FOOD CHEMISTRY
·
2024
·
IF 8.5
Na Li,Junren Xu,Yiqiao Zhao,Mengmeng Zhao,Zhonghua Liu,Kunbo Wang,Jianan Huang,Mingzhi Zhu
Tea polyphenols transform under processing methods, but a systematic study on their changes in the same large-leaf tea cultivar is lacking. Here, Camellia sinensis var. assamica cv. Yunkang-10 leaves underwent six processing methods and were assessed using optimized nontargeted (UHPLC-Q-Exactive Orbitrap-MS) and targeted (UHPLC-QqQ-MS) polyphenomics, along with molecular networking analysis. 903 and 52 polyphenolic compounds (catechins, flavones and flavonols, and phenolic acids) were respectively relatively and absolutely quantified for the first time. Dark and black teas, with the lowest polyphenol content, differed from the other four tea types, although variations existed among these four teas. However, some flavonol and flavone aglycones (e.g. kaempferol, apigenin), as well as some phenolic acids (e.g. ellagic acid, gallic acid), exhibited higher levels in dark and black teas. Correlations between polyphenolic composition and electronic sensory characteristics were observed using E-tongue and E-eye. This study enriches understanding of polyphenol profiles in Chinese teas post diverse processing.
研究领域:
食品科学
·
植物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Enming Ping,Xiaoxiao Chen,Yunshan Zhou,Lijuan Zhang,Lingyun Kong,Nan Chen
In this work, CAU-10-H@γ-AlOOH is prepared, and then UTSA-16 is loaded on CAU-10-H@γ-AlOOH to obtain UTSA-16@CAU-10-H@γ-AlOOH. Using the as-prepared composites as stationary materials by cryogenic gas chromatography at 77 K, while CAU-10-H@γ-AlOOH achieves the complete separation of ortho-H2 (o-H2) and D2 with a resolution R of 1.66 and a separation time t of 9.52 min, UTSA-16@CAU-10-H@γ-AlOOH achieves higher efficiency separation of hydrogen isotopes in a shorter separation time (4.56 min) with R = 1.7. Molecular simulation results show that CAU-10-H has both chemical affinity quantum sieving and kinetic sieving effects for H2/D2 at 77 K, and UTSA-16 can only exert the kinetic sieving effect. UTSA-16’s load on CAU-10-H@γ-AlOOH weakens the adsorption of hydrogen isotopes, and the presence of Co2+ in UTSA-16 promotes the conversion of para-H2 to ortho-H2. In gas chromatography, H2 was preferentially desorbed from the system due to strong D2 adsorption caused by the chemical affinity quantum sieving effect and faster H2 diffusion caused by the kinetic sieving effect. These additive effects achieved efficient hydrogen isotope separation at 77 K.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
·
2018
·Vol. 42
·期 6
·3093-3104
·
IF 2.784
Yang Zhou,Tao Hong,Li Tong,Wei Liu,Xueting Yang,Jianghan Luo,Fuling Wang,Jian Li,Lijun Yan
Non‑small cell lung carcinoma (NSCLC) is a life‑threatening malignancy. The level of the cell growth regulator mitogen‑activated protein kinase kinase kinase kinase 3 (MAP4K3) has been shown to be correlated with a high risk of NSCLC recurrence and poor recurrence‑free survival rate. The present study examined the effects of Astragalus polysaccharide (APS) and 10‑hydroxycamptothecin (HCPT), which are associated with marked suppression and dephosphorylation of the MAP4K3/mammalian target of rapamycin (mTOR) signaling pathway, in the H1299 NSCLC cell line. APS and HCPT decreased H1299 cell viability, induced apoptosis and altered the cell cycle stages, as evaluated using an 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay and flow cytometric analysis. Furthermore, APS increased the expression of apoptosis‑associated genes B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein (BAX), of proteases cysteine‑aspartic acid