JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Binbin Zhang,Jiao Ma,Kaide Ou,Xin Cong,Hao Zou,Yonggui Liao,Yajiang Yang,Hong Wang
By loading 3-nitrophenylboronic acid (3-NPBA) which can react with saccharide, the fluorescence lanthanide metallogel can emit fluorescence signals at 398, 490 and 546 nm. Thus, a three-dimensional responsive fluorescence sensor array (H 6 L/Tb 3+ /3-NPBA) was prepared to recognize saccharides. Due to the typical pH responsiveness of the fluorescence behavior of the sensor array, it is suitable for H + generation systems, such as the reaction of 3-NPBA with cis -diol of saccharides. The response of fluorescence signal intensity to 15 different saccharides was measured, and the qualitative discrimination of those saccharides was investigated by principal component analysis (PCA). The results showed that PCA elliptic clusters did not overlap, even though the concentration of the saccharides was as low as 3 mmol/L, and the discrimination rate of each saccharide reached 100 %. The “double blind” experiment indicated that the sensor system has good ability to discriminate unknown component samples. Furthermore, the discrimination rate of fructose/glucose mixtures with varied mole ratios also reached 100 %. The method of Euclidean distance confirmed that there was a good linear correlation between PCA elliptic clusters of fructose and its concentration, which could realize the quantitative analysis of fructose. The results of PCA indicated that the elliptic clusters of tea drinks with various sugars and commercially available sugar-containing drinks were loosely distributed, which further confirmed that the sensor system can effectively discriminate the saccharides under the interference of complex components.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Jing Sun,Bo Zhu,Yixuan Sun,Zhihong Li,Ling Dong,Jianbo Chen
Scutellariae Radix (SR) is a common herb in Asia and Europe. In the clinical practice of traditional Chinese medicine, the raw SR is often stir-baked to partly scorch to reduce the side effects (stomach discomfort, diarrhea, etc.) but enhance some desired effects (such as the hemostatic activity). The scorching degree is crucial to assure the safety and efficacy of the scorched SR. Under-scorching is insufficient to adapt the adverse and favorable activities, while over-scorching can destroy all activities. Up to now, the scorching degree of SR is still determined by the manual observation of colors. Since the visual judgement is vulnerable to personal knowledge and experience, it is difficult to control the optimization and consistence of the scorching degree of SR. This research was designed to explore the potential indicators that can exactly reflect the scorching degree of SR and be measured objectively and quantitatively. A total of 15 morphological and chemical properties as well as Fourier transform infrared (FTIR) spectroscopy were included to profile the variations of the SR slices that were stir-baked at different temperatures for different times. According to the principal component analysis and statistical tests, 10 properties showed close relationships with the scorching degree of SR. As the increase of the heating time at the same heating temperature, the yield, L*, a*, b*, sucrose, baicalin and wogonoside decreased continuously and showed the monotone variation patterns. Meanwhile, 5-HMF, baicalein and wogonin showed the inflection variation patterns, which means these properties increased in the earlier stage but decreased in the later stage during the scorching process. FTIR spectra also revealed the variations of flavonoids and carbohydrates. The scorching degree of SR can be determined by the intuitive observation of FTIR spectral peaks at 1738 cm −1 , 1705 cm −1 , 1611 cm −1 , 1586 cm −1 , 1450 cm −1 , 1410 cm −1 and 1023 cm −1 . In summary, FTIR spectroscopy can be used for the rapid assessment of the scorching degree of SR, while the contents of flavonoid glycosides and aglycones may be used as the quality criteria of the scorched SR.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Liang Huang,Ziyu Shi,Daquan Li,Chenxing Jiang,Xuexue Wang,Mei Huang,Jing Wang,Bin Su
