MOLECULES
·
2024
·Vol. 29
·期 17
·3990
·
IF 4.2
Shuang Tian,Yinxu Zhao,Siqi Deng,Liman Hou,Juan Song,Ming Wang,Ming Bu
In the following study, a series of new lupeol-3-carbamate derivatives were synthesized, and the structures of all the newly derived compounds were characterized. The new compounds were screened to determine their anti-proliferative activity against human lung cancer cell line A549, human liver cancer cell line HepG2, and human breast cancer cell line MCF-7. Most of the compounds were found to show better anti-proliferative activity in vitro than lupeol. Among them, obvious anti-proliferation activity (IC50= 5.39~9.43 μM) was exhibited by compound3iagainst all three tumor cell lines. In addition, a salt reaction was performed on compound3k(IC50= 13.98 μM) and it was observed that the anti-proliferative activity and water solubility of compound3k·CH3I(IC50= 3.13 μM), were significantly enhanced subsequent to the salt formation process. The preliminary mechanistic studies demonstrated that apoptosis in HepG2 cells was induced by compound3k·CH3Ithrough the inhibition of the PI3K/AKT/mTOR pathway. In conclusion, a series of new lupeol-3-carbamate derivatives were synthesized via the structural modification of the C-3 site of lupeol, thus laying a theoretical foundation for the design of this new anticancer drug.
研究领域:
药理学
·
肿瘤学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
LETTERS IN APPLIED MICROBIOLOGY
·
2014
·Vol. 59
·期 3
·306-312
·
IF 1.749
Meng F.,Ma L.,Ji S.,Yang W.,Cao B.
Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY‐3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identified as Bacillus subtilis. The strain can produce cellulase when grown on various substrates, including carboxymethyl cellulose, rice straw, corn stover, soluble starch and wheat bran. The maximum cellulase activity of the strain was up to 4·323 ± 0·065 U ml−1 when cultivated in the medium containing corn stover (30 g l−1) for 24 h. The results demonstrated that corn stover is the most suitable substrate for cellulase production by the strain BY‐3. The crude cellulase of strain BY‐3 was most active at pH 5·5 and 60°C, and the enzyme in acetate buffer (50 mmol l−1) demonstrated a good stability at 60°C for at least 1 h. The crude cellulase exhibited a strong antibacterial activity against Staphylococcus aureus. The strain can be used in cost‐efficient cellulase production for bioconversion of agricultural residual biomass into biofuels.Significance and Impact of the StudyThe increased consumption of fossil fuels has caused serious energy crisis and environmental problem. Thus, an alternative energy source is necessary. Bioconversion of biomass, particularly agricultural residuals, into value‐added bioproducts, such as biofuels and chemical solvents, has received considerable attention. In this study, the newly isolated thermophilic Bacillus subtilis strain BY‐3 produces cellulase efficiently with the use of untreated corn stover as a sole carbon source. This strain possesses the thermostable cellulase that is active with diverse crop wastes with a broad pH range and is a highly promising candidate for agricultural waste management.
研究领域:
微生物学
·
生物技术
·
可再生能源
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 14
·1757-1765
·
IF 2.896
Can Zhang,Xiaoxiao Wu,Dongyang Li,Jinnuo Hu,Debin Wan,Zhen Zhang,Bruce D. Hammock
As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL−1 and 0.1 ng mL−1, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.
