Frontiers in Genetics
·
2022
·
IF 4.772
Shan Mengjie,Liu Hao,Hao Yan,Song Kexin,Meng Tian,Feng Cheng,Wang Youbin,Huang Yongsheng
Background: Keloid is a skin fibroproliferative disease with unknown pathogenesis. Metabolomics provides a new perspective for revealing biomarkers related to metabolites and their metabolic mechanisms.Method: Metabolomics and transcriptomics were used for data analysis. Quality control of the data was performed to standardize the data. Principal component analysis (PCA), PLS-DA, OPLS-DA, univariate analysis, CIBERSORT, neural network model, and machine learning correlation analysis were used to calculate differential metabolites. The molecular mechanisms of characteristic metabolites and differentially expressed genes were identified through enrichment analysis and topological analysis.Result: Compared with normal tissue, lipids have a tendency to decrease in keloids, while peptides have a tendency to increase in keloids. Significantly different metabolites between the two groups were identified by random forest analysis, including 1-methylnicotinamide, 4-hydroxyproline, 5-hydroxylysine, and l-prolinamide. The metabolic pathways which play important roles in the pathogenesis of keloids included arachidonic acid metabolism and d-arginine and d-ornithine metabolism. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide are metabolic indicators of keloid severity. The high-risk early warning index for 5-hydroxylysine is 4 × 108-6.3×108 (p = 0.0008), and the high-risk predictive index for 1-methylnicotinamide is 0.95 × 107-1.6×107 (p = 0.0022).Conclusion: This study was the first to reveal the metabolome profile and transcriptome of keloids. Differential metabolites and metabolic pathways were calculated by machine learning. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide may be metabolic indicators of keloid severity.
研究领域:
皮肤病学
·
代谢组学
·
生物信息学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Simei Sun,Chaochao Qin,Hua Liu,Chao Jiang
Anthraquinone derivatives have been widely used as photocleavage agents and dyes. Here, photoinduced excited state intramolecular charge transfer (ICT) dynamics of 1-aminoanthraquinone in ethanol are studied by femtosecond transient absorption (fs-TA) spectroscopy and quantum chemical (QC) calculations. Four decay associated difference spectra and corresponding lifetime components were obtained by singular value decomposition and global fitting analysis from the fs-TA spectra. The QC calculations prove that the S 1 state has obvious ICT character. Planar ICT (PICT) and twisted ICT (TICT) reaction coordinates are observed. On the PICT coordinate, vibrational relaxation (VR) and intersystem crossing (ISC) processes are observed. The ISC efficiency is dependent on excitation wavelength, which elucidates that T′ 2 state participates in the ISC process on a higher-level than the S′ 1 state. On the TICT coordinate, the TICT process is dependent on excitation wavelength, which elucidates a direct experimental evidence of an energy barrier in excited state TICT potential energy.
研究领域:
化学
·
光化学
✓ 已验证
DOI
高影响因子
Journal of Superconductivity and Novel Magnetism
·
2025
·Vol. 38
·期 2
·112
·
IF 1.7
Li Xiaofeng,Gao Yuxia,Tian Zhaoming,Ren Weijie,Sun Xin,Wang Mingli
KNi(PO 3 ) 3 was successfully synthesized by solid-state method and the magnetic properties were characterized. The crystal structure of KNi(PO 3 ) 3 belongs to trigonal crystal system with space group R 3, where Ni 2+ ions form the distorted honeycomb-lattice within the ab-plane stacking in the “ABAB” type fashion along the c -axis. Magnetization measurements reveal the presence of antiferromagnetic (AFM) interaction with Curie Weiss (CW) temperature θ CW = − 12.905 K but without long-range magnetic order down to 2 K. Notably, the CW fitted effective moment µ eff = 3.35µ B and saturated magnetization M S = 2 μ B /Ni 2+ at 2 K support KNi(PO 3 ) 3 as an S = 1 spin system. Intriguingly, the zero-field specific heat exhibits a broad peak maximized at ~ 2.5 K indicating the onset of short-range spin correlation between Ni 2+ ions, while the integrated magnetic entropy ( S mag ) is close to the expected Rln3 for S = 1 system, indicative of large spin fluctuation below 2 K driven by spin frustration.