protease (caspase)‑3 and ‑9, and of cytochrome c. HCPT promoted autophagy in H1299 cells, with concomitant suppression of the expression of MAP4K3 and downregulation of mTOR signaling. Notably, combination treatment with the two agents reduced the migration and invasion of H1299 cells compared with the single treatments. It was also demonstrated that the overexpression of MAP4K3 promoted the migration and invasion of H1299 cells, and that the kinase activity was essential to this. These findings suggested that MAP4K3 may be an attractive target for the treatment of NSCLC.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Chemical Neuroscience
·
2021
·
IF 4.418
Zhenquan Xuan,Xinmei Gu,Sicheng Yan,Yanfei Xie,Yiying Zhou,Hui Zhang,Haibo Jin,Shengquan Hu,Marvin S. H. Mak,Dongsheng Zhou,Karl Wah Keung Tsim,Paul R. Carlier,Yifan Han,Wei Cui
Alzheimer’s disease (AD) is a neurodegenerative disorder with multiple pathological features. Therefore, a multitarget-directed ligands (MTDLs) strategy has been developed to treat AD. We have previously designed and synthesized dimeric tacrine(10)-hupyridone (A10E), a novel tacrine derivative with acetylcholinesterase (AChE) inhibition and brain-derived neurotrophic factor (BDNF) activation activity, by linking tacrine and a fragment of huperzine A. However, it was largely unknown whether A10E could act on other AD targets and produce cognitive-enhancing ability in AD animal models. In this study, A10E could prevent cognitive impairments in APP/PS1 transgenic mice and β-amyloid (Aβ) oligomers-treated mice, with higher potency than tacrine and huperzine A. Moreover, A10E could effectively inhibit Aβ production and deposition, alleviate neuroinflammation, enhance BDNF expression, and elevate cholinergic neurotransmission in vivo. At nanomolar concentrations, A10E could inhibit Aβ oligomers-induced neurotoxicity via the activation of tyrosine kinase receptor B (TrkB)/Akt pathway in SH-SY5Y cells. Furthermore, Aβ oligomerization and fibrillization could be directly disrupted by A10E. Importantly, A10E at high concentrations did not produce obvious hepatotoxicity. Our results indicated that A10E could produce anti-AD neuroprotective effects via the inhibition of Aβ aggregation, the activation of the BDNF/TrkB pathway, the alleviation of neuroinflammation, and the decrease of AChE activity. As MTDLs could produce additional benefits, such as overcoming the deficits of drug combination and enhancing the compliance of AD patients, our results also suggested that A10E might be developed as a promising MTDL lead for the treatment of AD.
研究领域:
神经科学
·
药理学
·
分子生物学
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Haifeng Lu,Lichao Xu,Hua Wang,Shengyu Feng
Fluorescent silicone polymers have attracted much attention owing to their strong fluorescence and high stabilities. However, most of these silicone polymers have suffered from fluorescence quenching caused by aggregation in high concentrations. In this work, 7, 11-diphenyl-8 H - acenaphth[1, 2 - f ] isoindole-8, 10 (9 H ) - dione functionalized polysiloxane (PMDF) with strong blue fluorescence was successfully synthesized via Diels-Alder reaction. Besides high thermal stability, PMDF showed unique aggregation-induced emission enhancement in high concentrations, indicating it is an excellent fluorescent silicone polymer in both disperse and aggregate state. Moreover, the product polymer showed highly sensitive fluorescence response to Fe 3+ , with low detection limit to 0.895 ppm and a high selectivity in the present of other metal ions. This work may open a new window for exploring fluorescent polymers as ideal probe for Fe 3+ .
