifying the source structure and improving the long-term stability of mass spectrometry measurements. It has been successfully applied to analyze the model molecule creatinine and ingredients in an energy beverage (Red Bull), where main natural nutrients were clearly identified. By adjusting the 3D printing parameters, the source can be readily adapted to various application scenarios requiring different sizes. This work provides a simple and efficient 3D-printed source injection strategy for subsequent investigations in molecular reaction dynamics.
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Chemosensors
·
2025
·Vol. 13
·期 9
·340
·
IF 3.7
Hongmei Gao,Yourong Zhao,Yuhong Xie,Yiying Wang,Jie Che,Daojiang Gao,Zhanglei Ning
In this study, a novel carbon quantum dots-functionalized UiO-66 composite was successfully prepared via the post-synthetic modification method and further developed into a ratiometric fluorescent probe for detecting uric acid. The composite demonstrates excellent structural and luminescent stability under challenging environmental conditions. As a ratiometric fluorescent probe, its recognition principle relies on the ratio of response signals from two different fluorescent emission centers in the composite. In the presence of uric acid, the fluorescence emission intensity at 430 nm from CDs did not change significantly. However, the fluorescence intensity at 545 nm from Tb3+ions decreased remarkably. This material was evaluated for its capacity to sense urinary components and was shown to specifically recognize uric acid over a wide concentration range (0~5 × 10−3M). Moreover, it exhibited strong resistance to interference and high sensitivity in uric acid detection. The detection limit (LOD) was determined to be 0.102 μM through quantitative analysis. The sensing mechanism was validated through spectral overlap and fluorescence lifetime analysis, which can be attributed to the fluorescence resonance energy transfer (FRET) process. This ratiometric fluorescent probe provides an efficient and reliable strategy for detecting the biomarker uric acid.
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