研究领域:
环境科学
·
分析化学
·
免疫学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2021
·Vol. 17
·期 4
·677-690
·
IF 4.099
Zhou Shuping,Ma Yongfang,Liu Xueke,Yu Pan,Huang Ning,Song Li,Xu Ruyue,Huo Zhen,Zhu Tao,Tang Xiaolong
The miR let-7b-5p (a kind of microRNAs) has many pathophysiological regulation effects, including human hepatocellular carcinoma (HCC) pathogenesis. This study investigated whether nanoparticle-mediated miR let-7b-5p could jointly enhance the therapeutic effect of sorafenib on HCC by
inhibiting the proliferation of HCC cells, inducing apoptosis, and reversing drug resistance. We evaluated the level of miR let-7b-5p in sorafenib-resistant HepG2 cells (HepG2R) and HepG2 HCC cells by qRT-PCR and analyzed the biological effects of hepatocellular carcinoma treated
with sorafenib with miR let-7b-5p, and further studied the toxicity of nanoparticles (Ab-miR-NPs) that deliver miR let-7b-5p mimics and target GPC3 on the surface of hepatocellular carcinoma cells. Results showed that, in HepG2 cells, the expression level of miR let-7b-5p was significantly
higher than that in HepG2R cells. Targeted nanoparticle Ab-miR-NPs mediated the delivery of miR let-7b-5p to the HCC cytoplasm and released miRNA after being broken down, down-regulating the expression of IGF1R and inhibiting AKT/mTOR and Ras/Raf signal transmission. Ab-miR-NPs
not only enhanced the proliferation of sorafenib in cultured HepG2R cells and induced cell apoptosis efficiency, but they also improved the anti-tumor activity in the mouse models. These results indicated that GPC3 antibody-modified PLGA-PLL (polylactic acid-glycolic acetic copolymer
grafted hyper-branched polylysine) loaded miR let-7b-5p polymer nanoparticles combined with sorafenib may be a new treatment strategy for HCC resistant to sorafenib.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SURFACTANTS AND DETERGENTS
·
2010
·Vol. 14
·期 3
·317-324
·
IF 0.795
Cui Zheng-gang,Song Hong-xing,Yu Jun-jie,Jiang Jian-zhong,Wang Feng
Alkali-surfactant-polymer (ASP) flooding has been considered to be one of the useful tertiary oil recovery techniques. However, field tests in China have revealed that serious side effects may occur due to using alkali. Thus alkali-free SP flooding is more favorable in China. Unfortunately, surfactants effective in ASP flooding are usually ineffective in the absence of alkali and new surfactants need to be designed. In this paper N -(3-Oxapropanoxyl)dodecanamide as a pure compound and a mixture of homologues with narrow EO number distribution, synthesized by the reaction of lauroyl chloride with diglycolamine and addition of one ethylene oxide to coconut monoethanolamide respectively, are examined for their adaptability in SP flooding. It is found that, when mixed with betaines, both products can reduce Daqing crude oil/connate water interfacial tension to a magnitude of 10 −3 –10 −4 mNm −1 at 45 °C in a wide surfactant concentration range, 0.01–0.5 wt%, and oil displacement tests using natural cores indicates that a tertiary oil recovery of 18.6 ± 0.4% OOIP can be achieved by SP flooding with the N -(3-Oxapropanoxyl)dodecanamide as the main surfactant without adding any alkaline agent and neutral electrolyte. N -(3-Oxapropanoxyl)dodecanamide, as a nonionic surfactant without a cloud point and producible industrially from renewable materials, is an ideal surfactant for SP flooding in the absence of an alkaline agent.
研究领域:
石油工程
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2023
·
IF 3.3
Yue-Ling Sun,Gang-Xiang Liu,Yi-Lei Lv,Liang Ma,Wen-Dong Yao,Ru-Ling Tang
Phosphate crystals are valuable materials according to their diverse structures and physical and chemistry properties. A zinc orthophosphate, (NH 4 ) 3 (H 3 O)Zn 4 (PO 4 ) 4 , has been obtained via hydrothermal route. It was crystallized in hexagonal space group P 6 3 , featuring a three-dimensional (3D) [Zn(PO 4 )] – anion network built by corner-sharing connected ZnO 4 and PO 4 tetrahedra. The NH 4 + and H 3 O + units are located in the six-member cavities of the framework as the balanced cations. (NH 4 ) 3 (H 3 O)Zn 4 (PO 4 ) 4 exhibits a moderate and phase matchable second harmonic generation effect (SHG) about 1.4 times of KH 2 PO 4 (KDP), which is the strongest in zinc orthophosphates. Besides structural features, optical properties measurements and theoretical analyses of (NH 4 ) 3 (H 3 O)Zn 4 (PO 4 ) 4 were performed.
