TALANTA
·
2011
·
IF 3.722
Yang Wang,Jie Tang,Xiaoyu Luo,Xiaoya Hu,Chun Yang,Qin Xu
In this work, a kojic acid electrochemical sensor, based on a non-covalent molecularly imprinted polymer (MIP) modified electrode, had been fabricated in the lab-on-valve system. The sensitive layer was synthesized by cyclic voltammetry using o -phenylenediamine as the functional monomer and kojic acid as the template. The template molecules were then removed from the modified electrode surface by washing with NaOH solution. Differential pulse voltammetry method using ferricyanide as probe was applied as the analytical technique, after extraction of kojic acid on the electrode. Chemical and flow parameters associated with the extraction process were investigated. The response recorded with the imprinted sensor exhibited a response in a range of 0.01–0.2 μmol L −1 with a detection limit of 3 nmol L −1 . The interference studies showed that the MIP modified electrode had excellent selectivity. Furthermore, the proposed MIP electrode exhibited good sensitivity and low sample/reagent consumption, and the sensor could be applied to the determination kojic acid in cosmetics samples.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Zhiyong Zhao,Xianli Yang,Xiaoyan Zhao,Bing Bai,Chunxia Yao,Na Liu,Jianhua Wang,Changyan Zhou
The presence of Aspergillus and Penicillium species during the fermentation process of rice wine is inevitable. The toxic secondary metabolites produced by the two fungi need to be practically monitored for rice wine safety. In this study, we developed a fast and simple vortex-assisted dispersive liquid-liquid microextraction (VADLLME) for the extraction and preconcentration of major Aspergillus and Penicillium mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, mycophenolic acid, sterigmatocystin and verruculogen) in rice wine. The key parameters affecting the extraction efficiency of VADLLME procedure (pH value, type and volume of extraction and dispersive solvents) were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD) and desirability function. Target compounds were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Under the optimum conditions, good linearity ( R 2 >0.99) was achieved for all the analytes in the concentration range of 0.5–100 μg/L. The recoveries for spiked samples at three concentration levels were between 84.5% and 111.2%, with the relative standard deviations (RSD) lower than 20%. The limits of detection (LOD) and quantification (LOQ) ranged from 0.05 to 0.5 μg/L and from 0.1 to 1.0 μg/L, respectively. Simplicity in operation, low cost and low usage of organic solvents were the major advantages of the developed method. The method was successfully applied to the analysis of rice wine samples. It was found that mycophenolic acid had high incidence rate in rice wine, which needed to be continuously monitored.
研究领域:
食品安全
·
分析化学
·
微生物学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yuening Li,Huipeng Liang,Yuzheng Wu,Xinping Lin,Yiwei Dai,Chaofan Ji
Mixed-culture fermentation can enhance cider aroma, but direct co-culture often limits the persistence of non- Saccharomyces yeasts. Among these species, Metschnikowia koreensis is noteworthy for its strong protease and lipase activities that release aroma precursors, yet its interaction with Saccharomyces cerevisiae has not been well characterized. This study evaluated sequential inoculation of M. koreensis and S. cerevisiae with 0 h, 24 h, and 48 h delays. A 48-h delay (SM 48 ) extended M. koreensis viability to 9 days, increased ethanol production, and produced sensory attributes characterized by lower sourness and astringency and higher umami and richness. The SM 48 group produced the most abundant and diverse volatile compounds, with key fruity and floral esters elevated. OPLS-DA identified these esters as major discriminant contributors, whereas compounds commonly associated with off-flavors such as acetic acid and hexanal were reduced. Metabolic pathway analysis indicated enhanced Ehrlich and esterification pathways with reduced acetate production. Multi-method validation supported that SM 48 cider exhibits more complex volatile profiles, stronger fruity and floral notes, and improved color. These results demonstrate that controlled sequential inoculation is an effective approach for improving cider aroma complexity and overall product quality.