研究领域:
无机化学
·
固体化学
·
磁学
·
凝聚态物理
·
材料科学
·
晶体工程
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产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·Vol. 88
·期 6
·
IF 5.005
Ke Huang,Wei Liu,Yuanhe Li,Sha Zeng,Fang-Jie Zhao
Methylarsenate (MAs(V)) is a product of microbial arsenic (As) biomethylation and has also been widely used as an herbicide. Some microbes are able to reduce nontoxic MAs(V) to highly toxic methylarsenite (MAs(III)) possibly as an antibiotic. The mechanism of MAs(V) reduction in microbes has not been elucidated. Here, we found that the bacterium Enterobacter sp. CZ-1 isolated from an As-contaminated paddy soil has a strong ability to reduce MAs(V) to MAs(III). Using a MAs(III)-responsive biosensor to detect MAs(V) reduction in E. coli Trans5α transformants of a genomic library of Enterobacter sp. CZ-1, we identified gshA, encoding a glutamate-cysteine ligase, as a key gene involved in MAs(V) reduction. Heterologous expression of gshA increased the biosynthesis of glutathione (GSH) and MAs(V) reduction in E. coli Trans5α. Deletion of gshA in Enterobacter sp. CZ-1 abolished its ability to synthesize GSH and decreased its MAs(V) reduction ability markedly, which could be restored by supplementation of exogenous GSH. In the presence of MAs(V), Enterobacter sp. CZ-1 was able to inhibit the growth of Bacillus subtilis 168; this ability was lost in the gshA-deleted mutant. In addition, deletion of gshA greatly decreased the reduction of arsenate to arsenite. These results indicate that GSH plays an important role in MAs(V) reduction to generate MAs(III) as an antibiotic.IMPORTANCE Arsenic is a ubiquitous environmental toxin. Some microbes detoxify inorganic arsenic through biomethylation, generating relatively nontoxic pentavalent methylated arsenicals, such as methylarsenate. Methylarsenate has also been widely used as an herbicide. Surprisingly, some microbes reduce methylarsenate to highly toxic methylarsenite possibly to use the latter as an antibiotic. How microbes reduce methylarsenate to methylarsenite is unknown. Here, we show that gshA encoding a glutamate-cysteine ligase in the glutathione biosynthesis pathway is involved in methylarsenate reduction in Enterobacter sp. CZ-1. Our study provides new insights into the crucial role of glutathione in the transformation of a common arsenic compound to a natural antibiotic.
研究领域:
微生物学
·
环境科学
·
毒理学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 7
·3068
·
IF 4.6
Yuanyuan Ma,Wanting Liu,Zhifang Li,Yuhang Sun,Mingyuan Shi,Zheng Nan,Ruotong Song,Liying Wang,Jingqi Guan
TS-1 zeolite with desirable pore structure, an abundance of acidic sites, and good thermal stability promising as a support for the selective catalytic reduction of NO with NH3(NH3-SCR). Herein, a series of Mn–Fe/TS-1 catalysts have been synthesized, adopting tetraethylenepentamine (TEPA) as a metal complexing agent using the one-pot hydrothermal method. The introduced TEPA can not only increase the loading of active components but also prompts the formation of a hierarchical structure through decreasing the size of TS-1 nanocrystals to produce intercrystalline mesopores during the hydrothermal crystallization process. The optimized Mn–Fe/TS-1(R-2) catalyst shows remarkable NH3-SCR performance. Moreover, it exhibits excellent resistance to H2O and SO2at low temperatures. The characterization results indicate that Mn–Fe/TS-1(R-2) possesses abundant surface Mn4+and Fe2+and chemisorbed oxygen, strong reducibility, and a high Brønsted acid amount. For comparison, Mn–Fe/TiO2displays a narrower active temperature window due to its poor thermostability.Keywords:Mn-Fe/TS-1;one-pot synthesis;NH3-SCR;metal complexing;resistance to H2O and SO2
研究领域:
催化化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2013
·
IF 3.187
Sheng-Jie Chen,Yong-Yong Wang,Wei-Min Yao,Xiao-Li Zhao,Giang VO-Thanh,Ye Liu
A novel ionic complex with insensitivity to moisture and air, bis-[1-butyl-2-diphenylphosphanyl-3-methylimidazolium]tetrachloridorhodium(III) hexafluorophosphate ( 1A ), has been synthesized and fully characterized. The single-crystal X-ray diffraction analysis shows that 1A is composed of the Rh(III)-complex cation and the counter-anion of PF 6 − . The complex cation has highly symmetrical Rh III -centered octahedron geometry with four Cl atoms in the equatorial plane and two imidazolium-substituted phosphine ligands in the axial position. The catalytic performance of 1A , which could be tailored by the ligands and the solvents, was investigated toward the biphasic hydroformylation of 1-octene. It was found that the presence of the hemilabile hybrid P,N-ligand of 3 (1-(2′-piperid-1′-yl-ethyl)-2-diphenylphosphino-3-methylimidazolium hexafluorophosphate), as a kind of ligand-functionalized ionic liquids (ILs), could dramatically spur the catalytic activity of 1A . The use of the RTIL of [PEmim]BF 4 (1-(2′-piperid-1′-yl-ethyl)-3-methylimidazolium tetrafluoroborate) as the solvent not only fulfilled the efficient biphasic hydroformylation of 1-octene, but also guaranteed the successful recovery and recycling of the ionic compositions of 1A and 3 .