研究领域:
材料科学
·
光学
·
晶体学
·
化学
✓ 已验证
DOI
高影响因子
CELLULAR AND MOLECULAR BIOLOGY
·
2023
·Vol. 69
·期 9
·
IF 1.6
Aili Xuan,Mei Yang,Qun Xia,Qian Sun
Asthma is a respiratory inflammatory disease, and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is involved in the progression of respiratory diseases. However, the role of NOX4 in asthma remains unclear. In the present study, we aimed to explore the effects of NOX4 on airway remodeling and inflammation. NOX4 expression was measured using immunocytochemistry (IHC), western blot, and real-time PCR (qPCR). Lung tissues were stained using the H&E assay. ELISA was used to examine the levels of airway remodeling-related indicators, and qPCR was used to detect airway inflammatory factors. The results indicated that NOX4 is highly expressed in lung tissues, bronchoalveolar lavage fluid (BALF), and serum of OVA-treated mice. Inhibition of NOX4 alleviated OVA-induced airway remodeling and inflammation. Similarly, TGF-β1 was also upregulated in BALF and serum OVA-induced mice. Inhibition of TGF-β1 signaling also improved airway remodeling and inflammation induced by OVA. Moreover, the downregulation of NOX4 inactivated the TGF-β1-Smad2/3 pathway, and TGF-β1 decreased Smad2/3 expression. Moreover, inhibition of the TGF-β1 was enhanced, while TGF-β1 reversed the effects on airway remodeling and inflammation induced by NOX4 inhibition. Taken together, the downregulation of NOX4 improves airway remodeling and inflammation via inactivation of the TGF-β1-Smad2/3 pathway in asthma mice, suggesting that NOX4 may be a therapeutic target for asthma.
研究领域:
呼吸系统疾病
·
分子生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Materials
·
2020
·
IF 2.705
Li Xinxi,Liu Long,Li Shumin,Guo Lin,Li Bin,Zhang Guoqing
Spinel lithium manganese oxide (LiMn2O4) based Li-ion battery (LIB) is attractive for hybrid/full electric vehicles because of its abundant resources and easy preparation. However, operation under an elevated temperature could cause severe capacity fading of the spinel cathodes. In this work, 1, 3-propane sultone (PS) is investigated as an electrolyte additive for improving the cyclability of the LiMn2O4/graphite LIB at elevated temperature. The charge and discharge measurement proves that PS can significantly enhance the cyclability of 053048-type LiMn2O4/graphite pouch cell at 60°C. Compared to the cell without additive, the capacity retention of the cell using electrolyte with 5% PS increases from 52 to 71% after 180 cycles. The improved cyclability is attributable to the modification of the solid electrolyte interface (SEI) on both positive and negative sides of the LiMn2O4/graphite cell by PS, which effectively prevents anode and cathode from structural breakdown and inhibits the electrolyte decomposition.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
Catalysts
·
2022
·Vol. 12
·期 7
·685
·
IF 4.501
Hao Dong,Cheng-Hua Xu,Fang-Lu Yang,Lei Du,Chen-Long Liu,Wen-Jing Chen,Lin Wang
Ca–Zr–Sn composite oxides catalysts for 1,4-butanediol (BDO) selective dehydration to 3-butene-1-ol (BTO) are synthesized by impregnation and co-precipitation in the present work. The results show that Ca–Zr–Sn catalysts prepared from co-precipitation by using NaOH-Na2CO3mixing alkali solution as precipitant exhibit an excellent catalytic property for BDO dehydration to BTO. For instance, Ca–Zr–Sn oxide with Ca/Zr and Sn/Zr molar ratio of 0.68 and 0.28 calcined at 650 °C gives a BDO conversion and BTO selectivity of about 97% and 82%, respectively, and exhibits no deactivation during 1000 h scale-up experimental testing. X-ray diffraction results indicate that catalytic active centers for BDO dehydration to BTO are from Ca0.15Zr0.85O crystal phase. NH3- and CO2-temperature programmed desorption prove that the surface of obtained catalysts can provide a large amount of acid and base sites simultaneously. FT-IR spectra of pyridine-adsorbed samples show that acid sites on the surface of Ca–Zr–Sn oxide catalyst mainly exist in a state of Lewis acid, which activates terminal -OH groups of BDO molecule through complexing. The activated -OH interacts with β-H activated on base sites O2−anions relative to Ca species, thereby the CH2=CH- bonds are produced through dehydration to form BTO.