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 8
·1139-1144
·
IF 0
Gu Mengqiao,Liu Jiali,Li Delei,Wang Min,Chi Kuanneng,Zhang Xi,Deng Yan,Ma Yuchan,Hu Rong,Yang Yunhui
In this paper, a signal-off aptasensor was constructed based on the specificity of OTA and OTA aptamer (DNA2), it can detect the small molecule ochratoxin A (OTA). DNA1 chain with mercapto modified at 3′-end was firstly immobilized on gold electrode, which cross-fertilize with
DNA 2 to form double strands. 30 thymine T modified at the end of DNA2 were used as templates to synthesize Cu NCs which is peroxidase mimics and can catalyze hydrogen peroxide. Presence of OTA, it specifically binds to DNA2, which made DNA2 dissociated, and so did Cu NCs. The more OTA in
solution, the more the aptamers and Cu NCs dissociated from the gold electrode, the less copper nanoclusters remained on the electrode, and the smaller the current response value of the electrode measured by chronoamperometry. Thus, the quantitative determination of OTA can be achieved. This
aptasensor provides a simple and fast technique for OTA detection in red wine samples.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
生物传感器
✓ 已验证
DOI
Evidence-based Complementary and Alternative Medicine
·
2018
·
IF 2.064
Yinji Liang,Yupei Zhang,Yuanjun Deng,Shu Liang,Yifang He,Yanning Chen,Chan Liu,Chenli Lin,Li Han,Guifang Tu,Qinhe Yang
We evaluate the effects of the Chaihu-Shugan-San decoction on intestinal microbe dysbiosis and chronic metabolic inflammation via the NLRP3 pathway in NAFLD rats that were fed a high-fat diet. Twenty-four SD rats (male, six weeks old, 200 ± 20 g) were randomly divided into three groups: normal control group (NC group), high-fat diet-fed group (HFD group), and Chaihu-Shugan-San decoction intervention group (CH group). The NC group rats were given standard feed, the HFD group rats were all fed a high-fat diet (83% standard feed + 10% lard oil + 5% sucrose + 1.5% cholesterol + 0.5% cholate), and the CH group rats were given a HFD plus Chaihu-Shugan-San at 9.6 g•kg−1•d−1. Body composition, serum and liver lipids, inflammatory markers, intestinal microbial population, and the NLRP3 pathway-associated protein were assessed. The results showed that Chaihu-Shugan-San decoction significantly reduced body weight and total fat mass and the levels of serum LPS, TG, TNF-α, IL-1β, and IL-18, as well as liver TC, TG, TNF-α, IL-1β, and IL-18 (P < 0.05). The abundance of Enterobacteriaceae (0.375% versus 0.064%, P < 0.05), Staphylococcaceae families (0.049% versus 0.016%, P < 0.05) and Veillonella genus (0.096% versus 0.009%, P < 0.01) significantly decreased, whereas the abundance of Anaeroplasma genus (0.0005% versus 0.0178%, P < 0.01) significantly increased. The expression levels of NLRP3, ASC, and Caspase-1 were changed significantly (P < 0.05). In summary, the Chaihu-Shugan-San decoction modulated intestinal microbe dysbiosis, reduced fat accumulation, and alleviated inflammatory factor expression, which are all processes related to the NLRP3 inflammasome pathway in NAFLD rats.
研究领域:
药理学
·
微生物学
·
炎症研究
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-8
·
IF 5.479
Wang Shuangling,Yan Han,Wang Yalin,Wang Na,Lin Yulong,Li Meng
The peroxidase-like activity of hollow Prussian Blue nanocubes (hPBNCs) is used, in combination with the enzyme alcohol oxidase (AOx), in a colorimetric ethanol assay. Different from other nanozymes, the large cavity structure of the hPBNCs provides a larger surface and more binding sites for AOx to be bound on their surface or in the pores. This extremely enhances the sensitivity of the assay system. In the presence of ethanol, AOx is capable of catalyzing the oxidation of alcohols to aldehydes, accompanied by the generation of hydrogen peroxide (H 2 O 2 ). The hPBNCs act as peroxidase mimics and then can catalyze the oxidation of 3,3′5,5′-tetramethylbenzidine (TMB) by H 2 O 2 , resulting in a color change of the solution from colorless to blue with a strong absorption at 652 nm. The lower detection limit for ethanol is 1.41 μg∙mL −1 . Due to the high catalytic activity of hPBNCs in weakly acidic and neutral solutions, the system was successfully applied to the determination of ethanol in mice blood. This is critically important for studying the alcohol consumption and monitoring the ethanol toxicokinetics.
