Science China-Chemistry
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2025
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IF 9.7
Chen Qianqian,Xu Haoxing,Zhao Bingyue,Patehebieke Yeersen,Zhao Zerun,Wang Xiao
The selective nucleoside synthesis through direct condensation between nucleobase and unactivated sugar in aqueous media has long been a formidable obstacle for the early chemical transformations hypothesized to construct the RNA World, due to product complexity and the low selectivity of the desired isomer. The preference of β-furanoside, the extant RNA component, has never been documented. Here we report an aqueous ribosylation mediated by divalent transition-metal ions, which unprecedentedly enhances the selectivity of furanosyl ribonucleosides as opposed to the traditionally predominating pyranosyl ones, with the furanoside/pyranoside ratio reaching up to 9/1. Furthermore, β-furanoside stands out for the first time as the most prevalent isomer in the presence of zinc and ammonium salts. The mechanism of this selectivity is elucidated with density functional theory calculations. The selectivity for β-furanoside can be further elevated via the adsorption on a zinc mineral. These findings exhibit as the first example that the selection of nucleosides with the desired, canonical configuration might be completed in the early stage of the RNA-forming process as opposed to later evolution, promoted by simple and widespread transition-metal minerals. The study may also find its value in synthetic chemistry as a guideline for developing a sustainable and selective glycosylation process.
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Probiotics and Antimicrobial Proteins
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2025
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IF 4.4
Huang Fang,Lu Yun-hui,Xiong Jie,Zeng Xin-an,Huang Yan-yan,Wang Lang-hong
The escalating global burden of hyperuricemia, driven by modern dietary patterns and sedentary lifestyles, has prompted urgent exploration of novel therapeutic strategies, with particular emphasis on developing targeted probiotic interventions as a potential alternative to conventional pharmacological approaches. This study successfully isolated Limosilactobacillus fermentum FOSU YHD19 from fermented foods, demonstrating remarkable in vitro uric acid reduction of 12.90% along with high-level degradation of nucleosides. A comprehensive safety evaluation confirmed the absence of harmful enzymatic activities (including nitroreductase, decarboxylase and tryptophanase), hemolytic properties, or antibiotic resistance. The strain exhibited strong stress tolerance, surviving in acidic conditions (pH 2.0), with exposure to gastric juice, and when in the presence of 0.1% bile salts. Additionally, YHD19 demonstrated potent antibacterial activity against multiple pathogenic strains, including E coli , S. aureus , L. monocytogenes , and S. enteritidis . Genomic analysis revealed three nucleoside hydrolases and six transporter systems that collectively facilitate the hypouricemic activity. By ruling out the presence of key uric acid-synthesizing enzymes (xanthine dehydrogenase/oxidase and guanine deaminase), we can attribute the hypouricemic effect primarily to a nucleoside-scavenging mechanism. In addition, the genome harbors 91 stress-responsive genes implicated in acid/base adaptation, thermal shock response, and oxidative defense mechanisms, underscoring the strain’s resilience under gastrointestinal stresses. These findings position YHD19 as a safe and effective probiotic candidate for functional foods aimed at managing hyperuricemia, supporting its safety profile for potential probiotic applications.
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Journal of Analysis and Testing
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2025
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IF 7
Chen Jiajing,Lin Juan,Ouyang Dan,Lin Zian
Metabolomics, based on laser desorption/ionization mass spectrometry (LDI-MS), has been successfully utilized for the high-throughput metabolic analysis of biofluids. This technique shows great promises as a powerful tool for disease screening. Although various nanomaterials have been developed as alternatives to traditional organic matrices in LDI-MS, the signals of most metabolites are limited due to their low abundance in complex biofluids. Herein, four hydroxyl-functionalized covalent organic frameworks (COFs) were designed as substrates for LDI-MS, named COF-OH- n ( n = 0–3, demonstrating the number of hydroxyl units). Since hydroxyl groups can facilitate proton transfer and promote the ionization process effectively, hydroxyl-rich COF-OH-2 enabled sensitive and efficient LDI-MS analysis of serum directly, resulting in highly reproducible serum metabolic profiles (SMPs). An orthogonal partial least squares discriminant analysis (OPLS-DA) model was successfully used to distinguish 30 gout patients from 38 healthy volunteers, with an area under the curve (AUC) of 0.974. Furthermore, the roles of 12 biomarker candidates, which were characterised following statistical analysis, in the metabolic pathway of gout were initially explored. Furthermore, the roles of 12 biomarker candidates, which were characterized following statistical analysis, in the metabolic pathway of gout were initially explored. This work demonstrates the potential of COF-OH-2-assisted LDI-MS in clinical metabolic analysis and offers a rational design strategy for advanced LDI-MS substrates.