研究领域:
化学
·
催化反应
✓ 已验证
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Meichen Liu,Meichen Gao,Xu Shi,Yilin Yin,Huanyi Liu,Ruirui Xie,Chenxi Huang,Wenwen Zhang,Shiwen Xu
With the increasing development of the society, silicon dioxide (SiO 2 ) has been used in various fields, such as agriculture, food industry, etc., and its residues can pose a potential health threat to organisms. Quercetin (Que) is a potent free radical scavenger commonly found in plants. C57BL/6 mice were chosen to established a mouse model of SiO 2 exposure and Que antagonism to investigate the mechanism of action of Que in rescuing the toxic damage of SiO 2 on mouse cerebellum tissue. The results showed that cytoplasmic vacuolization, and inflammatory cell infiltration caused by SiO 2 were alleviated by the addition of Que, and reduced oxidative stress in mouse cerebellum, alleviated the activation of TLR4 pathway induced by SiO 2 , and substantially reduced the occurrence of ZBP-1-mediated PANoptosis induced by SiO 2 exposure in mouse cerebellum. In NS20Y cells, the oxidative stress activator (Elesclomol) and inhibitor N-acetyl cysteine (NAC), and the NF-κB activator 2 (NA2) were added. Elesclomol and NAC confirm the involvement of ROS in regulating the TLR4/NF-κB pathway, the TLR4/NF-κB pathway regulated ZBP-1-mediated PANoptosis in cerebellum and NS20Y cells induced by SiO 2 exposure. In conclusion, the present experimental data suggest that Que mitigates the onset of ZBP-1-mediated PANoptosis in neuronal cells induced by SiO 2 through the ROS/TLR4/NF-κB pathway. The present experimental findings help to understand the detoxification effect of Que in more tissues and provide an important reference for the rescue of organisms in long-term SiO 2 environment.
研究领域:
神经生物学
·
毒理学
·
细胞信号传导
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Jun-Sheng Liu,Yue-Xiang Zeng,Shi-Yuan Bi,Jin-Wei Zhou,Rui Cheng,Jing Li,Ai-Qun Jia
Phomopsis liquidambari is a classical endophytic fungus with great application potential in ecology and agriculture; however, studies on its exopolysaccharides are lacking. Here, we aimed to evaluate the structure and bioactivity of PLN-1, an exopolysaccharide derived from the P. liquidambari NJUSTb1 strain. The structure was elucidated by chromatography/spectral methods and hydrolyzation. Immunomodulation, moisture absorption, and retention properties were investigated after sulfation and carboxymethylation modification. Results showed that PLN-1 contained a linear repeating unit of →[4)- α - d -Glc p -(1→6)- α - d -Glc p -(1→4)- α - d -Glc p -(1→4)- α - d -Glc p -(1→] n , with a molecular weight of 343 kDa. The degrees of substitution of sulfated polysaccharide (S-PLN-1) and carboxymethylated polysaccharide (C-PLN-1) were 1.228 and 0.903, respectively. S-PLN-1 showed stronger moisture absorption and retention properties than PLN (crude EPS), C-PLN1, and PLN-1. Furthermore, PLN, S-PLN-1, and C-PLN-1 stimulated the proliferation of RAW 264.7 cells with no cytotoxicity. The elucidation of PLN-1 in this study paves the way for future applications.
研究领域:
微生物学
·
生物化学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
IONICS
·
2020
·Vol. 27
·期 2
·657-666
·
IF 2.394
Hu Guorong,Liu Yanhua,Li Wei,Peng Zhongdong,Wu Jiahui,Xue Zhichen,Shi You,Fan Ju,Sun Qian,Cao Yanbing,Du Ke
P2-type Na 0.67 Ni 0.25 Mn 0.75 O 2 is considered as one of the most promising cathode materials for sodium-ion batteries due to its high initial capacity and operating voltage based on the Ni 2+ /Ni 4+ redox reaction. However, it suffers from rapid capacity decay caused by P2–O2 phase transition when the cutoff voltage is above 4.2 V (vs. Na + /Na). Herein, a high-voltage phase transition from P2 to an amorphous phase with poor crystallinity has been proved to be the main cause of material deterioration and Mn redox process is another cause. Cu-substituted Na 0.67 (Ni 0.25 Mn 0.75 ) 1−x Cu x O 2 ( x = 0, 0.05, 0.1, 0.2) with active redox couples of Ni 2+ /Ni 4+ and Cu 2+ /Cu 3+ has been fabricated for solving these issues. It is proved that Cu substitution can reduce the Mn 3+ /Mn 4+ reaction below 3 V (vs. Na + /Na) and suppress the high-voltage phase transition effectively at deep de-sodiated state, resulting in the significantly enhanced cycle life and rate capability of Na 0.67 Ni 0.25 Mn 0.75 O 2 .