研究领域:
化学工程
·
催化材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2017
·
IF 3.133
Zhangxing He,Yingqiao Jiang,Jing Zhu,Yuehua Li,Zhen Jiang,Huizhu Zhou,Wei Meng,Ling Wang,Lei Dai
In this paper, LiTi 2-x Sn x (PO 4 ) 3 /C (x = 0, 0.2, 0.3, 0.4) composites were synthesized by sol-gel approach, and were investigated as anodes for aqueous lithium ion battery . Sn doping with proper content has no obvious influence on phase and morphology of the composite, while excessive dosage of Sn can affect its crystal structure. Sn doping can enhance the electrochemical properties of LiTi 2 (PO 4 ) 3 /C (LC). Among LiTi 2-x Sn x (PO 4 ) 3 /C composites, LiTi 1.7 Sn 0.3 (PO 4 ) 3 /C (LC-Sn-0.3) demonstrates the best electrochemical properties. Most importantly, the rate performance of LC can be improved dramatically by Sn doping on Ti sites. LC-Sn-0.3 delivers a discharge capacity of 118.9, 115.3, and 104.2 mAh g −1 at 0.2, 6, and 20 C (1 C = 150 mA g −1 ), respectively, with significant increase of 15.2, 27.4, and 41.9 mAh g −1 compare with LC. In addition, LC-Sn-0.3 exhibits superior cycling performance. The capacity retention for LC-Sn-0.3 can keep 76.8% after 1000 cycles at relatively high rate of 10 C, while only 51.7% of initial discharge capacity is retained for LC. Our study reveals that Sn doping on Ti sites is an efficient and facile method to improve the performance of LiTi 2 (PO 4 ) 3 /C composite for aqueous lithium ion battery.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
DALTON TRANSACTIONS
·
2017
·Vol. 46
·期 32
·10630-10634
·
IF 4.029
Mei-Yan Gao,Shumei Chen,Lan-Xia Hu,Lei Zhang,Jian Zhang
Four titanium-oxo-clusters with cyclohex-3-ene-1-carboxylate (Cec) ligands, Ti6(μ2-O)2(μ3-O)2(Cec)4(OiPr)12 (PTC-91; HOiPr = isopropanol), Ti6(μ3-O)6(Cec)6(OiPr)6 (PTC-92 and PTC-93) and H2[Ti18(μ2-O)11(μ3-O)14(Cec)20(OiPr)4] (PTC-94), have been solvothermally synthesized under different conditions, respectively. These clusters with the same Cec ligand show totally different structures, and they are stable in water and other common solvents. The Ti6 clusters with different conformations are presented in PTC-91, PTC-92 and PTC-93, while PTC-94 is an unusual Ti18 cluster. The photocatalytic studies reveal that PTC-91 and PTC-94 have good H2 evolution ability.
研究领域:
材料化学
·
光催化
✓ 已验证
DOI
高影响因子
FITOTERAPIA
·
2019
·
IF 2.431
Yue Yang,Haihui Xie,Yueming Jiang,Xiaoyi Wei
Averrhoa carambola L. (Oxalidaceae) was widely cultivated for fruits (star fruit), whereas the value of leaves remains to be discovered. Our study on the leaves yielded five flavan-3-ols ( 1 – 5 ) and two 2-diglycosyloxybenzoates. Their structures were determined by spectroscopic and chemical methods. Epicatechin-(5,6-bc)-4β-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 1 ) and benzyl 2-β- d -apiofuranosyl-(1 → 6)-β- d -glucopyranosyloxybenzoate ( 6 ) were new structures. 6-( S −2 -Pyrrolidinone-5-yl)epicatechin ( 4 ) and 6-( R -2-pyrrolidinone-5-yl)epicatechin ( 5 ) were obtained as monomeric diastereomer for the first time and their absolute configurations were determined by electronic circular dichroism (ECD) computation. Epicatechin-(7,8-bc)-4α-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 2 ), epicatechin-(7,8-bc)-4β-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 3 ), and methyl 2-β- d -apiofuranosyl-(1 → 6)-β- d -glucopyranosyloxybenzoate ( 7 ) were not previously reported from the genus Averrhoa . Compounds 1 – 5 showed more potent 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) than l -ascorbic acid. Meanwhile 1 and 3 exhibited lipase and α-glucosidase inhibitory activities, respectively. The results clarified the structures of flavan-3-ols and 2-diglycosyloxybenzoates in the leaves and their antioxidant, lipase, and α-glucosidase inhibitory activities.
研究领域:
植物化学
·
药理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）