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ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Wen Li,Kaiming Ge,Ji-Na Hao,Yongsheng Li
Accurate identification of tumor metabolic markers is of great significance for the early screening of cancer. Luminescent lanthanide-based metal–organic frameworks (Ln-MOFs) are a fascinating assay platform for biomarkers, but the influence of their pore parameters on detection performance remains largely unexplored due to the lack of Ln-MOFs with an adjustable micro- and mesoporous structure. Herein, two isoreticular dual-emissive Ln-MOF-based sensors with microporous and hierarchically micromesoporous structures, namely, Eu-MMOF-BTC and Eu-HMMOF-BTC, respectively, are first constructed to systematically investigate their detection properties for adenosine (ADO, an important tumor marker in the diagnosis and prognosis of cancer). Remarkably, compared with the Eu-MMOF-BTC with micropores only, the Eu-HMMOF-BTC featuring both intrinsic micropores and large ordered mesopores affords greatly enhanced photoresponse efficiency and sensitivity toward ADO under both signal channels, benefiting from the facilitated access to active sites and improved preconcentration. Moreover, such an optimized dual-channel Eu-HMMOF-BTC sensor realizes real-time (15 s) and specific recognition of ADO in a complex urine environment without the interference of coexisting species, inherently improving the analysis reliability and accuracy. This study not only provides a facile strategy to regulate the sensing performance of MOF-based probes by ordered-pore-structure engineering but also provides inspiration for the design of high-accuracy probes for the noninvasive early screening of cancers.
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Yongxing Hu,Pengcheng Zhang,Ashraf Ali,Haokun Tang,Lijun He,Renyong Zhao,Wenfen Zhang,Shusheng Zhang,Wenjie Zhao
Background Hydrophilic interaction liquid chromatography (HILIC) offers an alternative approach for efficient separation of polar molecules on polar stationary phases. The development of hydrophilic stationary phases with novel zwitterionic structures represents a promising approach to improving the efficiency of separations and accommodating the analysis of polar compounds in complex samples. Results This work details the preparation and thorough evaluation of the novel zwitterionic stationary phase, Sil-DBO-PS, composed of a 1,4-diazabicyclo[2.2.2]octane cation and a sulfonate anion. The chromatographic behavior of Sil-DBO-PS was investigated under varying eluent conditions, including water phase ratio, salt concentration, and pH, while interaction mechanisms of Sil-DBO-PS were explored through Van't Hoff plots, Tanaka HILIC tests, and modified linear solvation energy relationships. These studies revealed that the zwitterionic group introduced a range of interactions, including hydrophilic partitioning, hydrogen bonding, and electrostatic interactions. The Sil-DBO-PS column outperformed two commercial columns in terms of separation efficiency for polar analytes, such as nucleosides, vitamins, and benzoic acids, highlighting its enhanced capability for polar compound separation. Furthermore, Sil-DBO-PS stationary phase demonstrated satisfactory reproducibility in the separation of hydrophilic compounds. Significance and novelty The development of the Sil-DBO-PS zwitterionic stationary phase significantly enhances the separation efficiency and selectivity for polar compounds in HILIC, offering superior performance over commercial columns for challenging analytes. This novel stationary phase, with its diverse interactions, provides a promising tool for the analysis of hydrophilic and complex samples.
