Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Tong Feifei,Qin Zongmin,Wang Hongyue,Jiang Yingying,Li Junkuan,Ming Hui,Qu Ge,Xiao Yazhong,Sun Zhoutong
Chiral amino alcohols are prevalent synthons in pharmaceuticals and synthetic bioactive compounds. The efficient synthesis of chiral amino alcohols using ammonia as the sole amino donor under mild conditions is highly desired and challenging in organic chemistry and biotechnology. Our previous work explored a panel of engineered amine dehydrogenases (AmDHs) derived from amino acid dehydrogenase (AADH), enabling the one-step synthesis of chiral amino alcohols via the asymmetric reductive amination of α-hydroxy ketones. Although the AmDH-directed asymmetric reduction is in a high stereoselective manner, the activity is yet fully excavated. Herein, an engineered AmDH derived from a leucine dehydrogenase from Sporosarcina psychrophila (SpAmDH) was recruited as the starting enzyme, and the combinatorial active-site saturation test/iterative saturation mutagenesis (CAST/ISM) strategy was applied to improve the activity. After three rounds of mutagenesis in an iterative fashion, the best variant wh84 was obtained and proved to be effective in the asymmetric reductive amination of 1-hydroxy-2-butanone with 4-fold improvements in kcat/Km and total turnover number (TTN) values compared to those of the starting enzyme, while maintaining high enantioselectivity (ee >99%) and thermostability (T5015 >53°C). In preparative-scale reaction, the conversion of 100 and 200 mM 1-hydroxy-2-butanone catalyzed by wh84 was up to 91–99%. Insights into the source of an enhanced activity were gained by the computational analysis. Our work expands the catalytic repertoire and toolbox of AmDHs.
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Journal of Ocean University of China
·
2015
·Vol. 14
·期 4
·695-702
·
IF 0.558
Xu Houguo,Huang Lina,Liang Mengqing,Zheng Keke,Wang Xinxing
A 3-month feeding experiment was conducted in an in-door seawater system to investigate the effect of dietary vitamin E (Ve) on the sperm quality of turbot ( Scophthalmus maximus ). D-α-tocopherol acetate was supplemented to the basal (control) diet (65.14 mg kg −1 Ve) to obtain low and high levels of dietary Ve (244.60 mg kg −1 , LVe; 721.60 mg kg −1 , HVe). Compared with the control, sperm concentration was significantly increased in Ve-supplemented groups (LVe and HVe); while relative sperm volume and testis-somatic index were significantly increased in group HVe only. Sperm motility duration was significantly longer in group HVe than in the control, but no significant difference was observed in percent motility among groups. Sperm size, the uniformity of mitochondrial size, and the integrity of mitochondria cristae and plasma membrane were improved by dietary Ve, especially in HVe. The content of Ve in testis and liver as well as polyunsaturated fatty acids in sperm increased with dietary Ve. These results suggested that dietary Ve, especially at the high level (721.60 mg kg −1 ), significantly improved sperm concentration and motility duration and maintained normal sperm morphology of turbot.
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Advanced Composites and Hybrid Materials
·
2024
·Vol. 7
·期 5
·1-11
·
IF 23.2
Wen Xiaohong,Zhao Zengcai,Chen Yuchang,Shan Xinzhi,Zhao Xuefeng,Gao Xiumin,Zhuang Songlin
Multifunctional integrated flexible electronic skin (e-skin) is the essential medium for information exchange between humans and machines. Especially, the proximity/pressure/strain sensing has become a technological goal for various emerging wearable electronic devices, such as biomonitoring devices, smart electronics, augmented reality, and prosthetics. Herein, a stretchable hybrid electronic network-based e-skin is presented, fabricated by embedding 3D hollow MXene spheres/Ag NWs hybrid nanocomposite into PDMS, which can effectively avoid the electrode falling off due to stress concentration. This e-skin works in noncontact mode (proximity-negative capacitance) and contact mode (pressure-positive capacitance and strain-resistance) for multiplex detection of random external force stimuli without mutual interference. The macroscopic physical structure of stretchable electrodes and the microscopic hybrid three-dimensional conductive network jointly contribute to the good sensing performance of the device. This work provides an effective and universal strategy for the application of wearable intelligent electronic products that demand noncontact interaction and multimodal tactile perception.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xiaotian Xu,Shi-Fan Xue,Jiale Wang,Wei Zhang,Da Wu,Guoyue Shi,Min Zhang