研究领域:
材料科学
·
分析化学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
CNS Neuroscience & Therapeutics
·
2021
·Vol. 27
·期 10
·1157-1172
·
IF 5.243
Le Ma,Shiyu Peng,Jinbao Wei,Mengjing Zhao,Khalil Ali Ahmad,Jinghong Chen,Yong-Xiang Wang
Aim This study aimed to investigate the regulation of pain hypersensitivity induced by the spinal synaptic transmission mechanisms underlying interleukin (IL)-10 and glucagon-like peptide 1 receptor (GLP-1R) agonist exenatide-induced pain anti-hypersensitivity in neuropathic rats through spinal nerve ligations. Methods Neuropathic pain model was established by spinal nerve ligation of L5/L6 and verified by electrophysiological recording and immunofluorescence staining. Microglial expression of β-endorphin through autocrine IL-10- and exenatide-induced inhibition of glutamatergic transmission were performed by behavioral tests coupled with whole-cell recording of miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) through application of endogenous and exogenous IL-10 and β-endorphin. Results Intrathecal injections of IL-10, exenatide, and the μ-opioid receptor (MOR) agonists β-endorphin and DAMGO inhibited thermal hyperalgesia and mechanical allodynia in neuropathic rats. Whole-cell recordings of bath application of exenatide, IL-10, and β-endorphin showed similarly suppressed enhanced frequency and amplitude of the mEPSCs in the spinal dorsal horn neurons of laminae II, but did not reduce the frequency and amplitude of mIPSCs in neuropathic rats. The inhibitory effects of IL-10 and exenatide on pain hypersensitive behaviors and spinal synaptic plasticity were totally blocked by pretreatment of IL-10 antibody, β-endorphin antiserum, and MOR antagonist CTAP. In addition, the microglial metabolic inhibitor minocycline blocked the inhibitory effects of IL-10 and exenatide but not β-endorphin on spinal synaptic plasticity. Conclusion This suggests that spinal microglial expression of β-endorphin mediates IL-10- and exenatide-induced inhibition of glutamatergic transmission and pain hypersensitivity via presynaptic and postsynaptic MORs in spinal dorsal horn.
研究领域:
神经科学
·
药理学
·
疼痛研究
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Zhang Yuan,Zhou Xiaomeng,Zhang Nian,Zhu Jiaqi,Bai Ningning,Hou Xingyu,Sun Tao,Li Gang,Zhao Lingyu,Chen Yingchun,Wang Liu,Guo Chuan Fei
Flexible pressure sensors can convert mechanical stimuli to electrical signals to interact with the surroundings, mimicking the functionality of the human skins. Piezocapacitive pressure sensors, a class of most widely used devices for artificial skins, however, often suffer from slow response-relaxation speed (tens of milliseconds) and thus fail to detect dynamic stimuli or high-frequency vibrations. Here, we show that the contact-separation behavior of the electrode-dielectric interface is an energy dissipation process that substantially determines the response-relaxation time of the sensors. We thus reduce the response and relaxation time to ~0.04 ms using a bonded microstructured interface that effectively diminishes interfacial friction and energy dissipation. The high response-relaxation speed allows the sensor to detect vibrations over 10 kHz, which enables not only dynamic force detection, but also acoustic applications. This sensor also shows negligible hysteresis to precisely track dynamic stimuli. Our work opens a path that can substantially promote the response-relaxation speed of piezocapacitive pressure sensors into submillisecond range and extend their applications in acoustic range.