Considering that hue-recognition has drawn increasing research interests for visual detection because of its high accuracy and flexible analysis modalities, the exploration of a rational hue-recognition sensing system is highly demanded. Here, we report a hue-recognition-based lateral flow immunoassay (HLFIA) by ratiometric modulation of emission from two nanoprobes, in which red-emitting ruthenium(II)-doped dendritic silica colloids and green-emitting gold nanoclusters function as reporter and reference, respectively. The HLFIA strip can generate a visually discernible green-to-orange-red color gradient alternation, enabling the naked-eye semiquantitative inspection of successive C-reactive protein (CRP) concentration and concentration intervals (0–50, 50–150, and 150–500 ng mL–1) with a visual detection limit of 10 ng mL–1. By integrating with a customized smartphone-based portable imaging device and RGB color space recognition, the precise quantification of CRP can be achieved with a linear range of 0–100 ng mL–1 and a limit of detection of 0.64 ng mL–1. The HLFIA strip is robust and validated for CRP detection in real serum, holding great promise for noninstrumental and deprofessionalized point-of-care immunoassay of biomarkers.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Huihui Liang,Ying Luo,Yawen Xiao,Jianhui Xiong,Rongfang Chen,Yonghai Song,Li Wang
It is very urgent to construct an efficient neuron-specific enolase (NSE) immunosensor for the early diagnosis of small cell lung cancer (SCLC). Here, a sensitive NSE immunosandwich sensor was constructed based on dual signal amplification strategy via electrocatalytic oxygen reduction by iron-porphyrin covalent organic framework (COF p -Fepor NH2-BPA). The N -rich COF p -Fepor NH2-BPA containing abundant iron porphyrin ring was synthesized and connected to gold nanoparticles (AuNPs) via Au-N bond, and further successfully bound to Ab2 via Au-S bond to form beacon probe complex of COF p -Fepor NH2-BPA/AuNPs/Ab2. Another kind of COFDva-TAB with abundant vinyl was used to bond Ab1 for forming Ab1/COFDva-TAB/GCE by thiol-ene “click” reaction. The highlight of this NSE immunosensor is that COF p -Fepor NH2-BPA is a long-range ordered material enriched of iron porphyrin units owning obvious electrochemical signal. In the O2-saturated environment, the electrochemical signal is further amplified due to the electrocatalytic reduction of O2 by iron porphyrin units. Thus, the NSE immunosensor shows a wide detection range of 500 fg/mL–100 ng/mL, a low detection limit of 166.7 fg/mL and a high sensitivity of 8.82 j/lg(cNSE/ng mL −1 ). This work also provides effective dual signal amplification strategy for electrochemical immunoassay based on COFs.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Guangquan Mo,Qiuxia Li,Liu Zhu,Zhixiong Ruan,Songqing Mei
Herein, hollow and porous nano-dodecahedron embedded with cobalt oxide nanoparticles (HPND-CN) was prepared for the detection of rutin (Rut). HPND-CN was synthesized by the pyrolysis of zeolitic imidazolate frameworks (ZIFs). HPND-CN displayed a uniform rhombic dodecahedral morphology. For the main architecture, the original framework was carbonized after pyrolysis leading to a porous graphitized polyhedron with nitrogen doping. Notably, in the polyhedron, spherical cobalt oxide nanoparticles with more CoO and less Co 2 O 3 were evenly implanted. With embedded oxide nanoparticles, active doping species, polyhedral morphology, and porous graphitized structure, HPND-CN displayed a strong electrocatalysis towards the redox of Rut. A pair of well-defined symmetric peaks for Rut redox was clearly observed. Based on the electrocatalysis of HPND-CN, an efficient Rut sensor was developed with a low detection limit of 1.9 nM and broad detection range from 7.0 nM to 1.0 μM. HPND-CN prepared sensor also exhibited a superb selectivity showing no interference from common inorganic ions, carbohydrates, and amino acids. Besides, excellent repeatability, reproducibility and reliability were observed. Moreover, HPND-CN sensor was successfully applied to the measurement of Rut in health-care tablets with convinced results, indicating a substantial electrochemical sensing platform for the electrocatalysis and detection of macromolecules.
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Guan-Ting XU,Tian-Sheng ZHAO,Kun ZHANG,Li-Zhen GUO,Yong-Qin HE,Jian-Hua HU,Yi-Jing LIAO,Xi MAI,Na LI
Iron ions (Fe 3+ ) and ascorbic acid (AA) are of great significance to living organisms. Herein, carbon dots (C-dots)-based “off-on” fluorescent probe was designed for Fe 3+ and AA analysis. In presence of Fe 3+ , the fluorescence of C-dots was immediately quenched, and the quenching mechanism was deduced to be inner filter effect (IFE). The fluorescent C-dot probe shows response to Fe 3+ in range of 10–500 µM with a limit of detection (LOD) of 0.9 µM. Interestingly, when AA was introduced to the C-dots-Fe 3+ system, the fluorescence of C-dots was significantly recovered, which could be ascribed to the oxidation-reduction reaction between Fe 3+ and AA. Based “off-on” fluorescent C-dots probe, the linear range of 5–500 µM was obtained with LOD of 0.4 µM. Moreover, the AA-induced fluorescence enhancement can be recognized by smartphone, which greatly extends the application in resource-limited areas.
