Journal of Materials Chemistry B
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2021
·Vol. 9
·期 19
·4039-4049
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IF 6.331
Hao Pan,Changcan Shi,Rongshuai Yang,Guanghui Xi,Chao Lu,Xuanxin Yang,Jie Chen,Xiaojie Wang,Lei Chen,Jingye Pan
Keratinocyte growth factor-2 (KGF-2) can regulate the proliferation and differentiation of keratinocyte, which plays a remarkable role in maintaining normal tissue structure and promoting wound healing. As an effective strategy, KGF-2 solution is widely used in the treatment of wounds in clinical applications. However, KGF-2 in solution cannot achieve sustained release, which results in drug loss and unnecessary waste. Polysaccharide hemostasis microspheres (PHMs) are an ideal drug loading platform due to their special “lotus seedpod surface-like” morphology and structure. Herein, to realize the controllable release of KGF-2, PHMs loaded with KGF-2 (KGF-2@PHMs) were prepared. It was found that the bioavailability of KGF-2 was improved greatly. Most importantly, KGF-2@PHMs can reduce inflammation and accelerate the wound healing process due to the controlled release of KGF-2. KGF-2@PHMs might be a potential alternative strategy for wound healing in future clinical applications.
研究领域:
药剂学
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生物医学工程
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组织工程
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伤口愈合
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DOI
PubMed
高影响因子
TALANTA
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2017
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IF 4.162
Ke Mao,Haiqin Du,Longchao Bai,Yalin Zhang,Haikun Zhu,Yanmei Wang
In this work, a one-step coating procedure by a simple annealing protocol of poly (2-methyl-2-oxazoline)-random-glycidyl methacrylate (PMOXA-r-GMA) copolymer was used to yield covalent and cross-linked PMOXA-based antifouling coating on a fused-silica capillary inner surface, which was used to determine the bovine lactoferrin (Lf) in infant formula by capillary electrophoresis with ultraviolet (CE-UV) detection. The X-ray photoelectron spectroscopy (XPS) confirmed that PMOXA-r-GMA could be bonded onto fused-silica capillary inner wall and stable electroosmotic flow (EOF) was obtained in the PMOXA-r-GMA coated capillary at pH 2.2–9.0. The separation of a mixture of four basic proteins indicated that the PMOXA-r-GMA coated capillary exhibited excellent separation efficiency for the basic proteins. Therefore, the PMOXA-r-GMA coated capillary was used to determine the quantity of Lf in infant formula. Under the optimal conditions, the peak area (A) and the concentration of Lf showed a good linear relationship within the range of 10–500 μg/mL with a linear correlation coefficient of 0.9995. The limit of detection (LOD) and limit of quantitation (LOQ) for Lf were 5.0 μg/mL and 16.7 μg/mL, respectively. The recoveries at spiked concentrations of 0.20 and 0.40 mg/mL Lf in infant formula were 97.1±5.5% – 97.8±5.1%. The determined values of Lf in infant formula samples with Lf were consistent with the nominal values, indicating that our CE method could be successfully applied to the quantitative analysis of Lf in commercial infant formula.
研究领域:
分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
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2020
·
IF 2.888
Xingchuan Yang,Huanxin Li,Chunmei Cao,Li Xu,Guoji Liu
Liquid-liquid equilibria (LLE) data for ternary systems of water + 1,6-hexanediol + 1-pentanol/ 3-methyl-1-butanol/ 2-methyl-2-butanol were measured at (293.15, 303.15, 313.15 and 323.15) K under 101.3 kPa. Cloud-point method was applied to determine the solubility curves data. Distribution coefficient and separation factors for 1,6-hexanediol were calculated from the measured tie-line data to evaluate the extraction effect of alcohols on 1,6-hexanediol. The reliability of experimental LLE data were tested by Othmer–Tobias equation and Hand equation, NRTL and UNIQUAC models were applied to correlate the measured data and the calculated results show that both models can fit the experimental data very well.