研究领域:
材料科学
·
电子学
·
传感器
·
生物医学工程
·
机械工程
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xinyan Wang,Yuting Zhang,Jian Zhang,Chengpeng Shao,Jiaxing Yang,Yuning Zhang,Ruoyi Li,Zheng Xing
In this study, a Z-type heterojunction photocatalyst CoWO 4 /CS/BiOCl-10 was constructed by hydrothermal synthesis for the efficient removal of norfloxacin (NOR) contaminants in water. X-ray photoelectron spectroscopy (XPS) combined with Fourier transform infrared spectroscopy (FT-IR) confirmed that the hydroxyl group in the chitosan (CS) molecule was bonded with the oxygen atom on the surface of CoWO 4 through hydrogen bonding to form a directional charge transport channel, which greatly improved the photocatalytic degradation efficiency of CoWO 4 /CS/BiOCl-10, and the degradation rate was as high as 98.30 % within 90 min under 300 W xenon lamp irradiation. Optoelectronic measurements show that the interfacial potential difference drives electrons to migrate along the Z-path. Density functional theory (DFT) and liquid chromatography-mass spectrometry (LC-MS) were used to identify intermediates in the NOR degradation process, revealing the degradation pathway of NOR through piperazine ring cleavage and defluorination. The kinetics of NOR degradation intermediates were revealed using two-dimensional correlation spectroscopy (2DCOS) techniques. Rice seed germination experiments have shown that the degraded NOR solution is virtually non-toxic, greatly reducing the environmental impact. This study provides a new strategy for the development of efficient photocatalytic systems through the synergistic regulation mechanism of hydrogen bonding and heterojunction.
研究领域:
环境科学
·
材料科学
·
光催化
·
水处理
·
化学工程
·
光谱学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2011
·Vol. 22
·期 6
·2632-2640
·
IF 5.101
Daqin Chen,Yunlong Yu,Feng Huang,Hang Lin,Ping Huang,Anping Yang,Zhaoxing Wang,Yuansheng Wang
We present a novel strategy involving the lanthanide dopant-induced formation of ultrasmall (sub-10 nm) uniform Ln3+:BaF2/Ln3+:SrF2 active-core/active-shell architectures. The lanthanide ions doped in the shell are demonstrated to play a key role to retard the growth of the core/shell nanocrystals. Particularly, adopting ∼3 nm Tm3+,Yb3+:BaF2 nanocrystals as cores prepared by a solvothermal reaction, growth of Gd3+,Nd3+:SrF2 shells is successfully induced on the surfaces of these cores through a thermal decomposition process, forming ∼7 nm highly uniform and monodisperse Tm3+,Yb3+:BaF2/Gd3+,Nd3+:SrF2 active-core/active-shell nanocubes. In this architecture, the Gd3+,Nd3+:SrF2 shell not only benefits the enhancement of the near-infrared to near-infrared upconversion luminescence of the Tm3+,Yb3+:BaF2 core, but also acts as the host to realize the near-infrared to near-infrared downconversion luminescence of Nd3+ dopants and the paramagnetism of Gd3+ ones. Importantly, the doping of Tm3+/Yb3+ and Nd3+ into the core and shell respectively effectively suppresses the adverse energy transfer from Tm3+ to Nd3+ as well as from Nd3+ to Yb3+, resulting in lessening of the quenching for both Tm3+ upconversion and Nd3+,Yb3+ downconversion emissions. These hydrophobic core/shell nanocrystals are further converted into hydrophilic ones using thioglycolic acid as the surface ligand. The sub-10 nm water-soluble active-core/active-shell architectures with near-infrared to near-infrared dual-modal luminescence and proper paramagnetism may find potential applications in biomedical imaging and detection.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
光学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 24
·10873-10883
·
IF 2.7
Yunxia Shi,Hui Duan,Meiyan Du,Xia Kong,Ke Xue,Guang Lu,Qingyun Liu