Selective detection of phenolic compounds is imperative for environmental surveillance and public health safeguards. This study introduces a dual-electrode voltametric electronic tongue (e-tongue) leveraging transition metal oxide-nanoengineered laser-induced graphene (LIG) to resolve phenol and its cresol isomers (o-, m-, p-) despite overlapping electrochemical signals. Contrary to conventional single-electrode systems hindered by matrix-induced peak overlaps, our e-tongue employs a synergistic sensing arraying: MnO 2 /LIG and Bi 2 O 3 /LIG. These composites are fabricated via a one-step in situ laser doping process, ensuring uniform nanoparticle integration while mitigating distributional inhomogeneity and interfacial weaknesses associated with post-synthetic modifications. Both MnO 2 /LIG and Bi 2 O 3 /LIG exhibit augmented electroactive surface areas, conductivity, electron transport kinetics, hydrophilicity, and stability relative to pristine LIG. The dual-electrode system achieved robust differentiation of phenolics in buffered solutions, environmental water samples, and tobacco smoke matrices. This work pioneers a scalable nanoengineering strategy for multi-target analysis of structurally proximal pollutants, advancing point-of-need environmental monitoring technologies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yachao Zhang,Yan Gao,Xueli Zhang,Huiming Wang,Tian Xia,Congcong Bian,Shuang Liang,Xiaozhi Tang,Xia Wang
The amplification of the electrochemical signal is crucial to improving the sensing performance of an immunosensor. In this study, a signal amplification strategy based on the co-catalysis of horseradish peroxidase (HRP) and nanoporous gold (NPG) was used to fabricate an electrochemical immunosensor for the determination of Hepatitis B e antigen (HBeAg). For the resulting immunosensor, HRP catalyzed catechol and thus served as a signal label of secondary antibody. NPG with unique properties was selected as a carrier for capture antibody immobilization. Further, NPG had an efficient electrocatalytic activity towards catechol (the substrate of HRP), which thus significantly enhanced the electrochemical signal produced by the HRP-labeled secondary antibody. Based on the co-catalysis of HRP and NPG, the immunosensor exhibited a good linear relationship between peak current density and HBeAg concentration in a wide HBeAg detection range from 1 pg mL −1 to 1 ng mL −1 with a high sensitivity of 23.51 μA mL ng −1 cm −2 as well as a low detection limit of 0.064 pg mL −1 (S/N = 3). Additionally, the immunosensor presented a strong anti-interference capability against human serum, human serum/dopamine, human serum/tryptophan, human serum/NO 2 − , human serum/D-glucose, and human serum/IgG. For the detection of HBeAg in human serum, the concentrations detected by the immunosensor were in good agreement with the standard concentrations that added in the human serum samples. These unique properties potentially make the proposed immunosensor a good choice for HBeAg detection.
研究领域:
生物医学工程
·
分析化学
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BioNanoScience
·
2026
·Vol. 16
·期 5
·333
·
IF 4
Zhu Mingxue,Wei Lin,Han Xiangning,Li Guoqiang,Cao Limin,Wang Xiudan,Wang Kaiqiang,Lin Hong,Sui Jianxin
The preparation of specific antibodies is of critical importance in the immunoassay of small-molecule contaminants in food. However, due to the lack of immunogenicity, these small molecules must be conjugated to a carrier to form complete antigen in order to induce the generation of antibodies, and adjuvants are typically required during the immunization when traditional proteins were used as carriers. Therefore, identifying a substance that possesses both the characteristics of carrier and adjuvant is of significant research importance for antibody preparation. Outer membrane vesicles (OMVs) are nanoscale particles with a bilayer membrane and spherical structure produced by Gram-negative bacteria through natural budding. They contain various pathogen-associated molecular patterns (PAMPs), which could be recognized by pattern recognition receptors (PRRs) on the surface of immune cells, thereby triggering a robust immune response. In this study, OMVs derived from Δ lpxl E. coli and chloramphenicol sodium succinate (CSS) were used as the carrier and hapten to synthesize the complete chloramphenicol (CAP) antigen (CSS-Δ lpxl -OMV). BALB/c mice were subsequently immunized with this antigen to generate antibodies, with the aim of evaluating the adjuvant effects of Δ lpxl -OMV and its potential as a hapten carrier. The results indicated that Δ lpxl -OMV not only served as a carrier for binding the hapten to prepare the complete antigen but also exhibited significant adjuvant properties. The synthesized complete antigen possessed significant immunogenicity and could induce the mice to produce antibodies against the target without additional adjuvants.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Yuhao Zhu,Ruoyi Liu,Shuai Huang,Junjie Weng,Renfeng Gong,Ziqiang Xiang,Lie Chen,Yong Zhang,Kehong Lv,Jing Qiu,Ying Liu,Guanjun Liu,Qingshan Wu