研究领域:
化学工程
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热力学
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2022
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IF 3.841
Lina Guo,Xiaochao Chen,Ruijun Xie,Limin Han,Ning Zhu
4-Phenyl-2-(2′-pyridyl) quinoline ( L1 ) was synthesized by a three-component reaction of aniline, phenylacetylene and pyridine-2-aldehyde catalyzed by trifluoromethanesulfonic acid (TfOH). Its structure was confirmed by FT-IR, MS, NMR and X-ray single crystal diffraction analysis. L1 was investigated as a probe for the detection of Fe 3+ and Fe 2+ ions, which displayed fluorescence enhancement by Fe 3+ and fluorescence quenching by Fe 2+ . This unique fluorescence switching property of L1 was used to identify Fe 2+ and Fe 3+ with high selectivity and sensitivity. The detection limit of Fe 2+ was 8.772 × 10 −9 mol/L and that of Fe 3+ was 1.912 × 10 −9 mol/L. Mechanism investigation revealed that the fluorescence quenching of probe L1 was caused by ferrous-induced PET process and the fluorescence enhancement was due to the combinational effect of ferric-induced PCT enhancement and PET inhibition. In addition, the cyclic voltammetry curves demonstrated that both the Fe 2+ complexes and the Fe 3+ complexes displayed reversible redox process, indicating that the Fe 2+ and Fe 3+ complexes can be reversibly converted into each other upon electrical stimulation. The reversible “On” and “Off” of the fluorescence emission of iron complexes upon electrochemical stimulation could potentially be used in information recording and erasing.
研究领域:
分析化学
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材料科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
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IF 3.154
Lukai Ma,Guoqin Liu
A group of toxic aldehydes such as, malondialdehyde (MDA), 4-hydroxy-2-hexenal (HHE), and 4-hydroxy-2-nonenal (HNE) have been found in various vegetable oils and oil-based foods. Then simultaneous determination of them holds a great need in both the oil chemistry field and food field. In the present study, a simple and efficient analytical method was successfully developed for the simultaneous separation and detection of MDA, HHE, and HNE in vegetable oils by reversed-phase-high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detector (PAD) at dual-channel detection mode. The effect of various experimental factors on the extraction performance, such as coextraction solvent system, butylated hydroxytoluene addition, and trichloroacetic acid addition were systematically investigated. Results showed that the linear ranges were 0.02–10.00 μg/mL for MDA, 0.02–4.00 μg/mL for HHE, and 0.03–4.00 μg/mL for HNE with the satisfactory correlation coefficient of >0.999 for all detected aldehydes. The limit of detection (LOD) and limit of quantification (LOQ) of MDA, HHE, and HNE were ∼0.021and 0.020 μg/mL, ∼0.009 and 0.020 μg/mL, and ∼0.014 and 0.030 μg/mL, respectively. Their recoveries were 99.64–102.18%, 102.34–104.61%, and 98.87–103.04% for rapeseed oil and 96.38–98.05%, 96.19–101.34%, and 96.86–99.04% for French fries, separately. Under the selected conditions, the developed methods was successfully applied to the simultaneous determination of MDA, HHE, and HNE in different tested vegetable oils. The results indicated that this method could be employed for the quality assessment of vegetable oils.
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
Molecular Catalysis
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2020
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IF 3.687
Kaikai Liu,Tiefeng Wang,Minghan Han
The catalytic isomerization of 2-methyl-3-butenenitrile (2M3BN), a model reaction in the DuPont process, has been performed using NiL 4 (L=tri- O - p -tolyl phosphite) as a catalyst. The lowered catalytic activity in the isomerization with coexistence of 2-pentenenitrile (2PN) and 2-methyl-2-butenenitrile (2M2BN) indicates that both 2PN and 2M2BN are the catalyst inhibitors , and the quantitative relationship between the conversion of 2M3BN and the content of 2M2BN and 2PN is provided. DFT calculation results suggest that the inhibition effect is attributed to the generation of dead-end intermediates (2PN)NiL 2 and (2M2BN)NiL 2 , both of which take nickel atom out of the catalytic cycle in the isomerization process. To suppress the inhibition effect, new catalytic intermediates are rationally designed based on their computational %V bur . An efficient method that adding extra ligand 1, 5-bis(diphenylphosphino)pentane (dppp5) to the NiL 4 catalyst is selected experimentally. Compared to the results obtained with NiL 4 as catalyst, the (dppp5)NiL 2 increases the conversion of 2M3BN from 74.5 % to 93.4 % at 3 h of reaction and provides a high selectivity to 3PN (> 98 %) at optimal conditions.