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Miao Zuo,Qiang Niu,Yanzhong Zhu,Sijin Li,Wenlong Jia,Zheng Zhou,Xianhai Zeng,Lu Lin
This study prepared a series of biochar-based catalysts using different parts of Larix principis-rupprechtii as feedstock , to promote the 5-Hydroxymethylfurfural (HMF) synthesis from glucose and fructose. Sulfonated biochar showed high converting efficiency of fructose in both dimethyl sulfoxide (DMSO) and aqueous-natural deep eutectic solvent (A-NADES) reaction systems, with HMF yield of 96.3% prepared from the pine needle-derived acidic catalyst under mild condition. Bifunctional acidic biochar catalysts containing both Lewis acid and Brønsted acid functional groups could effectively enhance the glucose conversion with 57.2% of the HMF yield obtained in A-NADES. After the detailed characterization of the biochar catalysts, the potential mechanism of the bifunctional catalyst for the synthesis of HMF by conversion of glucose and fructose was proposed. The possible promoting mechanism of carbohydrate conversion by A-NADES through intermolecular hydrogen bonding was also explored.
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1084
·
IF 5.743
Tao Tian,Wei-Yi Zhang,Hang-Yu Zhou,Li-Jing Peng,Xi Zhou,Hao Zhang,Feng-Qing Yang
In this study, the personal glucose meter (PGM) was first used as a fast and user-friendly meter for analyzing catechol (CA) based on the reduction of the mediator K3[Fe(CN)6] to K4[Fe(CN)6] in the glucose test strip. Then, an easy, low-cost, and convenient PGM-based method for detecting tyrosinase (TYR) activity and sodium benzoate (SBA) was developed on the basis of the TYR-catalyzed reaction. In this method, CA is oxidized to formo-benzoquinone by TYR, thereby reducing the residual amount of CA and the PGM readout. On the other hand, SBA can inhibit the oxidation of CA catalyzed by TYR and increase the residual amount of CA after the enzymatic reaction. Therefore, the activity of TYR is proportional to the difference in the PGM readout of CA, and the concentration of SBA is positively correlated with the residual amount of CA. After the relevant experimental conditions were systematically optimized, the proposed PGM-based method for the detection of TYR and SBA was successfully validated. The liner ranges are 1.0–103.3 U/mL and 6.25–1000 ppm, and the quantification limits are 1.0 U/mL and 6.25 ppm for TYR and SBA, respectively. Moreover, the spiked recovery tests in normal human serum and carbonate beverages (i.e., Cola, Sprite, and Fanta) were performed, and the recoveries (91.6–106.8%) further confirm the applicability of the PGM-based method in real sample analysis.Keywords:tyrosinase;sodium benzoate;personal glucose meter;enzyme sensor;carbonate beverages sample
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Yazhen Wu,Xiaoxiao Li,Zhengyu Jin,Birte Svensson,Yuxiang Bai
How to improve the solubility of linear dextrins (LD) and retain their characteristic helix amphiphilic cavities with flexible embedding capability, is a question worth exploring without adding new chemical groups. The strategy presented in this study is to attach a highly flexible (α-1 → 6) glucochain at the reducing end of LD by preparing a new type of dextrin, referred to as single-arm linear dextrin (SLD). In the actual synthesis, an (α-1 → 6) linked oligosaccharide of DP ¯ 10.7 (PDI = 1.28) was formed by extension of glucose units onto sucrose (2 M) by using L940W mutant of the glucansucrase GTF180-ΔN firstly. Next using γ-CD as glucosylation donor γ-CGTase extended this (α-1 → 6) glucochain with (α-1 → 4) bonds. SLD is a chimeric glucosaccharide comprising an (α-1 → 4) linked part ( DP ¯ 10.5) attached to the nonreducing end of an (α-1 → 6) glucochain as verified by enzyme fingerprinting and 1 H NMR. Furthermore, SLD was validated to show greatly improved solubility and dispersibility of resveratrol in water, as indicated by a 3.12-fold enhancement over the solubility in the presence of 0.014 M SLD. This study provided a new strategy for solving the solubility problem of LD and opens possibilities for new design of the fine structure of starch-like materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