Robotic grasping faces a fundamental trade-off between strong adhesion and easy, rapid release, particularly when handling fragile or heavy objects. While thermoresponsive tunable adhesives have been developed to decouple this conflict by enabling tunable adhesion via temperature changes, the lack of real-time tactile feedback limits adaptive control during dynamic manipulation. Inspired by the adhesive–tactile synergy observed in tree frog toe pads, we present a multifunctional electronic skin (e-skin) that integrates a temperature-regulated adhesive layer with a tactile perception layer for closed-loop manipulation. The adhesive layer, made of a PDMA-co-LMA (Poly(N,N-dimethylacrylamide-co-lauryl methacrylate)) copolymer, provides strong adhesion (143.46 kPa at 25 °C) and rapid release (5.41 kPa at 85 °C, a 96.23% reduction). The tactile layer, based on a clay-reinforced PDMA/ILs (ionic liquids) ionogel, exhibits a wide linear range (0–300 kPa, R2 = 0.998) and high sensitivity (13.129 kPa–1). Coupled with a real-time slip detection algorithm, the system achieves over 95% success in preventing slip and object damage. By merging tunable adhesion with tactile perception, this e-skin enables robust, adaptive, and nondestructive grasping in dynamic environments and provides a promising strategy for intelligent robotic grasping.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
He Yijing,Yang Yue,Wang Huixi,Zhang Fan,Cai Wentao,Yu Haixiang,Di Bin
Dextromethorphan (DXM) is a widely used cough suppressant,
and
its escalating abuse is a pressing global health concern that necessitates
rapid screening tools. However, rapid methods for detecting DXM in
complex matrices remain highly limited. Here, we report the first
isolation and engineering of DNA aptamers specifically targeting DXM.
Using library-immobilized SELEX, we identified high-affinity aptamers
( K D ∼ 1–2 μM) with
exceptional specificity against various interferents. The lead candidate
was rationally truncated into a minimal 27-nucleotide core (DM1–27)
that retains full function. Systematic mutagenesis and molecular modeling
further delineated the structure–activity relationship governing
target recognition. Utilizing this engineered aptamer, we developed
two distinct fluorescent platforms: a label-free thioflavin T displacement
assay and a fluorophore-quencher labeled hybrid beacon. Both sensors
enabled robust DXM quantification within 10 min in human saliva and
e-cigarette juice (EJ), achieving limits of detection at low ng/mL-level.
Notably, the hybrid beacon significantly improved matrix tolerance
by bypassing short-wavelength background interference. This work provides
a versatile molecular recognition tool and establishes practical,
field-deployable sensing strategies for DXM monitoring.
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Journal of Radiation Research and Applied Sciences
·
2025
·
IF 2.5
Lan Tang,Xin Zhang,Ye Huang,Depeng Kong,Yiwen Yan,Zhimin Ou,Mulan Zhu,Yi Li,Jianwei Wang
Objective This study aimed to enhance the solubility and dispersion of poorly water-soluble dexamethasone (DEX) in oral films by developing a novel pullulan-vitamin E succinate (PUL-VES) coupling compound (designated PUVS3) as the film matrix. Methods The amphiphilic coupling compound PUVS3 was synthesized, characterized and evaluated. Using PUVS3 as a solubilizing/suspension agent, and PUL as the film-forming material, a dexamethasone-fast dissolving oral film (DEX-FDOF) was prepared by solvent casting method. The film was evaluated for appearance, microstructure, mechanical properties, weight variation, content uniformity, disintegration time and in vitro dissolution. Results The DEX-FDOF formulation exhibited a smooth surface with no observable drug precipitation and demonstrated excellent transparency. Both weight variation and content uniformity complied with the pharmacopoeial standards. The film displayed rapid disintegration (<15s) and favorable mechanical properties with an elastic modulus of 35–45 MPa and elongation at break of 17%–20%. Notably, in vitro dissolution reached approximately 47 % within 10 min, representing a significant improvement over the pure drug. This enhancement was achieved through the use of a self-synthesized PUL-VES coupling compound as the film matrix. This innovative approach enabled homogeneous dispersion of the poorly water-soluble DEX within the water-soluble oral film while simultaneously improving its solubility characteristics. Conclusion The DEX-FDOF, fabricated using the self-synthesized PUL-VES coupling compound as the matrix, exhibited excellent uniformity, mechanical strength, rapid disintegration (<15 s), and notably enhanced dissolution (47 % in 10 min), demonstrating its potential as an effective delivery system for poorly soluble drugs such as DEX.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2026