Schematic representation of hollow Prussian Blue nanocubes (hPBNCs) used as both a peroxidase mimetic and as a carrier for alcohol oxidase. Utilizing hPBNCs along with the ethanol conversion enzyme, a sensitive colorimetric assay for ethanol was developed and applied to blood samples with satisfactory results.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CELL PROLIFERATION
·
2020
·Vol. 53
·期 8
·e12871
·
IF 5.753
Hongping Yu,Pei Liu,Daoyu Zhu,Junhui Yin,Qianhao Yang,Yigang Huang,Yixuan Chen,Changqing Zhang,Youshui Gao
Objectives Osteonecrosis of the femoral head (ONFH), largely caused by alcohol abuse, is a refractory bone disease characterized by the impaired capacity of osteogenic differentiation of bone mesenchymal stem cells (BMSCs), as well as the disordered adipocyte accumulation. Chrysophanic acid (CPA) is a natural anthraquinone which has lipid regulation and bone protection capacity. The aim of this study was to reveal the potential function of CPA and the underlying mechanisms for the alcohol-induced ONFH. Materials and Methods The effects of alcohol and CPA on BMSCs were investigated by cell proliferation, induced differentiation assays and immunofluorescent staining. Meanwhile, the function of PI3K/AKT and AMPK pathway was investigated in the process of osteogenic and adipogenic differentiation, respectively. Furthermore, we established the rat model of alcohol-induced ONFH to reveal the pharmacotherapeutic effect of CPA in vivo using radiographical and histopathological methods. Results In vitro, alcohol significantly inhibited the proliferation and osteogenic differentiation of BMSCs but stimulated the adipogenic differentiation. However, CPA could counteract the anti-osteogenesis of alcohol partly via PI3K/AKT pathway and retard the promotion of alcohol-induced adipogenesis via AMPK pathway. In vivo, radiographical and histopathological findings showed that CPA could alleviate alcohol-induced ONFH and substantially restore the bone volume. Conclusions We demonstrated that CPA ameliorated alcohol-induced ONFH possibly via regulating the differentiation tendency of BMSCs. Hence, CPA may become a beneficial herb extract to alleviate alcohol-induced ONFH.
研究领域:
药理学
·
骨科
·
干细胞研究
✓ 已验证
DOI
PubMed
高影响因子
DESALINATION
·
2022
·
IF 11.211
Yuhua Xiong,Xingtao Zuo,Jiajie He,Juan Xiong,Cong Ma,Zhongbing Chen,Yun Zhou,Shuili Yu
High performance polyamide (PA) thin-film composite (TFC) membranes are in great demand. However, it is still a challenge to further enable PA TFC membranes to overcome the perm-selectivity tradeoff. Here, l -tartaric acid (LTA) and piperazine were first used as the aqueous phase monomers to fabricate LTA integrated PA (LTA/PA) TFC membranes via interfacial polymerization (IP) with trimesoyl chloride. The physicochemical property and separation performance of the PA TFC membranes were regulated via LTA concentrations. The introduction of LTA made the PA layer of the membrane thinner and smoother with enlarged pore size. The LTA/PA TFC membrane prepared with a higher LTA content had a maximal water flux (207.6 LMH/bar, 20 times higher than that of the control membrane), with superior rejections for the dyes (>98.0 %) and low removal rates for the salts (<4.5 %). Of note, the water flux of the membrane prepared with a low LTA content was enhanced to 18.9 LMH/bar without sacrificing the rejection of Na 2 SO 4 ( R Na 2 SO 4 > 98.0 %). The membranes with optimal LTA content break the tradeoff towards some dyes or salts, and presented superior performance compared with most other reported polymeric membranes. This work highlights the great potential of constructing natural acid-modulated PA composite membranes in water purification .
研究领域:
材料科学
·
膜技术
·
水净化
✓ 已验证
DOI
高影响因子
Hepatology Communications
·
2023
·
IF 5.1
Qian Du,Tingting Zhu,Guorong Wen,Hai Jin,Jiaxing An,Jingyu Xu,Rui Xie,Jiaxing Zhu,Xiaoxu Yang,Ting Zhang,Qi Liu,Shun Yao,Xingyue Yang,Biguang Tuo,Xiong Ma
Background: S100 calcium-binding protein A6 (S100A6) is a calcium-binding protein that is involved in a variety of cellular processes, such as proliferation, apoptosis, and the cellular response to various stress stimuli. However, its role in NAFLD and associated metabolic diseases remains uncertain. Methods and Results: In this study, we revealed a new function and mechanism of S100A6 in NAFLD. S100A6 expression was upregulated in human and mouse livers with hepatic steatosis, and the depletion of hepatic S100A6 remarkably inhibited lipid accumulation, insulin resistance, inflammation, and obesity in a high-fat, high-cholesterol (HFHC) diet-induced murine hepatic steatosis model. In vitro mechanistic investigations showed that the depletion of S100A6 in hepatocytes restored lipophagy, suggesting S100A6 inhibition could alleviate HFHC-induced NAFLD. Moreover, S100A6 liver-specific ablation mediated by AAV9 alleviated NAFLD in obese mice. Conclusions: Our study demonstrates that S100A6 functions as a positive regulator of NAFLD, targeting the S100A6-lipophagy axis may be a promising treatment option for NAFLD and associated metabolic diseases.