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
Nano Research
·
2023
·
IF 9.9
Liu Yang,He Xuwei,Lian Zewei,Guo Qian,Chi Jimei,Lin Xiaoxue,Zhang Liyue,Liu Zheng,Liu Yingyuan,Su Meng,Wang Keyu,Ao Qiangguo,Cheng Qingli
Urinary kidney injury molecule 1 (uKIM-1) serves as a reliable marker for the early diagnosis of acute kidney injury (AKI). The rapid and facile detection of changes in uKIM-1 is essential for early AKI diagnosis, ultimately improving the prognosis of patients. In this study, we developed a fully printed photonic crystal-integrated microarray with photonic crystal-enhanced fluorescence properties, which can detect uKIM-1 levels at the point-of-care. We confirmed its efficacy in the early diagnosis of AKI using clinical urine specimens. Direct quantitative detection of uKIM-1 was achieved within 10 min. The lowest limit of detection is 8.75 pg·mL −1 with an accuracy of 94.2%. The diagnostic efficacy was validated using 86 clinical urine samples, highlighting the high sensitivity and stability of the photonic crystal microarray. Consequently, a facile and reliable immunoassay was designed and prepared for the rapid quantitative detection of uKIM-1, which is crucial for the early identification and convenient detection of AKI in hospital or community settings. Rapid, convenient, cost-effective, and long-term monitoring of changes in uKIM-1 levels can assist clinicians in making timely adjustments to treatment regimens, preventing the transition from AKI to chronic kidney disease (CKD), improving the quality of life of patients with AKI, and reducing healthcare costs. It highlights the advantages of utilizing urine samples as a noninvasive and easily accessible medium for early detection and monitoring of kidney-related conditions.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2017
·Vol. 27
·期 1
·101-109
·
IF 1.577
Li Qian,Liu Li,Zhong Xin-Xin,Li Fa-Bao,Asiri Abdullah M.,Alamry Khalid A.,Zhu Nian-Yong,Wong Wai-Yeung,Qin Hai-Mei
Abstract A series of neutral luminescent tetranuclear and mononuclear copper(I) halide complexes, [Cu 4 I 4 (dpna) 4 ] ( 1 ) and [CuX(dpna) 2 ] dpna = 1-(diphenylphosphino)naphthalene, X = Br ( 2 ), Cl ( 3 ), were synthesized, and their molecular structures and photophysical properties were investigated. Complex 1 has a cubane-like structure with four Cu atoms pseudo -tetrahedral and four I atoms serving as μ 3 -bridges. The coordination geometry of the copper centers in 2–3 are trigonal-planar. In the solid state, complexes 1–3 display blue photoluminescence (λ max = 423–463 nm) at 298 K. The emission of the complexes originate from the (σ+X)→π* transition. All three complexes displayed good thermal stability. Graphical Abstract
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产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 45
·5372-5379
·
IF 2.073
Odoom Jibrael Kingsford,Junjuan Qian,Depeng Zhang,Yinhui Yi,Gangbing Zhu
The occurrence of 1-chloro-4-nitrobenzene (CNB) in the environment due to increased agricultural and economic activities worldwide has been a serious concern for various environmental agencies; hence the qualitative and quantitative determination of CNB is of great significance. In this study, we took into consideration the features of carbon nanohorns (CNHs), such as high surface area and electron transfer ability, and β-cyclodextrin (β-CD), such as high host–guest recognition ability with a hydrophilic outer layer, to design a highly sensitive electrochemical sensor for detecting CNB by preparing CNHs and β-CD nanohybrids through a simple ultrasonication process. The outcome of our study revealed that the CNHs/β-CD nanohybrid modified electrode is an excellent electrochemical sensor for CNB. Under the optimized conditions, the experimental results show a considerable linear response range, unsubstantial interference from familiar organic and inorganic compounds and a detection limit of 9.0 nM.
研究领域:
环境科学
·
分析化学
·
纳米科技
·
电化学
·
污染物检测
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）