研究领域:
催化
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有机化学
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计算化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Zhe Hou,Li-Fei Zhu,Xin-chi Yu,Ma-Qiang Sun,Fang Miao,Le Zhou
Twenty-two 2-aryl-9-methyl-3,4-dihydro-β-carbolin-2-ium bromides along with four 9-demethylated derivatives were synthesized and characterized by spectroscopic analysis. By using the mycelium growth rate method, the compounds were evaluated for antifungal activities in vitro against six plant pathogenic fungi, and structure–activity relationships (SAR) were derived. Almost all of the compounds showed obvious inhibition activity on each of the fungi at 150 μM. For all of the fungi, 10 of the compounds showed average inhibition rates of >80% at 150 μM, and most of their EC50 values were in the range of 2.0–30.0 μM. SAR analysis showed that the substitution pattern of the N-aryl ring significantly influences the activity; N9-alkylation improves the activity, whereas aromatization of ring-C reduces the activity. It was concluded that the present research provided a series of new 2-aryl-9-alkyl-3,4-dihydro-β-carbolin-2-iums with excellent antifungal potency and structure optimization design for the development of new carboline antifungal agents.
研究领域:
药理学
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有机化学
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植物保护学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR AND CELLULAR ENDOCRINOLOGY
·
2019
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IF 3.693
Wen Qi,Liting Zhou,Tianye Zhao,Shuang Ding,Qi Xu,Xu Han,Yaming Zhao,Xinyue Song,Tianyang Zhao,Xiaohan Zhang,Lin Ye
Background Mono-2-ethylhexyl phthalate (MEHP), an important metabolite of di (2-ethylhexyl) phthalate (DEHP), can induce lipid metabolic disorder . Previous studies have shown that MEHP promotes 3T3-L1 cell differentiation ; however, the underlying mechanism is unclear. The present study was performed to investigate the effect of the TYK-2/STAT-3 pathway on lipid accumulation induced by MEHP. Methods A 3T3-L1 precursor adipocyte differentiation model was exposed to MEHP. 3-Isobutyl-1-methylxanthine (IBMX), dexamethasone (DEX), and insulin were used to establish the 3T3-L1 precursor adipocyte differentiation model. Then the model cells were exposed to MEHP for 8 d. The lipid droplet formation in 3T3-L1 cells was determined with Oil-Red-O staining, and isopropyl alcohol was used to extract the lipid droplets for quantification. Flow cytometry was used to detect the intracellular reactive oxygen species (ROS) and mitochondrial membrane potential . Quantitative real-time polymerase chain reaction (qPCR) was used to detect mRNA expression, and western blotting was used to detect the expression of proteins encoded by TYK-2/STAT-3 pathway genes and adipogenesis-related genes. Results MEHP treatment , compared with the control treatment, significantly promoted the differentiation of 3T3-L1 cells and increased the expression of STAT-3 mRNA and protein and P-STAT3 protein in the cells. In addition, MEHP down-regulated the phosphorylation of STAT-3 in mitochondria. MEHP was found to influence the mitochondrial membrane potential and intracellular ROS levels. Conclusion MEHP may affect adipocyte differentiation and lead to lipid accumulation through the TYK-2/STAT-3 pathway.
研究领域:
毒理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENERGY
·
2023
·
IF 9
Xiaobin Zhou,Chao Liu,Jie Zhang,Yinming Fan,Yinian Zhu,Lihao Zhang,Shen Tang,Shengpeng Mo,Hongxiang Zhu,Zongqiang Zhu
Biphasic solvents are regarded as promising candidates for CO 2 capture but still suffer from the deficiency of inferior regenerability, which negatively affects their energy-saving potential. 2-Amino-2-methyl-1-propanol (AMP)-based absorbents have superior regenerability but poor phase-change performance. In this study, an effective strategy that using tetraethylenepentamine (TEPA) to regulate the phase change behavior of an AMP-pentamethyldiethylenetriamine (PMDETA) aqueous solution was proposed, aiming to develop a novel AMP-PMDETA-TEPA (A-P-T) biphasic solvent with good regenerability and excellent phase separation performance. The A-P-T biphasic solvent could realize a high CO 2 loading of 0.73 mol mol −1 and a high desorption efficiency of 75.3%. Its sensible heat requirement significantly decreased to 0.14 GJ·ton −1 CO 2 , 78.1% less than the monoethanolamine solution. The 13 C NMR characterization and quantum chemistry calculations indicated that with the introduction of TEPA, high polar TEPA-associated products were generated, which broke the original assimilation state of the A-P-T system and drove it to undergo phase change. Since the TEPA-associated products had a strong affinity to other CO 2 -captured products and H 2 O, they gathered together to form the CO 2 -rich phase. In contrast, less polar PMDETA showed a relatively weak affinity to the TEPA-associated products and was solely separated from the solution to form the CO 2 -lean phase.