研究领域:
肝病学
·
代谢疾病
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Heming Luo,Yanfei Yang,Xia Zhao,Jianqiang Zhang,Yanzheng Chen
We report the direct synthesis of 3D sponge-like nanoporous carbons via carbonization of tartrate salt, including potassium tartrate and sodium tartrate, in inert atmosphere without any activation. The carbonization temperature is a very important parameter that ensures formation of the desired carbon structures and enhances their electrochemical performance in supercapacitors . Given that alkali metal is present in the selected carbon precursor, high-temperature activation causes reaming of carbon-K-800-1 to achieve a high specific surface area of 1018 m 2 g −1 , thereby rendering the material suitable for use as an electrode for supercapacitors. All of the aqueous measurements were performed in a three-electrode system with 6 M KOH as the electrolyte. Carbon-K-800-1 can deliver a high specific capacitance of 296 F g −1 (29.1 μF cm −2 ) at a constant charge–discharge current of 0.7 A g −1 ; this capacitance may be maintained at 213 F g −1 (20.9 μF cm −2 ) at 20 A g −1 . A long cycle stability of 95.1% capacitance retention was observed after 5000 charge–discharge cycles at a current density of 3 A g −1 .
研究领域:
材料科学
·
电化学
·
纳米科技
·
能源存储
·
功能材料
✓ 已验证
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 24
·4209
·
IF 5.1
Zihua Liang,Xiangchen Zhang,Shiwei Chen,Meiting Wang,Deying Men,Wangxin Liu,Xucong Lv
Inactivated Selenium-enriched yeast (YSe), as an organic source of selenium with multiple physiological activities, has attracted widespread attention. However, its potential to alleviate alcoholic liver injury (ALD) and its underlying mechanisms remain largely unexplored. This study explores the protective effects of inactivated YSe intervention on ALD in mice and clarifies its mechanism of action. The results indicated that, at the same selenium dose, inactivated YSe intervention was superior to inorganic selenium (sodium selenite) in alleviating ALD. Specifically, high-dose inactivated YSe significantly reduced the levels of serum ALT and AST in alcohol-exposed mice (38.69% and 24.67%, respectively), increased the level of HDL-C (16.83%), and effectively improved alcohol-induced lipid metabolism disorders and liver oxidative damage. At the same time, it significantly increased the concentration of short-chain fatty acids (SCFAs) in feces. 16S rRNA sequencing indicates that inactivated YSe intervention enhances the abundance of beneficial flora (such asBlautia,Oscillibacter,Anaerotruncus,Butyricicoccus, andRuminiclostridium) and simultaneously inhibits potentially harmful microbiota (such asxylanophium,Escherichia–Shigellaandoscilliumspirates) to restore the homeostasis of the intestinal microbiota in ALD mice. Liver metabolomics analysis revealed that inactivated YSe intervention significantly altered the liver metabolic profile. The core pathways that are regulated by YSe after alcohol disruption include glutathione metabolism, purine metabolism, riboflavin metabolism, etc. In conclusion, this study demonstrates that inactivated YSe can effectively alleviate ALD in mice by regulating the structure of the intestinal flora and restoring liver metabolic homeostasis, providing a scientific basis for its potential functional food component in the prevention and auxiliary management of ALD.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yanan Zhao,Bin Li,Hongting Deng,Chang Zhang,Yitong Wang,Lei Chen,Hui Teng
Prolonged and excessive intake of alcohol results in the onset of alcoholic liver disease, which is marked by oxidative stress, intestinal barrier dysfunction, and disturbance in the intestinal microbiome. Galangin, a potent flavonoid from Alpinia officinarum Hance, has been recognized for its diverse biological properties; however, its ability for protecting against alcohol-stimulated hepatotoxicity remains unexplored in prior research. In the current study, a Gao-Binge mouse model was established to assess the positive role and mechanisms of galangin upon alcohol-induced liver injury. The administration of galangin relieved liver pathological damage, oxidative stress, and NLRP3-mediated inflammation induced by alcohol. In addition, galangin significantly reversed abnormal intestinal histopathological manifestations and damaged the intestinal barrier function. Furthermore, microbiota composition revealed that galangin improved intestinal imbalance by improving the gut microbiota dysbiosis and short-chain fatty acid level. Collectively, this study explored the interactions between phytochemical factors and virulence factors and discovered that galangin powerfully improved alcohol-induced liver disease by repressing the inflammatory cascade via the gut microbiota-mediated gut–liver axis. These results suggested that alcohol-targeted natural products could have potential applications in promoting food safety and human health and offer valuable insights into the possible use of these substances in these important areas.
研究领域:
药理学
·
肝病学
·
微生物学
·
免疫学
·
营养学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）