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TALANTA
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2015
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IF 3.545
Xiao-Dong Cheng,Yan-Hong Hao,Xi-Tian Peng,Bi-Feng Yuan,Zhi-Guo Shi,Yu-Qi Feng
The present study described the preparation and application of zwitterionic stationary phases (ACS) with controllable ratio of positively charged tertiary amine groups and negatively charged carboxyl groups. Various parameters, including water content, pH values and ionic strength of the mobile phase , were investigated to study the chromatographic characteristics of ACS columns. The prepared ACS columns demonstrated a mix-mode retention mechanism composed of surface adsorption, partitioning and electrostatic interactions . The elemental analysis of different batches of the ACS phases demonstrated good reproducibility of the preparation strategy. Additionally, various categories of compounds, including nucleosides , water-soluble vitamins, benzoic acid derivatives and basic compounds were successively employed to evaluate the separation selectivity of the prepared ACS stationary phases. These ACS phases exhibited entirely different selectivity and retention behavior from each other for various polar analytes, demonstrating the excellent application potential in the analysis of polar compounds in HILIC .
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Food Bioscience
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2025
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IF 5.9
Zixi Li,Meng Dong,Libo Qi,Shan Shang,Yan Guo,Lei Qin,Baoshang Fu
Frying is the necessary step of fish soup production, which directly affects the flavor and nutritional quality of the soup. To investigate the effect of edible oil on the final quality of fish bone soup, five kinds of oil were employed in this study. The results showed that olive oil could promote the dissolution of glutamic acid and aspartic acid into the soup, the umami value elevated nearly four times. Meanwhile, olive oil and sunflower oil brought more volatile flavor substances, which contributed to the highly accepted flavor. Triglyceride O-16:0/18:1/18:2 (palmitic/oleic/linoleic triglycerides) was regarded as a potential precursor for the formation of flavor substances, such as 1-octen-3-one and 2-heptanone, which contribute to the specific flavor of fish bone soup. This study revealed that frying would improve the quality of fish bone soup by changing the color and flavor profile, guiding the selection oil for the industrial processing of fish bone soup.
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GEOCHIMICA ET COSMOCHIMICA ACTA
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2025
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IF 5
Yinyin Qian,Beibei Shi,Guangyao Li,Huaming Yang
Kaolinite nanoclay has long been recognized as playing a key role in the origin of life. However, the interaction of kaolinite (Kaol) with biomolecules in prebiotic chemistry and its catalytic mechanism still remain a great challenge. Herein, we comprehensively investigate the nucleoside interactions and phosphorylation processes on Kaol surfaces by a combination of wet-dry cycle experiments and first-principles calculations. The experimental results show that Kaol is able to promote the phosphorylation reactions of four typical nucleosides, but there are differences in the catalytic ability for different nucleosides. In this study, we considered two physicochemically different Kaol end-surfaces—the aluminium-hydroxyl surface (Kaol(001)) and the silica-oxygen surface (Kaol(00 1 ¯ ))—and find that Kaol(001) forms strong hydrogen bonds with the nucleoside, stabilizing the nucleoside that significantly reduces the kinetic barrier to phosphorylation. Moreover, ab initio molecular dynamics (AIMD) simulations showed that the Kaol(001)-nucleotide system is kinetically stable, highlighting its potential in protecting the reaction sites of prebiotic molecules and providing strong evidence for the role of Kaol in prebiotic chemistry.
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JOURNAL OF CHROMATOGRAPHY A
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2020
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IF 4.049
Yujie Zhang,Renling Lu,Ming Chen,Shouyong Zhou,Dabing Zhang,Haifeng Han,Mingliang Zhang,Hongdeng Qiu
An alternative method for efficient synthesis of urea-functionalized silanes was proposed on the basis of an N, N’ -carbonyldiimidazole-mediated acyl-transfer reaction between various amino-containing building blocks. The employment of different parent aminosilanes and alkylamines afforded an array of urea-containing silanes, which were subsequently immobilized onto silica gel to form corresponding urea-embedded alkyl stationary phases for high-performance liquid chromatography. The different substituents on the silicon core of the derivatized silane were found to significantly influence the final chromatographic behaviors. The comparative chromatographic characterization of thus-prepared silica packings with conventional octadecyl (C18) stationary phases revealed that the urea group was beneficial to suppress silanol activity towards basic probes, as well as to increase the water-compatibility of the alkyl stationary phases. The combination of a polar urea moiety and a non-polar long alkyl chain was favorable for an enhanced steric selectivity towards shape-constrained isomers. The polarizability-sensitive feature of such stationary phases made them good candidates for efficient separation of nitro-containing polar substances.
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