·Vol. 27
·期 10
·4478
·
IF 5.6
You-Quan Ma,Lan Lin
Candida albicansis the causal agent of invasive candidiasis, which might be lethal in immunocompromised patients. Biofilm formation is considered a key virulence factor ofC. albicansand is associated with its elevated resistance to antifungals.C. albicansand bacteria likeE. coliare frequently found to form mixed biofilms on biotic or abiotic surfaces, rendering them more refractory to existing antifungals. To investigate howE. coliendogenous indole interplaying with exogenous IAA exerts modulatory effects on dual-species biofilm withC. albicans, anE. colistrain deficient in the indole biosynthetic genetnaAwas constructed, and the enzyme TnaA inhibitor was administered to block the indole production inE. colimonoculture and/orE. coli–C. albicansdual culture. Phenotypic assay revealed that indole deficiency attenuatedE. colimono-species biofilm by 12% (tnaA∆ versus WTE. coli), and the lack of indole in theE. colicell-free culture filtrate abolished the ability to promoteC. albicansbiofilms, evidenced by the fact that the treatment with WTE. coliculture supernatants exhibited a 1.7-fold promotive effect, while treatment withtnaA∆ displayed no significant difference from the broth control towardsC. albicansbiofilms. Furthermore, impairedE. coliindole production might disruptE. coli–C. albicansbiofilm, as examined by confocal laser scanning microscopy (CLSM). Moreover, indole-3-acetic acid (IAA) was found to exhibit more potent biofilm-modulatory activity than indole by CLSM imaging with dual biofilms of WTE. coli–C. albicans, in contrast to those ofE. coli tnaA∆–C. albicanspost-supplemented with exogenous IAA. This study provides evidence for indole as a signaling molecule mediating bacterial–fungal communication during mixed-biofilm formation. Indole and its derivatives, particularly in combination with existing antifungals, have potential in the development of anti-biofilm strategies to eradicate refractory fungal infections.
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JOURNAL OF MICROENCAPSULATION
·
2023
·
IF 3.9
Ran Tao,Qianyu Zhang,Jialing Duan,Ran Chen,Liyun Yao,Ruiteng Zhang,Gang Dong,Huali Chen
Aims The aim of this study is to develop a liposome that could exert unparalleled antioxidant effects. In the present study, a vitamin C (VC)/vitamin E (VE)-co-loaded multivesicular liposome (VCVE-MVL) was constructed.Methods Vitamins were encapsulated in soybean phosphatidylcholine (SPC) and cholesterol (CHO) by multi-emulsification method. The concentration of VC was determined by Fast Blue method. The concentration of VE was determined by high performance liquid chromatography (HPLC). Vitamin antioxidant capacity in vitro and in vivo was determined using β-carotene bleaching.Results VCVE-MVL with particle diameter of 848.55 ± 0.29 nm and SPAN of 0.16 ± 0.11 were obtained. The encapsulation efficiency of VC reached 48.51% (w/w)±0.15. Compared with VC/VE solution, VCVE-MVL had a higher permeation efficiency. In addition, the in vitro and ex-vivo antioxidant tests verified the adequate antioxidant activity of VCVE-MVL.Conclusions In conclusion, the antioxidant synergistic system we constructed and demonstrated its potential applications in the cosmetics industry.
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ARCHIVES OF MICROBIOLOGY
·
2023
·Vol. 205
·期 5
·1-10
·
IF 2.8
Guo Hui,Yang Wei,Lei Bingshuang,Zhao Fengju,Guo Lili,Qian Junqing
Food safety is a major public health concern all over the world. Therefore, the prevention of food contamination is becoming extremely crucial. In this study, an antimicrobial nanoemulsion composed of water-soluble nisin and fat-soluble octanoic acid was successfully prepared. The results showed that the average particle size and the polymer dispersity index of the nisin−octanoic acid (NOA) nanoemulsion were around 52.21 nm and 0.253, respectively. The NOA nanoemulsion required less amounts of nisin and octanoic acid to achieve the effective antimicrobial effect against Escherichia coli and Staphylococcus aureus . In addition, the growth curves of E. coli and S. aureus were determined. The OD 600 of NOA nanoemulsion was significantly lower than free nisin after being incubated for 24 h ( p < 0.001), indicating that the antimicrobial effect of NOA nanoemulsion was outstanding. Meanwhile, the synergistic antimicrobial property of NOA nanoemulsion against E. coli and S. aureus was significantly better than free nisin under nonacid conditions ( p < 0.05). Overall, the results of this study may provide guidance for the further application of nisin in more forms.
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