研究领域:
化学工程
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环境科学
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能源技术
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产品关联
DOI
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Dandan Feng,Tianshu Zhang,Guan Zhou,Yanli Cao,Quan Luo,Huifang Xu,Xuefeng Lu
2′-Deoxy-2′-fluoroadenosine (2′-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2′-deoxy-2′-fluorouridine via one-pot transglycosylation catalyzed by a thymidine phosphorylase (TP) and a purine nucleoside phosphorylase (PNP). However, reliance on purified enzymes and low space-time yields present challenges for industrial application of the process. Here, we develop a whole-cell-based biocatalytic system employing TP and PNP from Escherichia coli , which demonstrates high catalytic efficiency and operational simplicity in scaled-up reaction. In particular, a thermal pretreatment of TP- and PNP-expressing whole cells, determined as 50 °C for 3 h, effectively suppressed endogenous deamination side reaction while enhancing 2′-F-dA yield. Subsequent optimization of enzyme and substrate loadings and their relative ratios achieved an unprecedented space-time yield of 1.22 g/L/h with 88.1 g/L product titer in a 500 mL scaled-up reaction, manifesting a highest total conversion of 68.2 %. An integrated purification process yielded gram-scale solid powder of 2′-F-dA with 98.0 % chemical purity and 85.0 % recovery. This novel whole-cell biocatalytic process demonstrates significant industrial potential for the production of 2′-F-dA.
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
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IF 2.487
Lingchen Yang,Zhiyong Zhao,Aibo Wu,Yifeng Deng,Zhenlei Zhou,Jianpeng Zhang,Jiafa Hou
A stable and sensitive method has been developed for use in food and livestock product safety for the detection of mycotoxins. This newly developed method allows for the determination of T-2 toxin, HT-2 toxin and diacetoxyscirpenol (DAS) in heart, liver, spleen, lung, kidney, Glandular stomach, muscular stomach, small intestine, muscle, bone and brain samples from broilers using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). The samples were initially extracted with ethyl acetate before being filtered through a 0.22 μm nylon syringe filter and subjected to chromatographic separation on a reversed-phase C18 (50 × 2.1 mm, 3 μm) column. A mobile phase composed of 0.1% acetic acid and 10 mM ammonium acetate in methanol and water was used in an assay of the levels of T-2 toxin, HT-2 toxin and DAS. For the analysis of the target compounds, the mass spectrometer was operated under positive electrospray ionization conditions in the selected reaction monitoring mode. The limit of detection was in the range of 0.02–0.05 ng/g, whereas the limit of quantification was in the range of 0.08–0.15 ng/g. The extraction recoveries of spiked samples from the high, intermediate and low levels ranged from 58.5% to 110.5%, and the relative standard deviation (RSD (%)) values were less than 17.0%. The results of inter- and intra-day precision (RSD (%)) were within 14.7%. The results revealed that the present method could be successfully applied to the analysis of T-2 toxin, HT-2 toxin and DAS in the real samples
研究领域:
食品安全
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分析化学
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畜牧学
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DOI
高影响因子
Journal of the American Chemical Society
·
2020
·
IF 14.612
Hai-Fang Li,Wenbo Cao,Xiaoxiao Ma,Xiaobo Xie,Yu Xia,Zheng Ouyang
The [2 + 2] photocycloaddition of alkenes and carbonyls is of fundamental interest and practical importance, as this process is extensively involved in oxetane-ring constructions. Although individual carbonyl group or alkene moiety has been utilized as photoactive species for oxetane formations upon ultraviolet photoexcitation, direct excitation of the entire noncovalent complex involving alkene and carbonyl substrates to achieve [2 + 2] photocycloadditions is rarely addressed. Herein, complexes with noncovalent interactions between benzophenone and C═C bonds in unsaturated lipids have been successfully characterized, and for the first time a [2 + 2] cycloaddition leading to the formation of oxetanes has been identified under visible-light irradiation. The mechanism of this reaction is distinctly different from the well-studied Paternò–Büchi reaction. The entire complexes characterized as dimeric proton-bonded alkene and carbonyl substrates can be excited under visible light, leading to electron transfer from the alkene moiety in fatty acyls to the carbonyl group within the complex. These results provide new insight into utilizing noncovalent complexes for the synthesis of oxetanes in which the excitation wavelength becomes independent of each individual substrate.
研究领域:
有机化学
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光化学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
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