·
2023
·
IF 6
Jirui Yang,Yanwei Zhang,Feng Shen,Xinhua Qi
Meerwein-Ponndorf-Verley (MPV) reaction has been widely acknowledged to be a mild catalytic transfer hydrogenation method for selective reduction of biomass-derived furfural to furfuryl alcohol . Herein zirconium-containing mesoporous composite catalyst was developed through the combination of sol-gel process and soft templating method, in which naturally abundant glucose and F127 triblock copolymer were used as the ligand and pore-forming agent, respectively. The hydroxyl groups in glucose molecules were demonstrated to be coordinated with zirconium ion during hydrolysis-condensation of zirconium precursor, leading to the even dispersion of zirconia nanoparticles in the composite catalyst. The composite catalyst was proven to be highly efficient for catalyzing MPV reduction of furfural with the maximum furfuryl alcohol yield of 93.4% at 180 °C within 180 min in 2-propanol, which served as both solvent and reductant . The composite also showed a good reusability . After five consecutive reaction cycles, furfuryl alcohol yield was slightly reduced from 93.4% to 77.7%, but it could be recovered to 92.8% after the reused composite was regenerated with dilute alkali solution. Batch experiments and systematic characterizations demonstrated that the excellent catalytic performance of composite catalyst was determined by multiple factors mainly including the stronger Lewis acidity, a considerable amount of basic site and the rich mesoporous structure. This study provides a facile strategy to prepare multifunctional composite catalysts using naturally abundant carbohydrates as the feedstocks for lignocellulosic biorefinery .
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Juwei Ao,Xuewei Pan,Qiang Wang,Hengwei Zhang,Kexin Ren,An Jiang,Xian Zhang,Zhiming Rao
α-Arbutin is extensively used in cosmetic industries. The lack of highly active enzymes and the cytotoxicity of hydroquinone limit the biosynthesis of α-arbutin. In this study, a whole-cell biocatalytic approach based on enzyme engineering and engineered cell modification was identified as effective in enhancing α-arbutin production. First, a sucrose phosphorylase (SPase) mutant with higher enzyme activity was obtained by experimental screening. Next, to avoid the oxidation of hydroquinone, we established an anaerobic process to improve the robustness of the cells by knocking out lytC, sdpC, and skfA in Bacillus subtilis and overcoming the inhibitory effect of a high concentration of hydroquinone. Finally, the engineered strain was used for biotransformation in a 5 L fermenter with batch feeding for 24 h. The final yield of α-arbutin achieved was 129.6 g/L, which may provide a basis for the large-scale industrial production of α-arbutin.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Chuanyi Cui,Jiang Li,Yi Wang,Peng Jiang,Weimin Li,Gaokuo Zhong,Xinyao Yang,Zheng Xing,Xin Wang,Ming Ma
Photoanode materials typically exhibit good stability and environmentally and economically properties in solar water splitting cells, while they always suffer from the narrow light absorption range, heavy surface charge combination and sluggish surface reaction kinetics . Herein, we design an efficient and reproducible photoanode by stacking amorphous carbon spheres (CSs) and cobalt-involved carbon nitrides (Co-CNs) on surface of TiO 2 nanorod arrays. CSs work as light sensitizer for broadening the light absorption range of photoanode and promoting the utilization of solar energy, which further improved by Co-CNs partially. In addition, CSs increase the conductivity of photoanode, as a transport channel for improving the charge migration. Co-CNs introduce many active sites on surface of photoanode for facilitated solar water oxidation . The best constructed TiO 2 /CSs/Co-CNs photoanode exhibits an impressive photocurrent density of ∼1.73 mA/cm 2 (at 1.23 V vs RHE). The staking method of various functional materials provides one promising strategy for the modification of catalysts.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）