In this work, 2(3),9(10),16(17),23(24)-octamethoxyphthalocyanine (Pc(OH)8) was first used to modify Cu/CuFe2O4(Cu/CFO) nanospheres, and Pc(OH)8–Cu/CFO nanocomposites with enhanced peroxidase mimetic enzyme activity was obtained compared to the performance of Cu/CFO alone. SEM, XRD, and XPS were used to study the surface morphology, phase composition and elemental analysis of the newly synthesized Pc(OH)8–Cu/CFO. Using TMB as the chromogenic substrate, Pc(OH)8–Cu/CFO accelerate the oxidation process of chromogenic substrate 3,3′,5,5′- tetramethylbenzidine (TMB) by hydrogen peroxide in three minutes. The catalytic oxidation mechanism of Pc(OH)8–Cu/CFO on TMB was comprehensively investigated through free radical and fluorescence probe experiments. Based on the excellent catalytic performance of the composite materials, a simple and fast colorimetric sensing platform for the colorimetric detection of H2O2 and glutathione (GSH) was constructed in the range of 0.6–5 mM and 2–10 μM with detection limits (LODs) of 0.35 mM and 0.55 μM, respectively. In addition, using Pc(OH)8–Cu/CFO colorimetric detection for GSH has a lower detection limit, good selectivity and high sensitivity, which is expected to become an ideal material for GSH colorimetric detection.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
BIOPHYSICAL CHEMISTRY
·
2021
·
IF 2.352
Yuyan Guo,Tao Gao,Fang Fang,Shuang Sun,Dayu Yang,Yongji Li,Shaowa Lv
A novel polyethylene glycol-polycaprolactone-poly- l -tyrosine (MPEG-PCL-PTyr) amphiphilic triblock copolymer micelle was synthesized for the first time. 10-hydroxycamptothecin (HCPT) was embedded in MPEG-PCL-PTyr nanomicelles using the emulsion solvent evaporation method. A series of was conducted to confirm the structure of the compound and to evaluate the physical properties of the MPEG-PCL-PTyr nanomicelles. Cellular uptake, cytotoxicity, and apoptosis were assessed using flow cytometry and MTT assays. Confocal microscopy and flow cytometry results demonstrated that the nanocapsules carrying HCPT had significantly increased anti-tumor activity against HepG2 cells and could target HepG2 cell lysosomes with obvious liver targeting. In addition, the drug-loaded nanomicelles could significantly block the S phase of cancer cells and induce apoptosis; thus, they could be potential carriers for future 10-HCPT delivery and cancer treatment.
研究领域:
药学
·
纳米技术
·
肿瘤学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 19
·9931-9946
·
IF 6.317
Xiyi Hu,Zhenguo Liu,Yuntao Lu,Xuepeng Chi,Kai Han,Hongfang Wang,Ying Wang,Lanting Ma,Baohua Xu
10-Hydroxy-2-decenoic acid (10-HDA) is a principal active ingredients of royal jelly. Several recent studies demonstrated that 10-HDA has potential anti-type 2 diabetes mellitus (T2DM) properties. To evaluate the anti-T2DM effect of 10-HDA and explore its underlying molecular mechanisms, we used high fat diet (HFD) combined with streptozotocin (STZ) injection to establish a diabetes model. Mice were randomly divided into four groups (8 mice per group): control group, 10-HDA group, T2DM group, and T2DM + 10-HDA group. The 10-HDA and T2DM + 10-HDA groups were administered intragastric 10-HDA (100 mg per kg body weight), while the control and T2DM groups were administered a vehicle, daily for 4 weeks. Our analysis indicated that there was no significant difference in body weight between T2DM + 10-HDA and control group mice (P > 0.05). Treatment with 10-HDA reduced fasting blood glucose and increased insulin levels in diabetic mice (P < 0.05), as well as increasing the area of pancreatic islets (P < 0.05), and alleviating vacuolar degeneration in the liver. Further, 10-HDA intervention increased superoxide dismutase, catalase, and glutathione peroxidase activities in diabetic mouse liver, alleviated lipid peroxidation, inhibited liver NF-κB nuclear translocation, decreased IL-6 and TNF-α content, and increased P-PI3K, P-AKT, and P-GSK3β protein levels (all P < 0.05). Fifteen potential biomarkers were screened by analysis of liver metabolomics data, of which hexadecanamide, stearamide, pentadecanoic acid, and fatty acid esters of hydroxy fatty acids (16:0/18:1) were highly abundant. In conclusion, 10-HDA has clear hypoglycemic effects on diabetic mice, through the PI3K/AKT/GSK3β signaling pathway.
研究领域:
药理学
·
内分泌学
·
代谢学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）